General information | |||
TC003324 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 1241647..1245160 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs | TC003324 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_00553 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
remarks: muscle pattern potentially obscured by segmentation defect,
egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
potentially confused/crosscontaminated with : iB # (comma separated) 00568
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
- >TC003324 RA
MSRRHHHHN LQPYDYDRF NDQPRDRTR DQARAIEYQ DDSLSSINS DEESYLHHT NRDKKSSFY SKDKSSKNE KNSEPYHPK EPKSQPRDT KTSDSTVER LERLETMLE EFMKRSTVP APTPSVLEK GAEKPFYVE PPSVVEKKG PSDRLDKQF YLEPRRHTF TTSMWINKI QEMSFQNNW DEKTTIQYM VSQMSGTVK AWYNSLPNY DYTWPEWKL LICKVFPDN TDFVTTLKR LVTRLKQAD ESMTTYYFS KMYLIEACK ITGEDAVSC LIDGLNNQT IQIQAKAQN FLTPETLYS QFLMVLPEY SVDYAPSGV PTEATWYYS EDVPSIQSV NEDRNASQE QMFGFEADE RSEKFNYPD QANSSFEER PVKSPSNAK PPGPVHSRL DKRYIRRKD FKKQSKVPL ASKVCSICL NRGHVARNC RRKVEKVLY SRLSSSGFQ SCFYKTCTV NGQELDSFI DTGCTTVTI TEEKAHRLK LHVDFSDDR IQTYGGGSV RILGETEVL LKVDSVQAR VKAVVVPNN AQEVSVIVG QGFLNLKNV GVLLQNNTL KIYEPFCKL TD
- >TC003324 RA
atgagtcgg aggcatcac caccacaat ctccagccc tatgattac gaccgcttc aacgaccaa ccgcgggat cgaacccgg gaccaggcc agagccatc gaataccaa gacgactcc ttgagttcc ataaactca gacgaagag tcgtacctg caccacacg aatagagac aagaagtca agcttttat tcgaaggac aagtcgagc aaaaatgag aaaaacagc gagccctac cacccgaag gagcccaaa tcgcagccc cgggacacc aagacatcc gactccacg gtggagcgt ttggagcgt ctggagacc atgttggag gagttcatg aaacgtagt actgtccca gctcccaca ccaagtgtc ctcgagaaa ggggccgag aagccgttt tacgtggaa ccgcccagt gttgttgag aagaaggga cccagtgat agactcgac aagcagttt tatctggag ccgcgacga cacacgttc accacgtcc atgtggatc aacaaaatc caggagatg tcgttccag aacaactgg gatgagaag acaaccatc cagtatatg gtgtctcaa atgtccggg actgtgaaa gcgtggtac aacagcttg cccaactat gactacact tggcctgag tggaaactt ttgatttgt aaggttttc ccggacaat accgatttt gtaaccact ttgaagaga ttagtaaca cggttgaag caagctgat gagagcatg accacctac tacttcagc aaaatgtac ctgattgaa gcgtgcaaa atcactgga gaggacgca gtttcgtgt ttaatagac gggttgaac aaccaaacc atacaaata caggcaaag gctcaaaat tttctaaca cctgaaaca ctatattcg caatttttg atggtcctg ccggagtat tccgtcgac tatgcacct tctggagtt cccacggag gccacctgg tactattcg gaggacgta ccaagtatt cagtcggtt aatgaagat aggaacgct tctcaggaa caaatgttt gggtttgaa gcggatgaa aggtcagag aagtttaac tatccagat caagcgaat tcaagtttt gaggaacgt ccggtaaag tcgccctca aatgcaaaa cctccaggt cctgtacac tcaagactt gataaaagg tacatcaga cggaaagat ttcaaaaag cagagtaag gttccattg gcttccaaa gtgtgttcc atttgcttg aatcggggc cacgttgcg aggaattgt agaagaaaa gtggagaaa gtgttatat tcccgtttg tccagctcg ggcttccaa agttgtttt tataaaact tgtacggtt aatggacag gagttggat tcttttatc gataccgga tgcacgact gtgacaatt actgaggaa aaagcacac aggttgaaa ctacacgtg gattttagt gacgatagg attcaaaca tacggcgga ggatcagtt cgtattttg ggggaaacc gaagtttta ctgaaggtg gattcagtt caggctcgt gtcaaggcg gtagttgta ccaaataat gcacaagag gtttcggtc atcgttgga caaggattc ctaaacttg aagaatgta ggcgtgtta ctgcaaaat aacacgcta aaaatttat gagccattc tgtaaacta acagattaa
- >TC003324 RA
tcaaaataa aaattgatt tttttggta aattttcgt ttgcggttg gcagttttg gaatttaat tctcagttg cccaaatgt caatttgat gtgtatttg tgagtggca aaatccttt tttatgtaa taacacgaa caattgcgt tggaaaatg agtcggagg catcaccac cacaatctc cagccctat gattacgac cgcttcaac gaccaaccg cgggatcga acccgggac caggccaga gccatcgaa taccaagac gactccttg agttccata aactcagac gaagagtcg tacctgcac cacacgaat agagacaag aagtcaagc ttttattcg aaggacaag tcgagcaaa aatgagaaa aacagcgag ccctaccac ccgaaggag cccaaatcg cagccccgg gacaccaag acatccgac tccacggtg gagcgtttg gagcgtctg gagaccatg ttggaggag ttcatgaaa cgtagtact gtcccagct cccacacca agtgtcctc gagaaaggg gccgagaag ccgttttac gtggaaccg cccagtgtt gttgagaag aagggaccc agtgataga ctcgacaag cagttttat ctggagccg cgacgacac acgttcacc acgtccatg tggatcaac aaaatccag gagatgtcg ttccagaac aactgggat gagaagaca accatccag tatatggtg tctcaaatg tccgggact gtgaaagcg tggtacaac agcttgccc aactatgac tacacttgg cctgagtgg aaacttttg atttgtaag gttttcccg gacaatacc gattttgta accactttg aagagatta gtaacacgg ttgaagcaa gctgatgag agcatgacc acctactac ttcagcaaa atgtacctg attgaagcg tgcaaaatc actggagag gacgcagtt tcgtgttta atagacggg ttgaacaac caaaccata caaatacag gcaaaggct caaaatttt ctaacacct gaaacacta tattcgcaa tttttgatg gtcctgccg gagtattcc gtcgactat gcaccttct ggagttccc acggaggcc acctggtac tattcggag gacgtacca agtattcag tcggttaat gaagatagg aacgcttct caggaacaa atgtttggg tttgaagcg gatgaaagg tcagagaag tttaactat ccagatcaa gcgaattca agttttgag gaacgtccg gtaaagtcg ccctcaaat gcaaaacct ccaggtcct gtacactca agacttgat aaaaggtac atcagacgg aaagatttc aaaaagcag agtaaggtt ccattggct tccaaagtg tgttccatt tgcttgaat cggggccac gttgcgagg aattgtaga agaaaagtg gagaaagtg ttatattcc cgtttgtcc agctcgggc ttccaaagt tgtttttat aaaacttgt acggttaat ggacaggag ttggattct tttatcgat accggatgc acgactgtg acaattact gaggaaaaa gcacacagg ttgaaacta cacgtggat tttagtgac gataggatt caaacatac ggcggagga tcagttcgt attttgggg gaaaccgaa gttttactg aaggtggat tcagttcag gctcgtgtc aaggcggta gttgtacca aataatgca caagaggtt tcggtcatc gttggacaa ggattccta aacttgaag aatgtaggc gtgttactg caaaataac acgctaaaa atttatgag ccattctgt aaactaaca gattaactt ttgatttgg tgtatggta agtgtaaaa aagtagcag ttgtttttt attgaacaa aaggctggc gatgtttta aatgtggta aaacaaatt gcaaggatt ctatttttg taaacttga attatttgc caatggaat tggacaaca aataataca aaagtggtt tgactggtt tgaactgta gtgttttac taattcgac aagaagatt tggagtttt tactgaaag gaaccgata ttaaaaacg aggacttgg acccattcg gattttttt gttcttgct gggacagct gtcgatcgg atagaaatt tctaccttt ttatgtttt ttttttcat tgtcatcgt tggatttct aaataaatg tttcacaat ttgtgaatc tgtgaaacc actgatctg gtcctggat ctttaacgt aactgaata tcctgatta aacttttta gaaactttt attaccgaa gcggtattt tacttagtt cagaagtgg gattttttc aatttggaa gtctatgga tttaaacga agacgatac ttttggtct tcatgtaag aagaaggaa actttggca atttattgc ggaatgagg aagcgaaaa acatggtgc tgtaatcgc atggactat gacgttatc tggacatta tttttccat gagactaag caaaaagtc tcaaacaat ctatattat tgataatga tatttgcat aaagtcaac aaataagag acctggcga attctgaaa aggtttttt cctactgac tgaagtctt ctgcaaagg tcgtttcat aagatactg gaactgtta ctgatttgg tggttgaag gaaatttgg ctgtgttat attttacat tgctcgtaa tcgcgaaat gagattatt tattatcat tactgagaa aaacgtgca ctctggagg caatcaagc aacttgaag agttatgat ttatgaatt ttatgatgg attgtgttt ttctattag tgggtgatc tctaaacaa tctggctta tttgtaccg tttttaaaa cttcactaa taattaaat aattgtgtt cttcggtta acacaacat aattgatag ttttagaat taaattcgg tattaatac tataaaaat agttgattt ttgctgtga taaaaacag ttttaatac aaatttgtt ccttaaagt gttgtaaag ttcaacatc gaacaaaat tatgtatgt gaatgtgtg cgtgtgtga atgttttgt gataaagaa ataaataaa ataaaaagt gtgtgactg ttctaataa atgagtgtg ttaaaattt ttgtgttgc attttttta gttccgtgt ttaaataat gacaatgat taaaaacag tgcttccga caattttaa attaattta tatttgaag tgtttgcgc ggatctctc aatcattac ggaatcacc attaattcc actgtgtgt gtattggaa aaacaaagt acaaaaagt gtagtgtta acggtgatt tcgtaatta ttgcatatt ttgttttga ttccttgtt ttaaatatt aattttctg ttcatagat tccaaccag tgggacgcg tgcttcagg tgtcaccca gtttaagtt accgtttgt tttttttag aattagaaa tttatgttt cttacttaa atttaagtt taaacgcct ttgtaattg aaaataaag tgttcatac tcca
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
