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Gene information
General information
TC003367 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 2691468..2694990
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003367
    iBeetle screen iB_00560

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 90.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003367 RA
      MVDDYDEIQ AGAIAAPQM AEMPEIKLF 
      GRWSCDDVQ VPDMSLQDY IAVKEKNAK 
      YLPHSAGRY AAKRFRKAQ CPIVERLTN 
      SLMMHGRNN GKKLMAVRI VKHAFEIIH 
      LLTGDNPLE VLVNAIINS GPREDSTRI 
      GRAGTVRRQ AVDVSPLRR VNQAIWLLC 
      TGAREAAFR NIKTIAECL ADELMNAAK 
      GSSNSYAIK KKDELERVA KSNR
    CDS sequence
    • >TC003367 RA
      atggtggac gattacgat gaaatccaa gcgggggct 
      atcgccgcg ccccaaatg gcggaaatg ccagaaatt 
      aaattgttc ggccggtgg agctgcgac gatgttcaa 
      gtcccagat atgtctttg caggactac attgccgta 
      aaagagaag aatgccaaa tatttacct cactcagca 
      ggtcgttat gcggcaaaa aggttccgc aaggcccag 
      tgccccatt gttgaaaga ttgaccaac tctttgatg 
      atgcatggg aggaacaac ggcaaaaaa ttgatggcc 
      gtccgtatt gtcaaacat gcgtttgaa atcatccac 
      cttctaact ggagacaac ccacttgaa gttttggtt 
      aatgccatt attaattct ggcccccgt gaagactca 
      acccgtatt ggaagggca ggtaccgtt aggaggcaa 
      gccgttgat gtctctccc ttgcgtagg gtgaaccaa 
      gctatttgg ttattgtgc acaggtgca agagaagcc 
      gcattccgt aacatcaaa acgattgcg gaatgtttg 
      gctgatgaa ctcatgaat gcggcaaag ggatcatcc 
      aattcgtat gctattaag aaaaaggat gagttggaa 
      cgtgtggct aagtctaat cgttaa
    mRNA sequence
    • >TC003367 RA
      ttgctcata atttaataa atattagac agaaattcc 
      aggaagaat ggtggacga ttacgatga aatccaagc 
      gggggctat cgccgcgcc ccaaatggc ggaaatgcc 
      agaaattaa attgttcgg ccggtggag ctgcgacga 
      tgttcaagt cccagatat gtctttgca ggactacat 
      tgccgtaaa agagaagaa tgccaaata tttacctca 
      ctcagcagg tcgttatgc ggcaaaaag gttccgcaa 
      ggcccagtg ccccattgt tgaaagatt gaccaactc 
      tttgatgat gcatgggag gaacaacgg caaaaaatt 
      gatggccgt ccgtattgt caaacatgc gtttgaaat 
      catccacct tctaactgg agacaaccc acttgaagt 
      tttggttaa tgccattat taattctgg cccccgtga 
      agactcaac ccgtattgg aagggcagg taccgttag 
      gaggcaagc cgttgatgt ctctccctt gcgtagggt 
      gaaccaagc tatttggtt attgtgcac aggtgcaag 
      agaagccgc attccgtaa catcaaaac gattgcgga 
      atgtttggc tgatgaact catgaatgc ggcaaaggg 
      atcatccaa ttcgtatgc tattaagaa aaaggatga 
      gttggaacg tgtggctaa gtctaatcg ttaattgta 
      ttcatgtcc ttttgttac attggggca aataaagaa 
      aacatttct aattcagtg gtttccttt aaacctggc 
      cctggggaa taaaaaagc ggtttaaaa acatgaatt 
      tatttatca accatttca caaacgatg agtactcat 
      gttgaactc agattttaa gcgtaacaa taggattct 
      tataaccct ttgtctgta tacctccct aatgtcatt 
      ctctaagca gtcttattg aaacacatc atatattaa 
      ccctgttag ttcaactgt atggtccta aagtgaaat 
      tatatagtt ttgataaca ctgaaatgt ttgtgtctg 
      gagtctatc ggttgtatg ttagggtac aatttaaca 
      cacacttat gcaacttcc agtaactat gtatgagtt 
      atgtgtgta tgaatttgt ggtcttatg cggatatta 
      taattgatc ccaagatga caccagaaa gtggatgtg 
      ttaaaggag agacagact attgttcgc tagaaaact 
      taattatga ttctctaaa agtacattc cgttacctc 
      aaagaacta tgtacctac gaatatttg cagagtcaa 
      cgtaaatcc cttaacaag tcatggaat tttgattat 
      tacgtctgc aacaaccta ctacgaaca tacagtata 
      cagcacatt ataaaatga accacattc aacagttat 
      tacagagta atgttgcaa gagggtacg gtatactaa 
      gtgaaacgt ttgcaaaga tcgcggcat attgtctaa 
      aaaagtggt aaagtgtga gaaagaaat gtacgcgaa 
      catattgtc gactagtga tctttgcaa atgtccgat 
      acattgtac aatatataa aaaagaaaa cacagagta 
      aacatcctg tcggggttt gcaacagtc gtttcatta 
      caaatataa tatatcaag aaagttgat tcaaagtta 
      tattcatat caaatcact aagattgca ccacattca 
      aaatctgta acaaactca acaacacta caactaaaa 
      tacttttca tgtaaggta cttagactt gtacataac 
      gaactaagt attaaatag atgaatatg cgcaaccgt 
      agtgcaaat aaattacta cgtctagtt tctagggaa 
      gaactacct atcgtcaca tacactgcg catgtaaaa 
      cataaaaat cctaacaat cattgtgac aactagaac 
      atttatgat tatttggct tgtttgatg attataaat 
      aatgagcta actagtgaa gatgtcagt cagtgaatg 
      ttggtcctt attgcacat tggacggtg atgtccaga 
      gccctattg gcgatagag gcaaccgtc aaaacactt 
      gaaaataaa atcacagtt agtttttgt ttgccgatc 
      attaaatac tatgttcgc ggagccgca aatcgctcc 
      tcatcaact tcacaatct caggttacc tgcaacttt 
      aaaattgga tcgcaaaaa catgtaatc acaatggac 
      tttttcaaa attctatcg tactaattc ttttttgaa 
      aaaatccat gacaggggg cttaattaa atcggcacg 
      tttaatgcg taagaagcg tgatgatgt tatgctctg 
      aaaaaaatc aactccagg tttattaag cgatccatt 
      atccaccct ccactcacg attttactt ttatttaca 
      cacagaatg gacagagtg gataacaat aattacaaa 
      aaatatcgt gtaagtaca cacattcgg tcaaaatac 
      gtgcataaa aatagaaag gaactgcag ggtgcagac 
      ggcgttttt taaatttta gaaatacga cgaaaatcg 
      tatagtaat cgtgttaaa atataaata gtacaagaa 
      a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY