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Gene information
General information
TC003392 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid14: 2912304..2914199
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003392
    iBeetle screen iB_00563
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 7 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    remarks: animals lost,
    Pupal morphology
    remarks: animals lost,
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003392 RA
      MNMRDKEKY IENFHFPFC DESEKYEKI AKIGQGTFG 
      EVFKARDKS NPKKFVAMK KVLMDNEKE GFPITALRE 
      IRILQLLKH ENVVNLIEI CQTKAAYRN RYKSTFYLV 
      FDFCEHDLA GLLSNVHVK FSLGEIKKV VQQLFNGLY 
      YIHSNKILH RDMKAANVL ITKNGVLKL ADFGLARAF 
      STNKNGLPN RFTNRVVTL WYRPPELLL GERNYGPPV 
      DLWGAGCIM AEMWTRSPI MQGNSEQQQ LTLISQLCG 
      TICPQVWPG VENLELYKK MELPVQKRK VKERLKHYM 
      KDPYACDLL DKLLVLDPA KRADADTAL NHDFFWTDP 
      MPCDLSKML ATHTQSMFE YLAPPRRPT QMRHHSLPR 
      PTTSAVQDS GYQDRVF
    CDS sequence
    • >TC003392 RA
      atgaatatg cgagataaa gagaaatac attgaaaat 
      ttccatttc ccgttctgt gatgaatcg gaaaaatac 
      gagaaaatt gcgaaaatt ggccaagga acgttcggt 
      gaagtgttc aaggcgagg gacaaaagt aatcctaag 
      aaattcgta gcaatgaaa aaagtgtta atggacaat 
      gaaaaagag ggctttcca atcacagct ttgcgcgaa 
      atacgaatt ttgcaacta ttgaaacac gaaaacgtt 
      gttaatctt atagaaata tgccaaact aaggctgca 
      taccgcaac agatacaag tcaacgttt tatttagtg 
      ttcgatttt tgtgaacac gacttggcc gggttgttg 
      tcaaacgtc cacgttaag tttagtcta ggagagata 
      aaaaaagta gtgcaacaa ttatttaac ggtctgtat 
      tacatacac agcaacaaa attcttcat agagatatg 
      aaagccgcc aatgtgtta attacaaag aatggtgtt 
      ttaaaattg gccgatttt ggcctagct cgtgcattt 
      agtaccaat aagaatgga ctgccaaat agatttaca 
      aatcgtgtc gttacgtta tggtatagg cctccagaa 
      ctgttgtta ggtgaaaga aactacggc cccccagtt 
      gatttgtgg ggggctggc tgtattatg gccgaaatg 
      tggacaagg tcccccata atgcagggc aacagcgag 
      caacaacag ctcactctg atttctcaa ctttgtggc 
      accatctgc ccccaggtc tggccagga gttgagaat 
      ctggagttg tacaagaag atggaactc cccgtccaa 
      aagcgaaaa gtgaaagag aggctgaag cactacatg 
      aaagacccg tacgcttgt gatttactc gataagctg 
      ttggtcctc gaccccgcc aaacgggcc gatgctgat 
      acggccctc aaccatgac tttttctgg actgaccca 
      atgccatgc gatttgagc aaaatgtta gccactcac 
      actcaaagc atgtttgaa tatttggct ccgccgaga 
      agaccgaca caaatgagg catcattcg ttgccaaga 
      ccgacaact tccgctgtg caagacagc ggataccaa 
      gatagggtt ttttag
    mRNA sequence
    • >TC003392 RA
      ctattgtca tatgtcatt cctggaggt tatgttttg 
      attgtgtgt gtttttagt gcaattgtg attcagaaa 
      aaagcagcg gaaatgcat ttaagtact ttaaatgtg 
      gtgttttaa accttcaca cgtttcttc ttaattgac 
      gaaaaactg tgctaaata ttgccttgt ctaaagctt 
      gtgcaacgt tagacaaga ggcgatttt gcaatcatg 
      aatatgcga gataaagag aaatacatt gaaaatttc 
      catttcccg ttctgtgat gaatcggaa aaatacgag 
      aaaattgcg aaaattggc caaggaacg ttcggtgaa 
      gtgttcaag gcgagggac aaaagtaat cctaagaaa 
      ttcgtagca atgaaaaaa gtgttaatg gacaatgaa 
      aaagagggc tttccaatc acagctttg cgcgaaata 
      cgaattttg caactattg aaacacgaa aacgttgtt 
      aatcttata gaaatatgc caaactaag gctgcatac 
      cgcaacaga tacaagtca acgttttat ttagtgttc 
      gatttttgt gaacacgac ttggccggg ttgttgtca 
      aacgtccac gttaagttt agtctagga gagataaaa 
      aaagtagtg caacaatta tttaacggt ctgtattac 
      atacacagc aacaaaatt cttcataga gatatgaaa 
      gccgccaat gtgttaatt acaaagaat ggtgtttta 
      aaattggcc gattttggc ctagctcgt gcatttagt 
      accaataag aatggactg ccaaataga tttacaaat 
      cgtgtcgtt acgttatgg tataggcct ccagaactg 
      ttgttaggt gaaagaaac tacggcccc ccagttgat 
      ttgtggggg gctggctgt attatggcc gaaatgtgg 
      acaaggtcc cccataatg cagggcaac agcgagcaa 
      caacagctc actctgatt tctcaactt tgtggcacc 
      atctgcccc caggtctgg ccaggagtt gagaatctg 
      gagttgtac aagaagatg gaactcccc gtccaaaag 
      cgaaaagtg aaagagagg ctgaagcac tacatgaaa 
      gacccgtac gcttgtgat ttactcgat aagctgttg 
      gtcctcgac cccgccaaa cgggccgat gctgatacg 
      gccctcaac catgacttt ttctggact gacccaatg 
      ccatgcgat ttgagcaaa atgttagcc actcacact 
      caaagcatg tttgaatat ttggctccg ccgagaaga 
      ccgacacaa atgaggcat cattcgttg ccaagaccg 
      acaacttcc gctgtgcaa gacagcgga taccaagat 
      agggttttt tagttgtaa gaaacacgg acattgtgg 
      ttattttca agtaatgtg gaaattgtt tctaaagtg 
      gaaacataa ctgaaggtt tttcgggaa ccctaaaat 
      ttgatgtta ctttttatt atctttttg taacgttta 
      atatgatgt tacttagtt aacatagcg aataaactg 
      tgtaaaaac gc