Status
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Gene information
General information
TC003450 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 5248827..5251790
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003450
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00571

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003450 RA
      MKQSWLLLL TLFYISSEV SSWRIFRNG RMVGGNLGE 
      PKCNCKESS IKEVQEEWF TQNLDHFNP TDETTWKQR 
      FYSNDQFFD PKNGGPVFL MIGGEGEAS IKWMTQGAW 
      VNYAEKFGA LMFQLEHRY YGKSHPTDD LSTQNLKYL 
      TSQQALADL ATFITAMNE KYSLPPDVK WIAFGGSYP 
      GSLAAWLRF KYPHLVHGA MSASGPLLA QVDFKDYFR 
      VIKESLATH SDDCVTAVQ QGVDQIGVL LKQEIGQAN 
      LNELFKLCD PVQNSINNE KDISNLYET IADDFAGVV 
      QYNKDNRVG SPAGANITI DVVCDIMVN QTIGPPVNR 
      LAKVNEVLL SAYDQKCLD YNYDKMINN LRNVSWDSE 
      ASEGGRQWT YQTCTEFGF YQTSDYEPQ IFGDQFSVD 
      FFIQQCTDI FGSIYDEDF LNSATERTN TYYGGLDIE 
      VSNVVFVHG SIDPWHALG ITKTIDEEA PAIYIEGTA 
      HCANMYPPA DTDLPQLKE AREQILNLI GTWLAQ
    CDS sequence
    • >TC003450 RA
      atgaaacag tcctggctg ctgttgttg actttattt 
      tacatttcc tcggaggtt tcttcttgg agaatcttc 
      cgcaatggt cgcatggtt ggtggtaac ctcggtgaa 
      ccaaaatgc aattgtaaa gaaagcagc ataaaagag 
      gtacaagag gaatggttt acacaaaat ttggaccat 
      tttaacccc acagacgag acaacttgg aaacaaaga 
      ttttacagt aatgatcaa ttttttgat ccgaaaaat 
      gggggccct gtctttttg atgataggg ggcgagggt 
      gaggcatcc atcaaatgg atgactcaa ggagcttgg 
      gttaattat gccgagaaa tttggggcc ctgatgttc 
      cagttggag cataggtat tacggcaag agtcacccc 
      actgacgac ttgagtacc caaaatctg aagtactta 
      acgagtcag caagctcta gccgacttg gcaactttc 
      ataactgca atgaacgaa aagtacagt ttaccgccc 
      gacgtaaaa tggatcgct ttcggcggt tcatatcca 
      ggttcatta gcggcttgg ctccgcttc aagtacccc 
      catttagtg cacggtgct atgtctgcc agtggtcct 
      ctactagct caagttgat tttaaagac tacttcaga 
      gtcattaag gaatcccta gcgactcac agcgacgac 
      tgcgtaacc gccgtccag caaggagtc gaccaaatc 
      ggcgtcttg ctcaagcaa gaaatcggc caagccaac 
      ctgaacgag ctcttcaaa ctgtgcgac ccggtccag 
      aactcgata aataacgag aaagacatt tcgaattta 
      tacgaaaca atcgctgat gatttcgcc ggggttgtt 
      cagtacaat aaagataac agggtaggc agccctgct 
      ggagcaaac attacaata gacgtagtt tgcgacata 
      atggtgaat cagacaata gggccgccg gttaatcgg 
      cttgcgaaa gtcaacgag gtgctgttg agcgcctac 
      gaccagaaa tgcctggat tataactac gacaaaatg 
      ataaataat ctgcggaat gtgagttgg gacagcgag 
      gcgtccgaa ggcggccgc cagtggacc taccagacg 
      tgcaccgag ttcggcttc taccaaacc tccgactac 
      gaaccgcag atattcggg gaccagttt tcggttgat 
      ttcttcatc cagcaatgt actgatatt ttcgggtcg 
      atctacgat gaggatttt cttaacagt gccacagag 
      cgcaccaat acgtactat gggggcctt gatattgaa 
      gtgagtaat gtggtgttt gtgcacggg tcgatcgac 
      ccgtggcat gctttaggt atcaccaaa actatcgac 
      gaggaagcg ccggctatt tatatcgaa ggaactgcc 
      cactgtgct aatatgtac cctccagct gatacggac 
      ttgcctcag ttgaaagaa gcgcgtgag cagatttta 
      aacctgatc ggcacgtgg ttggcgcag tga
    mRNA sequence
    • >TC003450 RA
      tcagtatga agtattttt aaaaagaag cggtccgct 
      actatttat ttattagtt taagtacgt cgataattt 
      ttgagtgtt attggagcc acattatca ctcactaat 
      tacaaacca tgaaacagt cctggctgc tgttgttga 
      ctttatttt acatttcct cggaggttt cttcttgga 
      gaatcttcc gcaatggtc gcatggttg gtggtaacc 
      tcggtgaac caaaatgca attgtaaag aaagcagca 
      taaaagagg tacaagagg aatggttta cacaaaatt 
      tggaccatt ttaacccca cagacgaga caacttgga 
      aacaaagat tttacagta atgatcaat tttttgatc 
      cgaaaaatg ggggccctg tctttttga tgatagggg 
      gcgagggtg aggcatcca tcaaatgga tgactcaag 
      gagcttggg ttaattatg ccgagaaat ttggggccc 
      tgatgttcc agttggagc ataggtatt acggcaaga 
      gtcacccca ctgacgact tgagtaccc aaaatctga 
      agtacttaa cgagtcagc aagctctag ccgacttgg 
      caactttca taactgcaa tgaacgaaa agtacagtt 
      taccgcccg acgtaaaat ggatcgctt tcggcggtt 
      catatccag gttcattag cggcttggc tccgcttca 
      agtaccccc atttagtgc acggtgcta tgtctgcca 
      gtggtcctc tactagctc aagttgatt ttaaagact 
      acttcagag tcattaagg aatccctag cgactcaca 
      gcgacgact gcgtaaccg ccgtccagc aaggagtcg 
      accaaatcg gcgtcttgc tcaagcaag aaatcggcc 
      aagccaacc tgaacgagc tcttcaaac tgtgcgacc 
      cggtccaga actcgataa ataacgaga aagacattt 
      cgaatttat acgaaacaa tcgctgatg atttcgccg 
      gggttgttc agtacaata aagataaca gggtaggca 
      gccctgctg gagcaaaca ttacaatag acgtagttt 
      gcgacataa tggtgaatc agacaatag ggccgccgg 
      ttaatcggc ttgcgaaag tcaacgagg tgctgttga 
      gcgcctacg accagaaat gcctggatt ataactacg 
      acaaaatga taaataatc tgcggaatg tgagttggg 
      acagcgagg cgtccgaag gcggccgcc agtggacct 
      accagacgt gcaccgagt tcggcttct accaaacct 
      ccgactacg aaccgcaga tattcgggg accagtttt 
      cggttgatt tcttcatcc agcaatgta ctgatattt 
      tcgggtcga tctacgatg aggattttc ttaacagtg 
      ccacagagc gcaccaata cgtactatg ggggccttg 
      atattgaag tgagtaatg tggtgtttg tgcacgggt 
      cgatcgacc cgtggcatg ctttaggta tcaccaaaa 
      ctatcgacg aggaagcgc cggctattt atatcgaag 
      gaactgccc actgtgcta atatgtacc ctccagctg 
      atacggact tgcctcagt tgaaagaag cgcgtgagc 
      agattttaa acctgatcg gcacgtggt tggcgcagt 
      gatatttgt gatatttac gttaaaata cgagtaact 
      aatgccatg tattgtacg taaagttta ccaaaataa 
      ataaatttt atgaaattt a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY