Status
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Gene information
General information
TC003493 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 5941376..5949651
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003493
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00575

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 90.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 5 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupation not fulfilled (died as prepupa) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupation delayed (still larva) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 5 (19 dpi)
    Days post injection


    remarks: later than schedule, hours 24
    Pupal morphology
    remarks: later than schedule, hours 24
    Adult morphology
    remarks: later than schedule, hours 24
    Fertility
    remarks: later than schedule, hours 24
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003493 RA
      MSTVERKEY QETKIMRTV SPVRNFRSS SQNLAEFDH 
      NLDYLLEDL QNSVSRPGS SLGTHRETS KSNSLNRFT 
      TVKASNPVT EYSSDDAYN YTSPDGTKR VTGYKKESY 
      SYSTSGGAP PAKAGIQNN INQLDSLLD DLQQVKNAS 
      FQEKESYNN TGGDPEFQR SGSKRTVQR ELHYGDTPR 
      SSSRSRTLE RTEREGSMT REIQYTNEG TYGDIRQIR 
      GRSPSPSSR TSTLSKQTK VKNVHSYPM EVVETTTPD 
      INPEILAHL DPSLHPPGN TKVTTTIKT YTYEIPGSG 
      DYPTNLNSS TETEKYVYS PNQTQSTPS KSFVYKKVE 
      NKSSENTVN YVPYQKPLG PVKETITTR SYQPGYIPD 
      APPTRNQTY VYNETTTTR HVDDSRGSL PRQQNPPRQ 
      DTYIKEVHE NNAYNRQYN DYPPPPQQH YIKEIHDTK 
      TTTNVRHPY PNGYPPNDK TVIYRHETH TTNNYGKPV 
      NDVESFDPK HPPYAHDRR PNEPVNIQY RYKSESTTT 
      NNYKAGYPP SDETQALLP KPFPTEHQP DGPPKRLDD 
      LMATIGHEP PNSPFNAGY NAHEQEMEQ KKKIETLKR 
      QQEEVDDAQ KKEPLQKSK NVTGPPVYY PPGHEMFAS 
      KEESGGAWR AEGGYQKAS GQYKYEAES KSKSKSSSG 
      ATVVPVCLP LCCGLPCAI I
    CDS sequence
    • >TC003493 RA
      atgtccacg gtcgaacga aaagaatac caagaaacg 
      aaaataatg cgtacggtg tcccccgtg cgcaacttc 
      cgcagctcg tcgcaaaac ctggccgaa ttcgaccac 
      aacctcgat tacctcctc gaagacctg cagaactcg 
      gtgtcgcgc cccggcagt tccctcgga actcaccgc 
      gaaacctca aaaagcaac tcccttaac cgcttcacc 
      accgtcaaa gcatcgaat cccgtaaca gaatactct 
      tccgacgac gcttacaat tacacgtcc cctgatggc 
      acgaaacgc gtcaccggt tacaagaaa gaatcctat 
      tcgtattcc acgagcggc ggagcccca cctgcgaaa 
      gcaggcatc caaaataac atcaaccaa ctggactcg 
      ctcttggac gacttgcaa caggtgaag aacgcatca 
      ttccaggaa aaagaatct tataataac actggcggt 
      gatccagaa tttcaaagg agcgggtcg aagaggaca 
      gtgcagcgc gaattgcac tatggggat actcccaga 
      tcgagctca aggagccgc actttggaa cggacggag 
      cgcgaaggg tcaatgaca agagaaatc caatacaca 
      aacgaaggt acttatggt gacatccgc caaatccgg 
      ggaaggtcc ccgtcccca tcatcccga acgtcaact 
      ttaagcaaa caaactaaa gtgaaaaac gtccatagc 
      taccccatg gaggttgtg gaaacgaca acccctgac 
      atcaacccc gagattttg gcccacctt gaccccagc 
      ctccaccct ccaggaaac accaaagtc accacaacc 
      atcaaaacc tacacctat gagatcccc ggctctggt 
      gactacccc acaaaccta aactcgtca acggaaaca 
      gaaaaatac gtttactcc ccgaaccaa acccaatca 
      acaccgagc aaaagcttt gtctataaa aaagtcgag 
      aacaaatcg tccgaaaac acagtcaac tacgtcccg 
      taccaaaaa ccgttgggc ccagttaag gagaccata 
      accacgagg tcgtaccaa cccggttac atccccgat 
      gcgcccccg acgcgcaac caaacgtac gtttacaac 
      gagacaacc accacccgg cacgttgac gactccagg 
      ggtagtttg ccgcggcag cagaacccc ccaagacaa 
      gacacgtac attaaggaa gtccacgaa aacaacgcg 
      tacaaccgg cagtacaac gattatccg ccaccgccg 
      cagcaacac tacattaaa gagatccat gacaccaaa 
      accaccacc aatgtccgt cacccctat cccaacgga 
      tatcccccg aatgacaag acggtcatt tacagacat 
      gagacacac acgactaat aattacggg aagccggtg 
      aatgatgtc gagagtttc gatcctaaa cacccgccg 
      tatgcacat gaccggagg ccgaacgaa ccggtgaat 
      atccagtac aggtacaag tcggaaagt acgacgact 
      aataattac aaagcgggg tatccccca agtgatgag 
      actcaagca ctgctccct aagcccttc cccacggaa 
      caccagccg gacgggccg ccgaaaaga ctggacgat 
      cttatggcg accatagga catgagccc cctaacagc 
      cccttcaat gcgggttac aacgcacac gagcaagaa 
      atggagcag aaaaagaaa attgaaacg ttaaagcgt 
      caacaagaa gaggttgat gacgcacaa aagaaggaa 
      cctcttcag aagagtaaa aacgtgaca ggtccgccg 
      gtttactac ccacctggc catgagatg tttgccagt 
      aaggaagaa agtggaggc gcttggaga gcagaaggc 
      ggctatcag aaagcatct ggtcaatat aaatacgaa 
      gcggaaagt aaaagtaaa tcaaaaagc agttctggc 
      gcgacagtt gtgccagtt tgtctaccc ttatgttgt 
      ggtctaccg tgtgccatt atttag
    mRNA sequence
    • >TC003493 RA
      aacatcgtc cagccaagt cgagttgat ctgagaata 
      gcacatgaa cgagtcttc gggtttacg aaacttgtg 
      ttgtgtgga tgtggtgtg gtacaggat acctggcga 
      cttcagcag gaaacgtat aatctgcaa gcagcatgt 
      ccacggtcg aacgaaaag aataccaag aaacgaaaa 
      taatgcgta cggtgtccc ccgtgcgca acttccgca 
      gctcgtcgc aaaacctgg ccgaattcg accacaacc 
      tcgattacc tcctcgaag acctgcaga actcggtgt 
      cgcgccccg gcagttccc tcggaactc accgcgaaa 
      cctcaaaaa gcaactccc ttaaccgct tcaccaccg 
      tcaaagcat cgaatcccg taacagaat actcttccg 
      acgacgctt acaattaca cgtcccctg atggcacga 
      aacgcgtca ccggttaca agaaagaat cctattcgt 
      attccacga gcggcggag ccccacctg cgaaagcag 
      gcatccaaa ataacatca accaactgg actcgctct 
      tggacgact tgcaacagg tgaagaacg catcattcc 
      aggaaaaag aatcttata ataacactg gcggtgatc 
      cagaatttc aaaggagcg ggtcgaaga ggacagtgc 
      agcgcgaat tgcactatg gggatactc ccagatcga 
      gctcaagga gccgcactt tggaacgga cggagcgcg 
      aagggtcaa tgacaagag aaatccaat acacaaacg 
      aaggtactt atggtgaca tccgccaaa tccggggaa 
      ggtccccgt ccccatcat cccgaacgt caactttaa 
      gcaaacaaa ctaaagtga aaaacgtcc atagctacc 
      ccatggagg ttgtggaaa cgacaaccc ctgacatca 
      accccgaga ttttggccc accttgacc ccagcctcc 
      accctccag gaaacacca aagtcacca caaccatca 
      aaacctaca cctatgaga tccccggct ctggtgact 
      accccacaa acctaaact cgtcaacgg aaacagaaa 
      aatacgttt actccccga accaaaccc aatcaacac 
      cgagcaaaa gctttgtct ataaaaaag tcgagaaca 
      aatcgtccg aaaacacag tcaactacg tcccgtacc 
      aaaaaccgt tgggcccag ttaaggaga ccataacca 
      cgaggtcgt accaacccg gttacatcc ccgatgcgc 
      ccccgacgc gcaaccaaa cgtacgttt acaacgaga 
      caaccacca cccggcacg ttgacgact ccaggggta 
      gtttgccgc ggcagcaga accccccaa gacaagaca 
      cgtacatta aggaagtcc acgaaaaca acgcgtaca 
      accggcagt acaacgatt atccgccac cgccgcagc 
      aacactaca ttaaagaga tccatgaca ccaaaacca 
      ccaccaatg tccgtcacc cctatccca acggatatc 
      ccccgaatg acaagacgg tcatttaca gacatgaga 
      cacacacga ctaataatt acgggaagc cggtgaatg 
      atgtcgaga gtttcgatc ctaaacacc cgccgtatg 
      cacatgacc ggaggccga acgaaccgg tgaatatcc 
      agtacaggt acaagtcgg aaagtacga cgactaata 
      attacaaag cggggtatc ccccaagtg atgagactc 
      aagcactgc tccctaagc ccttcccca cggaacacc 
      agccggacg ggccgccga aaagactgg acgatctta 
      tggcgacca taggacatg agcccccta acagcccct 
      tcaatgcgg gttacaacg cacacgagc aagaaatgg 
      agcagaaaa agaaaattg aaacgttaa agcgtcaac 
      aagaagagg ttgatgacg cacaaaaga aggaacctc 
      ttcagaaga gtaaaaacg tgacaggtc cgccggttt 
      actacccac ctggccatg agatgtttg ccagtaagg 
      aagaaagtg gaggcgctt ggagagcag aaggcggct 
      atcagaaag catctggtc aatataaat acgaagcgg 
      aaagtaaaa gtaaatcaa aaagcagtt ctggcgcga 
      cagttgtgc cagtttgtc tacccttat gttgtggtc 
      taccgtgtg ccattattt agaatgttc gctcacaat 
      tcaattgat aacagtgga aattaaagg tgccttaat 
      gttcatcat atacctcat caacttgtc agaggatta 
      tgtttatta atttatagt gttatgttt tttatttaa 
      cgttaattg tttattgta tataatttt tggtataag 
      cagctttaa attttgatg taaattcct gtactagga 
      ttaatacaa tgtattaaa acga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY