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Gene information
General information
TC032380
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC003455 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid16: 805162..809952
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC032380
    iBeetle screen iB_00572
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection

    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection

    Fertility
    egg production: number <50% of expected -
    previtellogenic egg chamber not present - Number of animals showing the phenotype: 4

    Comment: prooocytes not present
    vitellarium shortened - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032380 RA
      MGFTKEEKT VPYPKSVFF IVSNEFCER FSYYGMRTI 
LSLYLRDIL LFSESDSKI IYHTFAMFV YFFPIFGAI 
LSDSFLGKF RTILYVSIV YACGNVLLS LASAEPIGL 
PQIPFSMIG LLLIALGTG GIKPCVSAF GGDQFVLPQ 
QELQLASFF SMFYFSINA GSLISTFLT PVLREDVHC 
FDEDSCYPL AFAIPGILM IVSIVVFVL GKPLYKIRE 
PEGNVVVQV SKCIGHGIK QKFHSSDKV DHWLDRSEA 
KYGKTLVND IKVTLKVLV LFIPLPIFW ALYDQQGSG 
WTFQATRMD GYIGFYTIL PDQMQVINP LLILLFIPL 
FSYVVYPSL AKCNLLKTP LQRMVCGGL LTAASFAIS 
AGVAFALEA TYPVLPTEG NAQIRIYNP LQCDAKLTV 
LPHIDNQEI KSMGYTKLD ISGVQGSKL YDFKITGCD 
SITYTSGSN FNITEKEAL LYYMLPDEF VKLVDNIDK 
DEKGLPKIR TLVNPSKPD LNLTYWDSG KEGLTVGAH 
NNSLFTISP GTYKLENYN KELGFYLGG VYTVVVSLN 
DVNQVQKVE YYEITQPNS VHILWLIPQ YVVITAGEI 
MFSITGLEF SYSQAPISM KSVLQAAFL LTTAVGNLI 
IVIIESAKI FEKQSADFL LYTGLMVVD MIIFAFMAM 
RYKYIKKTE NSDNEELDQ SLQKKESNA IENPAFKHG 
DGDA
    CDS sequence
    • >TC032380 RA
      atgggcttc accaaggaa gaaaagact gtgccatac 
ccgaaatca gtattcttc attgtcagc aatgaattc 
tgcgaaagg ttcagctac tatggcatg cgaacaatt 
ctcagcttg tacttacgc gacatcctc ctcttctcc 
gaaagcgac tccaaaatc atctaccac accttcgca 
atgttcgtt tacttcttt ccaatcttc ggcgccatt 
ctctcggat agtttcctc ggaaaattc cgcacgatt 
ttatacgtt tcgatcgta tatgcttgc ggaaacgtg 
ctcctgtct ttagcttca gcagaacca atcggtcta 
ccacaaata cctttttcc atgatcggt ctgctactt 
atcgctctg ggaactgga ggaatcaaa ccctgcgta 
tcggcgttt ggaggagac cagtttgtt ttgccccaa 
caagaactc caattagcc tctttcttc tcaatgttc 
tacttctcc atcaatgct ggaagcttg atatcgacg 
tttctaacg ccggttttg agggaggat gtgcactgc 
tttgacgag gatagttgt tatcctctg gcgtttgcc 
atcccggga atactgatg attgtgtca atagttgtt 
ttcgtgctt ggcaaaccc ttgtataaa atcagagaa 
ccggaagga aatgtcgta gtccaagta tcgaaatgc 
attggacac gggattaag caaaagttc cactccagt 
gacaaagtc gaccactgg ttggaccgg tctgaagca 
aaatacggt aaaacccta gtcaacgat atcaaagta 
acgttgaaa gttctggtc ctgttcatc cccttgccc 
attttttgg gccctgtat gaccaacaa gggagcggc 
tggaccttc caagccacc agaatggac ggctatatc 
ggtttttac actatttta cccgaccaa atgcaagtg 
atcaaccct ctactgatc ctgctgttt atccctttg 
ttctcctac gtggtgtat ccctcactt gcaaaatgc 
aacttgctc aaaacaccg ctccagcgg atggtgtgt 
ggggggttg ttaacagcg gcttcgttt gcaatttcc 
gccggagtt gcctttgcc ttggaggcc acttatccc 
gtactgcca acggaagga aacgcccag atcaggatc 
tacaacccc cttcagtgc gatgccaaa ctgaccgtg 
ctaccgcac attgacaac caggaaatc aaatcgatg 
ggttatact aagttagac atcagtggt gttcaagga 
agcaagctt tatgatttc aaaataaca ggatgcgac 
tctattact tacacaagc ggaagtaac tttaacatc 
accgaaaaa gaagctctg ctctattac atgctacca 
gacgagttc gtcaaactt gtggacaat attgataaa 
gacgagaaa gggcttccg aaaattcga actttggtg 
aatccctca aaacccgat cttaacttg acttactgg 
gattcgggg aaagaaggt ttaactgtg ggtgcccat 
aacaactcg ttgttcacc atcagtcca gggacttac 
aagcttgaa aattacaac aaagaattg gggttttat 
cttggaggg gtttacaca gttgtggtt tcactgaac 
gacgtaaat caagtgcag aaagtggaa tattatgaa 
attacgcaa ccgaatagc gtccacatt ttgtggcta 
atcccccag tatgtcgtt atcaccgcg ggagaaata 
atgttctct ataaccgga ttggagttt tcgtattcc 
caagcccca atttccatg aaatcggtt ctccaagct 
gccttcctt ctaaccaca gccgttggt aatctcata 
atcgtcatt attgaatca gctaaaata tttgagaaa 
cagtctgct gattttcta ctatacact ggcttgatg 
gttgtcgat atgatcatt tttgctttt atggcaatg 
aggtacaag tacataaag aaaacggaa aacagtgat 
aacgaggaa ctggatcaa agtttgcag aagaaggaa 
agtaatgct atcgaaaat ccagccttc aaacatggt 
gatggtgat gcgtaa
    mRNA sequence
    • >TC032380 RA
      ttggttcat tcattgttg ttagacggc cgcggtttg 
tcagtgcgc gtttattgt tgtacactt caacagaac 
gaaagtttg acacactga aacacattt caatctaaa 
tgtttctag tatgttgac aatttaaaa cgttagatg 
aaacagttt cccagtgaa tcatgataa gctagtgct 
atgatgtta gtcttcaat aactgtgat tgatttttg 
ataaaacat tgtgattaa gatgggctt caccaagga 
agaaaagac tgtgccata cccgaaatc agtattctt 
cattgtcag caatgaatt ctgcgaaag gttcagcta 
ctatggcat gcgaacaat tctcagctt gtacttacg 
cgacatcct cctcttctc cgaaagcga ctccaaaat 
catctacca caccttcgc aatgttcgt ttacttctt 
tccaatctt cggcgccat tctctcgga tagtttcct 
cggaaaatt ccgcacgat tttatacgt ttcgatcgt 
atatgcttg cggaaacgt gctcctgtc tttagcttc 
agcagaacc aatcggtct accacaaat acctttttc 
catgatcgg tctgctact tatcgctct gggaactgg 
aggaatcaa accctgcgt atcggcgtt tggaggaga 
ccagtttgt tttgcccca acaagaact ccaattagc 
ctctttctt ctcaatgtt ctacttctc catcaatgc 
tggaagctt gatatcgac gtttctaac gccggtttt 
gagggagga tgtgcactg ctttgacga ggatagttg 
ttatcctct ggcgtttgc catcccggg aatactgat 
gattgtgtc aatagttgt tttcgtgct tggcaaacc 
cttgtataa aatcagaga accggaagg aaatgtcgt 
agtccaagt atcgaaatg cattggaca cgggattaa 
gcaaaagtt ccactccag tgacaaagt cgaccactg 
gttggaccg gtctgaagc aaaatacgg taaaaccct 
agtcaacga tatcaaagt aacgttgaa agttctggt 
cctgttcat ccccttgcc cattttttg ggccctgta 
tgaccaaca agggagcgg ctggacctt ccaagccac 
cagaatgga cggctatat cggttttta cactatttt 
acccgacca aatgcaagt gatcaaccc tctactgat 
cctgctgtt tatcccttt gttctccta cgtggtgta 
tccctcact tgcaaaatg caacttgct caaaacacc 
gctccagcg gatggtgtg tggggggtt gttaacagc 
ggcttcgtt tgcaatttc cgccggagt tgcctttgc 
cttggaggc cacttatcc cgtactgcc aacggaagg 
aaacgccca gatcaggat ctacaaccc ccttcagtg 
cgatgccaa actgaccgt gctaccgca cattgacaa 
ccaggaaat caaatcgat gggttatac taagttaga 
catcagtgg tgttcaagg aagcaagct ttatgattt 
caaaataac aggatgcga ctctattac ttacacaag 
cggaagtaa ctttaacat caccgaaaa agaagctct 
gctctatta catgctacc agacgagtt cgtcaaact 
tgtggacaa tattgataa agacgagaa agggcttcc 
gaaaattcg aactttggt gaatccctc aaaacccga 
tcttaactt gacttactg ggattcggg gaaagaagg 
tttaactgt gggtgccca taacaactc gttgttcac 
catcagtcc agggactta caagcttga aaattacaa 
caaagaatt ggggtttta tcttggagg ggtttacac 
agttgtggt ttcactgaa cgacgtaaa tcaagtgca 
gaaagtgga atattatga aattacgca accgaatag 
cgtccacat tttgtggct aatccccca gtatgtcgt 
tatcaccgc gggagaaat aatgttctc tataaccgg 
attggagtt ttcgtattc ccaagcccc aatttccat 
gaaatcggt tctccaagc tgccttcct tctaaccac 
agccgttgg taatctcat aatcgtcat tattgaatc 
agctaaaat atttgagaa acagtctgc tgattttct 
actatacac tggcttgat ggttgtcga tatgatcat 
ttttgcttt tatggcaat gaggtacaa gtacataaa 
gaaaacgga aaacagtga taacgagga actggatca 
aagtttgca gaagaagga aagtaatgc tatcgaaaa 
tccagcctt caaacatgg tgatggtga tgcgtaatg 
gtagtgaag attgctcct gtggagtag aatatgtac 
ttcatgatt aatttattg atatgcaga actatttga 
gagcaattt gtgtaaata tgtatattt ttactagat 
actaaaaat aaataaatg atttaaata tat