Status
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Gene information
General information
TC003324 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 1241647..1245160
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      		TC003324
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes

      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
      {{ blurEvidenceResult }}
      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
      {{ blurReferenceResult }}
      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
      If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_00553

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection

      Adult morphology
      potentially confused/crosscontaminated with : iB # (comma separated) 00568
      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      remarks: muscle pattern potentially obscured by segmentation defect,
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
       egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%

      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
      potentially confused/crosscontaminated with : iB # (comma separated) 00568
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
      Days post injection

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC003324 RA
        MSRRHHHHN LQPYDYDRF NDQPRDRTR DQARAIEYQ 
DDSLSSINS DEESYLHHT NRDKKSSFY SKDKSSKNE 
KNSEPYHPK EPKSQPRDT KTSDSTVER LERLETMLE 
EFMKRSTVP APTPSVLEK GAEKPFYVE PPSVVEKKG 
PSDRLDKQF YLEPRRHTF TTSMWINKI QEMSFQNNW 
DEKTTIQYM VSQMSGTVK AWYNSLPNY DYTWPEWKL 
LICKVFPDN TDFVTTLKR LVTRLKQAD ESMTTYYFS 
KMYLIEACK ITGEDAVSC LIDGLNNQT IQIQAKAQN 
FLTPETLYS QFLMVLPEY SVDYAPSGV PTEATWYYS 
EDVPSIQSV NEDRNASQE QMFGFEADE RSEKFNYPD 
QANSSFEER PVKSPSNAK PPGPVHSRL DKRYIRRKD 
FKKQSKVPL ASKVCSICL NRGHVARNC RRKVEKVLY 
SRLSSSGFQ SCFYKTCTV NGQELDSFI DTGCTTVTI 
TEEKAHRLK LHVDFSDDR IQTYGGGSV RILGETEVL 
LKVDSVQAR VKAVVVPNN AQEVSVIVG QGFLNLKNV 
GVLLQNNTL KIYEPFCKL TD
      CDS sequence
      • >TC003324 RA
        atgagtcgg aggcatcac caccacaat ctccagccc 
tatgattac gaccgcttc aacgaccaa ccgcgggat 
cgaacccgg gaccaggcc agagccatc gaataccaa 
gacgactcc ttgagttcc ataaactca gacgaagag 
tcgtacctg caccacacg aatagagac aagaagtca 
agcttttat tcgaaggac aagtcgagc aaaaatgag 
aaaaacagc gagccctac cacccgaag gagcccaaa 
tcgcagccc cgggacacc aagacatcc gactccacg 
gtggagcgt ttggagcgt ctggagacc atgttggag 
gagttcatg aaacgtagt actgtccca gctcccaca 
ccaagtgtc ctcgagaaa ggggccgag aagccgttt 
tacgtggaa ccgcccagt gttgttgag aagaaggga 
cccagtgat agactcgac aagcagttt tatctggag 
ccgcgacga cacacgttc accacgtcc atgtggatc 
aacaaaatc caggagatg tcgttccag aacaactgg 
gatgagaag acaaccatc cagtatatg gtgtctcaa 
atgtccggg actgtgaaa gcgtggtac aacagcttg 
cccaactat gactacact tggcctgag tggaaactt 
ttgatttgt aaggttttc ccggacaat accgatttt 
gtaaccact ttgaagaga ttagtaaca cggttgaag 
caagctgat gagagcatg accacctac tacttcagc 
aaaatgtac ctgattgaa gcgtgcaaa atcactgga 
gaggacgca gtttcgtgt ttaatagac gggttgaac 
aaccaaacc atacaaata caggcaaag gctcaaaat 
tttctaaca cctgaaaca ctatattcg caatttttg 
atggtcctg ccggagtat tccgtcgac tatgcacct 
tctggagtt cccacggag gccacctgg tactattcg 
gaggacgta ccaagtatt cagtcggtt aatgaagat 
aggaacgct tctcaggaa caaatgttt gggtttgaa 
gcggatgaa aggtcagag aagtttaac tatccagat 
caagcgaat tcaagtttt gaggaacgt ccggtaaag 
tcgccctca aatgcaaaa cctccaggt cctgtacac 
tcaagactt gataaaagg tacatcaga cggaaagat 
ttcaaaaag cagagtaag gttccattg gcttccaaa 
gtgtgttcc atttgcttg aatcggggc cacgttgcg 
aggaattgt agaagaaaa gtggagaaa gtgttatat 
tcccgtttg tccagctcg ggcttccaa agttgtttt 
tataaaact tgtacggtt aatggacag gagttggat 
tcttttatc gataccgga tgcacgact gtgacaatt 
actgaggaa aaagcacac aggttgaaa ctacacgtg 
gattttagt gacgatagg attcaaaca tacggcgga 
ggatcagtt cgtattttg ggggaaacc gaagtttta 
ctgaaggtg gattcagtt caggctcgt gtcaaggcg 
gtagttgta ccaaataat gcacaagag gtttcggtc 
atcgttgga caaggattc ctaaacttg aagaatgta 
ggcgtgtta ctgcaaaat aacacgcta aaaatttat 
gagccattc tgtaaacta acagattaa
      mRNA sequence
      • >TC003324 RA
        tcaaaataa aaattgatt tttttggta aattttcgt 
ttgcggttg gcagttttg gaatttaat tctcagttg 
cccaaatgt caatttgat gtgtatttg tgagtggca 
aaatccttt tttatgtaa taacacgaa caattgcgt 
tggaaaatg agtcggagg catcaccac cacaatctc 
cagccctat gattacgac cgcttcaac gaccaaccg 
cgggatcga acccgggac caggccaga gccatcgaa 
taccaagac gactccttg agttccata aactcagac 
gaagagtcg tacctgcac cacacgaat agagacaag 
aagtcaagc ttttattcg aaggacaag tcgagcaaa 
aatgagaaa aacagcgag ccctaccac ccgaaggag 
cccaaatcg cagccccgg gacaccaag acatccgac 
tccacggtg gagcgtttg gagcgtctg gagaccatg 
ttggaggag ttcatgaaa cgtagtact gtcccagct 
cccacacca agtgtcctc gagaaaggg gccgagaag 
ccgttttac gtggaaccg cccagtgtt gttgagaag 
aagggaccc agtgataga ctcgacaag cagttttat 
ctggagccg cgacgacac acgttcacc acgtccatg 
tggatcaac aaaatccag gagatgtcg ttccagaac 
aactgggat gagaagaca accatccag tatatggtg 
tctcaaatg tccgggact gtgaaagcg tggtacaac 
agcttgccc aactatgac tacacttgg cctgagtgg 
aaacttttg atttgtaag gttttcccg gacaatacc 
gattttgta accactttg aagagatta gtaacacgg 
ttgaagcaa gctgatgag agcatgacc acctactac 
ttcagcaaa atgtacctg attgaagcg tgcaaaatc 
actggagag gacgcagtt tcgtgttta atagacggg 
ttgaacaac caaaccata caaatacag gcaaaggct 
caaaatttt ctaacacct gaaacacta tattcgcaa 
tttttgatg gtcctgccg gagtattcc gtcgactat 
gcaccttct ggagttccc acggaggcc acctggtac 
tattcggag gacgtacca agtattcag tcggttaat 
gaagatagg aacgcttct caggaacaa atgtttggg 
tttgaagcg gatgaaagg tcagagaag tttaactat 
ccagatcaa gcgaattca agttttgag gaacgtccg 
gtaaagtcg ccctcaaat gcaaaacct ccaggtcct 
gtacactca agacttgat aaaaggtac atcagacgg 
aaagatttc aaaaagcag agtaaggtt ccattggct 
tccaaagtg tgttccatt tgcttgaat cggggccac 
gttgcgagg aattgtaga agaaaagtg gagaaagtg 
ttatattcc cgtttgtcc agctcgggc ttccaaagt 
tgtttttat aaaacttgt acggttaat ggacaggag 
ttggattct tttatcgat accggatgc acgactgtg 
acaattact gaggaaaaa gcacacagg ttgaaacta 
cacgtggat tttagtgac gataggatt caaacatac 
ggcggagga tcagttcgt attttgggg gaaaccgaa 
gttttactg aaggtggat tcagttcag gctcgtgtc 
aaggcggta gttgtacca aataatgca caagaggtt 
tcggtcatc gttggacaa ggattccta aacttgaag 
aatgtaggc gtgttactg caaaataac acgctaaaa 
atttatgag ccattctgt aaactaaca gattaactt 
ttgatttgg tgtatggta agtgtaaaa aagtagcag 
ttgtttttt attgaacaa aaggctggc gatgtttta 
aatgtggta aaacaaatt gcaaggatt ctatttttg 
taaacttga attatttgc caatggaat tggacaaca 
aataataca aaagtggtt tgactggtt tgaactgta 
gtgttttac taattcgac aagaagatt tggagtttt 
tactgaaag gaaccgata ttaaaaacg aggacttgg 
acccattcg gattttttt gttcttgct gggacagct 
gtcgatcgg atagaaatt tctaccttt ttatgtttt 
ttttttcat tgtcatcgt tggatttct aaataaatg 
tttcacaat ttgtgaatc tgtgaaacc actgatctg 
gtcctggat ctttaacgt aactgaata tcctgatta 
aacttttta gaaactttt attaccgaa gcggtattt 
tacttagtt cagaagtgg gattttttc aatttggaa 
gtctatgga tttaaacga agacgatac ttttggtct 
tcatgtaag aagaaggaa actttggca atttattgc 
ggaatgagg aagcgaaaa acatggtgc tgtaatcgc 
atggactat gacgttatc tggacatta tttttccat 
gagactaag caaaaagtc tcaaacaat ctatattat 
tgataatga tatttgcat aaagtcaac aaataagag 
acctggcga attctgaaa aggtttttt cctactgac 
tgaagtctt ctgcaaagg tcgtttcat aagatactg 
gaactgtta ctgatttgg tggttgaag gaaatttgg 
ctgtgttat attttacat tgctcgtaa tcgcgaaat 
gagattatt tattatcat tactgagaa aaacgtgca 
ctctggagg caatcaagc aacttgaag agttatgat 
ttatgaatt ttatgatgg attgtgttt ttctattag 
tgggtgatc tctaaacaa tctggctta tttgtaccg 
tttttaaaa cttcactaa taattaaat aattgtgtt 
cttcggtta acacaacat aattgatag ttttagaat 
taaattcgg tattaatac tataaaaat agttgattt 
ttgctgtga taaaaacag ttttaatac aaatttgtt 
ccttaaagt gttgtaaag ttcaacatc gaacaaaat 
tatgtatgt gaatgtgtg cgtgtgtga atgttttgt 
gataaagaa ataaataaa ataaaaagt gtgtgactg 
ttctaataa atgagtgtg ttaaaattt ttgtgttgc 
attttttta gttccgtgt ttaaataat gacaatgat 
taaaaacag tgcttccga caattttaa attaattta 
tatttgaag tgtttgcgc ggatctctc aatcattac 
ggaatcacc attaattcc actgtgtgt gtattggaa 
aaacaaagt acaaaaagt gtagtgtta acggtgatt 
tcgtaatta ttgcatatt ttgttttga ttccttgtt 
ttaaatatt aattttctg ttcatagat tccaaccag 
tgggacgcg tgcttcagg tgtcaccca gtttaagtt 
accgtttgt tttttttag aattagaaa tttatgttt 
cttacttaa atttaagtt taaacgcct ttgtaattg 
aaaataaag tgttcatac tcca

      If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
      Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY