Status
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Gene information
General information
TC003375 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 4146909..4149071
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003375
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00562

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    remarks: later than schedule, hours 24
    Pupal morphology
    remarks: later than schedule, hours 24
    Adult morphology
    remarks: later than schedule, hours 24
    Fertility
    remarks: later than schedule, hours 24
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003375 RA
      MVKKLLVAL SLLINVASL LGAPEIILP NGPIRGKEA 
      TTIKNTHYY AFEKIPYAT PPVGQLRFK APIPPANWD 
      EPLDTTNMD VECYQQQFN KESESEDCL YLNVFTPQL 
      PDDKNDPIP VMVYIHGGG FVGGSGRHT YGDRIVDQN 
      LVFVAINYR LGPFGFLST QDEVIPGNN GLKDQHLAL 
      KWVHDNINL FGGDPDKIT IFGQSAGAA SCAYQLLNQ 
      QNEGLFRAA ILQSGTFLS PWSFQRRAR EIAFDTAAF 
      INDTFKTNR DSQTLFEFL QSVDAKALD RASEQYHDT 
      EENPNDTEI LHGFFWTPV VEVKNPDAF LTKKMYGLL 
      QAGNVIRVP TLLGITSEE NLAFNTDAA TLKNVMGIY 
      DENLGWLVP HDMAVTDET NRTNIGSKI REIYTGGEP 
      LAEHLGDGV RYSSDTSFT RSVIKHAEI YSALADTFF 
      YQFSYDGEI PGFDVHYDG AESVAHSED YAYIFCGGT 
      DCDVSKYPQ SDQTTVDRI ISIWTNFAK YQNPTPEPS 
      ELLQNITWP LVSTENGDF LYVDINENL EIRNHPKEA 
      TYQKWVELY DSLGYSDFD TY
    CDS sequence
    • >TC003375 RA
      atggttaag aagttattg gtcgcgcta agtttatta 
      ataaacgtt gcttctctg cttggagct cccgaaatt 
      atactacca aacggtcca attagaggc aaagaagca 
      accacaatt aaaaacaca cattactat gctttcgaa 
      aaaattccg tatgcaact cctccagtt ggacagtta 
      cgtttcaag gcgcccata ccaccagca aactgggat 
      gaaccctta gataccact aacatggac gtagagtgt 
      taccaacaa cagtttaac aaagagtct gagtcggaa 
      gactgtctt tatctcaat gttttcacc cctcagctt 
      cctgatgat aaaaacgat cccataccg gttatggtg 
      tacatacat ggtggtggt tttgttggt ggttctggt 
      agacacacc tatggtgat cgtatagtt gaccaaaat 
      cttgtattt gttgctatt aattacaga ttagggcct 
      tttggtttt ctgtcaaca caagacgaa gtaatcccg 
      ggtaacaac ggtctaaaa gatcagcac ttagcctta 
      aaatgggta cacgacaat atcaacttg ttcggtggt 
      gatcctgat aagataact atttttggc caaagtgcc 
      ggtgcggcc tcttgtgct tatcagtta ttaaatcaa 
      caaaatgaa ggtttgttc cgagccgct attttacaa 
      agtgggacg tttttgagt ccttggtct ttccaaaga 
      agggccagg gagattgct tttgacacg gcggctttt 
      ataaacgac acgtttaaa accaatcgt gactcacaa 
      actttgttc gaatttttg caaagtgtg gacgcaaaa 
      gcactggat cgagcgtcc gagcaatat catgataca 
      gaggaaaat ccaaatgat acggaaatc ttgcatggg 
      tttttttgg accccagtt gtggaagtc aaaaatccg 
      gatgctttt ctcacgaaa aaaatgtat ggtttgctt 
      caagccgga aatgttatt cgagttcct accttgctt 
      ggtattact tcggaagaa aatcttgct tttaatacg 
      gacgcagct actttgaaa aatgtgatg ggcatctat 
      gacgaaaat ttgggttgg ttagtgcca catgatatg 
      gcagtgacc gatgagaca aatcgtacc aacattggg 
      tcaaaaata cgggagatt tacacaggt ggtgaacct 
      ttagccgag catcttggg gacggtgtt cggtattct 
      agtgacact tcatttaca cgctctgtt atcaaacac 
      gctgaaatt tattcggca ttggccgat acgttcttt 
      tatcagttt tcctatgac ggagagatt cctggattc 
      gatgtccat tatgatggt gctgagagt gttgctcat 
      agtgaagat tatgcttac attttttgt gggggaaca 
      gactgcgat gttagtaaa tatccacaa tctgatcaa 
      accacagtt gatagaata atttcgatt tggaccaac 
      tttgctaaa taccaaaac ccaactcca gaaccatct 
      gaactgctg caaaatatt acgtggcct ctagtttcc 
      accgagaat ggtgacttt ttatacgtt gacataaac 
      gaaaatctt gaaataaga aatcatcca aaggaagct 
      acgtaccaa aagtgggtc gaactgtac gatagttta 
      gggtattca gatttcgac acatattag
    mRNA sequence
    • >TC003375 RA
      acctacaag atggttaag aagttattg gtcgcgcta 
      agtttatta ataaacgtt gcttctctg cttggagct 
      cccgaaatt atactacca aacggtcca attagaggc 
      aaagaagca accacaatt aaaaacaca cattactat 
      gctttcgaa aaaattccg tatgcaact cctccagtt 
      ggacagtta cgtttcaag gcgcccata ccaccagca 
      aactgggat gaaccctta gataccact aacatggac 
      gtagagtgt taccaacaa cagtttaac aaagagtct 
      gagtcggaa gactgtctt tatctcaat gttttcacc 
      cctcagctt cctgatgat aaaaacgat cccataccg 
      gttatggtg tacatacat ggtggtggt tttgttggt 
      ggttctggt agacacacc tatggtgat cgtatagtt 
      gaccaaaat cttgtattt gttgctatt aattacaga 
      ttagggcct tttggtttt ctgtcaaca caagacgaa 
      gtaatcccg ggtaacaac ggtctaaaa gatcagcac 
      ttagcctta aaatgggta cacgacaat atcaacttg 
      ttcggtggt gatcctgat aagataact atttttggc 
      caaagtgcc ggtgcggcc tcttgtgct tatcagtta 
      ttaaatcaa caaaatgaa ggtttgttc cgagccgct 
      attttacaa agtgggacg tttttgagt ccttggtct 
      ttccaaaga agggccagg gagattgct tttgacacg 
      gcggctttt ataaacgac acgtttaaa accaatcgt 
      gactcacaa actttgttc gaatttttg caaagtgtg 
      gacgcaaaa gcactggat cgagcgtcc gagcaatat 
      catgataca gaggaaaat ccaaatgat acggaaatc 
      ttgcatggg tttttttgg accccagtt gtggaagtc 
      aaaaatccg gatgctttt ctcacgaaa aaaatgtat 
      ggtttgctt caagccgga aatgttatt cgagttcct 
      accttgctt ggtattact tcggaagaa aatcttgct 
      tttaatacg gacgcagct actttgaaa aatgtgatg 
      ggcatctat gacgaaaat ttgggttgg ttagtgcca 
      catgatatg gcagtgacc gatgagaca aatcgtacc 
      aacattggg tcaaaaata cgggagatt tacacaggt 
      ggtgaacct ttagccgag catcttggg gacggtgtt 
      cggtattct agtgacact tcatttaca cgctctgtt 
      atcaaacac gctgaaatt tattcggca ttggccgat 
      acgttcttt tatcagttt tcctatgac ggagagatt 
      cctggattc gatgtccat tatgatggt gctgagagt 
      gttgctcat agtgaagat tatgcttac attttttgt 
      gggggaaca gactgcgat gttagtaaa tatccacaa 
      tctgatcaa accacagtt gatagaata atttcgatt 
      tggaccaac tttgctaaa taccaaaac ccaactcca 
      gaaccatct gaactgctg caaaatatt acgtggcct 
      ctagtttcc accgagaat ggtgacttt ttatacgtt 
      gacataaac gaaaatctt gaaataaga aatcatcca 
      aaggaagct acgtaccaa aagtgggtc gaactgtac 
      gatagttta gggtattca gatttcgac acatattag 
      aaaaaaaca gcacttgca aatggtggg aaaaattaa 
      ttgaaatgt aattaaaaa caagaaata aaatattat 
      acttcaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY