Status
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Gene information
General information
TC003425 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 4881867..4915241
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003425
    iBeetle screen iB_00567

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003425 RA
      MDDPEIQET VESILGKGA KVVDCEKKS SIKEEEVLF 
      INGMPVTLE GRDGLAIKQ ALITGQVPP CDLLNTLLI 
      KTGILKAPV KLDTSLSVK STTVTKEEV TVSRDGKVV 
      DERSRETKE QNFYTSAIT EVYEPIKII PTAELLNGK 
      YARTKYSDN NGYETGSEG KFESSSSAS SCESDGPTY 
      KPQTCLSKD SGHLSNSTS TNKADFTPS SADSLSSLD 
      EPDFLSEQF RKCTFNGTN GFRKDFTSL PQTPQTKSF 
      STVYNGEAV QENATDTAL QPDNALVYR DGNLLSGSL 
      EALIQHMVP TDVYYPDRA YLFAFLLSS RLFIKPHDL 
      LARVRSLCE TQQGLGSAN GGASHPGQA RFAEHLVQL 
      LAEWTETFP YDFRDERVM QHVRTITQQ CCAINPSIR 
      GNVSSLLHN LLQRLTALE QYEKTLDLS AQPQDENCT 
      DISEVCPVP AVLAQQLTH VELERLSFI GPEEFVQAF 
      AKENPHLET SFKDMKKTH NLEQYVQWF NRLSYFVAT 
      QVCKYQKKK QRVRVVEYW IETGRECFN IGNFNSLMA 
      IIAGLNMSP ISRLKKTWN KIHSGKFAI LEHQMDPSS 
      NFSSYRSTL KAAMWRSHG AKDQRQRIV IPFFSLLVK 
      DLYFLNQGC SNKLPNGHI NFEKFWQLA KQVTEFMAW 
      KQVTCPFEK DPKVISVLQ HGPILNENA LALASFDCE 
      PPENNQEKE RCKTLKADS TAM
    CDS sequence
    • >TC003425 RA
      atggacgac ccggaaatc caggaaacg gtcgaatcg 
      atcctgggc aagggcgcc aaagtggtc gattgcgag 
      aaaaagtcg tccatcaag gaggaagaa gtgttgttc 
      atcaacggc atgcccgtc accctcgag ggcagggac 
      ggcctggcc ataaagcag gccctaatc acgggccag 
      gtgccccct tgcgacctc ctcaacacc ctcctgatc 
      aaaaccggc atcctcaaa gcccccgtc aaactcgac 
      acctcgctc agcgtcaaa tccaccaca gtgaccaag 
      gaggaggtc acggtgtcc agagacggg aaggtcgtg 
      gacgagcgc agtcgcgag accaaggag cagaacttc 
      tacaccagc gccatcacc gaggtctac gagcccatc 
      aagatcatc cccacggcg gagctcctc aacggcaag 
      tacgccagg accaagtac tcggacaac aacggctac 
      gagactgga agtgaagga aaattcgag tcgagctcg 
      agcgcctcc agctgcgag tcggacggg ccgacctac 
      aagccccag acctgcctg agcaaggac tcgggccac 
      ctgagcaac tccacgtcc accaacaag gcggacttc 
      acgcccagc tcggcggac tcgctcagt tcgctcgac 
      gaacccgat tttttgagc gagcagttc aggaagtgc 
      acgttcaat gggactaac ggttttcgc aaggatttt 
      accagcttg ccacaaact cctcaaact aaaagtttc 
      tcgactgtc tacaacggc gaggcagta caggaaaac 
      gccacagat accgccttg cagccagat aatgccttg 
      gtctaccgc gatggcaac ttgctgtct ggctccctt 
      gaagcttta atccagcac atggttcca accgacgtc 
      tactaccca gacagggcc tacctattt gcctttcta 
      ttgtcttca cgcctattc attaagccc cacgatttg 
      cttgccaga gttcggagt ttgtgtgag acgcagcaa 
      ggactgggg agtgcaaac ggcggcgct tcccatcca 
      ggacaggcc cgatttgcc gaacactta gtacaattg 
      cttgcagaa tggaccgag acgttccct tacgacttt 
      agggatgag agggtcatg cagcatgtc cggacaata 
      acacagcag tgttgcgcc ataaatccc agtattcgg 
      ggaaacgtt tcgtccctc ctgcacaat cttcttcag 
      aggttgacc gcgctagaa cagtacgaa aaaacgctg 
      gatttgtcg gcgcaaccg caagatgaa aactgcacg 
      gatatttcg gaagtttgt ccagtgccg gcggttctt 
      gcacaacaa ctcacacac gttgaactt gaaaggctt 
      tcgttcatc ggccccgag gagtttgtg caagctttc 
      gcgaaggag aatccgcat ttggagacg tcgttcaaa 
      gatatgaag aagacgcac aatttggaa caatacgtg 
      caatggttt aatagactg agctatttt gtagcgacg 
      caagtttgt aaataccag aagaaaaaa cagcgagtg 
      cgcgtggtg gaatactgg attgaaaca ggccgtgag 
      tgcttcaac atcggcaat ttcaacagt ctgatggcc 
      atcatcgcc ggcctaaat atgtcaccg atttcaagg 
      ctaaagaaa acttggaac aaaatccac tctggtaaa 
      ttcgccata ctcgagcac caaatggac cccagcagt 
      aactttagt agctacaga agcacgctt aaagccgcc 
      atgtggcgc agccacggc gccaaagac cagcgacag 
      agaatcgtg atcccgttc tttagtttg ctggtcaag 
      gatttgtat ttcctcaac caggggtgt tccaataag 
      cttccaaat ggccacata aattttgaa aagttctgg 
      cagttggcg aaacaggtc acggagttt atggcctgg 
      aagcaggtg acgtgtccg ttcgagaag gaccccaaa 
      gtcatttca gttctgcaa catggacca attttaaac 
      gagaacgcg ctcgcgctc gccagcttc gactgcgag 
      cccccggag aacaaccag gagaaggag aggtgcaag 
      acgctcaaa gccgacagc actgcgatg taa
    mRNA sequence
    • >TC003425 RA
      agtaacagg agccagccc gccatcggc ctgaaacac 
      acaccaaaa tcaagggta agtcaccca ataatttcc 
      ttcccaaat tcgcaacgg tcacaaaat gattcaaaa 
      agaaaatcc ctctccgac tccatcagc gcaggccgc 
      gtctgtaca atcgcaatg gcggcgact gtacaaaat 
      gactcattc cgtcagttt cacggtgtc tgtcggaac 
      gtacgcacg tagtgttcc gttcttcta tgtttgtaa 
      acaacatat gaccaccaa agcgcaatt tttgcgttt 
      tttggcgaa aaaatcgac gcgattttg tattttccc 
      gctcagtag tcacgaaac aattgtgtt gtgttcgtt 
      tgtttgacc tgtgttgac tatgattgg gacgttcgg 
      tttgtggta attgtgctt caagagcgg cgcaaatcc 
      taagccgca acatggacg acccggaaa tccaggaaa 
      cggtcgaat cgatcctgg gcaagggcg ccaaagtgg 
      tcgattgcg agaaaaagt cgtccatca aggaggaag 
      aagtgttgt tcatcaacg gcatgcccg tcaccctcg 
      agggcaggg acggcctgg ccataaagc aggccctaa 
      tcacgggcc aggtgcccc cttgcgacc tcctcaaca 
      ccctcctga tcaaaaccg gcatcctca aagcccccg 
      tcaaactcg acacctcgc tcagcgtca aatccacca 
      cagtgacca aggaggagg tcacggtgt ccagagacg 
      ggaaggtcg tggacgagc gcagtcgcg agaccaagg 
      agcagaact tctacacca gcgccatca ccgaggtct 
      acgagccca tcaagatca tccccacgg cggagctcc 
      tcaacggca agtacgcca ggaccaagt actcggaca 
      acaacggct acgagactg gaagtgaag gaaaattcg 
      agtcgagct cgagcgcct ccagctgcg agtcggacg 
      ggccgacct acaagcccc agacctgcc tgagcaagg 
      actcgggcc acctgagca actccacgt ccaccaaca 
      aggcggact tcacgccca gctcggcgg actcgctca 
      gttcgctcg acgaacccg attttttga gcgagcagt 
      tcaggaagt gcacgttca atgggacta acggttttc 
      gcaaggatt ttaccagct tgccacaaa ctcctcaaa 
      ctaaaagtt tctcgactg tctacaacg gcgaggcag 
      tacaggaaa acgccacag ataccgcct tgcagccag 
      ataatgcct tggtctacc gcgatggca acttgctgt 
      ctggctccc ttgaagctt taatccagc acatggttc 
      caaccgacg tctactacc cagacaggg cctacctat 
      ttgcctttc tattgtctt cacgcctat tcattaagc 
      cccacgatt tgcttgcca gagttcgga gtttgtgtg 
      agacgcagc aaggactgg ggagtgcaa acggcggcg 
      cttcccatc caggacagg cccgatttg ccgaacact 
      tagtacaat tgcttgcag aatggaccg agacgttcc 
      cttacgact ttagggatg agagggtca tgcagcatg 
      tccggacaa taacacagc agtgttgcg ccataaatc 
      ccagtattc ggggaaacg tttcgtccc tcctgcaca 
      atcttcttc agaggttga ccgcgctag aacagtacg 
      aaaaaacgc tggatttgt cggcgcaac cgcaagatg 
      aaaactgca cggatattt cggaagttt gtccagtgc 
      cggcggttc ttgcacaac aactcacac acgttgaac 
      ttgaaaggc tttcgttca tcggccccg aggagtttg 
      tgcaagctt tcgcgaagg agaatccgc atttggaga 
      cgtcgttca aagatatga agaagacgc acaatttgg 
      aacaatacg tgcaatggt ttaatagac tgagctatt 
      ttgtagcga cgcaagttt gtaaatacc agaagaaaa 
      aacagcgag tgcgcgtgg tggaatact ggattgaaa 
      caggccgtg agtgcttca acatcggca atttcaaca 
      gtctgatgg ccatcatcg ccggcctaa atatgtcac 
      cgatttcaa ggctaaaga aaacttgga acaaaatcc 
      actctggta aattcgcca tactcgagc accaaatgg 
      accccagca gtaacttta gtagctaca gaagcacgc 
      ttaaagccg ccatgtggc gcagccacg gcgccaaag 
      accagcgac agagaatcg tgatcccgt tctttagtt 
      tgctggtca aggatttgt atttcctca accaggggt 
      gttccaata agcttccaa atggccaca taaattttg 
      aaaagttct ggcagttgg cgaaacagg tcacggagt 
      ttatggcct ggaagcagg tgacgtgtc cgttcgaga 
      aggacccca aagtcattt cagttctgc aacatggac 
      caattttaa acgagaacg cgctcgcgc tcgccagct 
      tcgactgcg agcccccgg agaacaacc aggagaagg 
      agaggtgca agacgctca aagccgaca gcactgcga 
      tgtaactct tttcgatgt acaaatggt ttgttttaa 
      ctgatctgt atataattg attgatgtc ttggatttt 
      atttttgtt ttgtacagg atatagttt tctttttta 
      aaaaacaga acacttgat aagtggtta ttttcctaa 
      tatgaagta tttaatgtg atttctagc tttttatcg 
      agtaattta gttggaaac tggcacaac ttttagagt 
      accttcacc cacacggat tccggtgtt aaatgctga 
      tattaattg agtacctaa ttgccattt ttatattaa 
      gtaccgctt tagctttta ttccttgag cgaagtttt 
      gatgtattt ttcataata aatcacgaa aactaaaaa 
      atattcaca cacttacat ttttgaaaa tttgtacct 
      atttgtaat gttttatac ctcagtgtt tttacataa 
      tgtttgtaa tgatgtact taatatact tgaccaatg 
      tattaacag tatttaatc taaattagt attcgtctt 
      gtcaggaac tcacactca atcacattt gttataaat 
      acgccatgc aatttttca ttgtaaatg aatgtgatt 
      atcataaat gggtaaata tttattgga tgtaacaga 
      tgtgattac tctattgat tgcaatgta gagccgaac 
      tcgctacat atgctttgc ctcacgctg cagatagga 
      tattaaaca attcaaaaa gacttaaat ctatcagta 
      ttctagtca ctaggtaag aaatgcatc gctaattat 
      tacactata tgttgtatt gtagttatt tataaaata 
      tataaatca tttgtatcg tacgcattg ttttatgta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY