Status
Please wait ...
Gene information
General information
TC003334 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 1420588..1424652
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003334
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00556

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003334 RA
      MGEPLTNVL TDILKVEGI EEAFSCFLV HRPSAEKEK 
      VAVWQQELN TALRSIPQE QLDTALRQY LNTAVLCSQ 
      SKLQLLMEL LESVVRNGT IGARIVCEV VMNFDKLQH 
      TNARFWIEC FKLVRKIID LVEYKGVRE IMKGCCEKA 
      KSLPWRLNA GLQPQTQAL LSVAERIMD RNACLLPGY 
      LLVNELQKA YPDCPHWRL AALFSDYVG SFRACAQMV 
      AIVGQAEMR PVVEHSGCP EHLVNPWKL DPCTLRFSL 
      KGTLPYAPE LLEKQTGLL RYVLEQPYS RDMVCGMLG 
      LQKQHKRHC AALEEQLVE LVILALERT ENEGDESAT 
      QGLWLHLSS QLIYFVLFQ FASFPNIVI SLHSRLQGR 
      DLRRGRDQL MWVLLQFIS GSIQKNPLS NFLPVLRLY 
      ELLFPEQEP PLPVPDFNQ PQCTRQMAM TCIWIHLTK 
      KAQAEMTNL TWPIPAKLR NHHDFLQQL VPPNNTVLA 
      MGNDYRIAL LCNAYSTNP DYYSKPMAA LVETIQGSP 
      KSTAPPTAP LSMAVLDSL TVHSKMSLI HSIVTHVIK 
      LAQAKLGMP LSPALVETY SRLLVYTEI ESLGIKGFI 
      SQLLPTVYK SHAWGTLYT LLEMFSYRM HHIHPHYRV 
      QLLSHLHSL AAVPQANQT QLHLCVEST ALRLITGLG 
      SGEVQPELS RFLAEPKTL ISAESEELN RALVLTLAR 
      ATHVTGADG SWCHELLAT IAQSTPHAW APHTLECFP 
      RALAEFFTQ HAVPKENKQ QLKKAVEEE YRKWSSMNN 
      ENDMMAHFG VAGAPPLFL CLLWKMLLE TDHITPIAY 
      KILERIGAR ALSAHLRKF CDCLVFEFS NSPGGQHVN 
      KCVDTINDM IWKYNIVTI DRLVLCLAL RTQEGSEAQ 
      VCSFIIQLV LLKATEFRN RVQDFVKDN SPDHWNQTN 
      WHEKHLEFH RKYPEKFAP EEQSSGYHP YFGNVCLRF 
      LPVFDIVVH RFLEIPQVT KSLEILLEH LGCLYKFHD 
      RPVTYLYNT LHYYETRLR DRPPLKRRL VAAVLGTLK 
      DSLSEPYQL YLGRAADEM WTPELDYYI QVVKRVVDI 
      LGGATANSV IDWRFNEFP NAGAHILYT SCVELMALS 
      AGPVAVANG LLDVVAKGY VTIPSSDIH QWVNAVGLI 
      LAALPVSYW SVLHDRLVA TLNELETWN FDCSPFRLF 
      DFKETHAGL LHNNFSYML ALAHSVWHH AGPGQIASV 
      PRWARECLP AVVRSEEQF LYVCHLVGP FLQRFNVAI 
      VELTSALYE LLAQVDHAH SELKYMDPI CDLLYHIKY 
      MFVGDSMKK ELETVVRRL RPPLQMRLR FIAHLAIEE 
      VNAS
    CDS sequence
    • >TC003334 RA
      atgggcgag ccactaaca aacgtccta accgacatt 
      ctcaaagtt gaaggcatc gaagaagcc tttagttgc 
      tttctagtg caccgcccc agtgccgaa aaggaaaag 
      gtggctgta tggcaacaa gagttgaac acagccctg 
      cggagcatc ccccaggag cagctagac accgccctt 
      cgccagtac ctcaacact gctgtacta tgttcccaa 
      agcaagctc cagctcttg atggagctc ctggaaagt 
      gtcgtgcgc aacggtacc attggggcc cgtatcgtg 
      tgtgaggtg gtgatgaat ttcgataaa ctacaacac 
      accaatgcg cggttctgg atcgagtgc tttaaactc 
      gtgcggaaa attatagat ttggttgaa tacaagggc 
      gttcgcgaa attatgaaa ggatgttgc gagaaggcg 
      aaaagtctc ccctggcgc ttgaacgcg ggcttgcag 
      ccccaaacc caggctctt ttaagcgtc gctgaacga 
      atcatggac aggaacgcg tgcctcctg cctggctat 
      ctcctagtg aacgagctc cagaaggcc taccctgac 
      tgcccccac tggcgcctt gctgccctc ttctccgat 
      tacgttggc agttttcgg gcctgtgcc caaatggtg 
      gctatcgta ggccaggcg gaaatgcgg ccagttgtg 
      gagcacagc ggctgtccc gaacacttg gtcaatccc 
      tggaaactt gacccgtgc acgttgcga ttctcgctt 
      aagggcact ttaccgtac gctcccgaa ttactagag 
      aagcaaacc ggtttatta cgatacgtt ctggaacag 
      ccgtacagt cgcgacatg gtgtgtggc atgttaggg 
      ttgcagaaa cagcacaag agacactgt gccgccctt 
      gaggaacaa ctggtcgag ttggtgatt ttggcgctg 
      gaacgcacg gaaaatgag ggcgatgag agcgccact 
      caagggtta tggctgcac ttgtcgtcc cagttaatt 
      tattttgtt cttttccaa tttgcgagt ttcccaaac 
      attgttata tcgttgcat tcgagactg caagggcgc 
      gacttgcgg agagggcgc gaccaactc atgtgggtc 
      ctcttgcag tttatttcc ggttcgatt cagaagaac 
      cccctctcg aatttcctc ccagttttg cggctttac 
      gaactgttg tttccggaa caggagccc cctttgccc 
      gttccggac ttcaaccag ccccagtgt acgagacag 
      atggcaatg acgtgcatt tggatccac ttgactaag 
      aaagcgcaa gccgaaatg accaacttg acttggcca 
      attcccgcc aaactacgc aaccatcat gatttcctc 
      caacagttg gtgccccct aataacacc gtgttagcg 
      atgggaaat gactacaga atcgccttg ttgtgcaac 
      gcctactca acgaacccc gattattac agtaagcca 
      atggcagcg ctagttgag acgatccaa gggagcccc 
      aaatcaaca gccccacct accgcgcct ttaagcatg 
      gccgtcttg gactcatta actgtgcat tcgaaaatg 
      agcttaatc catagtatt gtcacacac gtgattaaa 
      ctagcacag gccaagtta ggaatgcca ctatcacca 
      gctttggtg gaaacgtac agtcgcctg ttggtgtac 
      accgagatc gaatcgttg ggcatcaaa gggttcata 
      agccagttg ttgccaacc gtatacaag tcacacgct 
      tggggcacc ctttacaca cttctggag atgtttagt 
      tatcggatg caccacatc cacccccac taccgtgtc 
      cagttactc agccacttg cattcgttg gcagcggtg 
      ccgcaagcc aaccaaacc caactgcac ttatgtgtc 
      gaatcgacc gctttgcgt ctaatcacg gggctgggc 
      agtggcgaa gttcagccc gaactgagc cgcttttta 
      gccgaaccc aagacgtta atttcggcg gaatcggaa 
      gagttgaat cgggcgctg gttttgacc ctagctagg 
      gccacgcac gtgacgggg gccgacggc tcgtggtgt 
      cacgagttg ctggcgacc attgcacaa agtaccccc 
      catgcgtgg gccccccac accctcgaa tgcttcccc 
      agggctctg gccgagttt ttcacgcaa catgccgtc 
      cctaaagaa aacaaacaa caattgaag aaagcagtg 
      gaggaggag taccggaag tggtcgtcc atgaacaac 
      gaaaatgac atgatggcg cattttggg gttgcggga 
      gcgccccca cttttcctc tgtttgttg tggaaaatg 
      ctcctcgag actgatcac atcacgcca attgcctac 
      aaaatcctg gagaggatt ggggcaagg gcgctttcg 
      gcccacttg aggaagttt tgtgattgt ctcgtgttc 
      gagtttagt aacagtcct ggggggcaa catgttaac 
      aaatgcgtg gatacgatc aatgacatg atctggaag 
      tacaatatt gtgacaatc gatcggttg gtcttgtgt 
      ttggcgctg cggacgcag gaagggagc gaggctcaa 
      gtctgttcg ttcatcatc cagttggtg ttgcttaaa 
      gctacagag tttaggaat cgggtgcag gatttcgtc 
      aaagacaac tcgcccgat cattggaac cagaccaac 
      tggcacgag aaacacttg gagtttcac cggaaatac 
      cccgagaag tttgccccc gaggaacag tcaagtggt 
      taccacccc tattttggc aacgtgtgt ttgcgcttt 
      ttgccggtt tttgatata gtggtgcac cggtttttg 
      gaaataccg caagtcacc aaaagtttg gagattctg 
      ttagaacac ttgggttgt ttgtacaag tttcatgac 
      cgtccggtc acttatttg tacaacact ttgcactat 
      tacgagact aggcttagg gaccgtcca cctttgaaa 
      cgacggttg gtggctgct gtcttaggg acactcaaa 
      gacagtctt tcggagccc taccaactg tacctcgga 
      cgagctgct gacgagatg tggaccccc gaactggac 
      tattacata caagtggtc aagcgagtc gttgatatt 
      ctagggggc gccactgcc aattccgta attgattgg 
      cggttcaat gagttccca aacgccggg gctcatatt 
      ctttacacc agctgtgtg gaactgatg gcgttgtcc 
      gccggtccg gtggccgta gccaacggt ttgctagac 
      gtcgtggcc aaaggctat gtcacaatt ccctcgtca 
      gacatccac cagtgggtc aatgctgtt ggtttaatt 
      ctggcagcg cttccagtt agctactgg agtgttcta 
      cacgatcga cttgtggcc acgttaaac gagctagaa 
      acgtggaat tttgactgt tctcccttc cgtttgttc 
      gatttcaaa gaaacccac gctgggctt ttgcataat 
      aattttagt tatatgcta gctttggct cattcggtt 
      tggcatcac gctggtccg gggcagatc gccagtgtg 
      ccccgatgg gcgcgtgag tgtttaccg gctgtggta 
      cggtccgaa gaacagttt ctctatgtt tgtcatctt 
      gtggggccc tttttgcaa cggtttaac gtggcaata 
      gtggaattg acgagtgct ttgtacgaa ctgttggca 
      caagttgac cacgcgcac agcgagttg aaatatatg 
      gacccgatc tgcgatctc ttgtaccac attaagtac 
      atgtttgtg ggggatagc atgaagaag gagttggag 
      actgtggtg cgccggtta agaccgcct ttgcaaatg 
      aggttgagg tttatcgca catttggcg attgaggaa 
      gttaatgca tcgtaa
    mRNA sequence
    • >TC003334 RA
      agtcggcca aacataacc taccgttat tatcccatt 
      caaataacc acaaaataa tgttttcgc ctgaaaaat 
      aaccgtgga cacgatggg cgagccact aacaaacgt 
      cctaaccga cattctcaa agttgaagg catcgaaga 
      agcctttag ttgctttct agtgcaccg ccccagtgc 
      cgaaaagga aaaggtggc tgtatggca acaagagtt 
      gaacacagc cctgcggag catccccca ggagcagct 
      agacaccgc ccttcgcca gtacctcaa cactgctgt 
      actatgttc ccaaagcaa gctccagct cttgatgga 
      gctcctgga aagtgtcgt gcgcaacgg taccattgg 
      ggcccgtat cgtgtgtga ggtggtgat gaatttcga 
      taaactaca acacaccaa tgcgcggtt ctggatcga 
      gtgctttaa actcgtgcg gaaaattat agatttggt 
      tgaatacaa gggcgttcg cgaaattat gaaaggatg 
      ttgcgagaa ggcgaaaag tctcccctg gcgcttgaa 
      cgcgggctt gcagcccca aacccaggc tcttttaag 
      cgtcgctga acgaatcat ggacaggaa cgcgtgcct 
      cctgcctgg ctatctcct agtgaacga gctccagaa 
      ggcctaccc tgactgccc ccactggcg ccttgctgc 
      cctcttctc cgattacgt tggcagttt tcgggcctg 
      tgcccaaat ggtggctat cgtaggcca ggcggaaat 
      gcggccagt tgtggagca cagcggctg tcccgaaca 
      cttggtcaa tccctggaa acttgaccc gtgcacgtt 
      gcgattctc gcttaaggg cactttacc gtacgctcc 
      cgaattact agagaagca aaccggttt attacgata 
      cgttctgga acagccgta cagtcgcga catggtgtg 
      tggcatgtt agggttgca gaaacagca caagagaca 
      ctgtgccgc ccttgagga acaactggt cgagttggt 
      gattttggc gctggaacg cacggaaaa tgagggcga 
      tgagagcgc cactcaagg gttatggct gcacttgtc 
      gtcccagtt aatttattt tgttctttt ccaatttgc 
      gagtttccc aaacattgt tatatcgtt gcattcgag 
      actgcaagg gcgcgactt gcggagagg gcgcgacca 
      actcatgtg ggtcctctt gcagtttat ttccggttc 
      gattcagaa gaaccccct ctcgaattt cctcccagt 
      tttgcggct ttacgaact gttgtttcc ggaacagga 
      gcccccttt gcccgttcc ggacttcaa ccagcccca 
      gtgtacgag acagatggc aatgacgtg catttggat 
      ccacttgac taagaaagc gcaagccga aatgaccaa 
      cttgacttg gccaattcc cgccaaact acgcaacca 
      tcatgattt cctccaaca gttggtgcc ccctaataa 
      caccgtgtt agcgatggg aaatgacta cagaatcgc 
      cttgttgtg caacgccta ctcaacgaa ccccgatta 
      ttacagtaa gccaatggc agcgctagt tgagacgat 
      ccaagggag ccccaaatc aacagcccc acctaccgc 
      gcctttaag catggccgt cttggactc attaactgt 
      gcattcgaa aatgagctt aatccatag tattgtcac 
      acacgtgat taaactagc acaggccaa gttaggaat 
      gccactatc accagcttt ggtggaaac gtacagtcg 
      cctgttggt gtacaccga gatcgaatc gttgggcat 
      caaagggtt cataagcca gttgttgcc aaccgtata 
      caagtcaca cgcttgggg cacccttta cacacttct 
      ggagatgtt tagttatcg gatgcacca catccaccc 
      ccactaccg tgtccagtt actcagcca cttgcattc 
      gttggcagc ggtgccgca agccaacca aacccaact 
      gcacttatg tgtcgaatc gaccgcttt gcgtctaat 
      cacggggct gggcagtgg cgaagttca gcccgaact 
      gagccgctt tttagccga acccaagac gttaatttc 
      ggcggaatc ggaagagtt gaatcgggc gctggtttt 
      gaccctagc tagggccac gcacgtgac gggggccga 
      cggctcgtg gtgtcacga gttgctggc gaccattgc 
      acaaagtac cccccatgc gtgggcccc ccacaccct 
      cgaatgctt ccccagggc tctggccga gtttttcac 
      gcaacatgc cgtccctaa agaaaacaa acaacaatt 
      gaagaaagc agtggagga ggagtaccg gaagtggtc 
      gtccatgaa caacgaaaa tgacatgat ggcgcattt 
      tggggttgc gggagcgcc cccactttt cctctgttt 
      gttgtggaa aatgctcct cgagactga tcacatcac 
      gccaattgc ctacaaaat cctggagag gattggggc 
      aagggcgct ttcggccca cttgaggaa gttttgtga 
      ttgtctcgt gttcgagtt tagtaacag tcctggggg 
      gcaacatgt taacaaatg cgtggatac gatcaatga 
      catgatctg gaagtacaa tattgtgac aatcgatcg 
      gttggtctt gtgtttggc gctgcggac gcaggaagg 
      gagcgaggc tcaagtctg ttcgttcat catccagtt 
      ggtgttgct taaagctac agagtttag gaatcgggt 
      gcaggattt cgtcaaaga caactcgcc cgatcattg 
      gaaccagac caactggca cgagaaaca cttggagtt 
      tcaccggaa ataccccga gaagtttgc ccccgagga 
      acagtcaag tggttacca cccctattt tggcaacgt 
      gtgtttgcg ctttttgcc ggtttttga tatagtggt 
      gcaccggtt tttggaaat accgcaagt caccaaaag 
      tttggagat tctgttaga acacttggg ttgtttgta 
      caagtttca tgaccgtcc ggtcactta tttgtacaa 
      cactttgca ctattacga gactaggct tagggaccg 
      tccaccttt gaaacgacg gttggtggc tgctgtctt 
      agggacact caaagacag tctttcgga gccctacca 
      actgtacct cggacgagc tgctgacga gatgtggac 
      ccccgaact ggactatta catacaagt ggtcaagcg 
      agtcgttga tattctagg gggcgccac tgccaattc 
      cgtaattga ttggcggtt caatgagtt cccaaacgc 
      cggggctca tattcttta caccagctg tgtggaact 
      gatggcgtt gtccgccgg tccggtggc cgtagccaa 
      cggtttgct agacgtcgt ggccaaagg ctatgtcac 
      aattccctc gtcagacat ccaccagtg ggtcaatgc 
      tgttggttt aattctggc agcgcttcc agttagcta 
      ctggagtgt tctacacga tcgacttgt ggccacgtt 
      aaacgagct agaaacgtg gaattttga ctgttctcc 
      cttccgttt gttcgattt caaagaaac ccacgctgg 
      gcttttgca taataattt tagttatat gctagcttt 
      ggctcattc ggtttggca tcacgctgg tccggggca 
      gatcgccag tgtgccccg atgggcgcg tgagtgttt 
      accggctgt ggtacggtc cgaagaaca gtttctcta 
      tgtttgtca tcttgtggg gcccttttt gcaacggtt 
      taacgtggc aatagtgga attgacgag tgctttgta 
      cgaactgtt ggcacaagt tgaccacgc gcacagcga 
      gttgaaata tatggaccc gatctgcga tctcttgta 
      ccacattaa gtacatgtt tgtggggga tagcatgaa 
      gaaggagtt ggagactgt ggtgcgccg gttaagacc 
      gcctttgca aatgaggtt gaggtttat cgcacattt 
      ggcgattga ggaagttaa tgcatcgta actcacttg 
      tattatgtt tttacattt acgtaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY