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Gene information
General information
TC032380
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC003455
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid16: 805162..809952
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032380
    iBeetle screen iB_00572
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    Fertility
    egg production: number <50% of expected -
    previtellogenic egg chamber not present - Number of animals showing the phenotype: 4

    Comment: prooocytes not present
    vitellarium shortened - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032380 RA
      MGFTKEEKT VPYPKSVFF IVSNEFCER FSYYGMRTI 
      LSLYLRDIL LFSESDSKI IYHTFAMFV YFFPIFGAI 
      LSDSFLGKF RTILYVSIV YACGNVLLS LASAEPIGL 
      PQIPFSMIG LLLIALGTG GIKPCVSAF GGDQFVLPQ 
      QELQLASFF SMFYFSINA GSLISTFLT PVLREDVHC 
      FDEDSCYPL AFAIPGILM IVSIVVFVL GKPLYKIRE 
      PEGNVVVQV SKCIGHGIK QKFHSSDKV DHWLDRSEA 
      KYGKTLVND IKVTLKVLV LFIPLPIFW ALYDQQGSG 
      WTFQATRMD GYIGFYTIL PDQMQVINP LLILLFIPL 
      FSYVVYPSL AKCNLLKTP LQRMVCGGL LTAASFAIS 
      AGVAFALEA TYPVLPTEG NAQIRIYNP LQCDAKLTV 
      LPHIDNQEI KSMGYTKLD ISGVQGSKL YDFKITGCD 
      SITYTSGSN FNITEKEAL LYYMLPDEF VKLVDNIDK 
      DEKGLPKIR TLVNPSKPD LNLTYWDSG KEGLTVGAH 
      NNSLFTISP GTYKLENYN KELGFYLGG VYTVVVSLN 
      DVNQVQKVE YYEITQPNS VHILWLIPQ YVVITAGEI 
      MFSITGLEF SYSQAPISM KSVLQAAFL LTTAVGNLI 
      IVIIESAKI FEKQSADFL LYTGLMVVD MIIFAFMAM 
      RYKYIKKTE NSDNEELDQ SLQKKESNA IENPAFKHG 
      DGDA
    CDS sequence
    • >TC032380 RA
      atgggcttc accaaggaa gaaaagact gtgccatac 
      ccgaaatca gtattcttc attgtcagc aatgaattc 
      tgcgaaagg ttcagctac tatggcatg cgaacaatt 
      ctcagcttg tacttacgc gacatcctc ctcttctcc 
      gaaagcgac tccaaaatc atctaccac accttcgca 
      atgttcgtt tacttcttt ccaatcttc ggcgccatt 
      ctctcggat agtttcctc ggaaaattc cgcacgatt 
      ttatacgtt tcgatcgta tatgcttgc ggaaacgtg 
      ctcctgtct ttagcttca gcagaacca atcggtcta 
      ccacaaata cctttttcc atgatcggt ctgctactt 
      atcgctctg ggaactgga ggaatcaaa ccctgcgta 
      tcggcgttt ggaggagac cagtttgtt ttgccccaa 
      caagaactc caattagcc tctttcttc tcaatgttc 
      tacttctcc atcaatgct ggaagcttg atatcgacg 
      tttctaacg ccggttttg agggaggat gtgcactgc 
      tttgacgag gatagttgt tatcctctg gcgtttgcc 
      atcccggga atactgatg attgtgtca atagttgtt 
      ttcgtgctt ggcaaaccc ttgtataaa atcagagaa 
      ccggaagga aatgtcgta gtccaagta tcgaaatgc 
      attggacac gggattaag caaaagttc cactccagt 
      gacaaagtc gaccactgg ttggaccgg tctgaagca 
      aaatacggt aaaacccta gtcaacgat atcaaagta 
      acgttgaaa gttctggtc ctgttcatc cccttgccc 
      attttttgg gccctgtat gaccaacaa gggagcggc 
      tggaccttc caagccacc agaatggac ggctatatc 
      ggtttttac actatttta cccgaccaa atgcaagtg 
      atcaaccct ctactgatc ctgctgttt atccctttg 
      ttctcctac gtggtgtat ccctcactt gcaaaatgc 
      aacttgctc aaaacaccg ctccagcgg atggtgtgt 
      ggggggttg ttaacagcg gcttcgttt gcaatttcc 
      gccggagtt gcctttgcc ttggaggcc acttatccc 
      gtactgcca acggaagga aacgcccag atcaggatc 
      tacaacccc cttcagtgc gatgccaaa ctgaccgtg 
      ctaccgcac attgacaac caggaaatc aaatcgatg 
      ggttatact aagttagac atcagtggt gttcaagga 
      agcaagctt tatgatttc aaaataaca ggatgcgac 
      tctattact tacacaagc ggaagtaac tttaacatc 
      accgaaaaa gaagctctg ctctattac atgctacca 
      gacgagttc gtcaaactt gtggacaat attgataaa 
      gacgagaaa gggcttccg aaaattcga actttggtg 
      aatccctca aaacccgat cttaacttg acttactgg 
      gattcgggg aaagaaggt ttaactgtg ggtgcccat 
      aacaactcg ttgttcacc atcagtcca gggacttac 
      aagcttgaa aattacaac aaagaattg gggttttat 
      cttggaggg gtttacaca gttgtggtt tcactgaac 
      gacgtaaat caagtgcag aaagtggaa tattatgaa 
      attacgcaa ccgaatagc gtccacatt ttgtggcta 
      atcccccag tatgtcgtt atcaccgcg ggagaaata 
      atgttctct ataaccgga ttggagttt tcgtattcc 
      caagcccca atttccatg aaatcggtt ctccaagct 
      gccttcctt ctaaccaca gccgttggt aatctcata 
      atcgtcatt attgaatca gctaaaata tttgagaaa 
      cagtctgct gattttcta ctatacact ggcttgatg 
      gttgtcgat atgatcatt tttgctttt atggcaatg 
      aggtacaag tacataaag aaaacggaa aacagtgat 
      aacgaggaa ctggatcaa agtttgcag aagaaggaa 
      agtaatgct atcgaaaat ccagccttc aaacatggt 
      gatggtgat gcgtaa
    mRNA sequence
    • >TC032380 RA
      ttggttcat tcattgttg ttagacggc cgcggtttg 
      tcagtgcgc gtttattgt tgtacactt caacagaac 
      gaaagtttg acacactga aacacattt caatctaaa 
      tgtttctag tatgttgac aatttaaaa cgttagatg 
      aaacagttt cccagtgaa tcatgataa gctagtgct 
      atgatgtta gtcttcaat aactgtgat tgatttttg 
      ataaaacat tgtgattaa gatgggctt caccaagga 
      agaaaagac tgtgccata cccgaaatc agtattctt 
      cattgtcag caatgaatt ctgcgaaag gttcagcta 
      ctatggcat gcgaacaat tctcagctt gtacttacg 
      cgacatcct cctcttctc cgaaagcga ctccaaaat 
      catctacca caccttcgc aatgttcgt ttacttctt 
      tccaatctt cggcgccat tctctcgga tagtttcct 
      cggaaaatt ccgcacgat tttatacgt ttcgatcgt 
      atatgcttg cggaaacgt gctcctgtc tttagcttc 
      agcagaacc aatcggtct accacaaat acctttttc 
      catgatcgg tctgctact tatcgctct gggaactgg 
      aggaatcaa accctgcgt atcggcgtt tggaggaga 
      ccagtttgt tttgcccca acaagaact ccaattagc 
      ctctttctt ctcaatgtt ctacttctc catcaatgc 
      tggaagctt gatatcgac gtttctaac gccggtttt 
      gagggagga tgtgcactg ctttgacga ggatagttg 
      ttatcctct ggcgtttgc catcccggg aatactgat 
      gattgtgtc aatagttgt tttcgtgct tggcaaacc 
      cttgtataa aatcagaga accggaagg aaatgtcgt 
      agtccaagt atcgaaatg cattggaca cgggattaa 
      gcaaaagtt ccactccag tgacaaagt cgaccactg 
      gttggaccg gtctgaagc aaaatacgg taaaaccct 
      agtcaacga tatcaaagt aacgttgaa agttctggt 
      cctgttcat ccccttgcc cattttttg ggccctgta 
      tgaccaaca agggagcgg ctggacctt ccaagccac 
      cagaatgga cggctatat cggttttta cactatttt 
      acccgacca aatgcaagt gatcaaccc tctactgat 
      cctgctgtt tatcccttt gttctccta cgtggtgta 
      tccctcact tgcaaaatg caacttgct caaaacacc 
      gctccagcg gatggtgtg tggggggtt gttaacagc 
      ggcttcgtt tgcaatttc cgccggagt tgcctttgc 
      cttggaggc cacttatcc cgtactgcc aacggaagg 
      aaacgccca gatcaggat ctacaaccc ccttcagtg 
      cgatgccaa actgaccgt gctaccgca cattgacaa 
      ccaggaaat caaatcgat gggttatac taagttaga 
      catcagtgg tgttcaagg aagcaagct ttatgattt 
      caaaataac aggatgcga ctctattac ttacacaag 
      cggaagtaa ctttaacat caccgaaaa agaagctct 
      gctctatta catgctacc agacgagtt cgtcaaact 
      tgtggacaa tattgataa agacgagaa agggcttcc 
      gaaaattcg aactttggt gaatccctc aaaacccga 
      tcttaactt gacttactg ggattcggg gaaagaagg 
      tttaactgt gggtgccca taacaactc gttgttcac 
      catcagtcc agggactta caagcttga aaattacaa 
      caaagaatt ggggtttta tcttggagg ggtttacac 
      agttgtggt ttcactgaa cgacgtaaa tcaagtgca 
      gaaagtgga atattatga aattacgca accgaatag 
      cgtccacat tttgtggct aatccccca gtatgtcgt 
      tatcaccgc gggagaaat aatgttctc tataaccgg 
      attggagtt ttcgtattc ccaagcccc aatttccat 
      gaaatcggt tctccaagc tgccttcct tctaaccac 
      agccgttgg taatctcat aatcgtcat tattgaatc 
      agctaaaat atttgagaa acagtctgc tgattttct 
      actatacac tggcttgat ggttgtcga tatgatcat 
      ttttgcttt tatggcaat gaggtacaa gtacataaa 
      gaaaacgga aaacagtga taacgagga actggatca 
      aagtttgca gaagaagga aagtaatgc tatcgaaaa 
      tccagcctt caaacatgg tgatggtga tgcgtaatg 
      gtagtgaag attgctcct gtggagtag aatatgtac 
      ttcatgatt aatttattg atatgcaga actatttga 
      gagcaattt gtgtaaata tgtatattt ttactagat 
      actaaaaat aaataaatg atttaaata tat