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Gene information
General information
TC003332 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 1365397..1368594
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003332
    iBeetle screen iB_00555

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    mature egg not present - Number of animals showing the phenotype: 3

    vitellogenic egg chamber not present - Number of animals showing the phenotype: 4
    previtellogenic egg chamber number decreased - Number of animals showing the phenotype: 4
    tropharium irregular - Number of animals showing the phenotype: 4
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 88.89% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    Pupal morphology
    wings shortened - Number of animals showing the phenotype: 3

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003332 RA
      MVMETKTTS KTSCLKALL LKAWRERWT DLQWGINIK 
      TVLPRGVSG DVYNLADCI LQQAVVGAG ANQLVLSYL 
      RHSLSAQLV SHAAVLQRL SKYNQLSKV HCVFSLLEF 
      LEGMLPGVT CCGKPEETV LATAILSIA CWLLTILLQ 
      CKGTAHLTQ KASFLLTTL MNDDFYVSM MCLARYSDP 
      ELFTETNRK CIELRASLS DSDELSKCV KKLENIDVN 
      ILSLPINSE KWPGALIQC WLEVKLVGN PGASTNSLA 
      EQLRLFQKL KGFDGPRLY AELMRGSLL ALYDVNQTA 
      HESQWGAFA FLKVPHILL ELAGKSDSN SIVSAVELM 
      LQHSPLLDA MDANSSCSS LECLLGELV KARLITEVQ 
      LKHLLEKRK PPPSVKLDT APAAAGIPK VIICAEPTL 
      AGILKTLST DYHKIQDPL LGMLHQVLT GKSFELILA 
      VATVQGQLR TLVTRLIRF NECSKSGGD KARAQLFDI 
      SFLMLVAIV QNYGANAVL DPDGESLFE QWVKSCMVE 
      RQRPKAPEQ LLRLGDPDT IEMLLQQFN AGESDFKPN 
      IKWQDVLFN MPGVMHEVL VAWEQGALV PSDVKRILD 
      AVRGRMCSL PLAAAAWLC AYMRTAPQE NLLKPVNMV 
      QQLLAPPAA EEDSLRERW QLTCEIIRK MQRDVQLPL 
      QTKSSHHLI SRQPAVEQL HSAWSGAIR RGWLDHSSA 
      KTLHCLLDT AGPRWLVSA VIQEMLKLR YRDQLQRGV 
      DLALAAFHV DIRACTLEL LSHVLPQML YNDLQADSL 
      MEPHLIALA YLTSYCVYT AFDAFSEEP EEPMAKVAR 
      LNEGEDGTL IEQLISTLR QLMTIFEDG IQEGYITQQ 
      TYFAFYLIK SLVEVKVST ASALLAAIP PALVTDLLR 
      TLPELFTYP ILLHLHDVF NTHGRNNMA KDLCILRNY 
      HLRNVSQES N
    CDS sequence
    • >TC003332 RA
      atggtgatg gaaacgaaa accacgagt aagacaagc 
      tgtctgaag gcccttttg ctgaaagcg tggcgcgag 
      cgctggact gacttacaa tggggcatt aacatcaaa 
      acggtgtta ccacgaggc gtcagtggc gatgtttac 
      aacttggct gattgcatt ctccaacaa gcggtagtc 
      ggtgccggg gccaatcag ttggttttg agttacttg 
      agacactcc ttgagcgcc cagttagtc tcacatgcg 
      gctgtgctt caacgcctc agcaaatac aaccaactc 
      agtaaagtc cactgcgtg tttagccta cttgagttc 
      ctcgaaggc atgcttccg ggtgtcact tgttgtggc 
      aaaccggaa gaaaccgtc ctggccact gcaatttta 
      tcaattgct tgttggctc ctgactatt ttattgcaa 
      tgcaaaggc accgctcat ttgactcag aaagcctcg 
      ttcttgctc accacgctc atgaatgac gatttttac 
      gtctccatg atgtgtcta gcgaggtac agcgatcct 
      gaattgttt actgagacc aaccgcaaa tgtattgag 
      ttgagggcg tcgttgtcc gattctgac gagttatct 
      aaatgcgtt aaaaaactg gaaaacatc gatgttaac 
      attttgagc ctcccgatc aatagtgag aaatggcca 
      ggtgctctg atccagtgt tggttggaa gtcaaactc 
      gtgggtaat cccggagcg tccactaat tccctagct 
      gaacagttg agactcttc caaaaattg aaaggtttt 
      gacggtccg cgcctatat gccgaattg atgagaggc 
      agtctcttg gctttatat gacgtgaat cagacggca 
      cacgaaagt caatggggg gcctttgcc tttctgaag 
      gtacctcac attctgctc gaattggcg ggcaagagt 
      gacagtaac agtattgtt agtgcagtt gagctgatg 
      ctgcagcat agcccactg ttggacgca atggatgcg 
      aacagctcg tgttcaagc ttggagtgt cttttaggc 
      gaattggtt aaagcacga ttgattact gaagtgcag 
      cttaaacat ttgttggag aagaggaaa ccaccgccg 
      tccgtcaag ctggacacg gccccagct gctgcgggg 
      attcccaaa gtgattatt tgtgcagaa cccactctt 
      gccgggatt ttgaaaaca ttatcgact gattatcac 
      aagatacag gaccccctt ttggggatg ttgcatcaa 
      gtgttgact gggaaaagc ttcgagttg attctagcc 
      gtggctacg gtgcaaggc caactgaga acgcttgtt 
      acaagactg atccgtttc aacgaatgt tccaaatca 
      gggggtgac aaagcacgg gcccaattg ttcgatata 
      agtttcctg atgttggtg gcaattgtg caaaattat 
      ggcgccaat gctgttctg gaccctgac ggggaatcg 
      ttgtttgag cagtgggtt aagtcatgt atggttgaa 
      cggcagcgg ccgaaagca cccgaacag ctccttcgt 
      ctgggtgat cctgacacg attgaaatg ctcctacaa 
      caattcaac gccggtgaa tccgatttt aaaccgaac 
      atcaaatgg caggacgtt ctcttcaat atgcccgga 
      gttatgcac gaagtgttg gtggcttgg gaacagggg 
      gctttagtg ccctcggac gtgaagcgg attttggac 
      gctgtccgt ggccgaatg tgttcgcta ccgttggct 
      gcggccgcc tggctttgc gcttacatg cggacagcc 
      ccccaagaa aacctcctc aagcccgtg aacatggtg 
      cagcaatta ctagccccc ccagcggcc gaggaagac 
      agtttgcgg gagcgctgg caactcacg tgcgagatc 
      atccgtaaa atgcaacgt gacgtccaa ttgcctctc 
      cagacgaaa agtagccac cacctgatt tcgagacaa 
      ccggctgtg gaacagtta cacagtgcc tggtctggg 
      gctattcgc aggggttgg ctggaccac agctcggct 
      aaaaccttg cactgtttg ctcgatacg gccggtccg 
      aggtggcta gtgtctgcc gttatacaa gaaatgcta 
      aagttgaga taccgcgac cagttgcag agaggtgtg 
      gatctggct ttggcggcg ttccatgtc gatatcagg 
      gcctgcact ttggagctg ctctcgcat gttttgccc 
      caaatgttg tataacgat ctccaagcg gactcgctc 
      atggagccg catttgatc gcattggct tacttgacc 
      agttattgc gtgtacacg gcgtttgat gcgttttcg 
      gaagaaccg gaagaaccg atggctaaa gttgcgcgg 
      cttaatgag ggtgaggat ggcacgctg attgagcaa 
      ctaatttcg actctcaga caactgatg acgattttt 
      gaggatggg atacaagag ggttacatc actcaacag 
      acttatttc gcgttttat ttgattaaa agtctggtt 
      gaggtgaaa gtgagcacg gcgagtgct ctcttggct 
      gcgattccg ccggctctt gtgacagac ttgcttcgg 
      actctgcct gaacttttt acttacccg attttgttg 
      catctgcac gatgtgttt aatactcac ggtcggaat 
      aacatggca aaggacttg tgtatattg cgaaattac 
      cacttgagg aacgtttcg caggagagt aattag
    mRNA sequence
    • >TC003332 RA
      ttgatttta caattttaa ttacagttt tcacgtaac 
      acattaact tgtagcgga caaattatt tctaaaatc 
      cactcaaag aattggcac ttgacaacg ttgtgctgt 
      acttgtcaa aaagctgca cttgcgtac tatttaaaa 
      aaataaatt aattggtgt ttttcccat tattaaaaa 
      tgaaaaagt aattaaata aagttatgg cacgtcaat 
      tcaagtaaa cagaactta attttgcca ttaaattga 
      ttcctctac ctcaaacca tcccacaca ccgctttgc 
      aatggtgat ggaaacgaa aaccacgag taagacaag 
      ctgtctgaa ggccctttt gctgaaagc gtggcgcga 
      gcgctggac tgacttaca atggggcat taacatcaa 
      aacggtgtt accacgagg cgtcagtgg cgatgttta 
      caacttggc tgattgcat tctccaaca agcggtagt 
      cggtgccgg ggccaatca gttggtttt gagttactt 
      gagacactc cttgagcgc ccagttagt ctcacatgc 
      ggctgtgct tcaacgcct cagcaaata caaccaact 
      cagtaaagt ccactgcgt gtttagcct acttgagtt 
      cctcgaagg catgcttcc gggtgtcac ttgttgtgg 
      caaaccgga agaaaccgt cctggccac tgcaatttt 
      atcaattgc ttgttggct cctgactat tttattgca 
      atgcaaagg caccgctca tttgactca gaaagcctc 
      gttcttgct caccacgct catgaatga cgattttta 
      cgtctccat gatgtgtct agcgaggta cagcgatcc 
      tgaattgtt tactgagac caaccgcaa atgtattga 
      gttgagggc gtcgttgtc cgattctga cgagttatc 
      taaatgcgt taaaaaact ggaaaacat cgatgttaa 
      cattttgag cctcccgat caatagtga gaaatggcc 
      aggtgctct gatccagtg ttggttgga agtcaaact 
      cgtgggtaa tcccggagc gtccactaa ttccctagc 
      tgaacagtt gagactctt ccaaaaatt gaaaggttt 
      tgacggtcc gcgcctata tgccgaatt gatgagagg 
      cagtctctt ggctttata tgacgtgaa tcagacggc 
      acacgaaag tcaatgggg ggcctttgc ctttctgaa 
      ggtacctca cattctgct cgaattggc gggcaagag 
      tgacagtaa cagtattgt tagtgcagt tgagctgat 
      gctgcagca tagcccact gttggacgc aatggatgc 
      gaacagctc gtgttcaag cttggagtg tcttttagg 
      cgaattggt taaagcacg attgattac tgaagtgca 
      gcttaaaca tttgttgga gaagaggaa accaccgcc 
      gtccgtcaa gctggacac ggccccagc tgctgcggg 
      gattcccaa agtgattat ttgtgcaga acccactct 
      tgccgggat tttgaaaac attatcgac tgattatca 
      caagataca ggaccccct tttggggat gttgcatca 
      agtgttgac tgggaaaag cttcgagtt gattctagc 
      cgtggctac ggtgcaagg ccaactgag aacgcttgt 
      tacaagact gatccgttt caacgaatg ttccaaatc 
      agggggtga caaagcacg ggcccaatt gttcgatat 
      aagtttcct gatgttggt ggcaattgt gcaaaatta 
      tggcgccaa tgctgttct ggaccctga cggggaatc 
      gttgtttga gcagtgggt taagtcatg tatggttga 
      acggcagcg gccgaaagc acccgaaca gctccttcg 
      tctgggtga tcctgacac gattgaaat gctcctaca 
      acaattcaa cgccggtga atccgattt taaaccgaa 
      catcaaatg gcaggacgt tctcttcaa tatgcccgg 
      agttatgca cgaagtgtt ggtggcttg ggaacaggg 
      ggctttagt gccctcgga cgtgaagcg gattttgga 
      cgctgtccg tggccgaat gtgttcgct accgttggc 
      tgcggccgc ctggctttg cgcttacat gcggacagc 
      cccccaaga aaacctcct caagcccgt gaacatggt 
      gcagcaatt actagcccc cccagcggc cgaggaaga 
      cagtttgcg ggagcgctg gcaactcac gtgcgagat 
      catccgtaa aatgcaacg tgacgtcca attgcctct 
      ccagacgaa aagtagcca ccacctgat ttcgagaca 
      accggctgt ggaacagtt acacagtgc ctggtctgg 
      ggctattcg caggggttg gctggacca cagctcggc 
      taaaacctt gcactgttt gctcgatac ggccggtcc 
      gaggtggct agtgtctgc cgttataca agaaatgct 
      aaagttgag ataccgcga ccagttgca gagaggtgt 
      ggatctggc tttggcggc gttccatgt cgatatcag 
      ggcctgcac tttggagct gctctcgca tgttttgcc 
      ccaaatgtt gtataacga tctccaagc ggactcgct 
      catggagcc gcatttgat cgcattggc ttacttgac 
      cagttattg cgtgtacac ggcgtttga tgcgttttc 
      ggaagaacc ggaagaacc gatggctaa agttgcgcg 
      gcttaatga gggtgagga tggcacgct gattgagca 
      actaatttc gactctcag acaactgat gacgatttt 
      tgaggatgg gatacaaga gggttacat cactcaaca 
      gacttattt cgcgtttta tttgattaa aagtctggt 
      tgaggtgaa agtgagcac ggcgagtgc tctcttggc 
      tgcgattcc gccggctct tgtgacaga cttgcttcg 
      gactctgcc tgaactttt tacttaccc gattttgtt 
      gcatctgca cgatgtgtt taatactca cggtcggaa 
      taacatggc aaaggactt gtgtatatt gcgaaatta 
      ccacttgag gaacgtttc gcaggagag taattagat 
      gtgatgact tatttaaat aaagcataa ttgaaaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY