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Gene information
General information
TC003332 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 1365397..1368594
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC003332
    iBeetle screen iB_00555

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Fertility
    mature egg not present - Number of animals showing the phenotype: 3

    vitellogenic egg chamber not present - Number of animals showing the phenotype: 4
    previtellogenic egg chamber number decreased - Number of animals showing the phenotype: 4
    tropharium irregular - Number of animals showing the phenotype: 4
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 88.89% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    pupal molt not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection

    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    Pupal morphology
    wings shortened - Number of animals showing the phenotype: 3

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003332 RA
      MVMETKTTS KTSCLKALL LKAWRERWT DLQWGINIK 
TVLPRGVSG DVYNLADCI LQQAVVGAG ANQLVLSYL 
RHSLSAQLV SHAAVLQRL SKYNQLSKV HCVFSLLEF 
LEGMLPGVT CCGKPEETV LATAILSIA CWLLTILLQ 
CKGTAHLTQ KASFLLTTL MNDDFYVSM MCLARYSDP 
ELFTETNRK CIELRASLS DSDELSKCV KKLENIDVN 
ILSLPINSE KWPGALIQC WLEVKLVGN PGASTNSLA 
EQLRLFQKL KGFDGPRLY AELMRGSLL ALYDVNQTA 
HESQWGAFA FLKVPHILL ELAGKSDSN SIVSAVELM 
LQHSPLLDA MDANSSCSS LECLLGELV KARLITEVQ 
LKHLLEKRK PPPSVKLDT APAAAGIPK VIICAEPTL 
AGILKTLST DYHKIQDPL LGMLHQVLT GKSFELILA 
VATVQGQLR TLVTRLIRF NECSKSGGD KARAQLFDI 
SFLMLVAIV QNYGANAVL DPDGESLFE QWVKSCMVE 
RQRPKAPEQ LLRLGDPDT IEMLLQQFN AGESDFKPN 
IKWQDVLFN MPGVMHEVL VAWEQGALV PSDVKRILD 
AVRGRMCSL PLAAAAWLC AYMRTAPQE NLLKPVNMV 
QQLLAPPAA EEDSLRERW QLTCEIIRK MQRDVQLPL 
QTKSSHHLI SRQPAVEQL HSAWSGAIR RGWLDHSSA 
KTLHCLLDT AGPRWLVSA VIQEMLKLR YRDQLQRGV 
DLALAAFHV DIRACTLEL LSHVLPQML YNDLQADSL 
MEPHLIALA YLTSYCVYT AFDAFSEEP EEPMAKVAR 
LNEGEDGTL IEQLISTLR QLMTIFEDG IQEGYITQQ 
TYFAFYLIK SLVEVKVST ASALLAAIP PALVTDLLR 
TLPELFTYP ILLHLHDVF NTHGRNNMA KDLCILRNY 
HLRNVSQES N
    CDS sequence
    • >TC003332 RA
      atggtgatg gaaacgaaa accacgagt aagacaagc 
tgtctgaag gcccttttg ctgaaagcg tggcgcgag 
cgctggact gacttacaa tggggcatt aacatcaaa 
acggtgtta ccacgaggc gtcagtggc gatgtttac 
aacttggct gattgcatt ctccaacaa gcggtagtc 
ggtgccggg gccaatcag ttggttttg agttacttg 
agacactcc ttgagcgcc cagttagtc tcacatgcg 
gctgtgctt caacgcctc agcaaatac aaccaactc 
agtaaagtc cactgcgtg tttagccta cttgagttc 
ctcgaaggc atgcttccg ggtgtcact tgttgtggc 
aaaccggaa gaaaccgtc ctggccact gcaatttta 
tcaattgct tgttggctc ctgactatt ttattgcaa 
tgcaaaggc accgctcat ttgactcag aaagcctcg 
ttcttgctc accacgctc atgaatgac gatttttac 
gtctccatg atgtgtcta gcgaggtac agcgatcct 
gaattgttt actgagacc aaccgcaaa tgtattgag 
ttgagggcg tcgttgtcc gattctgac gagttatct 
aaatgcgtt aaaaaactg gaaaacatc gatgttaac 
attttgagc ctcccgatc aatagtgag aaatggcca 
ggtgctctg atccagtgt tggttggaa gtcaaactc 
gtgggtaat cccggagcg tccactaat tccctagct 
gaacagttg agactcttc caaaaattg aaaggtttt 
gacggtccg cgcctatat gccgaattg atgagaggc 
agtctcttg gctttatat gacgtgaat cagacggca 
cacgaaagt caatggggg gcctttgcc tttctgaag 
gtacctcac attctgctc gaattggcg ggcaagagt 
gacagtaac agtattgtt agtgcagtt gagctgatg 
ctgcagcat agcccactg ttggacgca atggatgcg 
aacagctcg tgttcaagc ttggagtgt cttttaggc 
gaattggtt aaagcacga ttgattact gaagtgcag 
cttaaacat ttgttggag aagaggaaa ccaccgccg 
tccgtcaag ctggacacg gccccagct gctgcgggg 
attcccaaa gtgattatt tgtgcagaa cccactctt 
gccgggatt ttgaaaaca ttatcgact gattatcac 
aagatacag gaccccctt ttggggatg ttgcatcaa 
gtgttgact gggaaaagc ttcgagttg attctagcc 
gtggctacg gtgcaaggc caactgaga acgcttgtt 
acaagactg atccgtttc aacgaatgt tccaaatca 
gggggtgac aaagcacgg gcccaattg ttcgatata 
agtttcctg atgttggtg gcaattgtg caaaattat 
ggcgccaat gctgttctg gaccctgac ggggaatcg 
ttgtttgag cagtgggtt aagtcatgt atggttgaa 
cggcagcgg ccgaaagca cccgaacag ctccttcgt 
ctgggtgat cctgacacg attgaaatg ctcctacaa 
caattcaac gccggtgaa tccgatttt aaaccgaac 
atcaaatgg caggacgtt ctcttcaat atgcccgga 
gttatgcac gaagtgttg gtggcttgg gaacagggg 
gctttagtg ccctcggac gtgaagcgg attttggac 
gctgtccgt ggccgaatg tgttcgcta ccgttggct 
gcggccgcc tggctttgc gcttacatg cggacagcc 
ccccaagaa aacctcctc aagcccgtg aacatggtg 
cagcaatta ctagccccc ccagcggcc gaggaagac 
agtttgcgg gagcgctgg caactcacg tgcgagatc 
atccgtaaa atgcaacgt gacgtccaa ttgcctctc 
cagacgaaa agtagccac cacctgatt tcgagacaa 
ccggctgtg gaacagtta cacagtgcc tggtctggg 
gctattcgc aggggttgg ctggaccac agctcggct 
aaaaccttg cactgtttg ctcgatacg gccggtccg 
aggtggcta gtgtctgcc gttatacaa gaaatgcta 
aagttgaga taccgcgac cagttgcag agaggtgtg 
gatctggct ttggcggcg ttccatgtc gatatcagg 
gcctgcact ttggagctg ctctcgcat gttttgccc 
caaatgttg tataacgat ctccaagcg gactcgctc 
atggagccg catttgatc gcattggct tacttgacc 
agttattgc gtgtacacg gcgtttgat gcgttttcg 
gaagaaccg gaagaaccg atggctaaa gttgcgcgg 
cttaatgag ggtgaggat ggcacgctg attgagcaa 
ctaatttcg actctcaga caactgatg acgattttt 
gaggatggg atacaagag ggttacatc actcaacag 
acttatttc gcgttttat ttgattaaa agtctggtt 
gaggtgaaa gtgagcacg gcgagtgct ctcttggct 
gcgattccg ccggctctt gtgacagac ttgcttcgg 
actctgcct gaacttttt acttacccg attttgttg 
catctgcac gatgtgttt aatactcac ggtcggaat 
aacatggca aaggacttg tgtatattg cgaaattac 
cacttgagg aacgtttcg caggagagt aattag
    mRNA sequence
    • >TC003332 RA
      ttgatttta caattttaa ttacagttt tcacgtaac 
acattaact tgtagcgga caaattatt tctaaaatc 
cactcaaag aattggcac ttgacaacg ttgtgctgt 
acttgtcaa aaagctgca cttgcgtac tatttaaaa 
aaataaatt aattggtgt ttttcccat tattaaaaa 
tgaaaaagt aattaaata aagttatgg cacgtcaat 
tcaagtaaa cagaactta attttgcca ttaaattga 
ttcctctac ctcaaacca tcccacaca ccgctttgc 
aatggtgat ggaaacgaa aaccacgag taagacaag 
ctgtctgaa ggccctttt gctgaaagc gtggcgcga 
gcgctggac tgacttaca atggggcat taacatcaa 
aacggtgtt accacgagg cgtcagtgg cgatgttta 
caacttggc tgattgcat tctccaaca agcggtagt 
cggtgccgg ggccaatca gttggtttt gagttactt 
gagacactc cttgagcgc ccagttagt ctcacatgc 
ggctgtgct tcaacgcct cagcaaata caaccaact 
cagtaaagt ccactgcgt gtttagcct acttgagtt 
cctcgaagg catgcttcc gggtgtcac ttgttgtgg 
caaaccgga agaaaccgt cctggccac tgcaatttt 
atcaattgc ttgttggct cctgactat tttattgca 
atgcaaagg caccgctca tttgactca gaaagcctc 
gttcttgct caccacgct catgaatga cgattttta 
cgtctccat gatgtgtct agcgaggta cagcgatcc 
tgaattgtt tactgagac caaccgcaa atgtattga 
gttgagggc gtcgttgtc cgattctga cgagttatc 
taaatgcgt taaaaaact ggaaaacat cgatgttaa 
cattttgag cctcccgat caatagtga gaaatggcc 
aggtgctct gatccagtg ttggttgga agtcaaact 
cgtgggtaa tcccggagc gtccactaa ttccctagc 
tgaacagtt gagactctt ccaaaaatt gaaaggttt 
tgacggtcc gcgcctata tgccgaatt gatgagagg 
cagtctctt ggctttata tgacgtgaa tcagacggc 
acacgaaag tcaatgggg ggcctttgc ctttctgaa 
ggtacctca cattctgct cgaattggc gggcaagag 
tgacagtaa cagtattgt tagtgcagt tgagctgat 
gctgcagca tagcccact gttggacgc aatggatgc 
gaacagctc gtgttcaag cttggagtg tcttttagg 
cgaattggt taaagcacg attgattac tgaagtgca 
gcttaaaca tttgttgga gaagaggaa accaccgcc 
gtccgtcaa gctggacac ggccccagc tgctgcggg 
gattcccaa agtgattat ttgtgcaga acccactct 
tgccgggat tttgaaaac attatcgac tgattatca 
caagataca ggaccccct tttggggat gttgcatca 
agtgttgac tgggaaaag cttcgagtt gattctagc 
cgtggctac ggtgcaagg ccaactgag aacgcttgt 
tacaagact gatccgttt caacgaatg ttccaaatc 
agggggtga caaagcacg ggcccaatt gttcgatat 
aagtttcct gatgttggt ggcaattgt gcaaaatta 
tggcgccaa tgctgttct ggaccctga cggggaatc 
gttgtttga gcagtgggt taagtcatg tatggttga 
acggcagcg gccgaaagc acccgaaca gctccttcg 
tctgggtga tcctgacac gattgaaat gctcctaca 
acaattcaa cgccggtga atccgattt taaaccgaa 
catcaaatg gcaggacgt tctcttcaa tatgcccgg 
agttatgca cgaagtgtt ggtggcttg ggaacaggg 
ggctttagt gccctcgga cgtgaagcg gattttgga 
cgctgtccg tggccgaat gtgttcgct accgttggc 
tgcggccgc ctggctttg cgcttacat gcggacagc 
cccccaaga aaacctcct caagcccgt gaacatggt 
gcagcaatt actagcccc cccagcggc cgaggaaga 
cagtttgcg ggagcgctg gcaactcac gtgcgagat 
catccgtaa aatgcaacg tgacgtcca attgcctct 
ccagacgaa aagtagcca ccacctgat ttcgagaca 
accggctgt ggaacagtt acacagtgc ctggtctgg 
ggctattcg caggggttg gctggacca cagctcggc 
taaaacctt gcactgttt gctcgatac ggccggtcc 
gaggtggct agtgtctgc cgttataca agaaatgct 
aaagttgag ataccgcga ccagttgca gagaggtgt 
ggatctggc tttggcggc gttccatgt cgatatcag 
ggcctgcac tttggagct gctctcgca tgttttgcc 
ccaaatgtt gtataacga tctccaagc ggactcgct 
catggagcc gcatttgat cgcattggc ttacttgac 
cagttattg cgtgtacac ggcgtttga tgcgttttc 
ggaagaacc ggaagaacc gatggctaa agttgcgcg 
gcttaatga gggtgagga tggcacgct gattgagca 
actaatttc gactctcag acaactgat gacgatttt 
tgaggatgg gatacaaga gggttacat cactcaaca 
gacttattt cgcgtttta tttgattaa aagtctggt 
tgaggtgaa agtgagcac ggcgagtgc tctcttggc 
tgcgattcc gccggctct tgtgacaga cttgcttcg 
gactctgcc tgaactttt tacttaccc gattttgtt 
gcatctgca cgatgtgtt taatactca cggtcggaa 
taacatggc aaaggactt gtgtatatt gcgaaatta 
ccacttgag gaacgtttc gcaggagag taattagat 
gtgatgact tatttaaat aaagcataa ttgaaaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY