Status
Please wait ...
Gene information
General information
TC003446 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 5213417..5218190
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003446
    iBeetle screen iB_00570

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003446 RA
      MSLPEPLSK LFSEIDKNK QKFITNLRD AVAIQSVSA 
      WPHKRGEIR KMIEWARDR MQKLNIQTE IKELGKQTL 
      PDGRVIDLP PVVFGALGT DPKKKTVLV YGHLDVQPA 
      LKEDGWDSE PFVLTERSG KLYGRGSSD DKGPVLCWL 
      HAIECYQNL GIDIPVNVK FIFEGMEES GSVGLDELV 
      HSEKQKFLA NVDYVCISD NYWLGTKKP CITYGLRGV 
      CYFFIEVQC AAKDLHSGV FGGTVHEAM TDLIYLMGT 
      LVNKDGRIL VEGIYDAVA PLMEGEREI YKKIDFDVD 
      DYRSDVGCN RLLHNEQKE QILMHRWRY PSLSLHGIE 
      GAFAEPGAK TVIPGKVIG KFSIRIVPD QKPAQVEKC 
      VVAYLEKKW KQHGSPNTM KAYMTDGGN PWTEDPTHP 
      HYTAGLKAT KHVYKVEPD FTREGGSIP VTLTFQQAT 
      GKNVILLPV GAGDDGAHS QNEKIDIRN YIEGTKLLG 
      AYLYEVGQL Q
    CDS sequence
    • >TC003446 RA
      atgtctctt cccgaacca ttatcaaaa cttttcagt 
      gaaattgac aaaaacaag caaaagttc ataaccaac 
      ctccgcgat gccgttgcc attcagtcc gtgtcagcg 
      tggccccac aagcggggc gaaatccgc aaaatgatc 
      gagtgggcc agagaccgc atgcagaag ctcaacatc 
      caaacggaa atcaaggaa ttgggaaaa caaaccctg 
      cctgatggc agagttatt gacttgccc cctgtggtg 
      ttcggggcc ttgggcacc gatcctaag aagaagacc 
      gttctggtt tacgggcat ttggacgtt cagcctgcc 
      ctcaaggag gacggttgg gattccgag ccttttgtc 
      ctgactgaa aggtctgga aagttgtac ggaaggggg 
      tcatcggac gacaagggg ccagttttg tgctggtta 
      catgccatt gagtgttac cagaacttg gggattgat 
      attccagtc aatgtcaag tttattttt gagggaatg 
      gaggagtca ggaagtgtg gggttggac gagttggtg 
      cacagcgag aaacagaag tttttggca aatgttgac 
      tatgtttgc atttctgat aactactgg ttggggacg 
      aagaaaccc tgcatcact tatgggctc agaggggtc 
      tgctatttc ttcattgag gtgcaatgc gctgccaag 
      gacttgcat agtggggtc tttgggggc actgtccat 
      gaagcaatg actgatttg atttacctc atgggcact 
      ttggtcaac aaagacggc aggattctg gtggaaggc 
      atctacgac gccgtcgcc cccttgatg gagggcgaa 
      cgcgaaatt tacaaaaaa attgatttc gacgtggac 
      gattatcgc agtgacgtt ggttgcaac cgcctgtta 
      cacaatgag caaaaagag cagattttg atgcacaga 
      tggcgctac ccctccttg tccctccac ggcatcgag 
      ggggccttt gccgagcct ggcgccaaa accgtcatt 
      ccgggaaaa gtaatcggc aagttttcc atcagaata 
      gtccccgac cagaagccg gcccaagtt gaaaaatgc 
      gtggtggct tatttggag aagaagtgg aaacagcac 
      ggaagtccc aacacaatg aaggcgtac atgaccgat 
      ggtgggaac ccctggact gaggaccca acccaccct 
      cattacacc gcaggtctt aaggccacc aaacatgtg 
      tacaaggtc gagcctgat tttacccgt gagggcggc 
      tctattcct gttactcta acattccag caagctacg 
      ggaaagaac gttattctg ctgccggtt ggggcaggc 
      gatgacggg gctcattcg caaaacgag aaaatcgat 
      ataagaaat tatatcgaa ggaaccaag ctgttgggt 
      gcctatctt tacgaagtc ggacaattg cagtaa
    mRNA sequence
    • >TC003446 RA
      cagtggcta tagtacttc aaacacaaa accatattt 
      tattattaa caaccgtaa tctcctagt agagaccac 
      tgtctgaca ctgacaagt gacaataaa aggtttgac 
      cacccgtga aatcatcag aagttcagc cttcgtgga 
      caataataa taacctcaa tttaaattt gattgtcat 
      cccgctaac atgtctctt cccgaacca ttatcaaaa 
      cttttcagt gaaattgac aaaaacaag caaaagttc 
      ataaccaac ctccgcgat gccgttgcc attcagtcc 
      gtgtcagcg tggccccac aagcggggc gaaatccgc 
      aaaatgatc gagtgggcc agagaccgc atgcagaag 
      ctcaacatc caaacggaa atcaaggaa ttgggaaaa 
      caaaccctg cctgatggc agagttatt gacttgccc 
      cctgtggtg ttcggggcc ttgggcacc gatcctaag 
      aagaagacc gttctggtt tacgggcat ttggacgtt 
      cagcctgcc ctcaaggag gacggttgg gattccgag 
      ccttttgtc ctgactgaa aggtctgga aagttgtac 
      ggaaggggg tcatcggac gacaagggg ccagttttg 
      tgctggtta catgccatt gagtgttac cagaacttg 
      gggattgat attccagtc aatgtcaag tttattttt 
      gagggaatg gaggagtca ggaagtgtg gggttggac 
      gagttggtg cacagcgag aaacagaag tttttggca 
      aatgttgac tatgtttgc atttctgat aactactgg 
      ttggggacg aagaaaccc tgcatcact tatgggctc 
      agaggggtc tgctatttc ttcattgag gtgcaatgc 
      gctgccaag gacttgcat agtggggtc tttgggggc 
      actgtccat gaagcaatg actgatttg atttacctc 
      atgggcact ttggtcaac aaagacggc aggattctg 
      gtggaaggc atctacgac gccgtcgcc cccttgatg 
      gagggcgaa cgcgaaatt tacaaaaaa attgatttc 
      gacgtggac gattatcgc agtgacgtt ggttgcaac 
      cgcctgtta cacaatgag caaaaagag cagattttg 
      atgcacaga tggcgctac ccctccttg tccctccac 
      ggcatcgag ggggccttt gccgagcct ggcgccaaa 
      accgtcatt ccgggaaaa gtaatcggc aagttttcc 
      atcagaata gtccccgac cagaagccg gcccaagtt 
      gaaaaatgc gtggtggct tatttggag aagaagtgg 
      aaacagcac ggaagtccc aacacaatg aaggcgtac 
      atgaccgat ggtgggaac ccctggact gaggaccca 
      acccaccct cattacacc gcaggtctt aaggccacc 
      aaacatgtg tacaaggtc gagcctgat tttacccgt 
      gagggcggc tctattcct gttactcta acattccag 
      caagctacg ggaaagaac gttattctg ctgccggtt 
      ggggcaggc gatgacggg gctcattcg caaaacgag 
      aaaatcgat ataagaaat tatatcgaa ggaaccaag 
      ctgttgggt gcctatctt tacgaagtc ggacaattg 
      cagtaaact atacaaggt cagatttct caggcattg 
      cgctctttt tttataatc tatatcacg gaaaaatga 
      gcatatcaa tgagtgacg ccatgggtg taactattt 
      tatttgtct gggatttct gcatcaaaa aatatattt 
      ttatatgtt ccatttgct acaaatgga agtaaatag 
      gtaattgtt ttaaagacg cttaaaggg ttgccaata 
      ttgataata tgctaagat tctgatgtt tttataaga 
      actgatgta ataattaaa gctcaatca atatatgca 
      ataaaaatt tatacaatt a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY