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Gene information
General information
TC003486 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid16: 1311687..1314265
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003486
    iBeetle screen iB_00574
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%
    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    maxilla not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    labium not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    labrum size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    prothorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003486 RA
      MSNNLEVEV VYFYARENS KSHKTDKFE LIYDEENPI 
      PIFRRGIKF TLALRFKNK SFDPQRDQL RLIFNFGPK 
      PNVIKGTRG VIKVTKERT PTNDFKFWG ANILDLSSD 
      LILEVWAPT SAPVGIWTL QVESSIVNS SIPPSVYNH 
      EGDFYILFN PWNCHDLVY MPEERLLDE YVLTDVGKI 
      WVGPYGSSR GREWVFGQF DACVLPAAM LMFEKSDLP 
      PASRGDPIK VSRMISKLV NSNDDDGVL VGRWDGEYD 
      DGTAPSAWT GSVQILQEF LDTQSPVSY GQCWVFSGV 
      VSTVCRALG IPSRVVSNL VSAHDANAS LTVDKYYNI 
      DNEEMDYDP NNPMGEDSI WNFHVWNDV WMARPDLPH 
      GYGGWQAID ATPQETSSG YYQCGPSSL EAIKKGQVG 
      FNYDVAFMV ASVNADVMR WKEDNTREL GFARIYSNK 
      YHVGRTILT KQAFIFDPN GDQDREDVT LQYKPPEGS 
      KAERLSLYN AVRGTELAK SFFALPDPG LEDVEFDLE 
      ELERIKIGE GFCVVVHMK NRSDKVRNI KALLSATSV 
      FYNGVKASL IKKEDIEVV LQPKSQETI RMTVKADEY 
      LEKLVEYSN LKLYAIASV QETRQTWTD EDDFEVLKP 
      KIDIRIPPE IPIGKPTPV TLRFVNPLK KILTNCRFN 
      LSGPTLIRN QVIRYSDVK PGGLVKINA DITPKIAGE 
      QILVATFSS KQLIDIRGS AKVEVLEDE E
    CDS sequence
    • >TC003486 RA
      atgagtaac aatctagaa gtagaagta gtctacttc 
      tacgcaagg gaaaattca aagtcgcac aaaacggac 
      aagtttgag ttgatatat gacgaagaa aatccaatc 
      ccaattttc agacgtggg attaaattt accctcgcg 
      ttgagattc aagaacaaa tcgtttgat ccacaaagg 
      gaccaattg cgtctaatc ttcaatttt ggacctaaa 
      ccaaacgtt attaaaggc acccgtggt gtgataaaa 
      gtaacgaaa gagagaacc cctactaac gatttcaaa 
      ttttggggg gcaaacatc ttagacctg tcctccgac 
      ttgattcta gaggtttgg gcccccact agtgccccc 
      gttggcata tggaccctc caagttgag tccagcatt 
      gttaactca agcattcct ccaagtgtc tacaaccac 
      gagggcgat ttctacatt ttattcaat ccttggaat 
      tgtcacgac ctcgtctac atgccagaa gagcggctc 
      ctggacgag tacgtcttg accgacgtt ggtaaaata 
      tgggtgggc ccatatggg tccagccgt ggtcgcgaa 
      tgggttttc ggccaattt gacgcctgt gtcctccct 
      gcagcgatg ctaatgttc gaaaaatcc gacttaccc 
      ccagctagt cgcggagac cccatcaaa gtcagcaga 
      atgatctca aaactggtc aatagtaac gacgatgat 
      ggggttttg gtgggccgt tgggacgga gaatacgac 
      gatgggaca gcaccatct gcctggact gggtctgtt 
      cagatcctg caggaattt ctggacacg caatccccc 
      gtgagctat gggcaatgc tgggtgttt tctggggtt 
      gtgtccaca gtttgtcgc gccttgggg atcccttca 
      agggttgtc tcgaacctg gtgtcagcc catgacgct 
      aacgcgtct ctcactgtt gacaaatac tacaacatt 
      gataacgag gagatggac tacgacccc aacaaccca 
      atgggtgag gattccatc tggaacttc cacgtctgg 
      aacgacgtg tggatggcg cgacctgac ctcccccat 
      gggtatggt gggtggcaa gctatcgat gccacaccg 
      caagaaact tcctctggt tattaccag tgcgggccg 
      tcgtctctg gaagccatc aaaaaggga caagttggg 
      ttcaattac gacgttgct ttcatggta gcgtcggtt 
      aatgccgat gtgatgcgt tggaaagag gataataca 
      agagaactc ggttttgct cgaatttat tccaacaaa 
      tatcatgtc gggcgtacg attttgact aaacaagcc 
      tttattttc gacccgaat ggggaccag gaccgggag 
      gatgttact ttgcagtac aaacctcca gaagggtca 
      aaagctgaa cgtttgtct ttgtacaat gctgttaga 
      ggcaccgaa cttgccaag agctttttt gctttgcct 
      gaccccggc ttggaggac gtggagttt gatctagaa 
      gaactagaa aggatcaaa atcggcgaa ggcttttgt 
      gtcgttgtt catatgaaa aatagaagt gataaggta 
      cgcaatatc aaagctctc ctttctgct accagtgtt 
      ttctacaat ggagttaaa gcaagtctc atcaagaag 
      gaagatatc gaagttgtt ttgcaacca aagtcgcag 
      gaaactata agaatgacc gtcaaagct gacgaatat 
      ttggaaaag ttggtcgag tatagcaac ctaaaactc 
      tatgccatt gcctcggtc caggagacg cgccaaacg 
      tggacggat gaagacgat ttcgaagtt ttgaagccc 
      aaaatagat ataaggatt cctcctgaa attcccatt 
      gggaaaccg acaccagtc actctacgt tttgtgaac 
      ccgctgaag aagattctg acaaattgt agatttaat 
      ctctctggc cccactttg attagaaat caggtcatt 
      cgttatagt gatgtaaaa ccaggaggt ttggtgaag 
      atcaacgcg gatatcaca ccgaaaatt gccggagaa 
      caaattcta gttgcaacc ttttcgtct aaacagctc 
      attgacatc cgaggatct gctaaagtc gaagttttg 
      gaagatgag gagtga
    mRNA sequence
    • >TC003486 RA
      taacagcga aaaaatcag tgcctgaaa gttataatc 
      caagatgag taacaatct agaagtaga agtagtcta 
      cttctacgc aagggaaaa ttcaaagtc gcacaaaac 
      ggacaagtt tgagttgat atatgacga agaaaatcc 
      aatcccaat tttcagacg tgggattaa atttaccct 
      cgcgttgag attcaagaa caaatcgtt tgatccaca 
      aagggacca attgcgtct aatcttcaa ttttggacc 
      taaaccaaa cgttattaa aggcacccg tggtgtgat 
      aaaagtaac gaaagagag aacccctac taacgattt 
      caaattttg gggggcaaa catcttaga cctgtcctc 
      cgacttgat tctagaggt ttgggcccc cactagtgc 
      ccccgttgg catatggac cctccaagt tgagtccag 
      cattgttaa ctcaagcat tcctccaag tgtctacaa 
      ccacgaggg cgatttcta cattttatt caatccttg 
      gaattgtca cgacctcgt ctacatgcc agaagagcg 
      gctcctgga cgagtacgt cttgaccga cgttggtaa 
      aatatgggt gggcccata tgggtccag ccgtggtcg 
      cgaatgggt tttcggcca atttgacgc ctgtgtcct 
      ccctgcagc gatgctaat gttcgaaaa atccgactt 
      acccccagc tagtcgcgg agaccccat caaagtcag 
      cagaatgat ctcaaaact ggtcaatag taacgacga 
      tgatggggt tttggtggg ccgttggga cggagaata 
      cgacgatgg gacagcacc atctgcctg gactgggtc 
      tgttcagat cctgcagga atttctgga cacgcaatc 
      ccccgtgag ctatgggca atgctgggt gttttctgg 
      ggttgtgtc cacagtttg tcgcgcctt ggggatccc 
      ttcaagggt tgtctcgaa cctggtgtc agcccatga 
      cgctaacgc gtctctcac tgttgacaa atactacaa 
      cattgataa cgaggagat ggactacga ccccaacaa 
      cccaatggg tgaggattc catctggaa cttccacgt 
      ctggaacga cgtgtggat ggcgcgacc tgacctccc 
      ccatgggta tggtgggtg gcaagctat cgatgccac 
      accgcaaga aacttcctc tggttatta ccagtgcgg 
      gccgtcgtc tctggaagc catcaaaaa gggacaagt 
      tgggttcaa ttacgacgt tgctttcat ggtagcgtc 
      ggttaatgc cgatgtgat gcgttggaa agaggataa 
      tacaagaga actcggttt tgctcgaat ttattccaa 
      caaatatca tgtcgggcg tacgatttt gactaaaca 
      agcctttat tttcgaccc gaatgggga ccaggaccg 
      ggaggatgt tactttgca gtacaaacc tccagaagg 
      gtcaaaagc tgaacgttt gtctttgta caatgctgt 
      tagaggcac cgaacttgc caagagctt ttttgcttt 
      gcctgaccc cggcttgga ggacgtgga gtttgatct 
      agaagaact agaaaggat caaaatcgg cgaaggctt 
      ttgtgtcgt tgttcatat gaaaaatag aagtgataa 
      ggtacgcaa tatcaaagc tctcctttc tgctaccag 
      tgttttcta caatggagt taaagcaag tctcatcaa 
      gaaggaaga tatcgaagt tgttttgca accaaagtc 
      gcaggaaac tataagaat gaccgtcaa agctgacga 
      atatttgga aaagttggt cgagtatag caacctaaa 
      actctatgc cattgcctc ggtccagga gacgcgcca 
      aacgtggac ggatgaaga cgatttcga agttttgaa 
      gcccaaaat agatataag gattcctcc tgaaattcc 
      cattgggaa accgacacc agtcactct acgttttgt 
      gaacccgct gaagaagat tctgacaaa ttgtagatt 
      taatctctc tggccccac tttgattag aaatcaggt 
      cattcgtta tagtgatgt aaaaccagg aggtttggt 
      gaagatcaa cgcggatat cacaccgaa aattgccgg 
      agaacaaat tctagttgc aaccttttc gtctaaaca 
      gctcattga catccgagg atctgctaa agtcgaagt 
      tttggaaga tgaggagtg aattagttt tagtttttt 
      gcacatttt tttgtatgt agtttagaa acttgcaca 
      cgtatgctt attctgaca cttttttac gcaatatac 
      tgattactg agaa