Status
Please wait ...
Gene information
General information
TC003310 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 1059912..1074745
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003310
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00551

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: 4 - Number of animals showing the phenotype: 3
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    thorax partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen constricted - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    Pupal morphology
    oblique dorsal thoracic muscles thinner - Number of animals showing the phenotype: 10

    oblique dorsal thoracic muscles partially fragmented - Number of animals showing the phenotype: 10
    Adult morphology
    motility decreased - (19 dpi)
    Days post injection


    Fertility
    egg production: number <50% of expected -
    ovary without detectable defects - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003310 RA
      MALGAAVNN SEPKTNNNL DGDKVGKTT PGDAKIAVV 
      NGELPLQNG HVDVSPEKQ TKDAEESEK KDEENKNKD 
      EVVFIQDVG FSIKILSPG AEPFDIQVS SMELVQEIH 
      QLLMDREDT CHRTCFSLQ LDGNTLDNF AELKNIEGL 
      KEGSVIKVV EEPYTMREA RIHVRHVRD LLKSLDPAD 
      AYNGIECNS LSFLNTVTQ GDILEKKKM RPESVDCTP 
      PDFIMPDSK DRPLTALQP QLKDQKVAQ CVKVLTTSA 
      WNPPPGYRK IHGDLMYLY VVTLEDKHY YISACSRGF 
      YINQSTAEE FNPKPATPS HLCHSLIEL LNQISPQFK 
      RNFALLQKK RSQRHPFER VATPYQLYS WTAPMMEHT 
      IDAIRAEDT FSSKLGYEE HIPGQTRDW NEELQTTRE 
      LPRKTLPER LLRERAIFK VHSDFVAAA TRGAMAVID 
      GNVMAINPG EEAKMQMFI WNNIFFSLG FDVRDHYKE 
      LGGDAAAFV APRNDLQGV RVYSAVDLP GLYTLGTVV 
      IDYRGYRVT AQSIIPGIL EREQEQSVV YGSIDFGKT 
      VLSHPKYLD LLNKAGQQL KILPHHVLN DKDEPVELC 
      SSVECKGII GNDGRHYIL DLLRTFPPD VNFLQLEDE 
      ELSKEVKLL GFPIKHKHK LCCLRQELI DSFVDSRYM 
      MFIKYAAYH LQQLNIRKN DAKINNNQA IEDNKTENE 
      ENKKSENDD GKKEQIEDE EAKKIVESI TDGTNKIEL 
      EESTKNIVK TAAVAVGSL KDTEFDIRF NPDVYSPGI 
      KHCDNLDPT ISKQRQLVK DAAEFLPTV QIPTFIRDC 
      LDHSSAPMD GFTLSEAMH NRGINIRYL GKVTNLLAK 
      VKQLEYLHS IAVAELLLR AAKHIFTVY LQGSEMMNL 
      AVAIAHFLN CFLYSGQVP NPLQGVDES KNNRKRNKR 
      RGKLNPLAC NDNNEWANL TPKSLWSQL KQELKSYFD 
      YDLSTNDID ATTETFCLQ KISLLRGFC LKTGIQILL 
      RDYNFDSKN KLIFYEEDI LNIFPIVKH INPRATDAY 
      NFYTTGQNK IQQGYLKDG YELISEALN LLNNVYGVM 
      HPEIAQCLR MIARLNYIM GEHTEAMAY QQKAVLMSE 
      RVNGIDHPY TITEYAHLA LYCFANSQV STALKLLYR 
      ARYLAVLVC GENHPEVAL LDSNISLIL HAVGEYELS 
      LRFLEKALA LNIKYYGAK SLKVAVTYH LVARTQSCM 
      GNFRCALTN EKEAYAIYK QQLGENHEK TKESSECLK 
      HLTQQAVVL QKKMNEIYT GKNGASLPP IQIQPPSMS 
      SVLDMLNVI NGILFVQIS QEDIENFKA EIEKRQLNE 
      ENNEADEEE KAKPEALEN
    CDS sequence
    • >TC003310 RA
      atggctctt ggtgcagca gtgaacaat tcggaacca 
      aagaccaac aataacctc gacggcgac aaagtcggc 
      aaaaccacg ccaggggat gcgaaaatt gcagtcgta 
      aatggagaa ttaccacta caaaacggc cacgtggac 
      gtcagtcca gaaaaacaa accaaagac gccgaagaa 
      tcggagaaa aaagacgaa gaaaataaa aacaaagac 
      gaagtcgtc ttcatccaa gatgtaggt ttttcaatc 
      aaaatccta agtcccgga gctgaaccc ttcgacatc 
      caagtctcc agtatggag ctcgtccaa gaaatccac 
      cagttgttg atggaccgt gaagacacg tgtcaccga 
      acgtgtttc tcgctccaa ttggacggc aatacgctt 
      gataatttc gccgaattg aaaaacatc gaaggcttg 
      aaagagggt tcagttatc aaagtcgtg gaagaacct 
      tacaccatg cgtgaagca agaattcac gtgagacat 
      gtgagagat ttactcaaa tctctcgat ccggccgat 
      gcctacaat gggattgag tgcaattct ttgagtttc 
      cttaatacg gtcactcag ggcgatatt ttggagaag 
      aaaaaaatg cgtcccgaa agtgttgac tgtactcct 
      cctgatttt ataatgccg gatagtaaa gaccgaccg 
      ttgacggcc cttcagcca caattgaaa gaccaaaaa 
      gtggcgcag tgtgttaaa gttttgacc acttcggct 
      tggaatcca ccaccgggg tataggaag atccacggt 
      gatctgatg tatctttat gttgtcact ttggaggat 
      aaacactac tacatatcg gcctgttca cgtggtttt 
      tatattaat caatctaca gctgaagaa tttaaccct 
      aaaccggca actcctagt catttgtgc cattcttta 
      attgagctt cttaaccaa atttcgcca caattcaaa 
      cgtaatttt gctctactc cagaagaag aggagtcaa 
      agacacccg tttgaacgt gtggcaact ccctatcaa 
      ctttatagt tggactgca cccatgatg gagcatacc 
      atagatgcc ataagagct gaagacact ttttcctca 
      aaactgggc tacgaggag cacatcccc ggccaaact 
      cgcgactgg aacgaagaa cttcaaacc acaagagaa 
      ctcccgcgt aaaaccctc cctgaacgt ttgttacgc 
      gaacgtgca atttttaaa gtacatagc gattttgtt 
      gcggcagca acacgtggt gcaatggcc gttattgac 
      ggtaacgta atggcaata aatccaggc gaagaagcc 
      aaaatgcaa atgttcatt tggaacaac atctttttc 
      tcgcttggt tttgacgtt cgagatcat tacaaagag 
      ttgggcggc gatgctgca gcgtttgtt gcacctcga 
      aacgattta caaggcgtt cgtgtttac agtgctgtt 
      gatttacca gggctgtat actttgggt actgttgtt 
      attgattat agggggtat agagttaca gcacagtcg 
      attattccg gggattctt gaacgtgaa caggaacaa 
      agtgttgtt tatgggtcg attgatttc gggaaaact 
      gtgctaagt caccccaag tatctagat ttgttgaat 
      aaagcaggc caacaattg aaaatctta cctcatcat 
      gtacttaac gataaggat gaaccagtt gaactgtgc 
      tcaagtgtt gaatgtaaa gggattata ggtaacgac 
      ggacggcac tatattttg gatttgtta cgtactttc 
      ccacctgat gttaatttc ttgcaactt gaagacgaa 
      gaattgagc aaagaggtg aaactgttg ggttttcca 
      attaaacac aaacacaaa ttgtgttgt ttgcgccaa 
      gaattgatc gatagtttt gtcgattcg cgttacatg 
      atgttcatc aagtatgcc gcatatcat ttgcaacag 
      ttgaatata cgtaaaaac gatgctaaa atcaataac 
      aatcaagcg attgaagac aataagact gaaaacgag 
      gaaaacaaa aagagtgaa aatgatgac ggcaagaag 
      gaacaaatt gaagatgaa gaagcaaag aagattgtc 
      gagagtata actgatgga accaacaaa atcgaactg 
      gaagagagt accaaaaac attgttaag actgcagct 
      gtggctgtt ggctccctc aaagacacg gaatttgat 
      attcgtttc aatccagat gtttactca ccgggtatc 
      aaacattgt gataatcta gacccgacc atttcgaag 
      caaagacag ttggttaaa gacgctgct gaatttttg 
      cccaccgtg caaattccc acttttatt cgcgattgt 
      ttagatcat tcttcggcc ccaatggac ggttttact 
      ttatccgag gcgatgcat aatcgcgga attaacatt 
      agatatctc ggtaaagtc acaaatttg ttagcaaaa 
      gtaaaacag ttggaatat ttgcatagc attgctgtt 
      gcggaattg ctgctaagg gccgccaaa catatcttt 
      accgtttat ttgcagggg tctgaaatg atgaatttg 
      gctgttgcg attgctcac ttcctcaac tgtttccta 
      tattctggc caagttccc aatccattg caaggagtt 
      gatgagagc aaaaacaac aggaagcgc aacaaacgt 
      cgaggtaaa ttgaatccg ttggcttgt aacgacaat 
      aacgaatgg gcgaatctc accccaaaa tcgctttgg 
      tcgcaatta aaacaagaa ttgaaatct tatttcgat 
      tatgatttg tctaccaac gatattgat gcaaccacc 
      gaaacattc tgtttacaa aaaatctca ttattgcga 
      ggtttttgt ctaaagacc ggaatccag attttattg 
      agggattat aatttcgat tcaaagaat aaacttatt 
      ttctacgaa gaagatatt ctgaatatt ttcccaatc 
      gtcaaacac attaatccg agagcaact gatgcttat 
      aatttctat acaacggga cagaataag atacaacaa 
      gggtatctt aaagatggg tacgagtta attagtgaa 
      gccttgaat ttgttgaat aatgtgtat ggggtgatg 
      catccggaa attgcacaa tgtttgaga atgattgct 
      cgacttaat tatatcatg ggagagcac actgaagct 
      atggcctat cagcagaag gctgttttg atgtcggaa 
      agggtcaac ggaatcgac catccctac acaattacc 
      gaatatgca catctcgcc ttatattgc ttcgctaat 
      agtcaagtt agtaccgcc ctgaaattg ttatacagg 
      gcgagatat cttgccgta ctcgtgtgc ggtgagaat 
      catccagaa gttgccttg cttgatagc aatatcagt 
      ttaattctt cacgccgtt ggcgaatac gaactttct 
      ttgcgtttc ttagaaaag gccttagct ttaaatatc 
      aaatattac ggggccaaa tctcttaaa gtcgccgtc 
      acttatcat ctagtggcg aggacccaa agttgcatg 
      ggtaacttc cggtgtgcg ttaaccaat gaaaaggaa 
      gcttacgcc atctataaa caacagctc ggggaaaat 
      cacgaaaag accaaagaa tcgtcagag tgtctcaaa 
      cacctgaca cagcaagcc gttgtgttg cagaaaaaa 
      atgaacgag atctatacg gggaaaaat ggagcttca 
      ctgccacct atacaaatc cagccgcct tccatgagt 
      tcggtcttg gacatgttg aatgtcatt aatgggatt 
      ttgttcgtt cagataagt caagaggat atcgagaat 
      tttaaagcc gaaatcgag aaaagacaa ctgaacgaa 
      gagaataat gaagcggat gaggaagag aaagcaaaa 
      ccagaagct ttggaaaat tag
    mRNA sequence
    • >TC003310 RA
      tagatctag tgctttaag gggggggtt attttcgaa 
      gcacgtgct aaacaaaat gttcctgtc gttgtaaaa 
      tatcgtatc gttggacat tttttaaat tagaaacgc 
      gtctattcc tcgctaatt gaaacaata acgactgtg 
      tgataaaca ttcaacgtg ggtacaaaa tggctcttg 
      gtgcagcag tgaacaatt cggaaccaa agaccaaca 
      ataacctcg acggcgaca aagtcggca aaaccacgc 
      caggggatg cgaaaattg cagtcgtaa atggagaat 
      taccactac aaaacggcc acgtggacg tcagtccag 
      aaaaacaaa ccaaagacg ccgaagaat cggagaaaa 
      aagacgaag aaaataaaa acaaagacg aagtcgtct 
      tcatccaag atgtaggtt tttcaatca aaatcctaa 
      gtcccggag ctgaaccct tcgacatcc aagtctcca 
      gtatggagc tcgtccaag aaatccacc agttgttga 
      tggaccgtg aagacacgt gtcaccgaa cgtgtttct 
      cgctccaat tggacggca atacgcttg ataatttcg 
      ccgaattga aaaacatcg aaggcttga aagagggtt 
      cagttatca aagtcgtgg aagaacctt acaccatgc 
      gtgaagcaa gaattcacg tgagacatg tgagagatt 
      tactcaaat ctctcgatc cggccgatg cctacaatg 
      ggattgagt gcaattctt tgagtttcc ttaatacgg 
      tcactcagg gcgatattt tggagaaga aaaaaatgc 
      gtcccgaaa gtgttgact gtactcctc ctgatttta 
      taatgccgg atagtaaag accgaccgt tgacggccc 
      ttcagccac aattgaaag accaaaaag tggcgcagt 
      gtgttaaag ttttgacca cttcggctt ggaatccac 
      caccggggt ataggaaga tccacggtg atctgatgt 
      atctttatg ttgtcactt tggaggata aacactact 
      acatatcgg cctgttcac gtggttttt atattaatc 
      aatctacag ctgaagaat ttaacccta aaccggcaa 
      ctcctagtc atttgtgcc attctttaa ttgagcttc 
      ttaaccaaa tttcgccac aattcaaac gtaattttg 
      ctctactcc agaagaaga ggagtcaaa gacacccgt 
      ttgaacgtg tggcaactc cctatcaac tttatagtt 
      ggactgcac ccatgatgg agcatacca tagatgcca 
      taagagctg aagacactt tttcctcaa aactgggct 
      acgaggagc acatccccg gccaaactc gcgactgga 
      acgaagaac ttcaaacca caagagaac tcccgcgta 
      aaaccctcc ctgaacgtt tgttacgcg aacgtgcaa 
      tttttaaag tacatagcg attttgttg cggcagcaa 
      cacgtggtg caatggccg ttattgacg gtaacgtaa 
      tggcaataa atccaggcg aagaagcca aaatgcaaa 
      tgttcattt ggaacaaca tctttttct cgcttggtt 
      ttgacgttc gagatcatt acaaagagt tgggcggcg 
      atgctgcag cgtttgttg cacctcgaa acgatttac 
      aaggcgttc gtgtttaca gtgctgttg atttaccag 
      ggctgtata ctttgggta ctgttgtta ttgattata 
      gggggtata gagttacag cacagtcga ttattccgg 
      ggattcttg aacgtgaac aggaacaaa gtgttgttt 
      atgggtcga ttgatttcg ggaaaactg tgctaagtc 
      accccaagt atctagatt tgttgaata aagcaggcc 
      aacaattga aaatcttac ctcatcatg tacttaacg 
      ataaggatg aaccagttg aactgtgct caagtgttg 
      aatgtaaag ggattatag gtaacgacg gacggcact 
      atattttgg atttgttac gtactttcc cacctgatg 
      ttaatttct tgcaacttg aagacgaag aattgagca 
      aagaggtga aactgttgg gttttccaa ttaaacaca 
      aacacaaat tgtgttgtt tgcgccaag aattgatcg 
      atagttttg tcgattcgc gttacatga tgttcatca 
      agtatgccg catatcatt tgcaacagt tgaatatac 
      gtaaaaacg atgctaaaa tcaataaca atcaagcga 
      ttgaagaca ataagactg aaaacgagg aaaacaaaa 
      agagtgaaa atgatgacg gcaagaagg aacaaattg 
      aagatgaag aagcaaaga agattgtcg agagtataa 
      ctgatggaa ccaacaaaa tcgaactgg aagagagta 
      ccaaaaaca ttgttaaga ctgcagctg tggctgttg 
      gctccctca aagacacgg aatttgata ttcgtttca 
      atccagatg tttactcac cgggtatca aacattgtg 
      ataatctag acccgacca tttcgaagc aaagacagt 
      tggttaaag acgctgctg aatttttgc ccaccgtgc 
      aaattccca cttttattc gcgattgtt tagatcatt 
      cttcggccc caatggacg gttttactt tatccgagg 
      cgatgcata atcgcggaa ttaacatta gatatctcg 
      gtaaagtca caaatttgt tagcaaaag taaaacagt 
      tggaatatt tgcatagca ttgctgttg cggaattgc 
      tgctaaggg ccgccaaac atatcttta ccgtttatt 
      tgcaggggt ctgaaatga tgaatttgg ctgttgcga 
      ttgctcact tcctcaact gtttcctat attctggcc 
      aagttccca atccattgc aaggagttg atgagagca 
      aaaacaaca ggaagcgca acaaacgtc gaggtaaat 
      tgaatccgt tggcttgta acgacaata acgaatggg 
      cgaatctca ccccaaaat cgctttggt cgcaattaa 
      aacaagaat tgaaatctt atttcgatt atgatttgt 
      ctaccaacg atattgatg caaccaccg aaacattct 
      gtttacaaa aaatctcat tattgcgag gtttttgtc 
      taaagaccg gaatccaga ttttattga gggattata 
      atttcgatt caaagaata aacttattt tctacgaag 
      aagatattc tgaatattt tcccaatcg tcaaacaca 
      ttaatccga gagcaactg atgcttata atttctata 
      caacgggac agaataaga tacaacaag ggtatctta 
      aagatgggt acgagttaa ttagtgaag ccttgaatt 
      tgttgaata atgtgtatg gggtgatgc atccggaaa 
      ttgcacaat gtttgagaa tgattgctc gacttaatt 
      atatcatgg gagagcaca ctgaagcta tggcctatc 
      agcagaagg ctgttttga tgtcggaaa gggtcaacg 
      gaatcgacc atccctaca caattaccg aatatgcac 
      atctcgcct tatattgct tcgctaata gtcaagtta 
      gtaccgccc tgaaattgt tatacaggg cgagatatc 
      ttgccgtac tcgtgtgcg gtgagaatc atccagaag 
      ttgccttgc ttgatagca atatcagtt taattcttc 
      acgccgttg gcgaatacg aactttctt tgcgtttct 
      tagaaaagg ccttagctt taaatatca aatattacg 
      gggccaaat ctcttaaag tcgccgtca cttatcatc 
      tagtggcga ggacccaaa gttgcatgg gtaacttcc 
      ggtgtgcgt taaccaatg aaaaggaag cttacgcca 
      tctataaac aacagctcg gggaaaatc acgaaaaga 
      ccaaagaat cgtcagagt gtctcaaac acctgacac 
      agcaagccg ttgtgttgc agaaaaaaa tgaacgaga 
      tctatacgg ggaaaaatg gagcttcac tgccaccta 
      tacaaatcc agccgcctt ccatgagtt cggtcttgg 
      acatgttga atgtcatta atgggattt tgttcgttc 
      agataagtc aagaggata tcgagaatt ttaaagccg 
      aaatcgaga aaagacaac tgaacgaag agaataatg 
      aagcggatg aggaagaga aagcaaaac cagaagctt 
      tggaaaatt agtaggatt tttagatgt attgaaaag 
      gatagtcgt ctcgttagt taatttcgt agtggttct 
      tgtgcgtgc attttccaa cgagtctgt aagtaacat 
      gcattttta tcgctcaat gtaatacta gtcaaaagt 
      aagaaactg ccttccatt gtaataaaa tgttgttga 
      cgattttaa gtgatctag tgttgtgga tttctttcc 
      cgagtactg ctattgttg ttgttgttg taagttaaa 
      tatgaaata tgctgttgt tttgtgata cgcattaat 
      taatcgtag aaagttgtg attaaatag ccaaggatg 
      gtataaatt tgcatgttg cctggacaa gggaaatta 
      ttttttatt gtattttac tttgagtgc gccaacttt 
      agtaaggtg cagttttct atgatagaa cagattttt 
      ttgtaatct aaattttgt taaaataaa tccagttga 
      ttaca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY