Status
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Gene information
General information
TC003324 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 1241647..1245160
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC003324
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
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      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
      {{ blurReferenceResult }}
      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_00553

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Adult morphology
      potentially confused/crosscontaminated with : iB # (comma separated) 00568
      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      remarks: muscle pattern potentially obscured by segmentation defect,
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
      egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%

      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
      potentially confused/crosscontaminated with : iB # (comma separated) 00568
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC003324 RA
        MSRRHHHHN LQPYDYDRF NDQPRDRTR DQARAIEYQ 
        DDSLSSINS DEESYLHHT NRDKKSSFY SKDKSSKNE 
        KNSEPYHPK EPKSQPRDT KTSDSTVER LERLETMLE 
        EFMKRSTVP APTPSVLEK GAEKPFYVE PPSVVEKKG 
        PSDRLDKQF YLEPRRHTF TTSMWINKI QEMSFQNNW 
        DEKTTIQYM VSQMSGTVK AWYNSLPNY DYTWPEWKL 
        LICKVFPDN TDFVTTLKR LVTRLKQAD ESMTTYYFS 
        KMYLIEACK ITGEDAVSC LIDGLNNQT IQIQAKAQN 
        FLTPETLYS QFLMVLPEY SVDYAPSGV PTEATWYYS 
        EDVPSIQSV NEDRNASQE QMFGFEADE RSEKFNYPD 
        QANSSFEER PVKSPSNAK PPGPVHSRL DKRYIRRKD 
        FKKQSKVPL ASKVCSICL NRGHVARNC RRKVEKVLY 
        SRLSSSGFQ SCFYKTCTV NGQELDSFI DTGCTTVTI 
        TEEKAHRLK LHVDFSDDR IQTYGGGSV RILGETEVL 
        LKVDSVQAR VKAVVVPNN AQEVSVIVG QGFLNLKNV 
        GVLLQNNTL KIYEPFCKL TD
      CDS sequence
      • >TC003324 RA
        atgagtcgg aggcatcac caccacaat ctccagccc 
        tatgattac gaccgcttc aacgaccaa ccgcgggat 
        cgaacccgg gaccaggcc agagccatc gaataccaa 
        gacgactcc ttgagttcc ataaactca gacgaagag 
        tcgtacctg caccacacg aatagagac aagaagtca 
        agcttttat tcgaaggac aagtcgagc aaaaatgag 
        aaaaacagc gagccctac cacccgaag gagcccaaa 
        tcgcagccc cgggacacc aagacatcc gactccacg 
        gtggagcgt ttggagcgt ctggagacc atgttggag 
        gagttcatg aaacgtagt actgtccca gctcccaca 
        ccaagtgtc ctcgagaaa ggggccgag aagccgttt 
        tacgtggaa ccgcccagt gttgttgag aagaaggga 
        cccagtgat agactcgac aagcagttt tatctggag 
        ccgcgacga cacacgttc accacgtcc atgtggatc 
        aacaaaatc caggagatg tcgttccag aacaactgg 
        gatgagaag acaaccatc cagtatatg gtgtctcaa 
        atgtccggg actgtgaaa gcgtggtac aacagcttg 
        cccaactat gactacact tggcctgag tggaaactt 
        ttgatttgt aaggttttc ccggacaat accgatttt 
        gtaaccact ttgaagaga ttagtaaca cggttgaag 
        caagctgat gagagcatg accacctac tacttcagc 
        aaaatgtac ctgattgaa gcgtgcaaa atcactgga 
        gaggacgca gtttcgtgt ttaatagac gggttgaac 
        aaccaaacc atacaaata caggcaaag gctcaaaat 
        tttctaaca cctgaaaca ctatattcg caatttttg 
        atggtcctg ccggagtat tccgtcgac tatgcacct 
        tctggagtt cccacggag gccacctgg tactattcg 
        gaggacgta ccaagtatt cagtcggtt aatgaagat 
        aggaacgct tctcaggaa caaatgttt gggtttgaa 
        gcggatgaa aggtcagag aagtttaac tatccagat 
        caagcgaat tcaagtttt gaggaacgt ccggtaaag 
        tcgccctca aatgcaaaa cctccaggt cctgtacac 
        tcaagactt gataaaagg tacatcaga cggaaagat 
        ttcaaaaag cagagtaag gttccattg gcttccaaa 
        gtgtgttcc atttgcttg aatcggggc cacgttgcg 
        aggaattgt agaagaaaa gtggagaaa gtgttatat 
        tcccgtttg tccagctcg ggcttccaa agttgtttt 
        tataaaact tgtacggtt aatggacag gagttggat 
        tcttttatc gataccgga tgcacgact gtgacaatt 
        actgaggaa aaagcacac aggttgaaa ctacacgtg 
        gattttagt gacgatagg attcaaaca tacggcgga 
        ggatcagtt cgtattttg ggggaaacc gaagtttta 
        ctgaaggtg gattcagtt caggctcgt gtcaaggcg 
        gtagttgta ccaaataat gcacaagag gtttcggtc 
        atcgttgga caaggattc ctaaacttg aagaatgta 
        ggcgtgtta ctgcaaaat aacacgcta aaaatttat 
        gagccattc tgtaaacta acagattaa
      mRNA sequence
      • >TC003324 RA
        tcaaaataa aaattgatt tttttggta aattttcgt 
        ttgcggttg gcagttttg gaatttaat tctcagttg 
        cccaaatgt caatttgat gtgtatttg tgagtggca 
        aaatccttt tttatgtaa taacacgaa caattgcgt 
        tggaaaatg agtcggagg catcaccac cacaatctc 
        cagccctat gattacgac cgcttcaac gaccaaccg 
        cgggatcga acccgggac caggccaga gccatcgaa 
        taccaagac gactccttg agttccata aactcagac 
        gaagagtcg tacctgcac cacacgaat agagacaag 
        aagtcaagc ttttattcg aaggacaag tcgagcaaa 
        aatgagaaa aacagcgag ccctaccac ccgaaggag 
        cccaaatcg cagccccgg gacaccaag acatccgac 
        tccacggtg gagcgtttg gagcgtctg gagaccatg 
        ttggaggag ttcatgaaa cgtagtact gtcccagct 
        cccacacca agtgtcctc gagaaaggg gccgagaag 
        ccgttttac gtggaaccg cccagtgtt gttgagaag 
        aagggaccc agtgataga ctcgacaag cagttttat 
        ctggagccg cgacgacac acgttcacc acgtccatg 
        tggatcaac aaaatccag gagatgtcg ttccagaac 
        aactgggat gagaagaca accatccag tatatggtg 
        tctcaaatg tccgggact gtgaaagcg tggtacaac 
        agcttgccc aactatgac tacacttgg cctgagtgg 
        aaacttttg atttgtaag gttttcccg gacaatacc 
        gattttgta accactttg aagagatta gtaacacgg 
        ttgaagcaa gctgatgag agcatgacc acctactac 
        ttcagcaaa atgtacctg attgaagcg tgcaaaatc 
        actggagag gacgcagtt tcgtgttta atagacggg 
        ttgaacaac caaaccata caaatacag gcaaaggct 
        caaaatttt ctaacacct gaaacacta tattcgcaa 
        tttttgatg gtcctgccg gagtattcc gtcgactat 
        gcaccttct ggagttccc acggaggcc acctggtac 
        tattcggag gacgtacca agtattcag tcggttaat 
        gaagatagg aacgcttct caggaacaa atgtttggg 
        tttgaagcg gatgaaagg tcagagaag tttaactat 
        ccagatcaa gcgaattca agttttgag gaacgtccg 
        gtaaagtcg ccctcaaat gcaaaacct ccaggtcct 
        gtacactca agacttgat aaaaggtac atcagacgg 
        aaagatttc aaaaagcag agtaaggtt ccattggct 
        tccaaagtg tgttccatt tgcttgaat cggggccac 
        gttgcgagg aattgtaga agaaaagtg gagaaagtg 
        ttatattcc cgtttgtcc agctcgggc ttccaaagt 
        tgtttttat aaaacttgt acggttaat ggacaggag 
        ttggattct tttatcgat accggatgc acgactgtg 
        acaattact gaggaaaaa gcacacagg ttgaaacta 
        cacgtggat tttagtgac gataggatt caaacatac 
        ggcggagga tcagttcgt attttgggg gaaaccgaa 
        gttttactg aaggtggat tcagttcag gctcgtgtc 
        aaggcggta gttgtacca aataatgca caagaggtt 
        tcggtcatc gttggacaa ggattccta aacttgaag 
        aatgtaggc gtgttactg caaaataac acgctaaaa 
        atttatgag ccattctgt aaactaaca gattaactt 
        ttgatttgg tgtatggta agtgtaaaa aagtagcag 
        ttgtttttt attgaacaa aaggctggc gatgtttta 
        aatgtggta aaacaaatt gcaaggatt ctatttttg 
        taaacttga attatttgc caatggaat tggacaaca 
        aataataca aaagtggtt tgactggtt tgaactgta 
        gtgttttac taattcgac aagaagatt tggagtttt 
        tactgaaag gaaccgata ttaaaaacg aggacttgg 
        acccattcg gattttttt gttcttgct gggacagct 
        gtcgatcgg atagaaatt tctaccttt ttatgtttt 
        ttttttcat tgtcatcgt tggatttct aaataaatg 
        tttcacaat ttgtgaatc tgtgaaacc actgatctg 
        gtcctggat ctttaacgt aactgaata tcctgatta 
        aacttttta gaaactttt attaccgaa gcggtattt 
        tacttagtt cagaagtgg gattttttc aatttggaa 
        gtctatgga tttaaacga agacgatac ttttggtct 
        tcatgtaag aagaaggaa actttggca atttattgc 
        ggaatgagg aagcgaaaa acatggtgc tgtaatcgc 
        atggactat gacgttatc tggacatta tttttccat 
        gagactaag caaaaagtc tcaaacaat ctatattat 
        tgataatga tatttgcat aaagtcaac aaataagag 
        acctggcga attctgaaa aggtttttt cctactgac 
        tgaagtctt ctgcaaagg tcgtttcat aagatactg 
        gaactgtta ctgatttgg tggttgaag gaaatttgg 
        ctgtgttat attttacat tgctcgtaa tcgcgaaat 
        gagattatt tattatcat tactgagaa aaacgtgca 
        ctctggagg caatcaagc aacttgaag agttatgat 
        ttatgaatt ttatgatgg attgtgttt ttctattag 
        tgggtgatc tctaaacaa tctggctta tttgtaccg 
        tttttaaaa cttcactaa taattaaat aattgtgtt 
        cttcggtta acacaacat aattgatag ttttagaat 
        taaattcgg tattaatac tataaaaat agttgattt 
        ttgctgtga taaaaacag ttttaatac aaatttgtt 
        ccttaaagt gttgtaaag ttcaacatc gaacaaaat 
        tatgtatgt gaatgtgtg cgtgtgtga atgttttgt 
        gataaagaa ataaataaa ataaaaagt gtgtgactg 
        ttctaataa atgagtgtg ttaaaattt ttgtgttgc 
        attttttta gttccgtgt ttaaataat gacaatgat 
        taaaaacag tgcttccga caattttaa attaattta 
        tatttgaag tgtttgcgc ggatctctc aatcattac 
        ggaatcacc attaattcc actgtgtgt gtattggaa 
        aaacaaagt acaaaaagt gtagtgtta acggtgatt 
        tcgtaatta ttgcatatt ttgttttga ttccttgtt 
        ttaaatatt aattttctg ttcatagat tccaaccag 
        tgggacgcg tgcttcagg tgtcaccca gtttaagtt 
        accgtttgt tttttttag aattagaaa tttatgttt 
        cttacttaa atttaagtt taaacgcct ttgtaattg 
        aaaataaag tgttcatac tcca
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY