GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_00561 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
head capsule mostly not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
head appendages random not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
head irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
prothoracic leg sometimes not present - Penetrance (percentage of animals/eggs on slide): less than 30%
thorax irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
- >TC003368 RA
MILAIVIAL ISAPFIWWM LILSKYRSL SNVPGPKPS PVIGNSLHI GYRPQDLFN NIVKWAKEY GTVYKIFVA NDVRVLITN PELTELVLS SNVHITKSN AYDLLKPWL GIGLLISTG KKWKSRRKL ITPTFHFKI LDQFLDVFN SCGNVLVQR LSSQVGKDS FDVYPFINL CSLDIICET SMGVKIKAQ QGNNTEYVE AVRRFLDIF IIRTFSVWK GIDALFKFS SAYSVYKRL LKILHQFTI NIIVQRRGE KAQQKTQNV TSDDGIKRK VALLEMLLE SEDNNMLSN EDIREEIDT FMFEGHDTT TSGIAFAIL CLAENPKVQ EKLYEEVVA VIDNIENIT MQQLQEMKY LEMVLKEAQ RLYPSVPVI ERRLEVDCN IGGYDFPKD TFLSLFIYG MHHNEKYFP EPEKFDPNR YLPENQAKR HNYAYVPFS AGPRNCIGQ KFAMLEMKT TIAKIVKHF KILPVPDYK PDLGIAAIL KSYNGVCVR LQHRHGN
- >TC003368 RA
atgattttg gcaatagtt atagctttg ataagtgca cccttcatt tggtggatg ctcattttg tcgaaatat aggtcctta agcaacgtc cctggcccg aaaccttca cctgtcatt ggtaattct cttcatata gggtacagg ccccaagac ttattcaat aacattgtg aaatgggca aaagaatac ggcacagtg tacaaaata tttgtggcg aacgacgta cgagtcctt attacaaat cctgaactg actgaacta gtgctcagc tcaaacgtg cacattacc aagtcgaac gcttacgat ttgctcaag ccatggcta ggaatcggg ttgttaata agcacagga aaaaaatgg aagagtcgt cgaaagctg ataacgcca acgttccac tttaaaatc cttgaccaa ttcctggac gttttcaat tcatgtggg aacgttctt gtgcagaga ctgtccagt caagtgggc aaagattcc ttcgacgtg tatcccttt ataaacctc tgctccttg gatataata tgtgaaacc tcaatggga gttaaaatc aaagcacag cagggaaat aatacagaa tacgtggaa gccgttcgc cgattcctc gacattttt atcatccgg acattttcg gtttggaag ggaatcgat gccttattt aaattttca agtgcttat agcgtctac aaacgctta ttaaaaatt ttacaccaa ttcacaatc aacattatc gtccaacgt cgtggggaa aaagcgcag caaaaaacc caaaacgtg acgtccgat gacggcatc aaacgcaaa gttgccctc ctggaaatg ctgctagaa tccgaagac aacaacatg ctctcaaac gaagacatc agggaggag attgacacg ttcatgttc gaaggacac gacaccaca acctcgggg atcgccttc gccatcctc tgcctggcc gagaatccc aaagtgcag gaaaaactc tacgaagaa gtggtcgcg gtaatcgac aacatcgag aatattacg atgcaacag ctgcaagaa atgaaatat ctggaaatg gtgctgaaa gaagcccaa cggctgtac ccctctgtg ccggttatt gaacgccgg ctggaagtt gactgtaat ataggcggg tatgacttt cccaaagat acgtttctc tcgctgttc atctacgga atgcatcac aacgaaaaa tatttccct gaaccggag aaattcgat cctaataga tatctgccc gaaaaccag gcaaagagg cacaattat gcctatgtg ccgttcagt gccggccca cgtaattgc attggccaa aaattcgca atgcttgaa atgaaaaca accatcgcc aaaatcgtg aaacacttc aaaatactg ccagtgcct gattacaag ccagatttg ggaatagcc gctattttg aagtcttat aacggggtt tgtgtgcga ctccagcat aggcatggg aactaa
- >TC003368 RA
aaataaatg cctaaaatg caccaacct gaactagtc catgatgaa tgtcatcaa gtattgcgt gatcttgtg ataaagatc gagtagcaa gttttttgc gacgtcttt tgtgcattg tgagaaatc tcccagagc agcctagag aaggactag aacccattg catctaaaa gtacccagt cttctttgg aaaaacacc gcgtcaaag ttggaactt caactggtc ctgcaagga aaataaaac aaatattac acgtggact ctgaactga ttttgtgtc caatgccac agtaaagaa aacaagccg acttgcttc cgatgcact gcgcatcta ttgatagtg tctatcttt tactcgtaa atcaacaac tttttcata atttgtatt agtgcgcac gtgacctta tatataaac gaataaact gcaattgct tgtaatagt tgatcagtg gtgttttat gtcttcaat cacaaaact ctgtcatga ttttggcaa tagttatag ctttgataa gtgcaccct tcatttggt ggatgctca ttttgtcga aatataggt ccttaagca acgtccctg gcccgaaac cttcacctg tcattggta attctcttc atatagggt acaggcccc aagacttat tcaataaca ttgtgaaat gggcaaaag aatacggca cagtgtaca aaatatttg tggcgaacg acgtacgag tccttatta caaatcctg aactgactg aactagtgc tcagctcaa acgtgcaca ttaccaagt cgaacgctt acgatttgc tcaagccat ggctaggaa tcgggttgt taataagca caggaaaaa aatggaaga gtcgtcgaa agctgataa cgccaacgt tccacttta aaatccttg accaattcc tggacgttt tcaattcat gtgggaacg ttcttgtgc agagactgt ccagtcaag tgggcaaag attccttcg acgtgtatc cctttataa acctctgct ccttggata taatatgtg aaacctcaa tgggagtta aaatcaaag cacagcagg gaaataata cagaatacg tggaagccg ttcgccgat tcctcgaca tttttatca tccggacat tttcggttt ggaagggaa tcgatgcct tatttaaat tttcaagtg cttatagcg tctacaaac gcttattaa aaattttac accaattca caatcaaca ttatcgtcc aacgtcgtg gggaaaaag cgcagcaaa aaacccaaa acgtgacgt ccgatgacg gcatcaaac gcaaagttg ccctcctgg aaatgctgc tagaatccg aagacaaca acatgctct caaacgaag acatcaggg aggagattg acacgttca tgttcgaag gacacgaca ccacaacct cggggatcg ccttcgcca tcctctgcc tggccgaga atcccaaag tgcaggaaa aactctacg aagaagtgg tcgcggtaa tcgacaaca tcgagaata ttacgatgc aacagctgc aagaaatga aatatctgg aaatggtgc tgaaagaag cccaacggc tgtacccct ctgtgccgg ttattgaac gccggctgg aagttgact gtaatatag gcgggtatg actttccca aagatacgt ttctctcgc tgttcatct acggaatgc atcacaacg aaaaatatt tccctgaac cggagaaat tcgatccta atagatatc tgcccgaaa accaggcaa agaggcaca attatgcct atgtgccgt tcagtgccg gcccacgta attgcattg gccaaaaat tcgcaatgc ttgaaatga aaacaacca tcgccaaaa tcgtgaaac acttcaaaa tactgccag tgcctgatt acaagccag atttgggaa tagccgcta ttttgaagt cttataacg gggtttgtg tgcgactcc agcataggc atgggaact aaacagtaa taattgtaa taattatta ggttaagaa cacaaaagg agtgtgtca atgtcatta cgttcaata aatgttttt tacttga
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
