General information | |||
TC032380
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 5366449..5371239 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC032380 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_00572 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
larva shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (16 dpi)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
adult lethality - Number of animals showing the phenotype: 2 (19 dpi)
pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 2 (19 dpi)
previtellogenic egg chamber not present - Number of animals showing the phenotype: 4
Comment: prooocytes not present |
- >TC032380 RA
MGFTKEEKT VPYPKSVFF IVSNEFCER FSYYGMRTI LSLYLRDIL LFSESDSKI IYHTFAMFV YFFPIFGAI LSDSFLGKF RTILYVSIV YACGNVLLS LASAEPIGL PQIPFSMIG LLLIALGTG GIKPCVSAF GGDQFVLPQ QELQLASFF SMFYFSINA GSLISTFLT PVLREDVHC FDEDSCYPL AFAIPGILM IVSIVVFVL GKPLYKIRE PEGNVVVQV SKCIGHGIK QKFHSSDKV DHWLDRSEA KYGKTLVND IKVTLKVLV LFIPLPIFW ALYDQQGSG WTFQATRMD GYIGFYTIL PDQMQVINP LLILLFIPL FSYVVYPSL AKCNLLKTP LQRMVCGGL LTAASFAIS AGVAFALEA TYPVLPTEG NAQIRIYNP LQCDAKLTV LPHIDNQEI KSMGYTKLD ISGVQGSKL YDFKITGCD SITYTSGSN FNITEKEAL LYYMLPDEF VKLVDNIDK DEKGLPKIR TLVNPSKPD LNLTYWDSG KEGLTVGAH NNSLFTISP GTYKLENYN KELGFYLGG VYTVVVSLN DVNQVQKVE YYEITQPNS VHILWLIPQ YVVITAGEI MFSITGLEF SYSQAPISM KSVLQAAFL LTTAVGNLI IVIIESAKI FEKQSADFL LYTGLMVVD MIIFAFMAM RYKYIKKTE NSDNEELDQ SLQKKESNA IENPAFKHG DGDA
- >TC032380 RA
atgggcttc accaaggaa gaaaagact gtgccatac ccgaaatca gtattcttc attgtcagc aatgaattc tgcgaaagg ttcagctac tatggcatg cgaacaatt ctcagcttg tacttacgc gacatcctc ctcttctcc gaaagcgac tccaaaatc atctaccac accttcgca atgttcgtt tacttcttt ccaatcttc ggcgccatt ctctcggat agtttcctc ggaaaattc cgcacgatt ttatacgtt tcgatcgta tatgcttgc ggaaacgtg ctcctgtct ttagcttca gcagaacca atcggtcta ccacaaata cctttttcc atgatcggt ctgctactt atcgctctg ggaactgga ggaatcaaa ccctgcgta tcggcgttt ggaggagac cagtttgtt ttgccccaa caagaactc caattagcc tctttcttc tcaatgttc tacttctcc atcaatgct ggaagcttg atatcgacg tttctaacg ccggttttg agggaggat gtgcactgc tttgacgag gatagttgt tatcctctg gcgtttgcc atcccggga atactgatg attgtgtca atagttgtt ttcgtgctt ggcaaaccc ttgtataaa atcagagaa ccggaagga aatgtcgta gtccaagta tcgaaatgc attggacac gggattaag caaaagttc cactccagt gacaaagtc gaccactgg ttggaccgg tctgaagca aaatacggt aaaacccta gtcaacgat atcaaagta acgttgaaa gttctggtc ctgttcatc cccttgccc attttttgg gccctgtat gaccaacaa gggagcggc tggaccttc caagccacc agaatggac ggctatatc ggtttttac actatttta cccgaccaa atgcaagtg atcaaccct ctactgatc ctgctgttt atccctttg ttctcctac gtggtgtat ccctcactt gcaaaatgc aacttgctc aaaacaccg ctccagcgg atggtgtgt ggggggttg ttaacagcg gcttcgttt gcaatttcc gccggagtt gcctttgcc ttggaggcc acttatccc gtactgcca acggaagga aacgcccag atcaggatc tacaacccc cttcagtgc gatgccaaa ctgaccgtg ctaccgcac attgacaac caggaaatc aaatcgatg ggttatact aagttagac atcagtggt gttcaagga agcaagctt tatgatttc aaaataaca ggatgcgac tctattact tacacaagc ggaagtaac tttaacatc accgaaaaa gaagctctg ctctattac atgctacca gacgagttc gtcaaactt gtggacaat attgataaa gacgagaaa gggcttccg aaaattcga actttggtg aatccctca aaacccgat cttaacttg acttactgg gattcgggg aaagaaggt ttaactgtg ggtgcccat aacaactcg ttgttcacc atcagtcca gggacttac aagcttgaa aattacaac aaagaattg gggttttat cttggaggg gtttacaca gttgtggtt tcactgaac gacgtaaat caagtgcag aaagtggaa tattatgaa attacgcaa ccgaatagc gtccacatt ttgtggcta atcccccag tatgtcgtt atcaccgcg ggagaaata atgttctct ataaccgga ttggagttt tcgtattcc caagcccca atttccatg aaatcggtt ctccaagct gccttcctt ctaaccaca gccgttggt aatctcata atcgtcatt attgaatca gctaaaata tttgagaaa cagtctgct gattttcta ctatacact ggcttgatg gttgtcgat atgatcatt tttgctttt atggcaatg aggtacaag tacataaag aaaacggaa aacagtgat aacgaggaa ctggatcaa agtttgcag aagaaggaa agtaatgct atcgaaaat ccagccttc aaacatggt gatggtgat gcgtaa
- >TC032380 RA
ttggttcat tcattgttg ttagacggc cgcggtttg tcagtgcgc gtttattgt tgtacactt caacagaac gaaagtttg acacactga aacacattt caatctaaa tgtttctag tatgttgac aatttaaaa cgttagatg aaacagttt cccagtgaa tcatgataa gctagtgct atgatgtta gtcttcaat aactgtgat tgatttttg ataaaacat tgtgattaa gatgggctt caccaagga agaaaagac tgtgccata cccgaaatc agtattctt cattgtcag caatgaatt ctgcgaaag gttcagcta ctatggcat gcgaacaat tctcagctt gtacttacg cgacatcct cctcttctc cgaaagcga ctccaaaat catctacca caccttcgc aatgttcgt ttacttctt tccaatctt cggcgccat tctctcgga tagtttcct cggaaaatt ccgcacgat tttatacgt ttcgatcgt atatgcttg cggaaacgt gctcctgtc tttagcttc agcagaacc aatcggtct accacaaat acctttttc catgatcgg tctgctact tatcgctct gggaactgg aggaatcaa accctgcgt atcggcgtt tggaggaga ccagtttgt tttgcccca acaagaact ccaattagc ctctttctt ctcaatgtt ctacttctc catcaatgc tggaagctt gatatcgac gtttctaac gccggtttt gagggagga tgtgcactg ctttgacga ggatagttg ttatcctct ggcgtttgc catcccggg aatactgat gattgtgtc aatagttgt tttcgtgct tggcaaacc cttgtataa aatcagaga accggaagg aaatgtcgt agtccaagt atcgaaatg cattggaca cgggattaa gcaaaagtt ccactccag tgacaaagt cgaccactg gttggaccg gtctgaagc aaaatacgg taaaaccct agtcaacga tatcaaagt aacgttgaa agttctggt cctgttcat ccccttgcc cattttttg ggccctgta tgaccaaca agggagcgg ctggacctt ccaagccac cagaatgga cggctatat cggttttta cactatttt acccgacca aatgcaagt gatcaaccc tctactgat cctgctgtt tatcccttt gttctccta cgtggtgta tccctcact tgcaaaatg caacttgct caaaacacc gctccagcg gatggtgtg tggggggtt gttaacagc ggcttcgtt tgcaatttc cgccggagt tgcctttgc cttggaggc cacttatcc cgtactgcc aacggaagg aaacgccca gatcaggat ctacaaccc ccttcagtg cgatgccaa actgaccgt gctaccgca cattgacaa ccaggaaat caaatcgat gggttatac taagttaga catcagtgg tgttcaagg aagcaagct ttatgattt caaaataac aggatgcga ctctattac ttacacaag cggaagtaa ctttaacat caccgaaaa agaagctct gctctatta catgctacc agacgagtt cgtcaaact tgtggacaa tattgataa agacgagaa agggcttcc gaaaattcg aactttggt gaatccctc aaaacccga tcttaactt gacttactg ggattcggg gaaagaagg tttaactgt gggtgccca taacaactc gttgttcac catcagtcc agggactta caagcttga aaattacaa caaagaatt ggggtttta tcttggagg ggtttacac agttgtggt ttcactgaa cgacgtaaa tcaagtgca gaaagtgga atattatga aattacgca accgaatag cgtccacat tttgtggct aatccccca gtatgtcgt tatcaccgc gggagaaat aatgttctc tataaccgg attggagtt ttcgtattc ccaagcccc aatttccat gaaatcggt tctccaagc tgccttcct tctaaccac agccgttgg taatctcat aatcgtcat tattgaatc agctaaaat atttgagaa acagtctgc tgattttct actatacac tggcttgat ggttgtcga tatgatcat ttttgcttt tatggcaat gaggtacaa gtacataaa gaaaacgga aaacagtga taacgagga actggatca aagtttgca gaagaagga aagtaatgc tatcgaaaa tccagcctt caaacatgg tgatggtga tgcgtaatg gtagtgaag attgctcct gtggagtag aatatgtac ttcatgatt aatttattg atatgcaga actatttga gagcaattt gtgtaaata tgtatattt ttactagat actaaaaat aaataaatg atttaaata tat
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
