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Gene information
General information
TC003438 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 5170397..5172146
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003438
    iBeetle screen iB_00569

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003438 RA
      MGCGCVIVT AIAVIFILK FVHKIKRFF KVPTVPYLE 
      ETWWGPRDK TEEDDSIQP FTVKVPDEV ITDLQQRLQ 
      NARPLTPPL EGVQHQYGI NTNLLKKIV DFWRNEYNW 
      KERETFLNK LPQFTVSVQ GLRLHYIHV KPEKTDGLK 
      VLPLLMLHG WPGSVREFY EIIPLLTKP QPGRDFVFE 
      VIAPSLPGY GFSEAAVRP GLGAIQMAV LFKNFMNRL 
      GFQKYYIQG GDWGAVITQ HMATLYPEK ILGLHSNMC 
      FINTLKSQV KLFLFSFYP TLIVKQEHV NKVYPLSSK 
      FAKTLLETG YMHLQATKP DTVGVALND SPIGLAAYI 
      LEKFITWTN PAWRDLEDG GLTKKYSYT SLLDNVMIY 
      WVTNSITTS MRLYAETFN KNQTKLGVA KIPTTVPTA 
      CARFSYDLA YSPTAVLKE KYKNIVYES DYDAGHFAA 
      FEEPELLAK DIYKGVEKI EQFHKNNER LS
    CDS sequence
    • >TC003438 RA
      atgggttgt ggttgtgtt attgtaact gccattgct 
      gttattttt atattgaaa tttgtacat aaaatcaaa 
      cggtttttt aaagtacct acagtgcca tatttggaa 
      gagacgtgg tggggacct agagacaaa accgaagaa 
      gatgattca attcaaccc ttcacagtt aaagtaccc 
      gatgaggtg ataactgat cttcaacaa agactacaa 
      aacgccagg ccactaacc ccgcctttg gaaggcgtt 
      caacaccag tacggaatc aacacaaac cttctcaag 
      aaaattgtt gacttctgg cgcaacgaa tacaattgg 
      aaggaacgc gaaaccttc ctcaacaaa ctcccccaa 
      tttaccgtc agcgtccaa ggcctacgc ctacactac 
      atccacgta aaacccgaa aaaaccgat ggtttgaag 
      gtccttccc ctattaatg ctgcacgga tggcctgga 
      tccgttcgg gaattttac gaaatcatc ccacttttg 
      accaagccc cagcctgga cgtgacttc gtcttcgaa 
      gtcatagca ccatcgctt cccggttat gggttttct 
      gaagctgcg gtacggcct gggcttggg gccattcaa 
      atggcagtg ttgttcaaa aattttatg aatcggctt 
      ggtttccaa aagtattac atccaagga ggggactgg 
      ggggctgtt attacacaa cacatggca actttatac 
      ccggaaaaa atcctgggt ctccactca aatatgtgt 
      tttatcaat acgctaaag tctcaggtt aaattgttc 
      ctgttcagt ttttatccg actttgatt gtgaagcaa 
      gagcacgtc aataaggtg taccctttg tcgtcaaaa 
      ttcgcaaaa acgttgctg gaaactggc tacatgcat 
      ttgcaagcg acaaaacct gataccgtt ggggtggct 
      cttaatgat tctccaatc ggtctagcc gcttatatt 
      ttggaaaaa ttcataact tggaccaac ccagcgtgg 
      agggacttg gaagatggt ggtttaact aaaaaatat 
      tcctacact agtctactg gataacgta atgatttac 
      tgggtgacc aattcgata acaacttcc atgcgattg 
      tatgcggaa actttcaac aaaaaccaa accaaactc 
      ggagttgca aaaattccg accactgtt ccgacagct 
      tgtgccaga ttttcgtac gatctagca tacagtcca 
      actgctgtt ttgaaggaa aaatacaaa aatatcgtg 
      tatgagtcg gattatgat gcaggtcat ttcgcagct 
      ttcgaagaa ccggagctt ttggctaaa gatatttac 
      aaaggggtt gaaaaaatc gagcagttt cacaaaaac 
      aatgagcgt ttaagttga
    mRNA sequence
    • >TC003438 RA
      agttggaac aaaataata ttcatatct acttgcaat 
      caacacctg aaggttgtt gcttgtgac taaaatacc 
      tagttgtag taaaatatt acgtattta caatgggtt 
      gtggttgtg ttattgtaa ctgccattg ctgttattt 
      ttatattga aatttgtac ataaaatca aacggtttt 
      ttaaagtac ctacagtgc catatttgg aagagacgt 
      ggtggggac ctagagaca aaaccgaag aagatgatt 
      caattcaac ccttcacag ttaaagtac ccgatgagg 
      tgataactg atcttcaac aaagactac aaaacgcca 
      ggccactaa ccccgcctt tggaaggcg ttcaacacc 
      agtacggaa tcaacacaa accttctca agaaaattg 
      ttgacttct ggcgcaacg aatacaatt ggaaggaac 
      gcgaaacct tcctcaaca aactccccc aatttaccg 
      tcagcgtcc aaggcctac gcctacact acatccacg 
      taaaacccg aaaaaaccg atggtttga aggtccttc 
      ccctattaa tgctgcacg gatggcctg gatccgttc 
      gggaatttt acgaaatca tcccacttt tgaccaagc 
      cccagcctg gacgtgact tcgtcttcg aagtcatag 
      caccatcgc ttcccggtt atgggtttt ctgaagctg 
      cggtacggc ctgggcttg gggccattc aaatggcag 
      tgttgttca aaaatttta tgaatcggc ttggtttcc 
      aaaagtatt acatccaag gaggggact ggggggctg 
      ttattacac aacacatgg caactttat acccggaaa 
      aaatcctgg gtctccact caaatatgt gttttatca 
      atacgctaa agtctcagg ttaaattgt tcctgttca 
      gtttttatc cgactttga ttgtgaagc aagagcacg 
      tcaataagg tgtaccctt tgtcgtcaa aattcgcaa 
      aaacgttgc tggaaactg gctacatgc atttgcaag 
      cgacaaaac ctgataccg ttggggtgg ctcttaatg 
      attctccaa tcggtctag ccgcttata ttttggaaa 
      aattcataa cttggacca acccagcgt ggagggact 
      tggaagatg gtggtttaa ctaaaaaat attcctaca 
      ctagtctac tggataacg taatgattt actgggtga 
      ccaattcga taacaactt ccatgcgat tgtatgcgg 
      aaactttca acaaaaacc aaaccaaac tcggagttg 
      caaaaattc cgaccactg ttccgacag cttgtgcca 
      gattttcgt acgatctag catacagtc caactgctg 
      ttttgaagg aaaaataca aaaatatcg tgtatgagt 
      cggattatg atgcaggtc atttcgcag ctttcgaag 
      aaccggagc ttttggcta aagatattt acaaagggg 
      ttgaaaaaa tcgagcagt ttcacaaaa acaatgagc 
      gtttaagtt gaaaaatat tgcatggac gatggaata 
      ttttcttct gttaataaa gaatgttat tttaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY