| General information | |||
| TC003342 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 1503064..1643110 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC003342 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_00557 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)Days post injection
Analysis of larval stage 1 cuticle
number of eggs/embryos/larvae on slide: < 50
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
Days post injection
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 4 (16 dpi)
Days post injection
eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 3 (16 dpi)
Days post injection
adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
Days post injection
Pupal morphology
- >TC003342 RA
MMTFPGILS GASVLAIII LATTGQVLS NDPPEITTK PRNLQVKAG GIAAFYCAA RGDPRPVIQ WKKNGKKVS GSQTRYQVK EFPDGGSIL RIEPVKAGR DDANYECVA ENGVGDAVN ADATLVVFE TDKLPPGFP QITQSPTTN KVVEIGHKA VLSCAATGN PPPKITWLK NMLPINSST SPRYTIRDE MPGTLQIRD SEEKDQGKY ECMAENAIG TDYSKSAQL YVKVRRVSP QFSIPPAPL SEVMLGANL NLTCVAVGS PMPFVKWRK GLTQDLTPE DKLPIGKNT LQLTNIQES ANYTCIAAS VLGVIDAVA QVKVQSLPG PPINVRASE ITATSVRLT WSYNGPEDL QYYVIQFKP KYANQAFSE ISGIITMYY TIRSLSPYT EYEMYVIAV NNIGRGPPS APEFVTTGE TAETSFGGA KPGSSPRNV QVRPLSSST MVIQWDEPE TANGQVTGY KVYYTTNSQ LPMAQWESQ VVDNNQLTT ISELTPHTI YTIRVQAFT SVGPGPLSA PVHVKTQQG VPSQPSNLM ASDIGETSV TLQWSKPTH SGENIVNYE LYWNDTYAK EKHHRRIPI TESYTLTGL YPNTLYYVW LAARSQRGE GATTPPIPV RTKQYVPGA PPSNVTGEA VSPTAIRVT WEPPLANRS HGNIIYYKL QYVEADRSD SEAIEVKMN ATSFVLDEL KRWTTYRIW VLAGTSVGD GPSSFPITV RTHEDVPGN PEDVKVTPI NSTTIKVEW KPPHPKERN GIILGYHVH VQETKEEGK NFLNDPMKF DVFGDAVLD LNVSGLQPD TTYAVQVAA LTRKGDGDR SPPVNVRTP GGVPNRPAL TIKIIEREP TVTIELEWT RPSQTYGEL KGYRLRFGV KDQKLKDVN LKGTHTNSY RISNLERGV EYEFRVAGQ NHIGIGQEA IKYMHTPEG PPTGPPSNI THRFQTPDV VCVTWDPPA REHRNGQII KYDIQFHKK SDHSNIITR NVSTTKAVF TNLEENTEY VFHVKAYTN QGAGPNSEK FTIQTDRDI GRAPLSVKA VATSDSSVE VWWEPVPSR AKIIGYQIF YTMTAVEDL DEWQQKSVD VTESADLVN LEKYAQYAI AVAARFKNG LGRLSEKVT VKVKPEDVP LNLRAHEVS THSMTLTWS PPIRLNPIK YKISFDAIK EFVDSQGIT QTQKIERNE ILLADDART YTINNLSPF TTYNVNVSA IPTDHSYRP PTKITVTTQ MAAPQPMVK PDFYGVVNG EEIHVILPQ ASEEYGPIS HYYLVVVPE TKEQANMNP DQYLTNNLI ENKEKAASN HDLPYIAAK FPQRNIPYT FHLGTGEDN DEFTNYKLQ RGKRYRIFV RAVVDTPQK HLYTSSPFS EYLSLDMRE VPPGDPPLR PNPNVPTDN DVKVSSQRK EAGLFWIIV PVIAALLLS LIFVLVFIY RKRRQPCKT PDQAAVTRP LIPADLNNS IAPSDPVAM RRLNFQTPA MISHPPIPI SELANHIER LKANDNLKF SQEYESIEP GQQFTWDHS NMDVNKPKN RYANVIAYD HSRVILTPE EGVLGSDYI NANYCDGYR KHNAYVATQ GPLQETFGD FWRMCWELK TATIVMMTK LEERTRIKC DQYWPTRGS ETYGNMNIT ITDVQELAT YCIRTFQIT KQGGLNERR EVKQLQFTA WPDHGVPDH PAPFLQFLR RVSSLNPLD AGPVVVHCS AGVGRTGCF IVIDSMLER MRHEKTVDI YGHVTCLRA QRNYMVQTE DQYIFIHDA LLEAVICGQ TEVPARNLQ SHIQKLMQL EPGENVTGM EHEFKKLGN IKTDSSRFV TANLPCNKH KNRLVHILP YESTRVCLA PIRGIEGSD YINASFIDG YRYRKAYIA TQGPLPDTT DDLWRMLWE HNSTIIVML TKLKEMGRE KCHQYWPND RSVRYQCFV VDPIAEYNM PQYILREFK VTDAREGSS RTVRQFQFT DWPEQGVPK YGDGFIDFI GQVHKTKEQ FGQDGPITV HCSAGVGRT GVFITLSIA LERMQYEGV VDIFQTARI LRTQRPAMV QTEDQYQFC YRAALEYLG SFDHYTN
- >TC003342 RB
MFCWLCVAE ACSNAASAT TAAVPTISS TYWLSESTS QPVDDDVPR NFIGGFSLG HHHPRNYRP SPLQRASRW ICCLNVLKR FMKRRDYPQ VCELLSESV LIPVTVYVP DPPEITTKP RNLQVKAGG IAAFYCAAR GDPRPVIQW KKNGKKVSG SQTRYQVKE FPDGGSILR IEPVKAGRD DANYECVAE NGVGDAVNA DATLVVFET DKLPPGFPQ ITQSPTTNK VVEIGHKAV LSCAATGNP PPKITWLKN MLPINSSTS PRYTIRDEM PGTLQIRDS EEKDQGKYE CMAENAIGT DYSKSAQLY VKVRRVSPQ FSIPPAPLS EVMLGANLN LTCVAVGSP MPFVKWRKG LTQDLTPED KLPIGKNTL QLTNIQESA NYTCIAASV LGVIDAVAQ VKVQSLPGP PINVRASEI TATSVRLTW SYNGPEDLQ YYVIQFKPK YANQAFSEI SGIITMYYT IRSLSPYTE YEMYVIAVN NIGRGPPSA PEFVTTGET AETSFGGAK PGSSPRNVQ VRPLSSSTM VIQWDEPET ANGQVTGYK VYYTTNSQL PMAQWESQV VDNNQLTTI SELTPHTIY TIRVQAFTS VGPGPLSAP VHVKTQQGV PSQPSNLMA SDIGETSVT LQWSKPTHS GENIVNYEL YWNDTYAKE KHHRRIPIT ESYTLTGLY PNTLYYVWL AARSQRGEG ATTPPIPVR TKQYVPGAP PSNVTGEAV SPTAIRVTW EPPLANRSH GNIIYYKLQ YVEADRSDS EAIEVKMNA TSFVLDELK RWTTYRIWV LAGTSVGDG PSSFPITVR THEDVPGNP EDVKVTPIN STTIKVEWK PPHPKERNG IILGYHVHV QETKEEGKN FLNDPMKFD VFGDAVLDL NVSGLQPDT TYAVQVAAL TRKGDGDRS PPVNVRTPG GVPNRPALT IKIIEREPT VTIELEWTR PSQTYGELK GYRLRFGVK DQKLKDVNL KGTHTNSYR ISNLERGVE YEFRVAGQN HIGIGQEAI KYMHTPEGP PTGPPSNIT HRFQTPDVV CVTWDPPAR EHRNGQIIK YDIQFHKKS DHSNIITRN VSTTKAVFT NLEENTEYV FHVKAYTNQ GAGPNSEKF TIQTDRDIG RAPLSVKAV ATSDSSVEV WWEPVPSRA KIIGYQIFY TMTAVEDLD EWQQKSVDV TESADLVNL EKYAQYAIA VAARFKNGL GRLSEKVTV KVKPEDVPL NLRAHEVST HSMTLTWSP PIRLNPIKY KISFDAIKE FVDSQGITQ TQKIERNEI LLADDARTY TINNLSPFT TYNVNVSAI PTDHSYRPP TKITVTTQM AAPQPMVKP DFYGVVNGE EIHVILPQA SEEYGPISH YYLVVVPET KEQANMNPD QYLTNNLIE NKEKAASNH DLPYIAAKF PQRNIPYTF HLGTGEDND EFTNYKLQR GKRYRIFVR AVVDTPQKH LYTSSPFSE YLSLDMREV PPGDPPLRP NPNVPTDND VKVSSQRKE AGLFWIIVP VIAALLLSL IFVLVFIYR KRRQPCKTP DQAAVTRPL IPADLNNSI APSDPVAMR RLNFQTPAM ISHPPIPIS ELANHIERL KANDNLKFS QEYESIEPG QQFTWDHSN MDVNKPKNR YANVIAYDH SRVILTPEE GVLGSDYIN ANYCDGYRK HNAYVATQG PLQETFGDF WRMCWELKT ATIVMMTKL EERTRIKCD QYWPTRGSE TYGNMNITI TDVQELATY CIRTFQITK QGGLNERRE VKQLQFTAW PDHGVPDHP APFLQFLRR VSSLNPLDA GPVVVHCSA GVGRTGCFI VIDSMLERM RHEKTVDIY GHVTCLRAQ RNYMVQTED QYIFIHDAL LEAVICGQT EVPARNLQS HIQKLMQLE PGENVTGME HEFKKLGNI KTDSSRFVT ANLPCNKHK NRLVHILPY ESTRVCLAP IRGIEGSDY INASFIDGY RYRKAYIAT QGPLPDTTD DLWRMLWEH NSTIIVMLT KLKEMGREK CHQYWPNDR SVRYQCFVV DPIAEYNMP QYILREFKV TDAREGSSR TVRQFQFTD WPEQGVPKY GDGFIDFIG QVHKTKEQF GQDGPITVH CSAGVGRTG VFITLSIAL ERMQYEGVV DIFQTARIL RTQRPAMVQ TEDQYQFCY RAALEYLGS FDHYTN
- >TC003342 RA
atgatgacg ttccccgga attttatcg ggggcttca gtcttggcc atcatcatc ctcgcaact acaggccaa gtcctctcc aacgatcct cccgaaatc accacgaag cctcgcaac ctccaagtc aaggccggt ggcatagcg gctttttac tgtgcagct agaggtgat ccacggcct gtcatccaa tggaaaaag aacgggaaa aaggtgtca ggttcccag acgcgatat caagtgaaa gaatttcca gacggtgga tctatactg cgaatcgaa cctgttaaa gccggaaga gacgatgcc aactacgaa tgcgttgcc gaaaacggc gtcggagac gccgtcaac gccgacgcc acgttggtc gtcttcgaa accgataaa ttgccgccg ggcttcccc caaatcacc cagtcgccc accaccaac aaggtcgtc gaaattggg cacaaagct gtgctttcg tgcgccgcc actggtaat ccgccgccc aagatcacc tggctgaag aacatgctg cccatcaac agctccacc agcccacgg tacaccatc agggacgaa atgccaggc accctccaa attagagat agcgaagaa aaagaccaa ggcaagtac gagtgcatg gcggaaaac gcgatcggt accgactac tccaagtca gctcaactc tacgtcaaa gtgcgtcgt gtttctccc caattttcg atacctcca gcgccgctg agtgaagtg atgttgggt gctaatctg aacttgact tgtgtggca gtgggttcg ccgatgcct ttcgtcaag tggcgtaaa ggactcact caggaccta actcctgaa gacaagtta cccattggg aagaacact ttgcaacta actaatatt caagagtct gctaattat acttgtata gccgctagc gtgttgggc gttatcgat gctgttgct caggtcaaa gtacaatca ttaccggga ccacctatc aatgttcga gcgtcagaa atcacagcg acttccgtt cggctgact tggtcctac aacggaccg gaggatctt cagtattac gtcattcaa tttaagcca aaatatgcc aatcaagcc ttcagcgaa atatcagga atcattacc atgtattat acgattaga agccttagt ccctacacc gaatacgaa atgtacgtc attgccgtc aacaatatt gggagaggg cctccgagc gcaccggag tttgttacc acaggagaa acagctgaa acgtctttt ggaggtgcc aaacccgga tcgtcacca cgaaacgtg caagtccga ccactgagt tccagtacc atggtcatt caatgggac gaacccgaa acggcaaac ggacaagtt accggatac aaagtatac tacacaacc aactcccaa ctcccgatg gcccaatgg gaatcccaa gtcgtcgac aacaatcaa ctaacaaca atcagcgaa ctaactcct cacactatc tacaccatc cgtgtccag gccttcact tcagtaggt cctggcccg ctcagtgcc cccgtccac gtaaaaacg caacaaggc gtcccaagc caaccgagc aacctgatg gcgtcggat atcggcgag acctccgtc accctacag tggagcaaa ccgacgcat tccggggaa aacatcgtc aattacgag ctctactgg aatgacacg tacgccaaa gagaaacac caccgtcgc atacctatc accgaatcc tacacattg acgggactt tatcccaac actctgtat tacgtgtgg ttggcggcg agatcgcaa cgtggggaa ggcgccacc acacccccg ataccagtc agaacgaaa cagtacgta ccgggcgct ccgcccagc aacgttacc ggtgaggcg gtaagtccg actgccatc cgagtcacc tgggagccg ccgcttgct aaccgcagt catggaaac attatatac tacaagctg cagtatgtc gaagcagat agatctgat agcgaagct atagaagtt aaaatgaac gctactagt tttgtgctt gacgaactt aaaagatgg accacttac cgaatctgg gtcctggct ggcacctca gtcggggac ggacccagc tcattcccc atcaccgtg cgaacccat gaagatgtt cccggcaac cccgaagac gtaaaagtc accccaatc aattcaacc acgatcaaa gtcgaatgg aaaccgccc catccgaaa gaacgcaac ggcattatc ctgggttac cacgtccac gtccaggaa accaaagaa gaaggcaaa aactttctc aacgatccg atgaagttc gacgttttt ggagacgct gttttggat cttaacgta tcgggattg cagcctgat accacctac gccgtacaa gtggcagct ctgacgagg aaaggggac ggcgacagg agtcctccg gtcaacgtg cgaactccc ggaggagtg cccaataga cccgccttg accataaag atcattgaa cgggaacca acggttacg atagagctg gagtggacc agaccttcg caaacttac ggcgagttg aaggggtac agactgagg ttcggggtg aaggaccaa aagttgaaa gatgtcaat cttaaaggc acccacacc aactcgtac cgcatcagc aacctggaa cgcggcgtc gaatacgaa ttccgtgta gccggccag aaccacatc ggcataggc caggaggcc ataaagtac atgcacaca cctgaaggc cccccaacc ggccctccc agcaacatc acccaccgc ttccaaacc cctgacgtg gtctgcgtt acatgggat cccccagct cgggaacac cgcaacggc caaatcatc aaatacgac atacaattc cacaaaaaa tccgaccat agcaatatc atcactcgc aacgtttcg accaccaag gcagttttc accaacttg gaggagaac accgagtat gtgttccac gtgaaggct tacacgaac caaggcgcc ggccctaat agtgaaaaa tttacaatt caaacggac cgtgatatt ggcagagcg ccactgtcg gtcaaagcg gtggcaacg tccgattcg agcgtagaa gtgtggtgg gagccggtt ccctcacgg gcgaagata atcggttac cagattttc tacaccatg actgcggtc gaagacttg gacgagtgg cagcagaaa tcggtcgat gtgaccgaa tctgccgat ttggtcaat ttggagaaa tatgcacag tatgccatt gcagtcgct gcgaggttt aagaacgga ctcggacgc ctgtctgag aaagtcacc gttaaggtg aaaccggag gacgtgccg ttgaattta cgggcacac gaagtgtcc acgcattcg atgaccttg acttggtcg cccccaatt cgtctgaac ccgatcaag tacaagata tcgttcgat gccattaag gagttcgtc gattcgcaa ggaatcacc caaacgcag aaaatcgaa cgtaacgaa atattgcta gccgatgac gctcggacc tacacaatt aataatctc tcgcccttt accacgtac aacgtcaat gttagtgcc attccaacg gatcattcg taccgacct cccactaaa atcacagtc acgactcaa atggcagcc ccacagcca atggtcaag cccgacttt tacggagtc gtgaacggg gaggaaatc cacgttatt ttaccccaa gcctccgaa gaatacggc cccataagt cactactat ctagtcgtg gtgcccgaa acaaaggaa caagccaac atgaaccct gaccaatac ttgacgaat aatttgatt gagaataag gagaaggcg gctagtaac cacgacctg ccttatatc gctgccaaa ttcccgcag agaaacatc ccgtacacc ttccatctc gggactggc gaagacaat gacgagttt acaaattac aagttacag agggggaaa cgctacagg attttcgtg cgtgctgtg gtggacacg ccccagaag cacctttac accagcagc cccttttcc gagtaccta tcgctcgac atgcgcgag gtgcccccc ggcgacccg ccactgcgc cccaacccc aacgtcccg accgacaac gacgtgaaa gtgagctcg caacgcaag gaagctgga cttttctgg atcatagtt cccgtaatc gccgccttg ttgctatcc ctcattttc gtcctcgtg ttcatatac cgcaagcgg cgccagccg tgcaaaacc cccgatcag gccgccgtg acacgtcct ttaattccg gccgattta aataatagt atagcgccc agcgatcct gtggcaatg cgacgcctc aacttccaa acacctgcc atgatttcg catccgccg atccccatc tcggagcta gcgaatcat atcgaacgc cttaaagcc aacgacaat ctcaagttt tcgcaagag tacgagagc atagagccg ggacagcag ttcacgtgg gaccactcc aatatggat gtgaataag ccgaagaat cggtatgct aatgtgatc gcgtacgat cacagtcgc gtgattttg acgcccgag gagggtgta ttagggagt gattatatc aatgcgaat tattgtgac gggtatcgg aagcataat gcgtatgtg gcgacgcaa gggccgcta caggagacc tttggtgat ttttggcgg atgtgttgg gagttgaaa acagcgacg attgttatg atgaccaag ttggaggaa cggacgagg attaagtgt gaccagtat tggccaacg agagggtcg gagacgtac ggcaatatg aatattacg ataactgac gttcaggag ttggcaacg tactgtatt cgcacgttc cagattacg aaacaggga ggactaaac gagcgtcgt gaggtgaag caactgcag ttcaccgca tggcctgac cacggcgtg cccgaccac ccagccccc ttcctccag ttcctccgg agggtgtcc agcctcaac ccgctggac gccggccct gtggtggtg cactgttca gctggcgta ggtcgcacc ggttgtttc atcgtcatt gactcaatg ctggagcga atgcggcac gaaaaaacc gtcgatatt tacggacac gtgacgtgt ttgcgcgcc cagaggaac tacatggtg cagaccgaa gaccagtac attttcatc cacgatgcg ttgctcgaa gctgtgatt tgcggacag acggaggtg ccagctcgc aatttgcag tcgcatatc cagaagctg atgcaactg gagccgggc gagaacgtg accgggatg gagcacgag ttcaagaag ttgggcaat atcaagact gattcgtca cggttcgtt acggcgaat ttgccgtgc aataaacat aagaataga ttggtacat atcttgccg tatgagagt acgagagtg tgtttggcg ccgataaga gggatcgaa ggctcggat tatatcaat gcgagtttt atcgatggg tataggtat aggaaagcg tatattgcg acgcagggg ccgctgccg gacaccacg gatgatctg tggaggatg ctgtgggag cacaactcg acgattatt gtcatgctg acgaaactc aaggaaatg ggacgggag aaatgccat caatactgg cccaacgac cgatccgtg cgctaccag tgtttcgtc gttgacccg atcgccgag tacaacatg ccccagtac attcttcgg gaattcaaa gtaaccgat gctcgtgaa ggctcgtca cgaaccgtt aggcaattc cagttcact gattggcca gaacagggg gttcccaaa tacggagat ggtttcatc gacttcatt gggcaagtg cacaagact aaagaacaa ttcggccaa gatggacca attactgtc cactgcagc gctggtgtt ggccgaact ggcgttttc atcacgttg agtatagcg ttagaacgg atgcaatac gaaggagtc gttgatatt ttccaaacg gcgagaatt ttacgcacg caaaggccg gccatggtc caaaccgag gaccagtac cagttttgt tatcgggca gctttggaa tacttgggc tcttttgat cattacacc aactga
- >TC003342 RB
atgttttgt tggttatgt gtcgcggag gcgtgttcg aacgcggcg tccgccacc actgctgct gtcccaacg atcagcagc acgtattgg ttgtcggaa tcgacgtca cagccggta gatgatgac gttccccgg aattttatc gggggcttc agtcttggc catcatcat cctcgcaac tacaggcca agtcctctc caacgtgcg tcgcggtgg atctgctgt ttgaacgtc ttaaagcgc tttatgaaa cggcgagat tacccccag gtgtgcgag ctgttgtcg gagtctgtc ttaatcccg gttactgtt tatgttcca gatcctccc gaaatcacc acgaagcct cgcaacctc caagtcaag gccggtggc atagcggct ttttactgt gcagctaga ggtgatcca cggcctgtc atccaatgg aaaaagaac gggaaaaag gtgtcaggt tcccagacg cgatatcaa gtgaaagaa tttccagac ggtggatct atactgcga atcgaacct gttaaagcc ggaagagac gatgccaac tacgaatgc gttgccgaa aacggcgtc ggagacgcc gtcaacgcc gacgccacg ttggtcgtc ttcgaaacc gataaattg ccgccgggc ttcccccaa atcacccag tcgcccacc accaacaag gtcgtcgaa attgggcac aaagctgtg ctttcgtgc gccgccact ggtaatccg ccgcccaag atcacctgg ctgaagaac atgctgccc atcaacagc tccaccagc ccacggtac accatcagg gacgaaatg ccaggcacc ctccaaatt agagatagc gaagaaaaa gaccaaggc aagtacgag tgcatggcg gaaaacgcg atcggtacc gactactcc aagtcagct caactctac gtcaaagtg cgtcgtgtt tctccccaa ttttcgata cctccagcg ccgctgagt gaagtgatg ttgggtgct aatctgaac ttgacttgt gtggcagtg ggttcgccg atgcctttc gtcaagtgg cgtaaagga ctcactcag gacctaact cctgaagac aagttaccc attgggaag aacactttg caactaact aatattcaa gagtctgct aattatact tgtatagcc gctagcgtg ttgggcgtt atcgatgct gttgctcag gtcaaagta caatcatta ccgggacca cctatcaat gttcgagcg tcagaaatc acagcgact tccgttcgg ctgacttgg tcctacaac ggaccggag gatcttcag tattacgtc attcaattt aagccaaaa tatgccaat caagccttc agcgaaata tcaggaatc attaccatg tattatacg attagaagc cttagtccc tacaccgaa tacgaaatg tacgtcatt gccgtcaac aatattggg agagggcct ccgagcgca ccggagttt gttaccaca ggagaaaca gctgaaacg tcttttgga ggtgccaaa cccggatcg tcaccacga aacgtgcaa gtccgacca ctgagttcc agtaccatg gtcattcaa tgggacgaa cccgaaacg gcaaacgga caagttacc ggatacaaa gtatactac acaaccaac tcccaactc ccgatggcc caatgggaa tcccaagtc gtcgacaac aatcaacta acaacaatc agcgaacta actcctcac actatctac accatccgt gtccaggcc ttcacttca gtaggtcct ggcccgctc agtgccccc gtccacgta aaaacgcaa caaggcgtc ccaagccaa ccgagcaac ctgatggcg tcggatatc ggcgagacc tccgtcacc ctacagtgg agcaaaccg acgcattcc ggggaaaac atcgtcaat tacgagctc tactggaat gacacgtac gccaaagag aaacaccac cgtcgcata cctatcacc gaatcctac acattgacg ggactttat cccaacact ctgtattac gtgtggttg gcggcgaga tcgcaacgt ggggaaggc gccaccaca cccccgata ccagtcaga acgaaacag tacgtaccg ggcgctccg cccagcaac gttaccggt gaggcggta agtccgact gccatccga gtcacctgg gagccgccg cttgctaac cgcagtcat ggaaacatt atatactac aagctgcag tatgtcgaa gcagataga tctgatagc gaagctata gaagttaaa atgaacgct actagtttt gtgcttgac gaacttaaa agatggacc acttaccga atctgggtc ctggctggc acctcagtc ggggacgga cccagctca ttccccatc accgtgcga acccatgaa gatgttccc ggcaacccc gaagacgta aaagtcacc ccaatcaat tcaaccacg atcaaagtc gaatggaaa ccgccccat ccgaaagaa cgcaacggc attatcctg ggttaccac gtccacgtc caggaaacc aaagaagaa ggcaaaaac tttctcaac gatccgatg aagttcgac gtttttgga gacgctgtt ttggatctt aacgtatcg ggattgcag cctgatacc acctacgcc gtacaagtg gcagctctg acgaggaaa ggggacggc gacaggagt cctccggtc aacgtgcga actcccgga ggagtgccc aatagaccc gccttgacc ataaagatc attgaacgg gaaccaacg gttacgata gagctggag tggaccaga ccttcgcaa acttacggc gagttgaag gggtacaga ctgaggttc ggggtgaag gaccaaaag ttgaaagat gtcaatctt aaaggcacc cacaccaac tcgtaccgc atcagcaac ctggaacgc ggcgtcgaa tacgaattc cgtgtagcc ggccagaac cacatcggc ataggccag gaggccata aagtacatg cacacacct gaaggcccc ccaaccggc cctcccagc aacatcacc caccgcttc caaacccct gacgtggtc tgcgttaca tgggatccc ccagctcgg gaacaccgc aacggccaa atcatcaaa tacgacata caattccac aaaaaatcc gaccatagc aatatcatc actcgcaac gtttcgacc accaaggca gttttcacc aacttggag gagaacacc gagtatgtg ttccacgtg aaggcttac acgaaccaa ggcgccggc cctaatagt gaaaaattt acaattcaa acggaccgt gatattggc agagcgcca ctgtcggtc aaagcggtg gcaacgtcc gattcgagc gtagaagtg tggtgggag ccggttccc tcacgggcg aagataatc ggttaccag attttctac accatgact gcggtcgaa gacttggac gagtggcag cagaaatcg gtcgatgtg accgaatct gccgatttg gtcaatttg gagaaatat gcacagtat gccattgca gtcgctgcg aggtttaag aacggactc ggacgcctg tctgagaaa gtcaccgtt aaggtgaaa ccggaggac gtgccgttg aatttacgg gcacacgaa gtgtccacg cattcgatg accttgact tggtcgccc ccaattcgt ctgaacccg atcaagtac aagatatcg ttcgatgcc attaaggag ttcgtcgat tcgcaagga atcacccaa acgcagaaa atcgaacgt aacgaaata ttgctagcc gatgacgct cggacctac acaattaat aatctctcg ccctttacc acgtacaac gtcaatgtt agtgccatt ccaacggat cattcgtac cgacctccc actaaaatc acagtcacg actcaaatg gcagcccca cagccaatg gtcaagccc gacttttac ggagtcgtg aacggggag gaaatccac gttatttta ccccaagcc tccgaagaa tacggcccc ataagtcac tactatcta gtcgtggtg cccgaaaca aaggaacaa gccaacatg aaccctgac caatacttg acgaataat ttgattgag aataaggag aaggcggct agtaaccac gacctgcct tatatcgct gccaaattc ccgcagaga aacatcccg tacaccttc catctcggg actggcgaa gacaatgac gagtttaca aattacaag ttacagagg gggaaacgc tacaggatt ttcgtgcgt gctgtggtg gacacgccc cagaagcac ctttacacc agcagcccc ttttccgag tacctatcg ctcgacatg cgcgaggtg ccccccggc gacccgcca ctgcgcccc aaccccaac gtcccgacc gacaacgac gtgaaagtg agctcgcaa cgcaaggaa gctggactt ttctggatc atagttccc gtaatcgcc gccttgttg ctatccctc attttcgtc ctcgtgttc atataccgc aagcggcgc cagccgtgc aaaaccccc gatcaggcc gccgtgaca cgtccttta attccggcc gatttaaat aatagtata gcgcccagc gatcctgtg gcaatgcga cgcctcaac ttccaaaca cctgccatg atttcgcat ccgccgatc cccatctcg gagctagcg aatcatatc gaacgcctt aaagccaac gacaatctc aagttttcg caagagtac gagagcata gagccggga cagcagttc acgtgggac cactccaat atggatgtg aataagccg aagaatcgg tatgctaat gtgatcgcg tacgatcac agtcgcgtg attttgacg cccgaggag ggtgtatta gggagtgat tatatcaat gcgaattat tgtgacggg tatcggaag cataatgcg tatgtggcg acgcaaggg ccgctacag gagaccttt ggtgatttt tggcggatg tgttgggag ttgaaaaca gcgacgatt gttatgatg accaagttg gaggaacgg acgaggatt aagtgtgac cagtattgg ccaacgaga gggtcggag acgtacggc aatatgaat attacgata actgacgtt caggagttg gcaacgtac tgtattcgc acgttccag attacgaaa cagggagga ctaaacgag cgtcgtgag gtgaagcaa ctgcagttc accgcatgg cctgaccac ggcgtgccc gaccaccca gcccccttc ctccagttc ctccggagg gtgtccagc ctcaacccg ctggacgcc ggccctgtg gtggtgcac tgttcagct ggcgtaggt cgcaccggt tgtttcatc gtcattgac tcaatgctg gagcgaatg cggcacgaa aaaaccgtc gatatttac ggacacgtg acgtgtttg cgcgcccag aggaactac atggtgcag accgaagac cagtacatt ttcatccac gatgcgttg ctcgaagct gtgatttgc ggacagacg gaggtgcca gctcgcaat ttgcagtcg catatccag aagctgatg caactggag ccgggcgag aacgtgacc gggatggag cacgagttc aagaagttg ggcaatatc aagactgat tcgtcacgg ttcgttacg gcgaatttg ccgtgcaat aaacataag aatagattg gtacatatc ttgccgtat gagagtacg agagtgtgt ttggcgccg ataagaggg atcgaaggc tcggattat atcaatgcg agttttatc gatgggtat aggtatagg aaagcgtat attgcgacg caggggccg ctgccggac accacggat gatctgtgg aggatgctg tgggagcac aactcgacg attattgtc atgctgacg aaactcaag gaaatggga cgggagaaa tgccatcaa tactggccc aacgaccga tccgtgcgc taccagtgt ttcgtcgtt gacccgatc gccgagtac aacatgccc cagtacatt cttcgggaa ttcaaagta accgatgct cgtgaaggc tcgtcacga accgttagg caattccag ttcactgat tggccagaa cagggggtt cccaaatac ggagatggt ttcatcgac ttcattggg caagtgcac aagactaaa gaacaattc ggccaagat ggaccaatt actgtccac tgcagcgct ggtgttggc cgaactggc gttttcatc acgttgagt atagcgtta gaacggatg caatacgaa ggagtcgtt gatattttc caaacggcg agaatttta cgcacgcaa aggccggcc atggtccaa accgaggac cagtaccag ttttgttat cgggcagct ttggaatac ttgggctct tttgatcat tacaccaac tga
- >TC003342 RA
ttcgatttt tttttcaga tcagcagca cgtattggt tgtcggaat cgacgtcac agccggtag atgatgacg ttccccgga attttatcg ggggcttca gtcttggcc atcatcatc ctcgcaact acaggccaa gtcctctcc aacgatcct cccgaaatc accacgaag cctcgcaac ctccaagtc aaggccggt ggcatagcg gctttttac tgtgcagct agaggtgat ccacggcct gtcatccaa tggaaaaag aacgggaaa aaggtgtca ggttcccag acgcgatat caagtgaaa gaatttcca gacggtgga tctatactg cgaatcgaa cctgttaaa gccggaaga gacgatgcc aactacgaa tgcgttgcc gaaaacggc gtcggagac gccgtcaac gccgacgcc acgttggtc gtcttcgaa accgataaa ttgccgccg ggcttcccc caaatcacc cagtcgccc accaccaac aaggtcgtc gaaattggg cacaaagct gtgctttcg tgcgccgcc actggtaat ccgccgccc aagatcacc tggctgaag aacatgctg cccatcaac agctccacc agcccacgg tacaccatc agggacgaa atgccaggc accctccaa attagagat agcgaagaa aaagaccaa ggcaagtac gagtgcatg gcggaaaac gcgatcggt accgactac tccaagtca gctcaactc tacgtcaaa gtgcgtcgt gtttctccc caattttcg atacctcca gcgccgctg agtgaagtg atgttgggt gctaatctg aacttgact tgtgtggca gtgggttcg ccgatgcct ttcgtcaag tggcgtaaa ggactcact caggaccta actcctgaa gacaagtta cccattggg aagaacact ttgcaacta actaatatt caagagtct gctaattat acttgtata gccgctagc gtgttgggc gttatcgat gctgttgct caggtcaaa gtacaatca ttaccggga ccacctatc aatgttcga gcgtcagaa atcacagcg acttccgtt cggctgact tggtcctac aacggaccg gaggatctt cagtattac gtcattcaa tttaagcca aaatatgcc aatcaagcc ttcagcgaa atatcagga atcattacc atgtattat acgattaga agccttagt ccctacacc gaatacgaa atgtacgtc attgccgtc aacaatatt gggagaggg cctccgagc gcaccggag tttgttacc acaggagaa acagctgaa acgtctttt ggaggtgcc aaacccgga tcgtcacca cgaaacgtg caagtccga ccactgagt tccagtacc atggtcatt caatgggac gaacccgaa acggcaaac ggacaagtt accggatac aaagtatac tacacaacc aactcccaa ctcccgatg gcccaatgg gaatcccaa gtcgtcgac aacaatcaa ctaacaaca atcagcgaa ctaactcct cacactatc tacaccatc cgtgtccag gccttcact tcagtaggt cctggcccg ctcagtgcc cccgtccac gtaaaaacg caacaaggc gtcccaagc caaccgagc aacctgatg gcgtcggat atcggcgag acctccgtc accctacag tggagcaaa ccgacgcat tccggggaa aacatcgtc aattacgag ctctactgg aatgacacg tacgccaaa gagaaacac caccgtcgc atacctatc accgaatcc tacacattg acgggactt tatcccaac actctgtat tacgtgtgg ttggcggcg agatcgcaa cgtggggaa ggcgccacc acacccccg ataccagtc agaacgaaa cagtacgta ccgggcgct ccgcccagc aacgttacc ggtgaggcg gtaagtccg actgccatc cgagtcacc tgggagccg ccgcttgct aaccgcagt catggaaac attatatac tacaagctg cagtatgtc gaagcagat agatctgat agcgaagct atagaagtt aaaatgaac gctactagt tttgtgctt gacgaactt aaaagatgg accacttac cgaatctgg gtcctggct ggcacctca gtcggggac ggacccagc tcattcccc atcaccgtg cgaacccat gaagatgtt cccggcaac cccgaagac gtaaaagtc accccaatc aattcaacc acgatcaaa gtcgaatgg aaaccgccc catccgaaa gaacgcaac ggcattatc ctgggttac cacgtccac gtccaggaa accaaagaa gaaggcaaa aactttctc aacgatccg atgaagttc gacgttttt ggagacgct gttttggat cttaacgta tcgggattg cagcctgat accacctac gccgtacaa gtggcagct ctgacgagg aaaggggac ggcgacagg agtcctccg gtcaacgtg cgaactccc ggaggagtg cccaataga cccgccttg accataaag atcattgaa cgggaacca acggttacg atagagctg gagtggacc agaccttcg caaacttac ggcgagttg aaggggtac agactgagg ttcggggtg aaggaccaa aagttgaaa gatgtcaat cttaaaggc acccacacc aactcgtac cgcatcagc aacctggaa cgcggcgtc gaatacgaa ttccgtgta gccggccag aaccacatc ggcataggc caggaggcc ataaagtac atgcacaca cctgaaggc cccccaacc ggccctccc agcaacatc acccaccgc ttccaaacc cctgacgtg gtctgcgtt acatgggat cccccagct cgggaacac cgcaacggc caaatcatc aaatacgac atacaattc cacaaaaaa tccgaccat agcaatatc atcactcgc aacgtttcg accaccaag gcagttttc accaacttg gaggagaac accgagtat gtgttccac gtgaaggct tacacgaac caaggcgcc ggccctaat agtgaaaaa tttacaatt caaacggac cgtgatatt ggcagagcg ccactgtcg gtcaaagcg gtggcaacg tccgattcg agcgtagaa gtgtggtgg gagccggtt ccctcacgg gcgaagata atcggttac cagattttc tacaccatg actgcggtc gaagacttg gacgagtgg cagcagaaa tcggtcgat gtgaccgaa tctgccgat ttggtcaat ttggagaaa tatgcacag tatgccatt gcagtcgct gcgaggttt aagaacgga ctcggacgc ctgtctgag aaagtcacc gttaaggtg aaaccggag gacgtgccg ttgaattta cgggcacac gaagtgtcc acgcattcg atgaccttg acttggtcg cccccaatt cgtctgaac ccgatcaag tacaagata tcgttcgat gccattaag gagttcgtc gattcgcaa ggaatcacc caaacgcag aaaatcgaa cgtaacgaa atattgcta gccgatgac gctcggacc tacacaatt aataatctc tcgcccttt accacgtac aacgtcaat gttagtgcc attccaacg gatcattcg taccgacct cccactaaa atcacagtc acgactcaa atggcagcc ccacagcca atggtcaag cccgacttt tacggagtc gtgaacggg gaggaaatc cacgttatt ttaccccaa gcctccgaa gaatacggc cccataagt cactactat ctagtcgtg gtgcccgaa acaaaggaa caagccaac atgaaccct gaccaatac ttgacgaat aatttgatt gagaataag gagaaggcg gctagtaac cacgacctg ccttatatc gctgccaaa ttcccgcag agaaacatc ccgtacacc ttccatctc gggactggc gaagacaat gacgagttt acaaattac aagttacag agggggaaa cgctacagg attttcgtg cgtgctgtg gtggacacg ccccagaag cacctttac accagcagc cccttttcc gagtaccta tcgctcgac atgcgcgag gtgcccccc ggcgacccg ccactgcgc cccaacccc aacgtcccg accgacaac gacgtgaaa gtgagctcg caacgcaag gaagctgga cttttctgg atcatagtt cccgtaatc gccgccttg ttgctatcc ctcattttc gtcctcgtg ttcatatac cgcaagcgg cgccagccg tgcaaaacc cccgatcag gccgccgtg acacgtcct ttaattccg gccgattta aataatagt atagcgccc agcgatcct gtggcaatg cgacgcctc aacttccaa acacctgcc atgatttcg catccgccg atccccatc tcggagcta gcgaatcat atcgaacgc cttaaagcc aacgacaat ctcaagttt tcgcaagag tacgagagc atagagccg ggacagcag ttcacgtgg gaccactcc aatatggat gtgaataag ccgaagaat cggtatgct aatgtgatc gcgtacgat cacagtcgc gtgattttg acgcccgag gagggtgta ttagggagt gattatatc aatgcgaat tattgtgac gggtatcgg aagcataat gcgtatgtg gcgacgcaa gggccgcta caggagacc tttggtgat ttttggcgg atgtgttgg gagttgaaa acagcgacg attgttatg atgaccaag ttggaggaa cggacgagg attaagtgt gaccagtat tggccaacg agagggtcg gagacgtac ggcaatatg aatattacg ataactgac gttcaggag ttggcaacg tactgtatt cgcacgttc cagattacg aaacaggga ggactaaac gagcgtcgt gaggtgaag caactgcag ttcaccgca tggcctgac cacggcgtg cccgaccac ccagccccc ttcctccag ttcctccgg agggtgtcc agcctcaac ccgctggac gccggccct gtggtggtg cactgttca gctggcgta ggtcgcacc ggttgtttc atcgtcatt gactcaatg ctggagcga atgcggcac gaaaaaacc gtcgatatt tacggacac gtgacgtgt ttgcgcgcc cagaggaac tacatggtg cagaccgaa gaccagtac attttcatc cacgatgcg ttgctcgaa gctgtgatt tgcggacag acggaggtg ccagctcgc aatttgcag tcgcatatc cagaagctg atgcaactg gagccgggc gagaacgtg accgggatg gagcacgag ttcaagaag ttgggcaat atcaagact gattcgtca cggttcgtt acggcgaat ttgccgtgc aataaacat aagaataga ttggtacat atcttgccg tatgagagt acgagagtg tgtttggcg ccgataaga gggatcgaa ggctcggat tatatcaat gcgagtttt atcgatggg tataggtat aggaaagcg tatattgcg acgcagggg ccgctgccg gacaccacg gatgatctg tggaggatg ctgtgggag cacaactcg acgattatt gtcatgctg acgaaactc aaggaaatg ggacgggag aaatgccat caatactgg cccaacgac cgatccgtg cgctaccag tgtttcgtc gttgacccg atcgccgag tacaacatg ccccagtac attcttcgg gaattcaaa gtaaccgat gctcgtgaa ggctcgtca cgaaccgtt aggcaattc cagttcact gattggcca gaacagggg gttcccaaa tacggagat ggtttcatc gacttcatt gggcaagtg cacaagact aaagaacaa ttcggccaa gatggacca attactgtc cactgcagc gctggtgtt ggccgaact ggcgttttc atcacgttg agtatagcg ttagaacgg atgcaatac gaaggagtc gttgatatt ttccaaacg gcgagaatt ttacgcacg caaaggccg gccatggtc caaaccgag gaccagtac cagttttgt tatcgggca gctttggaa tacttgggc tcttttgat cattacacc aactgatgg tcccgctaa gtacttgtg ctgttttgt ttctactgc caaattgga cgtttcgag caatgctat gttctaatt taaaccaac tagtgaatt aaattaaat ctaaagaaa aatagtgtg caaatgtgt gtaaactat gtgcctaac ttgtatgtt ggaagatgt aaaaatgaa tctctatag tttgctcta atgtgcaca acatgtgga ctcatgata aaaggacta gtaggaaaa aattttgta tcatataca tatatcgtg caattttca tacctatcg attacgttc cgttgtaca tctttttca gttcgattt ttgatgttg taatcaatc atttttgta cattttagg taatgtaac tcgaataat atatgttat aagactagg acatgttga tttagagaa tttttaata atgttcata tcatttttt ctgtaatcg taactatta caactacac ttaatgaca taccatcct tgaaaatgt taatactta attttctat tgttttcta ttattgtat tgtattgtc tcggcttat taattttta tatctattt acaaacatg ttttatatc taattttta tatattttc acgtgtaat actcaactg tatccacat tagtttgta aacattgtt tatattcaa tgatttgag acgaattgt ttatatttt gtatacatc acgaccgtt gtagcacat gaatgaaat gagactttg atgaaaata aagttctag attttat
- >TC003342 RB
ggccgcccg tgtctttgg ggatgtcag tctgtgtac gtttttttc taattttgt agaaatatc gccggtttt tatttaatt gttagttat gtgtcggtt ttacgtgtg ttcccttta gggactagg gtgcgacca agggtagat atcctgttt ctcacagaa aaaccgtaa gttaaccat gttttgttg gttatgtgt cgcggaggc gtgttcgaa cgcggcgtc cgccaccac tgctgctgt cccaacgat cagcagcac gtattggtt gtcggaatc gacgtcaca gccggtaga tgatgacgt tccccggaa ttttatcgg gggcttcag tcttggcca tcatcatcc tcgcaacta caggccaag tcctctcca acgtgcgtc gcggtggat ctgctgttt gaacgtctt aaagcgctt tatgaaacg gcgagatta cccccaggt gtgcgagct gttgtcgga gtctgtctt aatcccggt tactgttta tgttccaga tcctcccga aatcaccac gaagcctcg caacctcca agtcaaggc cggtggcat agcggcttt ttactgtgc agctagagg tgatccacg gcctgtcat ccaatggaa aaagaacgg gaaaaaggt gtcaggttc ccagacgcg atatcaagt gaaagaatt tccagacgg tggatctat actgcgaat cgaacctgt taaagccgg aagagacga tgccaacta cgaatgcgt tgccgaaaa cggcgtcgg agacgccgt caacgccga cgccacgtt ggtcgtctt cgaaaccga taaattgcc gccgggctt cccccaaat cacccagtc gcccaccac caacaaggt cgtcgaaat tgggcacaa agctgtgct ttcgtgcgc cgccactgg taatccgcc gcccaagat cacctggct gaagaacat gctgcccat caacagctc caccagccc acggtacac catcaggga cgaaatgcc aggcaccct ccaaattag agatagcga agaaaaaga ccaaggcaa gtacgagtg catggcgga aaacgcgat cggtaccga ctactccaa gtcagctca actctacgt caaagtgcg tcgtgtttc tccccaatt ttcgatacc tccagcgcc gctgagtga agtgatgtt gggtgctaa tctgaactt gacttgtgt ggcagtggg ttcgccgat gcctttcgt caagtggcg taaaggact cactcagga cctaactcc tgaagacaa gttacccat tgggaagaa cactttgca actaactaa tattcaaga gtctgctaa ttatacttg tatagccgc tagcgtgtt gggcgttat cgatgctgt tgctcaggt caaagtaca atcattacc gggaccacc tatcaatgt tcgagcgtc agaaatcac agcgacttc cgttcggct gacttggtc ctacaacgg accggagga tcttcagta ttacgtcat tcaatttaa gccaaaata tgccaatca agccttcag cgaaatatc aggaatcat taccatgta ttatacgat tagaagcct tagtcccta caccgaata cgaaatgta cgtcattgc cgtcaacaa tattgggag agggcctcc gagcgcacc ggagtttgt taccacagg agaaacagc tgaaacgtc ttttggagg tgccaaacc cggatcgtc accacgaaa cgtgcaagt ccgaccact gagttccag taccatggt cattcaatg ggacgaacc cgaaacggc aaacggaca agttaccgg atacaaagt atactacac aaccaactc ccaactccc gatggccca atgggaatc ccaagtcgt cgacaacaa tcaactaac aacaatcag cgaactaac tcctcacac tatctacac catccgtgt ccaggcctt cacttcagt aggtcctgg cccgctcag tgcccccgt ccacgtaaa aacgcaaca aggcgtccc aagccaacc gagcaacct gatggcgtc ggatatcgg cgagacctc cgtcaccct acagtggag caaaccgac gcattccgg ggaaaacat cgtcaatta cgagctcta ctggaatga cacgtacgc caaagagaa acaccaccg tcgcatacc tatcaccga atcctacac attgacggg actttatcc caacactct gtattacgt gtggttggc ggcgagatc gcaacgtgg ggaaggcgc caccacacc cccgatacc agtcagaac gaaacagta cgtaccggg cgctccgcc cagcaacgt taccggtga ggcggtaag tccgactgc catccgagt cacctggga gccgccgct tgctaaccg cagtcatgg aaacattat atactacaa gctgcagta tgtcgaagc agatagatc tgatagcga agctataga agttaaaat gaacgctac tagttttgt gcttgacga acttaaaag atggaccac ttaccgaat ctgggtcct ggctggcac ctcagtcgg ggacggacc cagctcatt ccccatcac cgtgcgaac ccatgaaga tgttcccgg caaccccga agacgtaaa agtcacccc aatcaattc aaccacgat caaagtcga atggaaacc gccccatcc gaaagaacg caacggcat tatcctggg ttaccacgt ccacgtcca ggaaaccaa agaagaagg caaaaactt tctcaacga tccgatgaa gttcgacgt ttttggaga cgctgtttt ggatcttaa cgtatcggg attgcagcc tgataccac ctacgccgt acaagtggc agctctgac gaggaaagg ggacggcga caggagtcc tccggtcaa cgtgcgaac tcccggagg agtgcccaa tagacccgc cttgaccat aaagatcat tgaacggga accaacggt tacgataga gctggagtg gaccagacc ttcgcaaac ttacggcga gttgaaggg gtacagact gaggttcgg ggtgaagga ccaaaagtt gaaagatgt caatcttaa aggcaccca caccaactc gtaccgcat cagcaacct ggaacgcgg cgtcgaata cgaattccg tgtagccgg ccagaacca catcggcat aggccagga ggccataaa gtacatgca cacacctga aggcccccc aaccggccc tcccagcaa catcaccca ccgcttcca aacccctga cgtggtctg cgttacatg ggatccccc agctcggga acaccgcaa cggccaaat catcaaata cgacataca attccacaa aaaatccga ccatagcaa tatcatcac tcgcaacgt ttcgaccac caaggcagt tttcaccaa cttggagga gaacaccga gtatgtgtt ccacgtgaa ggcttacac gaaccaagg cgccggccc taatagtga aaaatttac aattcaaac ggaccgtga tattggcag agcgccact gtcggtcaa agcggtggc aacgtccga ttcgagcgt agaagtgtg gtgggagcc ggttccctc acgggcgaa gataatcgg ttaccagat tttctacac catgactgc ggtcgaaga cttggacga gtggcagca gaaatcggt cgatgtgac cgaatctgc cgatttggt caatttgga gaaatatgc acagtatgc cattgcagt cgctgcgag gtttaagaa cggactcgg acgcctgtc tgagaaagt caccgttaa ggtgaaacc ggaggacgt gccgttgaa tttacgggc acacgaagt gtccacgca ttcgatgac cttgacttg gtcgccccc aattcgtct gaacccgat caagtacaa gatatcgtt cgatgccat taaggagtt cgtcgattc gcaaggaat cacccaaac gcagaaaat cgaacgtaa cgaaatatt gctagccga tgacgctcg gacctacac aattaataa tctctcgcc ctttaccac gtacaacgt caatgttag tgccattcc aacggatca ttcgtaccg acctcccac taaaatcac agtcacgac tcaaatggc agccccaca gccaatggt caagcccga cttttacgg agtcgtgaa cggggagga aatccacgt tattttacc ccaagcctc cgaagaata cggccccat aagtcacta ctatctagt cgtggtgcc cgaaacaaa ggaacaagc caacatgaa ccctgacca atacttgac gaataattt gattgagaa taaggagaa ggcggctag taaccacga cctgcctta tatcgctgc caaattccc gcagagaaa catcccgta caccttcca tctcgggac tggcgaaga caatgacga gtttacaaa ttacaagtt acagagggg gaaacgcta caggatttt cgtgcgtgc tgtggtgga cacgcccca gaagcacct ttacaccag cagcccctt ttccgagta cctatcgct cgacatgcg cgaggtgcc ccccggcga cccgccact gcgccccaa ccccaacgt cccgaccga caacgacgt gaaagtgag ctcgcaacg caaggaagc tggactttt ctggatcat agttcccgt aatcgccgc cttgttgct atccctcat tttcgtcct cgtgttcat ataccgcaa gcggcgcca gccgtgcaa aacccccga tcaggccgc cgtgacacg tcctttaat tccggccga tttaaataa tagtatagc gcccagcga tcctgtggc aatgcgacg cctcaactt ccaaacacc tgccatgat ttcgcatcc gccgatccc catctcgga gctagcgaa tcatatcga acgccttaa agccaacga caatctcaa gttttcgca agagtacga gagcataga gccgggaca gcagttcac gtgggacca ctccaatat ggatgtgaa taagccgaa gaatcggta tgctaatgt gatcgcgta cgatcacag tcgcgtgat tttgacgcc cgaggaggg tgtattagg gagtgatta tatcaatgc gaattattg tgacgggta tcggaagca taatgcgta tgtggcgac gcaagggcc gctacagga gacctttgg tgatttttg gcggatgtg ttgggagtt gaaaacagc gacgattgt tatgatgac caagttgga ggaacggac gaggattaa gtgtgacca gtattggcc aacgagagg gtcggagac gtacggcaa tatgaatat tacgataac tgacgttca ggagttggc aacgtactg tattcgcac gttccagat tacgaaaca gggaggact aaacgagcg tcgtgaggt gaagcaact gcagttcac cgcatggcc tgaccacgg cgtgcccga ccacccagc ccccttcct ccagttcct ccggagggt gtccagcct caacccgct ggacgccgg ccctgtggt ggtgcactg ttcagctgg cgtaggtcg caccggttg tttcatcgt cattgactc aatgctgga gcgaatgcg gcacgaaaa aaccgtcga tatttacgg acacgtgac gtgtttgcg cgcccagag gaactacat ggtgcagac cgaagacca gtacatttt catccacga tgcgttgct cgaagctgt gatttgcgg acagacgga ggtgccagc tcgcaattt gcagtcgca tatccagaa gctgatgca actggagcc gggcgagaa cgtgaccgg gatggagca cgagttcaa gaagttggg caatatcaa gactgattc gtcacggtt cgttacggc gaatttgcc gtgcaataa acataagaa tagattggt acatatctt gccgtatga gagtacgag agtgtgttt ggcgccgat aagagggat cgaaggctc ggattatat caatgcgag ttttatcga tgggtatag gtataggaa agcgtatat tgcgacgca ggggccgct gccggacac cacggatga tctgtggag gatgctgtg ggagcacaa ctcgacgat tattgtcat gctgacgaa actcaagga aatgggacg ggagaaatg ccatcaata ctggcccaa cgaccgatc cgtgcgcta ccagtgttt cgtcgttga cccgatcgc cgagtacaa catgcccca gtacattct tcgggaatt caaagtaac cgatgctcg tgaaggctc gtcacgaac cgttaggca attccagtt cactgattg gccagaaca gggggttcc caaatacgg agatggttt catcgactt cattgggca agtgcacaa gactaaaga acaattcgg ccaagatgg accaattac tgtccactg cagcgctgg tgttggccg aactggcgt tttcatcac gttgagtat agcgttaga acggatgca atacgaagg agtcgttga tattttcca aacggcgag aattttacg cacgcaaag gccggccat ggtccaaac cgaggacca gtaccagtt ttgttatcg ggcagcttt ggaatactt gggctcttt tgatcatta caccaactg atggtcccg ctaagtact tgtgctgtt ttgtttcta ctgccaaat tggacgttt cgagcaatg ctatgttct aatttaaac caactagtg aattaaatt aaatctaaa gaaaaatag tgtgcaaat gtgtgtaaa ctatgtgcc taacttgta tgttggaag atgtaaaaa tgaatctct atagtttgc tctaatgtg cacaacatg tggactcat gataaaagg actagtagg aaaaaattt tgtatcata tacatatat cgtgcaatt ttcatacct atcgattac gttccgttg tacatcttt ttcagttcg atttttgat gttgtaatc aatcatttt tgtacattt taggtaatg taactcgaa taatatatg ttataagac taggacatg ttgatttag agaattttt aataatgtt catatcatt ttttctgta atcgtaact attacaact acacttaat gacatacca tccttgaaa atgttaata cttaatttt ctattgttt tctattatt gtattgtat tgtctcggc ttattaatt tttatatct atttacaaa catgtttta tatctaatt tttatatat tttcacgtg taatactca actgtatcc acattagtt tgtaaacat tgtttatat tcaatgatt tgagacgaa ttgtttata ttttgtata catcacgac cgttgtagc acatgaatg aaatgagac tttgatgaa aataaagtt ctagatttt at
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