| General information | |||
| TC003334 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 1420588..1424652 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC003334 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_00556 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
- >TC003334 RA
MGEPLTNVL TDILKVEGI EEAFSCFLV HRPSAEKEK VAVWQQELN TALRSIPQE QLDTALRQY LNTAVLCSQ SKLQLLMEL LESVVRNGT IGARIVCEV VMNFDKLQH TNARFWIEC FKLVRKIID LVEYKGVRE IMKGCCEKA KSLPWRLNA GLQPQTQAL LSVAERIMD RNACLLPGY LLVNELQKA YPDCPHWRL AALFSDYVG SFRACAQMV AIVGQAEMR PVVEHSGCP EHLVNPWKL DPCTLRFSL KGTLPYAPE LLEKQTGLL RYVLEQPYS RDMVCGMLG LQKQHKRHC AALEEQLVE LVILALERT ENEGDESAT QGLWLHLSS QLIYFVLFQ FASFPNIVI SLHSRLQGR DLRRGRDQL MWVLLQFIS GSIQKNPLS NFLPVLRLY ELLFPEQEP PLPVPDFNQ PQCTRQMAM TCIWIHLTK KAQAEMTNL TWPIPAKLR NHHDFLQQL VPPNNTVLA MGNDYRIAL LCNAYSTNP DYYSKPMAA LVETIQGSP KSTAPPTAP LSMAVLDSL TVHSKMSLI HSIVTHVIK LAQAKLGMP LSPALVETY SRLLVYTEI ESLGIKGFI SQLLPTVYK SHAWGTLYT LLEMFSYRM HHIHPHYRV QLLSHLHSL AAVPQANQT QLHLCVEST ALRLITGLG SGEVQPELS RFLAEPKTL ISAESEELN RALVLTLAR ATHVTGADG SWCHELLAT IAQSTPHAW APHTLECFP RALAEFFTQ HAVPKENKQ QLKKAVEEE YRKWSSMNN ENDMMAHFG VAGAPPLFL CLLWKMLLE TDHITPIAY KILERIGAR ALSAHLRKF CDCLVFEFS NSPGGQHVN KCVDTINDM IWKYNIVTI DRLVLCLAL RTQEGSEAQ VCSFIIQLV LLKATEFRN RVQDFVKDN SPDHWNQTN WHEKHLEFH RKYPEKFAP EEQSSGYHP YFGNVCLRF LPVFDIVVH RFLEIPQVT KSLEILLEH LGCLYKFHD RPVTYLYNT LHYYETRLR DRPPLKRRL VAAVLGTLK DSLSEPYQL YLGRAADEM WTPELDYYI QVVKRVVDI LGGATANSV IDWRFNEFP NAGAHILYT SCVELMALS AGPVAVANG LLDVVAKGY VTIPSSDIH QWVNAVGLI LAALPVSYW SVLHDRLVA TLNELETWN FDCSPFRLF DFKETHAGL LHNNFSYML ALAHSVWHH AGPGQIASV PRWARECLP AVVRSEEQF LYVCHLVGP FLQRFNVAI VELTSALYE LLAQVDHAH SELKYMDPI CDLLYHIKY MFVGDSMKK ELETVVRRL RPPLQMRLR FIAHLAIEE VNAS
- >TC003334 RA
atgggcgag ccactaaca aacgtccta accgacatt ctcaaagtt gaaggcatc gaagaagcc tttagttgc tttctagtg caccgcccc agtgccgaa aaggaaaag gtggctgta tggcaacaa gagttgaac acagccctg cggagcatc ccccaggag cagctagac accgccctt cgccagtac ctcaacact gctgtacta tgttcccaa agcaagctc cagctcttg atggagctc ctggaaagt gtcgtgcgc aacggtacc attggggcc cgtatcgtg tgtgaggtg gtgatgaat ttcgataaa ctacaacac accaatgcg cggttctgg atcgagtgc tttaaactc gtgcggaaa attatagat ttggttgaa tacaagggc gttcgcgaa attatgaaa ggatgttgc gagaaggcg aaaagtctc ccctggcgc ttgaacgcg ggcttgcag ccccaaacc caggctctt ttaagcgtc gctgaacga atcatggac aggaacgcg tgcctcctg cctggctat ctcctagtg aacgagctc cagaaggcc taccctgac tgcccccac tggcgcctt gctgccctc ttctccgat tacgttggc agttttcgg gcctgtgcc caaatggtg gctatcgta ggccaggcg gaaatgcgg ccagttgtg gagcacagc ggctgtccc gaacacttg gtcaatccc tggaaactt gacccgtgc acgttgcga ttctcgctt aagggcact ttaccgtac gctcccgaa ttactagag aagcaaacc ggtttatta cgatacgtt ctggaacag ccgtacagt cgcgacatg gtgtgtggc atgttaggg ttgcagaaa cagcacaag agacactgt gccgccctt gaggaacaa ctggtcgag ttggtgatt ttggcgctg gaacgcacg gaaaatgag ggcgatgag agcgccact caagggtta tggctgcac ttgtcgtcc cagttaatt tattttgtt cttttccaa tttgcgagt ttcccaaac attgttata tcgttgcat tcgagactg caagggcgc gacttgcgg agagggcgc gaccaactc atgtgggtc ctcttgcag tttatttcc ggttcgatt cagaagaac cccctctcg aatttcctc ccagttttg cggctttac gaactgttg tttccggaa caggagccc cctttgccc gttccggac ttcaaccag ccccagtgt acgagacag atggcaatg acgtgcatt tggatccac ttgactaag aaagcgcaa gccgaaatg accaacttg acttggcca attcccgcc aaactacgc aaccatcat gatttcctc caacagttg gtgccccct aataacacc gtgttagcg atgggaaat gactacaga atcgccttg ttgtgcaac gcctactca acgaacccc gattattac agtaagcca atggcagcg ctagttgag acgatccaa gggagcccc aaatcaaca gccccacct accgcgcct ttaagcatg gccgtcttg gactcatta actgtgcat tcgaaaatg agcttaatc catagtatt gtcacacac gtgattaaa ctagcacag gccaagtta ggaatgcca ctatcacca gctttggtg gaaacgtac agtcgcctg ttggtgtac accgagatc gaatcgttg ggcatcaaa gggttcata agccagttg ttgccaacc gtatacaag tcacacgct tggggcacc ctttacaca cttctggag atgtttagt tatcggatg caccacatc cacccccac taccgtgtc cagttactc agccacttg cattcgttg gcagcggtg ccgcaagcc aaccaaacc caactgcac ttatgtgtc gaatcgacc gctttgcgt ctaatcacg gggctgggc agtggcgaa gttcagccc gaactgagc cgcttttta gccgaaccc aagacgtta atttcggcg gaatcggaa gagttgaat cgggcgctg gttttgacc ctagctagg gccacgcac gtgacgggg gccgacggc tcgtggtgt cacgagttg ctggcgacc attgcacaa agtaccccc catgcgtgg gccccccac accctcgaa tgcttcccc agggctctg gccgagttt ttcacgcaa catgccgtc cctaaagaa aacaaacaa caattgaag aaagcagtg gaggaggag taccggaag tggtcgtcc atgaacaac gaaaatgac atgatggcg cattttggg gttgcggga gcgccccca cttttcctc tgtttgttg tggaaaatg ctcctcgag actgatcac atcacgcca attgcctac aaaatcctg gagaggatt ggggcaagg gcgctttcg gcccacttg aggaagttt tgtgattgt ctcgtgttc gagtttagt aacagtcct ggggggcaa catgttaac aaatgcgtg gatacgatc aatgacatg atctggaag tacaatatt gtgacaatc gatcggttg gtcttgtgt ttggcgctg cggacgcag gaagggagc gaggctcaa gtctgttcg ttcatcatc cagttggtg ttgcttaaa gctacagag tttaggaat cgggtgcag gatttcgtc aaagacaac tcgcccgat cattggaac cagaccaac tggcacgag aaacacttg gagtttcac cggaaatac cccgagaag tttgccccc gaggaacag tcaagtggt taccacccc tattttggc aacgtgtgt ttgcgcttt ttgccggtt tttgatata gtggtgcac cggtttttg gaaataccg caagtcacc aaaagtttg gagattctg ttagaacac ttgggttgt ttgtacaag tttcatgac cgtccggtc acttatttg tacaacact ttgcactat tacgagact aggcttagg gaccgtcca cctttgaaa cgacggttg gtggctgct gtcttaggg acactcaaa gacagtctt tcggagccc taccaactg tacctcgga cgagctgct gacgagatg tggaccccc gaactggac tattacata caagtggtc aagcgagtc gttgatatt ctagggggc gccactgcc aattccgta attgattgg cggttcaat gagttccca aacgccggg gctcatatt ctttacacc agctgtgtg gaactgatg gcgttgtcc gccggtccg gtggccgta gccaacggt ttgctagac gtcgtggcc aaaggctat gtcacaatt ccctcgtca gacatccac cagtgggtc aatgctgtt ggtttaatt ctggcagcg cttccagtt agctactgg agtgttcta cacgatcga cttgtggcc acgttaaac gagctagaa acgtggaat tttgactgt tctcccttc cgtttgttc gatttcaaa gaaacccac gctgggctt ttgcataat aattttagt tatatgcta gctttggct cattcggtt tggcatcac gctggtccg gggcagatc gccagtgtg ccccgatgg gcgcgtgag tgtttaccg gctgtggta cggtccgaa gaacagttt ctctatgtt tgtcatctt gtggggccc tttttgcaa cggtttaac gtggcaata gtggaattg acgagtgct ttgtacgaa ctgttggca caagttgac cacgcgcac agcgagttg aaatatatg gacccgatc tgcgatctc ttgtaccac attaagtac atgtttgtg ggggatagc atgaagaag gagttggag actgtggtg cgccggtta agaccgcct ttgcaaatg aggttgagg tttatcgca catttggcg attgaggaa gttaatgca tcgtaa
- >TC003334 RA
agtcggcca aacataacc taccgttat tatcccatt caaataacc acaaaataa tgttttcgc ctgaaaaat aaccgtgga cacgatggg cgagccact aacaaacgt cctaaccga cattctcaa agttgaagg catcgaaga agcctttag ttgctttct agtgcaccg ccccagtgc cgaaaagga aaaggtggc tgtatggca acaagagtt gaacacagc cctgcggag catccccca ggagcagct agacaccgc ccttcgcca gtacctcaa cactgctgt actatgttc ccaaagcaa gctccagct cttgatgga gctcctgga aagtgtcgt gcgcaacgg taccattgg ggcccgtat cgtgtgtga ggtggtgat gaatttcga taaactaca acacaccaa tgcgcggtt ctggatcga gtgctttaa actcgtgcg gaaaattat agatttggt tgaatacaa gggcgttcg cgaaattat gaaaggatg ttgcgagaa ggcgaaaag tctcccctg gcgcttgaa cgcgggctt gcagcccca aacccaggc tcttttaag cgtcgctga acgaatcat ggacaggaa cgcgtgcct cctgcctgg ctatctcct agtgaacga gctccagaa ggcctaccc tgactgccc ccactggcg ccttgctgc cctcttctc cgattacgt tggcagttt tcgggcctg tgcccaaat ggtggctat cgtaggcca ggcggaaat gcggccagt tgtggagca cagcggctg tcccgaaca cttggtcaa tccctggaa acttgaccc gtgcacgtt gcgattctc gcttaaggg cactttacc gtacgctcc cgaattact agagaagca aaccggttt attacgata cgttctgga acagccgta cagtcgcga catggtgtg tggcatgtt agggttgca gaaacagca caagagaca ctgtgccgc ccttgagga acaactggt cgagttggt gattttggc gctggaacg cacggaaaa tgagggcga tgagagcgc cactcaagg gttatggct gcacttgtc gtcccagtt aatttattt tgttctttt ccaatttgc gagtttccc aaacattgt tatatcgtt gcattcgag actgcaagg gcgcgactt gcggagagg gcgcgacca actcatgtg ggtcctctt gcagtttat ttccggttc gattcagaa gaaccccct ctcgaattt cctcccagt tttgcggct ttacgaact gttgtttcc ggaacagga gcccccttt gcccgttcc ggacttcaa ccagcccca gtgtacgag acagatggc aatgacgtg catttggat ccacttgac taagaaagc gcaagccga aatgaccaa cttgacttg gccaattcc cgccaaact acgcaacca tcatgattt cctccaaca gttggtgcc ccctaataa caccgtgtt agcgatggg aaatgacta cagaatcgc cttgttgtg caacgccta ctcaacgaa ccccgatta ttacagtaa gccaatggc agcgctagt tgagacgat ccaagggag ccccaaatc aacagcccc acctaccgc gcctttaag catggccgt cttggactc attaactgt gcattcgaa aatgagctt aatccatag tattgtcac acacgtgat taaactagc acaggccaa gttaggaat gccactatc accagcttt ggtggaaac gtacagtcg cctgttggt gtacaccga gatcgaatc gttgggcat caaagggtt cataagcca gttgttgcc aaccgtata caagtcaca cgcttgggg cacccttta cacacttct ggagatgtt tagttatcg gatgcacca catccaccc ccactaccg tgtccagtt actcagcca cttgcattc gttggcagc ggtgccgca agccaacca aacccaact gcacttatg tgtcgaatc gaccgcttt gcgtctaat cacggggct gggcagtgg cgaagttca gcccgaact gagccgctt tttagccga acccaagac gttaatttc ggcggaatc ggaagagtt gaatcgggc gctggtttt gaccctagc tagggccac gcacgtgac gggggccga cggctcgtg gtgtcacga gttgctggc gaccattgc acaaagtac cccccatgc gtgggcccc ccacaccct cgaatgctt ccccagggc tctggccga gtttttcac gcaacatgc cgtccctaa agaaaacaa acaacaatt gaagaaagc agtggagga ggagtaccg gaagtggtc gtccatgaa caacgaaaa tgacatgat ggcgcattt tggggttgc gggagcgcc cccactttt cctctgttt gttgtggaa aatgctcct cgagactga tcacatcac gccaattgc ctacaaaat cctggagag gattggggc aagggcgct ttcggccca cttgaggaa gttttgtga ttgtctcgt gttcgagtt tagtaacag tcctggggg gcaacatgt taacaaatg cgtggatac gatcaatga catgatctg gaagtacaa tattgtgac aatcgatcg gttggtctt gtgtttggc gctgcggac gcaggaagg gagcgaggc tcaagtctg ttcgttcat catccagtt ggtgttgct taaagctac agagtttag gaatcgggt gcaggattt cgtcaaaga caactcgcc cgatcattg gaaccagac caactggca cgagaaaca cttggagtt tcaccggaa ataccccga gaagtttgc ccccgagga acagtcaag tggttacca cccctattt tggcaacgt gtgtttgcg ctttttgcc ggtttttga tatagtggt gcaccggtt tttggaaat accgcaagt caccaaaag tttggagat tctgttaga acacttggg ttgtttgta caagtttca tgaccgtcc ggtcactta tttgtacaa cactttgca ctattacga gactaggct tagggaccg tccaccttt gaaacgacg gttggtggc tgctgtctt agggacact caaagacag tctttcgga gccctacca actgtacct cggacgagc tgctgacga gatgtggac ccccgaact ggactatta catacaagt ggtcaagcg agtcgttga tattctagg gggcgccac tgccaattc cgtaattga ttggcggtt caatgagtt cccaaacgc cggggctca tattcttta caccagctg tgtggaact gatggcgtt gtccgccgg tccggtggc cgtagccaa cggtttgct agacgtcgt ggccaaagg ctatgtcac aattccctc gtcagacat ccaccagtg ggtcaatgc tgttggttt aattctggc agcgcttcc agttagcta ctggagtgt tctacacga tcgacttgt ggccacgtt aaacgagct agaaacgtg gaattttga ctgttctcc cttccgttt gttcgattt caaagaaac ccacgctgg gcttttgca taataattt tagttatat gctagcttt ggctcattc ggtttggca tcacgctgg tccggggca gatcgccag tgtgccccg atgggcgcg tgagtgttt accggctgt ggtacggtc cgaagaaca gtttctcta tgtttgtca tcttgtggg gcccttttt gcaacggtt taacgtggc aatagtgga attgacgag tgctttgta cgaactgtt ggcacaagt tgaccacgc gcacagcga gttgaaata tatggaccc gatctgcga tctcttgta ccacattaa gtacatgtt tgtggggga tagcatgaa gaaggagtt ggagactgt ggtgcgccg gttaagacc gcctttgca aatgaggtt gaggtttat cgcacattt ggcgattga ggaagttaa tgcatcgta actcacttg tattatgtt tttacattt acgtaa
