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Gene information
General information
TC003402 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 4308792..4316316
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003402
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00565

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    larva appendages irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larva appendages sometimes fused - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    thorax partially fused - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003402 RA
      MAKDDFQEN GLEETVPLN TEYVKNKLK IKKTKDGKK 
      EDKKEEKKI SYFQLFRYT TLQDKLCIA LGTLCAVIC 
      GCIQPYVMI LFGDVTEVI IQFAETLKS NNSEINRTQ 
      AVDDLFRGV TDFAIYSSS SGIVMIITT YLAGILFSS 
      SALRQIFHI RKLILQKTL NMDISWYDL NKTGDFATT 
      FTENLSKLE EGIGEKVGI FLYFETIFV TGIVMGLVL 
      GWELALICL ISLPVSFAV AFLISWLST KFSKQELEA 
      YANAGAIAE EVLSSVRTV VAFDGQGKE FERYEKHLQ 
      AAKKNNIRK NLFTGVSNA VMWFFVFAS YALSFWYGV 
      GLILKEKEL PYEERVYTP GNMVSVFFC TLMASWNFG 
      TGAPYFEIF GTACGAAAK VFEILDTKP DINLSKTKG 
      LKPKNLKGD IVFKDVSFH YPSRPDVKI LQNFSIEIK 
      AGQTVALVG SSGCGKSTC IQLIQRFYD AVTGTVKID 
      DNNIKDLNL TWLRSKIGV VGQEPALFG ATIAENIKF 
      GNVTATQSD VERAAKKAN AHNFIQKLP RGYNTVVGE 
      RGAQLSGGQ KQRIAIARA LIREPKILL LDEATSALD 
      TTSEAEVQA ALDAVSGEC TTIIVAHRL STIRNANRI 
      VVVSHGSVI EEGTHSELM AKKGAYFDL VQSQGLVET 
      EETTTEEKQ KQNGVVDTK PNQTEVTEI ISTENLNDA 
      QAENKGSPI LQILKMNKP EWFHIFTGC VTAVINGSA 
      FPIYGLVFG DIIGVLADP RDSYVREQS NIFSLYFVI 
      IGIVTAVAT FLQIYYFAV AGEKLTKRL RAKMFRAML 
      NQEMAWFDR KENGVGALC AKLSGEAAS VQGAGGIRI 
      GTVLNSLAT FIISNIIAL YFEWRLALV LISFSPIIL 
      LSVFFEQKF TQGDSQVNQ KYLENSAKI AVEAIGNIR 
      TIASLGCEE VFHGYYVKE LTPYVANVK KQMHFRSAV 
      LGVARSVML FAYAVGMGY GAKLMVDSD VDYGTVFIV 
      SETVIVGSW SIGNAFSFS PNFQKGLSA ADRIFSLLK 
      RVPEVKNSL EPVYLNDVR GNIEYSNIY FSYPTRSSV 
      SVLNGLNLN VLQGKTVAL VGASGCGKS TIIQLLERF 
      YDPVSGEVS LDGESVKTV DIQNLRSHL GIVSQEPNL 
      FDRTIAENI AYGANDRTV GMNEIVEAA KSANIHTFI 
      SSLPGGYET SLGSKGAQL SGGQKQRVA IARALIRNP 
      KILLLDEAT SALDNESEK VVQEALDNA KKNRTCITI 
      AHRLTTIQD ADLICVLNE GVVAEMGKH NELLDKKGL 
      YYDFYKLQT GQK
    CDS sequence
    • >TC003402 RA
      atggcaaaa gatgatttt caagaaaac ggattggag 
      gaaacagta ccactaaat accgaatat gtgaaaaat 
      aaactgaaa atcaaaaag acaaaggat gggaaaaag 
      gaggacaaa aaggaggag aaaaaaatc tcatatttt 
      caactgttt cgttacacc acattgcaa gacaaatta 
      tgcatagct ttaggcacc ctgtgtgca gtaatatgc 
      ggctgcatc caaccttac gtaatgata ctttttgga 
      gatgttacc gaagtcatt atccaattc gccgagact 
      ttgaagtca aacaattcg gaaattaac agaacacaa 
      gctgtggac gacttattt aggggcgta acggacttt 
      gcgatttat tcctcaagc tcagggatt gtgatgata 
      attacgact tacttggcc gggatactt ttcagttca 
      agtgcctta agacagatc ttccatatt aggaaatta 
      attctgcaa aagacgtta aacatggac atttcctgg 
      tacgacctt aacaagacg ggagacttt gcgaccact 
      ttcacagaa aacttgtcg aaacttgag gaaggaata 
      ggcgaaaaa gttggcatt tttttgtac tttgagacg 
      atttttgtc actgggatt gtgatgggg cttgtgctg 
      ggctgggag ctggcccta atttgcctc atttcactg 
      ccagtgtcc ttcgctgtc gcgttctta atctcatgg 
      ttatccaca aaattctcg aaacaagaa ctcgaagcc 
      tacgctaac gccggtgcc atcgctgag gaagttcta 
      agctcggtc cggacagtt gtagctttc gacggccaa 
      ggaaaggaa ttcgaacgc tacgagaag cacctacaa 
      gctgccaag aaaaacaac atccgcaaa aacctattc 
      acgggcgta agtaacgcc gttatgtgg tttttcgtg 
      ttcgcctcc tacgcccta tcattctgg tacggcgtt 
      gggttgatt ttgaaagag aaggaactg ccctacgaa 
      gagcgagtt tacactcct ggaaatatg gtctccgtg 
      tttttttgt acgttaatg gcgtcctgg aatttcggg 
      actggcgcc ccgtatttc gaaattttc gggacggct 
      tgtggcgcc gccgccaaa gtgttcgag attttggac 
      acaaaaccc gacataaac ctctccaaa accaaaggc 
      ttgaaaccg aaaaacctg aaaggcgat atcgttttc 
      aaggacgtt tccttccat tatccttcc aggccggac 
      gtcaagatt ttgcaaaat ttcagtatt gaaatcaaa 
      gccggtcaa accgtagct ctggtcgga agttcgggt 
      tgcggaaaa tcaacgtgt attcaactg atccaaaga 
      ttttacgac gctgtgact ggcactgtc aaaattgac 
      gacaacaat atcaaagac ttaaattta acatggttg 
      aggagtaaa atcggggtt gtaggccag gaaccggca 
      cttttcggg gccacaatc gccgagaac ataaaattc 
      gggaacgtt acagccact caaagtgat gtcgaacgc 
      gctgccaaa aaagccaac gcccataat ttcatccaa 
      aaattgcca cgcggttac aataccgtc gttggcgaa 
      agaggggcc caactttcc gggggccaa aagcaaaga 
      attgcgata gctagggcc ttaatacgg gaacctaaa 
      atactgtta cttgatgaa gccacttcg gcccttgat 
      accacaagt gaggcggag gttcaagcg gccctggac 
      gctgtcagt ggcgagtgc accaccatt attgtagct 
      cacagatta tcaacaata aggaacgct aaccgaatt 
      gtggtcgtt tctcacgga tcggtgatc gaggaaggg 
      acacacagt gagctaatg gcgaaaaag ggggcttac 
      ttcgactta gtacagtcg caagggttg gtcgaaact 
      gaagaaaca accacagaa gaaaaacaa aaacagaac 
      ggtgtggtc gacactaaa cctaatcaa acggaagtt 
      actgaaatt atttccact gaaaatctg aacgacgct 
      caagcagag aataaagga agtccgata ctgcaaatt 
      ttaaaaatg aataaaccc gagtggttc cacattttc 
      acagggtgt gtaactgcg gttataaac ggttcagct 
      ttccccatt tatgggcta gttttcggt gatataatc 
      ggtgtgcta gccgaccct agagattct tatgttcgc 
      gagcagagt aatatcttt tctctgtac tttgtgatt 
      attgggatt gttacagct gtcgctacg tttttgcag 
      atttattat tttgccgtc gctggcgag aaactgacg 
      aaacgtctg cgcgcgaaa atgttccgc gcaatgtta 
      aaccaggaa atggcttgg tttgaccga aaggaaaac 
      ggggttggg gctctttgt gcaaaattg tcaggggag 
      gcggctagt gtccaaggg gctggtgga atccgaatt 
      ggaacagta cttaactcc ctggccacg tttataata 
      tctaacata atagcactt tatttcgaa tggagatta 
      gccttggtt ttgatatct ttttcgccc attatatta 
      ctgtcggtt ttctttgag caaaaattc acacagggc 
      gactctcaa gtgaatcag aaatatttg gaaaactcg 
      gcaaagatc gcagtggaa gctattggg aacattcga 
      acgattgcg tccttagga tgcgaggaa gtttttcac 
      ggctactat gtcaaagag ttgactcca tacgtggcc 
      aatgttaag aagcaaatg cactttaga tcggctgtt 
      ctgggtgtt gccagaagc gtaatgctt tttgcctac 
      gctgtcggg atgggatat ggcgccaaa ttaatggtg 
      gattctgat gtagactac ggaacagtc ttcatagtt 
      tccgaaact gtaatagtc ggctcatgg tcgatcggt 
      aacgcattt tcgttctcc cccaacttc cagaaggga 
      ttatccgcc gctgaccga atattctcg cttttgaag 
      cgcgtgcct gaagtgaaa aactcactc gaacctgtc 
      tatttaaac gacgtccga gggaacatc gaatactca 
      aacatttat ttctcatac cccactcgg tcctcagtc 
      tcggtacta aacggcctg aacttgaac gtgcttcaa 
      ggcaaaacc gtggctttg gtgggtgcg agtggttgt 
      ggcaaatca accatcatc caactgttg gaaaggttc 
      tacgatccg gtctccggc gaggtttcc ctcgacgga 
      gaatcggtc aaaactgtc gacattcaa aacttgcgg 
      tcacacctt ggcattgtg tcccaagaa ccgaacctt 
      ttcgacaga acaatagcc gagaatatt gcctatggg 
      gccaacgat cgcacagtt ggcatgaac gaaatcgtc 
      gaagcggcc aaatcggca aacatccac actttcata 
      tcgtctcta ccagggggt tatgaaact agtttgggt 
      agcaaaggg gcgcagttg tctgggggc caaaaacag 
      cgcgttgct attgctagg gctttgatt cggaatccg 
      aagattcta ttgctggat gaagcaact tcagcactc 
      gataacgag agcgaaaaa gtcgtgcaa gaagctcta 
      gataatgca aagaaaaac aggacttgt atcacaata 
      gcgcacaga ttgaccaca atccaagac gccgatctt 
      atttgtgtg ttaaacgag ggagttgtt gcagaaatg 
      ggaaagcac aatgagtta ctggacaaa aaggggctc 
      tattacgac ttctataaa ttacaaact gggcaaaag 
      tag
    mRNA sequence
    • >TC003402 RA
      cagcaataa atttacaaa tacaaattt atttaaagt 
      caagttgaa tggtactgt atccacaga ttggtgttc 
      cattgtcga attgattcc ggtttaaag cgaaagtga 
      cactgtaac atctccaac aaatatggc aaaagatga 
      ttttcaaga aaacggatt ggaggaaac agtaccact 
      aaataccga atatgtgaa aaataaact gaaaatcaa 
      aaagacaaa ggatgggaa aaaggagga caaaaagga 
      ggagaaaaa aatctcata ttttcaact gtttcgtta 
      caccacatt gcaagacaa attatgcat agctttagg 
      caccctgtg tgcagtaat atgcggctg catccaacc 
      ttacgtaat gatactttt tggagatgt taccgaagt 
      cattatcca attcgccga gactttgaa gtcaaacaa 
      ttcggaaat taacagaac acaagctgt ggacgactt 
      atttagggg cgtaacgga ctttgcgat ttattcctc 
      aagctcagg gattgtgat gataattac gacttactt 
      ggccgggat acttttcag ttcaagtgc cttaagaca 
      gatcttcca tattaggaa attaattct gcaaaagac 
      gttaaacat ggacatttc ctggtacga ccttaacaa 
      gacgggaga ctttgcgac cactttcac agaaaactt 
      gtcgaaact tgaggaagg aataggcga aaaagttgg 
      cattttttt gtactttga gacgatttt tgtcactgg 
      gattgtgat ggggcttgt gctgggctg ggagctggc 
      cctaatttg cctcatttc actgccagt gtccttcgc 
      tgtcgcgtt cttaatctc atggttatc cacaaaatt 
      ctcgaaaca agaactcga agcctacgc taacgccgg 
      tgccatcgc tgaggaagt tctaagctc ggtccggac 
      agttgtagc tttcgacgg ccaaggaaa ggaattcga 
      acgctacga gaagcacct acaagctgc caagaaaaa 
      caacatccg caaaaacct attcacggg cgtaagtaa 
      cgccgttat gtggttttt cgtgttcgc ctcctacgc 
      cctatcatt ctggtacgg cgttgggtt gattttgaa 
      agagaagga actgcccta cgaagagcg agtttacac 
      tcctggaaa tatggtctc cgtgttttt ttgtacgtt 
      aatggcgtc ctggaattt cgggactgg cgccccgta 
      tttcgaaat tttcgggac ggcttgtgg cgccgccgc 
      caaagtgtt cgagatttt ggacacaaa acccgacat 
      aaacctctc caaaaccaa aggcttgaa accgaaaaa 
      cctgaaagg cgatatcgt tttcaagga cgtttcctt 
      ccattatcc ttccaggcc ggacgtcaa gattttgca 
      aaatttcag tattgaaat caaagccgg tcaaaccgt 
      agctctggt cggaagttc gggttgcgg aaaatcaac 
      gtgtattca actgatcca aagatttta cgacgctgt 
      gactggcac tgtcaaaat tgacgacaa caatatcaa 
      agacttaaa tttaacatg gttgaggag taaaatcgg 
      ggttgtagg ccaggaacc ggcactttt cggggccac 
      aatcgccga gaacataaa attcgggaa cgttacagc 
      cactcaaag tgatgtcga acgcgctgc caaaaaagc 
      caacgccca taatttcat ccaaaaatt gccacgcgg 
      ttacaatac cgtcgttgg cgaaagagg ggcccaact 
      ttccggggg ccaaaagca aagaattgc gatagctag 
      ggccttaat acgggaacc taaaatact gttacttga 
      tgaagccac ttcggccct tgataccac aagtgaggc 
      ggaggttca agcggccct ggacgctgt cagtggcga 
      gtgcaccac cattattgt agctcacag attatcaac 
      aataaggaa cgctaaccg aattgtggt cgtttctca 
      cggatcggt gatcgagga agggacaca cagtgagct 
      aatggcgaa aaagggggc ttacttcga cttagtaca 
      gtcgcaagg gttggtcga aactgaaga aacaaccac 
      agaagaaaa acaaaaaca gaacggtgt ggtcgacac 
      taaacctaa tcaaacgga agttactga aattatttc 
      cactgaaaa tctgaacga cgctcaagc agagaataa 
      aggaagtcc gatactgca aattttaaa aatgaataa 
      acccgagtg gttccacat tttcacagg gtgtgtaac 
      tgcggttat aaacggttc agctttccc catttatgg 
      gctagtttt cggtgatat aatcggtgt gctagccga 
      ccctagaga ttcttatgt tcgcgagca gagtaatat 
      cttttctct gtactttgt gattattgg gattgttac 
      agctgtcgc tacgttttt gcagattta ttattttgc 
      cgtcgctgg cgagaaact gacgaaacg tctgcgcgc 
      gaaaatgtt ccgcgcaat gttaaacca ggaaatggc 
      ttggtttga ccgaaagga aaacggggt tggggctct 
      ttgtgcaaa attgtcagg ggaggcggc tagtgtcca 
      aggggctgg tggaatccg aattggaac agtacttaa 
      ctccctggc cacgtttat aatatctaa cataatagc 
      actttattt cgaatggag attagcctt ggttttgat 
      atctttttc gcccattat attactgtc ggttttctt 
      tgagcaaaa attcacaca gggcgactc tcaagtgaa 
      tcagaaata tttggaaaa ctcggcaaa gatcgcagt 
      ggaagctat tgggaacat tcgaacgat tgcgtcctt 
      aggatgcga ggaagtttt tcacggcta ctatgtcaa 
      agagttgac tccatacgt ggccaatgt taagaagca 
      aatgcactt tagatcggc tgttctggg tgttgccag 
      aagcgtaat gctttttgc ctacgctgt cgggatggg 
      atatggcgc caaattaat ggtggattc tgatgtaga 
      ctacggaac agtcttcat agtttccga aactgtaat 
      agtcggctc atggtcgat cggtaacgc attttcgtt 
      ctcccccaa cttccagaa gggattatc cgccgctga 
      ccgaatatt ctcgctttt gaagcgcgt gcctgaagt 
      gaaaaactc actcgaacc tgtctattt aaacgacgt 
      ccgagggaa catcgaata ctcaaacat ttatttctc 
      ataccccac tcggtcctc agtctcggt actaaacgg 
      cctgaactt gaacgtgct tcaaggcaa aaccgtggc 
      tttggtggg tgcgagtgg ttgtggcaa atcaaccat 
      catccaact gttggaaag gttctacga tccggtctc 
      cggcgaggt ttccctcga cggagaatc ggtcaaaac 
      tgtcgacat tcaaaactt gcggtcaca ccttggcat 
      tgtgtccca agaaccgaa ccttttcga cagaacaat 
      agccgagaa tattgccta tggggccaa cgatcgcac 
      agttggcat gaacgaaat cgtcgaagc ggccaaatc 
      ggcaaacat ccacacttt catatcgtc tctaccagg 
      gggttatga aactagttt gggtagcaa aggggcgca 
      gttgtctgg gggccaaaa acagcgcgt tgctattgc 
      tagggcttt gattcggaa tccgaagat tctattgct 
      ggatgaagc aacttcagc actcgataa cgagagcga 
      aaaagtcgt gcaagaagc tctagataa tgcaaagaa 
      aaacaggac ttgtatcac aatagcgca cagattgac 
      cacaatcca agacgccga tcttatttg tgtgttaaa 
      cgagggagt tgttgcaga aatgggaaa gcacaatga 
      gttactgga caaaaaggg gctctatta cgacttcta 
      taaattaca aactgggca aaagtagtt aatttaggc 
      acaattgtt aatgtagtt aagctacgt gtgttaaga 
      aacgggctt tcagattgg caaaatctc agtttgata 
      aatgaaact att
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY