Status
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Gene information
General information
TC003408 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 4464086..4466253
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003408
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00566

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003408 RA
      MLKSKLMSV MDLHTKQRQ FSGQLYKYT 
      NVMKGWQYR YFTVDANAG LLHYYLCEG 
      EKPDGSVPR GSVHLAGAV ICPSDEDSK 
      TFTVNCASG NMLKLRAAD ARARQEWVN 
      GLRAVAESH TKAISANSP PLPPREHLA 
      VLDAMGCVR AQLAQTEQA DLALCRTIE 
      SAGSKFFVD PNLLMLKAT SAASLHCLN 
      QCMNILQRN QHAQQARTG FVIDDD
    • >TC003408 RB
      MDLHTKQRQ FSGQLYKYT NVMKGWQYR 
      YFTVDANAG LLHYYLCEG EKPDGSVPR 
      GSVHLAGAV ICPSDEDSK TFTVNCASG 
      NMLKLRAAD ARARQEWVN GLRAVAESH 
      TKAISANSP PLPPREHLA VLDAMGCVR 
      AQLAQTEQA DLALCRTIE SAGSKFFVD 
      PNLLMLKAT SAASLHCLN QCMNILQRN 
      QHAQQARTG FVIDDD
    CDS sequence
    • >TC003408 RA
      atgttaaag agcaaactc atgtcagtt atggacttg 
      cacaccaaa cagaggcaa ttctcaggt caactgtac 
      aaatacact aatgtaatg aagggctgg cagtaccgc 
      tactttacg gtggacgca aacgcgggg cttttgcac 
      tactacctg tgcgagggc gaaaagccc gacgggagc 
      gtcccccgg ggctcggtg cacctggca ggggctgtg 
      atttgcccc agtgacgaa gactccaaa accttcaca 
      gtgaactgt gcctcaggg aatatgctc aagctgagg 
      gctgccgac gccagggcg cggcaggaa tgggtcaac 
      ggcctcagg gctgtggcc gaatcacac accaaggca 
      attagtgcc aacagcccc cctttgccc cctagggag 
      cacctcgcg gtgttggac gccatgggt tgtgtgcga 
      gcccagtta gcacagacc gagcaagcg gatttagcc 
      ttgtgtcgg acgattgag tcagctggg agcaagttt 
      tttgttgat cctaattta cttatgttg aaagctact 
      tcagccgct tcgttgcat tgtttgaac cagtgtatg 
      aacatttta caaagaaat caacacgcg cagcaagcg 
      aggactggg tttgtaatt gatgacgat taa
    • >TC003408 RB
      atggacttg cacaccaaa cagaggcaa ttctcaggt 
      caactgtac aaatacact aatgtaatg aagggctgg 
      cagtaccgc tactttacg gtggacgca aacgcgggg 
      cttttgcac tactacctg tgcgagggc gaaaagccc 
      gacgggagc gtcccccgg ggctcggtg cacctggca 
      ggggctgtg atttgcccc agtgacgaa gactccaaa 
      accttcaca gtgaactgt gcctcaggg aatatgctc 
      aagctgagg gctgccgac gccagggcg cggcaggaa 
      tgggtcaac ggcctcagg gctgtggcc gaatcacac 
      accaaggca attagtgcc aacagcccc cctttgccc 
      cctagggag cacctcgcg gtgttggac gccatgggt 
      tgtgtgcga gcccagtta gcacagacc gagcaagcg 
      gatttagcc ttgtgtcgg acgattgag tcagctggg 
      agcaagttt tttgttgat cctaattta cttatgttg 
      aaagctact tcagccgct tcgttgcat tgtttgaac 
      cagtgtatg aacatttta caaagaaat caacacgcg 
      cagcaagcg aggactggg tttgtaatt gatgacgat 
      taa
    mRNA sequence
    • >TC003408 RA
      gtaaaacaa caaatccat ggcttgtaa aatttcttt 
      tcggcacaa aaagccata actaggtca catttaaaa 
      taaaatata cataaggag gcaacattt tgcaagtta 
      ccaactagt tatccaatt tacataggc cacaaattc 
      ttttttgtt ttgatttag gtttttggg tgcttccct 
      tctcaagtt cagagtcga tgcggacga tcccatgtt 
      ttctatcat ttcagtgtt acctgactt acctgtatt 
      actcagctt aacatttcc cttaattct accaattca 
      ctgaaaatt taaagtcac gtaataact tcaagtcca 
      tattttaca tatctcccc gaattgtgc tgtttttca 
      caaatttta cgcgaaaat tttccattt cgacttcgt 
      ttttgacat ttgaagttt ctaggttat agacattta 
      tgacggcca acaacaaat tggttgagt aaatgcagt 
      tacaaaaac cactcgatg gatgttaaa gagcaaact 
      catgtcagt tatggactt gcacaccaa acagaggca 
      attctcagg tcaactgta caaatacac taatgtaat 
      gaagggctg gcagtaccg ctactttac ggtggacgc 
      aaacgcggg gcttttgca ctactacct gtgcgaggg 
      cgaaaagcc cgacgggag cgtcccccg gggctcggt 
      gcacctggc aggggctgt gatttgccc cagtgacga 
      agactccaa aaccttcac agtgaactg tgcctcagg 
      gaatatgct caagctgag ggctgccga cgccagggc 
      gcggcagga atgggtcaa cggcctcag ggctgtggc 
      cgaatcaca caccaaggc aattagtgc caacagccc 
      ccctttgcc ccctaggga gcacctcgc ggtgttgga 
      cgccatggg ttgtgtgcg agcccagtt agcacagac 
      cgagcaagc ggatttagc cttgtgtcg gacgattga 
      gtcagctgg gagcaagtt ttttgttga tcctaattt 
      acttatgtt gaaagctac ttcagccgc ttcgttgca 
      ttgtttgaa ccagtgtat gaacatttt acaaagaaa 
      tcaacacgc gcagcaagc gaggactgg gtttgtaat 
      tgatgacga ttaatatag ttttagagt taggcggta 
      tttaaatat tttcagtgc ttgtgtaag ttgtattaa 
      gtaataatc gaattgaat gtgattgta acattgtga 
      taatgaaat agtgttaac gtataagag ggaattcaa 
      aattgaaaa tacagtaga acttctata acccgaact 
      aactaccat tgcatttat aacaccaga ctagttact 
      aaaagtatt tatttacaa ccacttaat aaaatctga 
      cgaatacta tcttttgaa agtgggaaa taggtcacg 
      aaatcctat ggtcagtaa tataatttc aactcttag 
      gcgggaata tctggtatt gccaccaat ggtgtctag 
      aaaattatt aacgaaaat ctgctatta aatacttag 
      gcaattttt taataataa taaataata aaaaataca 
      caataatta ataaaaaat attaattaa tcgaaaatt 
      ttttgattt gtagcgtca gtctaaaga aatcagtca 
      ataaatcct aaaaataaa aaatttggt gtataattg 
      gttgcctac ccgcgagat gatttaata aaaactttt 
      tattgttta atattaaaa cacctattc atctgaaac 
      aaaacgaaa taatataga atataactt gcaaaaagt 
      catcttatt tcgtaacag agccaaaat tgagtgaaa 
      taagaaaga aacatgtta ccttatctg aaaaatgtt 
      ttcacttaa aaatgctgt aattttcca tgcaagtta 
      attaaaaaa acaatttct gaagggttg cattggcaa 
      agaattttt tatacttgt gacacaatt aaaaaaagg 
      gacactaa
    • >TC003408 RB
      gtaaaacaa caaatccat ggcttgtaa aatttcttt 
      tcggcacaa aaagccata actaggtca catttaaaa 
      taaaatata cataaggag gcaacattt tgcaagtta 
      ccaactagt tatccaatt tacataggc cacaaattc 
      ttttttgtt ttgatttag gtttttggg tgcttccct 
      tctcaagtt cagagtcga tgcggacga tcccatgtt 
      ttctatcat ttcagtgtt acctgactt acctgtatt 
      actcagctt aacatttcc cttaattct accaattca 
      ctgaaaatt taaagtcac gtaataact tcaagtcca 
      tattttaca tatctcccc gaattgtgc tgtttttca 
      caaatttta cgcgaaaat tttccattt cgacttcgt 
      ttttgacat ttgaagttt ctaggttat agacattta 
      tgacggcca acaacaaat tggttgagt cagttatgg 
      acttgcaca ccaaacaga ggcaattct caggtcaac 
      tgtacaaat acactaatg taatgaagg gctggcagt 
      accgctact ttacggtgg acgcaaacg cggggcttt 
      tgcactact acctgtgcg agggcgaaa agcccgacg 
      ggagcgtcc cccggggct cggtgcacc tggcagggg 
      ctgtgattt gccccagtg acgaagact ccaaaacct 
      tcacagtga actgtgcct cagggaata tgctcaagc 
      tgagggctg ccgacgcca gggcgcggc aggaatggg 
      tcaacggcc tcagggctg tggccgaat cacacacca 
      aggcaatta gtgccaaca gcccccctt tgcccccta 
      gggagcacc tcgcggtgt tggacgcca tgggttgtg 
      tgcgagccc agttagcac agaccgagc aagcggatt 
      tagccttgt gtcggacga ttgagtcag ctgggagca 
      agttttttg ttgatccta atttactta tgttgaaag 
      ctacttcag ccgcttcgt tgcattgtt tgaaccagt 
      gtatgaaca ttttacaaa gaaatcaac acgcgcagc 
      aagcgagga ctgggtttg taattgatg acgattaat 
      atagtttta gagttaggc ggtatttaa atattttca 
      gtgcttgtg taagttgta ttaagtaat aatcgaatt 
      gaatgtgat tgtaacatt gtgataatg aaatagtgt 
      taacgtata agagggaat tcaaaattg aaaatacag 
      tagaacttc tataacccg aactaacta ccattgcat 
      ttataacac cagactagt tactaaaag tatttattt 
      acaaccact taataaaat ctgacgaat actatcttt 
      tgaaagtgg gaaataggt cacgaaatc ctatggtca 
      gtaatataa tttcaactc ttaggcggg aatatctgg 
      tattgccac caatggtgt ctagaaaat tattaacga 
      aaatctgct attaaatac ttaggcaat tttttaata 
      ataataaat aataaaaaa tacacaata attaataaa 
      aaatattaa ttaatcgaa aattttttg atttgtagc 
      gtcagtcta aagaaatca gtcaataaa tcctaaaaa 
      taaaaaatt tggtgtata attggttgc ctacccgcg 
      agatgattt aataaaaac tttttattg tttaatatt 
      aaaacacct attcatctg aaacaaaac gaaataata 
      tagaatata acttgcaaa aagtcatct tatttcgta 
      acagagcca aaattgagt gaaataaga aagaaacat 
      gttacctta tctgaaaaa tgttttcac ttaaaaatg 
      ctgtaattt tccatgcaa gttaattaa aaaaacaat 
      ttctgaagg gttgcattg gcaaagaat tttttatac 
      ttgtgacac aattaaaaa aagggacac taa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY