General information | |||
TC003332 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 1365397..1368594 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC003332 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_00555 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)vitellogenic egg chamber not present - Number of animals showing the phenotype: 4
previtellogenic egg chamber number decreased - Number of animals showing the phenotype: 4
tropharium irregular - Number of animals showing the phenotype: 4
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 88.89% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 2 (11 dpi)
pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 3 (11 dpi)
pupal molt not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (11 dpi)
pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 2 (16 dpi)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
- >TC003332 RA
MVMETKTTS KTSCLKALL LKAWRERWT DLQWGINIK TVLPRGVSG DVYNLADCI LQQAVVGAG ANQLVLSYL RHSLSAQLV SHAAVLQRL SKYNQLSKV HCVFSLLEF LEGMLPGVT CCGKPEETV LATAILSIA CWLLTILLQ CKGTAHLTQ KASFLLTTL MNDDFYVSM MCLARYSDP ELFTETNRK CIELRASLS DSDELSKCV KKLENIDVN ILSLPINSE KWPGALIQC WLEVKLVGN PGASTNSLA EQLRLFQKL KGFDGPRLY AELMRGSLL ALYDVNQTA HESQWGAFA FLKVPHILL ELAGKSDSN SIVSAVELM LQHSPLLDA MDANSSCSS LECLLGELV KARLITEVQ LKHLLEKRK PPPSVKLDT APAAAGIPK VIICAEPTL AGILKTLST DYHKIQDPL LGMLHQVLT GKSFELILA VATVQGQLR TLVTRLIRF NECSKSGGD KARAQLFDI SFLMLVAIV QNYGANAVL DPDGESLFE QWVKSCMVE RQRPKAPEQ LLRLGDPDT IEMLLQQFN AGESDFKPN IKWQDVLFN MPGVMHEVL VAWEQGALV PSDVKRILD AVRGRMCSL PLAAAAWLC AYMRTAPQE NLLKPVNMV QQLLAPPAA EEDSLRERW QLTCEIIRK MQRDVQLPL QTKSSHHLI SRQPAVEQL HSAWSGAIR RGWLDHSSA KTLHCLLDT AGPRWLVSA VIQEMLKLR YRDQLQRGV DLALAAFHV DIRACTLEL LSHVLPQML YNDLQADSL MEPHLIALA YLTSYCVYT AFDAFSEEP EEPMAKVAR LNEGEDGTL IEQLISTLR QLMTIFEDG IQEGYITQQ TYFAFYLIK SLVEVKVST ASALLAAIP PALVTDLLR TLPELFTYP ILLHLHDVF NTHGRNNMA KDLCILRNY HLRNVSQES N
- >TC003332 RA
atggtgatg gaaacgaaa accacgagt aagacaagc tgtctgaag gcccttttg ctgaaagcg tggcgcgag cgctggact gacttacaa tggggcatt aacatcaaa acggtgtta ccacgaggc gtcagtggc gatgtttac aacttggct gattgcatt ctccaacaa gcggtagtc ggtgccggg gccaatcag ttggttttg agttacttg agacactcc ttgagcgcc cagttagtc tcacatgcg gctgtgctt caacgcctc agcaaatac aaccaactc agtaaagtc cactgcgtg tttagccta cttgagttc ctcgaaggc atgcttccg ggtgtcact tgttgtggc aaaccggaa gaaaccgtc ctggccact gcaatttta tcaattgct tgttggctc ctgactatt ttattgcaa tgcaaaggc accgctcat ttgactcag aaagcctcg ttcttgctc accacgctc atgaatgac gatttttac gtctccatg atgtgtcta gcgaggtac agcgatcct gaattgttt actgagacc aaccgcaaa tgtattgag ttgagggcg tcgttgtcc gattctgac gagttatct aaatgcgtt aaaaaactg gaaaacatc gatgttaac attttgagc ctcccgatc aatagtgag aaatggcca ggtgctctg atccagtgt tggttggaa gtcaaactc gtgggtaat cccggagcg tccactaat tccctagct gaacagttg agactcttc caaaaattg aaaggtttt gacggtccg cgcctatat gccgaattg atgagaggc agtctcttg gctttatat gacgtgaat cagacggca cacgaaagt caatggggg gcctttgcc tttctgaag gtacctcac attctgctc gaattggcg ggcaagagt gacagtaac agtattgtt agtgcagtt gagctgatg ctgcagcat agcccactg ttggacgca atggatgcg aacagctcg tgttcaagc ttggagtgt cttttaggc gaattggtt aaagcacga ttgattact gaagtgcag cttaaacat ttgttggag aagaggaaa ccaccgccg tccgtcaag ctggacacg gccccagct gctgcgggg attcccaaa gtgattatt tgtgcagaa cccactctt gccgggatt ttgaaaaca ttatcgact gattatcac aagatacag gaccccctt ttggggatg ttgcatcaa gtgttgact gggaaaagc ttcgagttg attctagcc gtggctacg gtgcaaggc caactgaga acgcttgtt acaagactg atccgtttc aacgaatgt tccaaatca gggggtgac aaagcacgg gcccaattg ttcgatata agtttcctg atgttggtg gcaattgtg caaaattat ggcgccaat gctgttctg gaccctgac ggggaatcg ttgtttgag cagtgggtt aagtcatgt atggttgaa cggcagcgg ccgaaagca cccgaacag ctccttcgt ctgggtgat cctgacacg attgaaatg ctcctacaa caattcaac gccggtgaa tccgatttt aaaccgaac atcaaatgg caggacgtt ctcttcaat atgcccgga gttatgcac gaagtgttg gtggcttgg gaacagggg gctttagtg ccctcggac gtgaagcgg attttggac gctgtccgt ggccgaatg tgttcgcta ccgttggct gcggccgcc tggctttgc gcttacatg cggacagcc ccccaagaa aacctcctc aagcccgtg aacatggtg cagcaatta ctagccccc ccagcggcc gaggaagac agtttgcgg gagcgctgg caactcacg tgcgagatc atccgtaaa atgcaacgt gacgtccaa ttgcctctc cagacgaaa agtagccac cacctgatt tcgagacaa ccggctgtg gaacagtta cacagtgcc tggtctggg gctattcgc aggggttgg ctggaccac agctcggct aaaaccttg cactgtttg ctcgatacg gccggtccg aggtggcta gtgtctgcc gttatacaa gaaatgcta aagttgaga taccgcgac cagttgcag agaggtgtg gatctggct ttggcggcg ttccatgtc gatatcagg gcctgcact ttggagctg ctctcgcat gttttgccc caaatgttg tataacgat ctccaagcg gactcgctc atggagccg catttgatc gcattggct tacttgacc agttattgc gtgtacacg gcgtttgat gcgttttcg gaagaaccg gaagaaccg atggctaaa gttgcgcgg cttaatgag ggtgaggat ggcacgctg attgagcaa ctaatttcg actctcaga caactgatg acgattttt gaggatggg atacaagag ggttacatc actcaacag acttatttc gcgttttat ttgattaaa agtctggtt gaggtgaaa gtgagcacg gcgagtgct ctcttggct gcgattccg ccggctctt gtgacagac ttgcttcgg actctgcct gaacttttt acttacccg attttgttg catctgcac gatgtgttt aatactcac ggtcggaat aacatggca aaggacttg tgtatattg cgaaattac cacttgagg aacgtttcg caggagagt aattag
- >TC003332 RA
ttgatttta caattttaa ttacagttt tcacgtaac acattaact tgtagcgga caaattatt tctaaaatc cactcaaag aattggcac ttgacaacg ttgtgctgt acttgtcaa aaagctgca cttgcgtac tatttaaaa aaataaatt aattggtgt ttttcccat tattaaaaa tgaaaaagt aattaaata aagttatgg cacgtcaat tcaagtaaa cagaactta attttgcca ttaaattga ttcctctac ctcaaacca tcccacaca ccgctttgc aatggtgat ggaaacgaa aaccacgag taagacaag ctgtctgaa ggccctttt gctgaaagc gtggcgcga gcgctggac tgacttaca atggggcat taacatcaa aacggtgtt accacgagg cgtcagtgg cgatgttta caacttggc tgattgcat tctccaaca agcggtagt cggtgccgg ggccaatca gttggtttt gagttactt gagacactc cttgagcgc ccagttagt ctcacatgc ggctgtgct tcaacgcct cagcaaata caaccaact cagtaaagt ccactgcgt gtttagcct acttgagtt cctcgaagg catgcttcc gggtgtcac ttgttgtgg caaaccgga agaaaccgt cctggccac tgcaatttt atcaattgc ttgttggct cctgactat tttattgca atgcaaagg caccgctca tttgactca gaaagcctc gttcttgct caccacgct catgaatga cgattttta cgtctccat gatgtgtct agcgaggta cagcgatcc tgaattgtt tactgagac caaccgcaa atgtattga gttgagggc gtcgttgtc cgattctga cgagttatc taaatgcgt taaaaaact ggaaaacat cgatgttaa cattttgag cctcccgat caatagtga gaaatggcc aggtgctct gatccagtg ttggttgga agtcaaact cgtgggtaa tcccggagc gtccactaa ttccctagc tgaacagtt gagactctt ccaaaaatt gaaaggttt tgacggtcc gcgcctata tgccgaatt gatgagagg cagtctctt ggctttata tgacgtgaa tcagacggc acacgaaag tcaatgggg ggcctttgc ctttctgaa ggtacctca cattctgct cgaattggc gggcaagag tgacagtaa cagtattgt tagtgcagt tgagctgat gctgcagca tagcccact gttggacgc aatggatgc gaacagctc gtgttcaag cttggagtg tcttttagg cgaattggt taaagcacg attgattac tgaagtgca gcttaaaca tttgttgga gaagaggaa accaccgcc gtccgtcaa gctggacac ggccccagc tgctgcggg gattcccaa agtgattat ttgtgcaga acccactct tgccgggat tttgaaaac attatcgac tgattatca caagataca ggaccccct tttggggat gttgcatca agtgttgac tgggaaaag cttcgagtt gattctagc cgtggctac ggtgcaagg ccaactgag aacgcttgt tacaagact gatccgttt caacgaatg ttccaaatc agggggtga caaagcacg ggcccaatt gttcgatat aagtttcct gatgttggt ggcaattgt gcaaaatta tggcgccaa tgctgttct ggaccctga cggggaatc gttgtttga gcagtgggt taagtcatg tatggttga acggcagcg gccgaaagc acccgaaca gctccttcg tctgggtga tcctgacac gattgaaat gctcctaca acaattcaa cgccggtga atccgattt taaaccgaa catcaaatg gcaggacgt tctcttcaa tatgcccgg agttatgca cgaagtgtt ggtggcttg ggaacaggg ggctttagt gccctcgga cgtgaagcg gattttgga cgctgtccg tggccgaat gtgttcgct accgttggc tgcggccgc ctggctttg cgcttacat gcggacagc cccccaaga aaacctcct caagcccgt gaacatggt gcagcaatt actagcccc cccagcggc cgaggaaga cagtttgcg ggagcgctg gcaactcac gtgcgagat catccgtaa aatgcaacg tgacgtcca attgcctct ccagacgaa aagtagcca ccacctgat ttcgagaca accggctgt ggaacagtt acacagtgc ctggtctgg ggctattcg caggggttg gctggacca cagctcggc taaaacctt gcactgttt gctcgatac ggccggtcc gaggtggct agtgtctgc cgttataca agaaatgct aaagttgag ataccgcga ccagttgca gagaggtgt ggatctggc tttggcggc gttccatgt cgatatcag ggcctgcac tttggagct gctctcgca tgttttgcc ccaaatgtt gtataacga tctccaagc ggactcgct catggagcc gcatttgat cgcattggc ttacttgac cagttattg cgtgtacac ggcgtttga tgcgttttc ggaagaacc ggaagaacc gatggctaa agttgcgcg gcttaatga gggtgagga tggcacgct gattgagca actaatttc gactctcag acaactgat gacgatttt tgaggatgg gatacaaga gggttacat cactcaaca gacttattt cgcgtttta tttgattaa aagtctggt tgaggtgaa agtgagcac ggcgagtgc tctcttggc tgcgattcc gccggctct tgtgacaga cttgcttcg gactctgcc tgaactttt tacttaccc gattttgtt gcatctgca cgatgtgtt taatactca cggtcggaa taacatggc aaaggactt gtgtatatt gcgaaatta ccacttgag gaacgtttc gcaggagag taattagat gtgatgact tatttaaat aaagcataa ttgaaaaa
