Status
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Gene information
General information
TC003310 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 1059912..1074745
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC003310
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00551

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: 4 - Number of animals showing the phenotype: 3
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
     thorax partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen constricted - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    Pupal morphology
    oblique dorsal thoracic muscles thinner - Number of animals showing the phenotype: 10

    oblique dorsal thoracic muscles partially fragmented - Number of animals showing the phenotype: 10
    Adult morphology
    motility decreased - (19 dpi)
    Days post injection

    Fertility
    egg production: number <50% of expected -
    ovary without detectable defects - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003310 RA
      MALGAAVNN SEPKTNNNL DGDKVGKTT PGDAKIAVV 
NGELPLQNG HVDVSPEKQ TKDAEESEK KDEENKNKD 
EVVFIQDVG FSIKILSPG AEPFDIQVS SMELVQEIH 
QLLMDREDT CHRTCFSLQ LDGNTLDNF AELKNIEGL 
KEGSVIKVV EEPYTMREA RIHVRHVRD LLKSLDPAD 
AYNGIECNS LSFLNTVTQ GDILEKKKM RPESVDCTP 
PDFIMPDSK DRPLTALQP QLKDQKVAQ CVKVLTTSA 
WNPPPGYRK IHGDLMYLY VVTLEDKHY YISACSRGF 
YINQSTAEE FNPKPATPS HLCHSLIEL LNQISPQFK 
RNFALLQKK RSQRHPFER VATPYQLYS WTAPMMEHT 
IDAIRAEDT FSSKLGYEE HIPGQTRDW NEELQTTRE 
LPRKTLPER LLRERAIFK VHSDFVAAA TRGAMAVID 
GNVMAINPG EEAKMQMFI WNNIFFSLG FDVRDHYKE 
LGGDAAAFV APRNDLQGV RVYSAVDLP GLYTLGTVV 
IDYRGYRVT AQSIIPGIL EREQEQSVV YGSIDFGKT 
VLSHPKYLD LLNKAGQQL KILPHHVLN DKDEPVELC 
SSVECKGII GNDGRHYIL DLLRTFPPD VNFLQLEDE 
ELSKEVKLL GFPIKHKHK LCCLRQELI DSFVDSRYM 
MFIKYAAYH LQQLNIRKN DAKINNNQA IEDNKTENE 
ENKKSENDD GKKEQIEDE EAKKIVESI TDGTNKIEL 
EESTKNIVK TAAVAVGSL KDTEFDIRF NPDVYSPGI 
KHCDNLDPT ISKQRQLVK DAAEFLPTV QIPTFIRDC 
LDHSSAPMD GFTLSEAMH NRGINIRYL GKVTNLLAK 
VKQLEYLHS IAVAELLLR AAKHIFTVY LQGSEMMNL 
AVAIAHFLN CFLYSGQVP NPLQGVDES KNNRKRNKR 
RGKLNPLAC NDNNEWANL TPKSLWSQL KQELKSYFD 
YDLSTNDID ATTETFCLQ KISLLRGFC LKTGIQILL 
RDYNFDSKN KLIFYEEDI LNIFPIVKH INPRATDAY 
NFYTTGQNK IQQGYLKDG YELISEALN LLNNVYGVM 
HPEIAQCLR MIARLNYIM GEHTEAMAY QQKAVLMSE 
RVNGIDHPY TITEYAHLA LYCFANSQV STALKLLYR 
ARYLAVLVC GENHPEVAL LDSNISLIL HAVGEYELS 
LRFLEKALA LNIKYYGAK SLKVAVTYH LVARTQSCM 
GNFRCALTN EKEAYAIYK QQLGENHEK TKESSECLK 
HLTQQAVVL QKKMNEIYT GKNGASLPP IQIQPPSMS 
SVLDMLNVI NGILFVQIS QEDIENFKA EIEKRQLNE 
ENNEADEEE KAKPEALEN
    CDS sequence
    • >TC003310 RA
      atggctctt ggtgcagca gtgaacaat tcggaacca 
aagaccaac aataacctc gacggcgac aaagtcggc 
aaaaccacg ccaggggat gcgaaaatt gcagtcgta 
aatggagaa ttaccacta caaaacggc cacgtggac 
gtcagtcca gaaaaacaa accaaagac gccgaagaa 
tcggagaaa aaagacgaa gaaaataaa aacaaagac 
gaagtcgtc ttcatccaa gatgtaggt ttttcaatc 
aaaatccta agtcccgga gctgaaccc ttcgacatc 
caagtctcc agtatggag ctcgtccaa gaaatccac 
cagttgttg atggaccgt gaagacacg tgtcaccga 
acgtgtttc tcgctccaa ttggacggc aatacgctt 
gataatttc gccgaattg aaaaacatc gaaggcttg 
aaagagggt tcagttatc aaagtcgtg gaagaacct 
tacaccatg cgtgaagca agaattcac gtgagacat 
gtgagagat ttactcaaa tctctcgat ccggccgat 
gcctacaat gggattgag tgcaattct ttgagtttc 
cttaatacg gtcactcag ggcgatatt ttggagaag 
aaaaaaatg cgtcccgaa agtgttgac tgtactcct 
cctgatttt ataatgccg gatagtaaa gaccgaccg 
ttgacggcc cttcagcca caattgaaa gaccaaaaa 
gtggcgcag tgtgttaaa gttttgacc acttcggct 
tggaatcca ccaccgggg tataggaag atccacggt 
gatctgatg tatctttat gttgtcact ttggaggat 
aaacactac tacatatcg gcctgttca cgtggtttt 
tatattaat caatctaca gctgaagaa tttaaccct 
aaaccggca actcctagt catttgtgc cattcttta 
attgagctt cttaaccaa atttcgcca caattcaaa 
cgtaatttt gctctactc cagaagaag aggagtcaa 
agacacccg tttgaacgt gtggcaact ccctatcaa 
ctttatagt tggactgca cccatgatg gagcatacc 
atagatgcc ataagagct gaagacact ttttcctca 
aaactgggc tacgaggag cacatcccc ggccaaact 
cgcgactgg aacgaagaa cttcaaacc acaagagaa 
ctcccgcgt aaaaccctc cctgaacgt ttgttacgc 
gaacgtgca atttttaaa gtacatagc gattttgtt 
gcggcagca acacgtggt gcaatggcc gttattgac 
ggtaacgta atggcaata aatccaggc gaagaagcc 
aaaatgcaa atgttcatt tggaacaac atctttttc 
tcgcttggt tttgacgtt cgagatcat tacaaagag 
ttgggcggc gatgctgca gcgtttgtt gcacctcga 
aacgattta caaggcgtt cgtgtttac agtgctgtt 
gatttacca gggctgtat actttgggt actgttgtt 
attgattat agggggtat agagttaca gcacagtcg 
attattccg gggattctt gaacgtgaa caggaacaa 
agtgttgtt tatgggtcg attgatttc gggaaaact 
gtgctaagt caccccaag tatctagat ttgttgaat 
aaagcaggc caacaattg aaaatctta cctcatcat 
gtacttaac gataaggat gaaccagtt gaactgtgc 
tcaagtgtt gaatgtaaa gggattata ggtaacgac 
ggacggcac tatattttg gatttgtta cgtactttc 
ccacctgat gttaatttc ttgcaactt gaagacgaa 
gaattgagc aaagaggtg aaactgttg ggttttcca 
attaaacac aaacacaaa ttgtgttgt ttgcgccaa 
gaattgatc gatagtttt gtcgattcg cgttacatg 
atgttcatc aagtatgcc gcatatcat ttgcaacag 
ttgaatata cgtaaaaac gatgctaaa atcaataac 
aatcaagcg attgaagac aataagact gaaaacgag 
gaaaacaaa aagagtgaa aatgatgac ggcaagaag 
gaacaaatt gaagatgaa gaagcaaag aagattgtc 
gagagtata actgatgga accaacaaa atcgaactg 
gaagagagt accaaaaac attgttaag actgcagct 
gtggctgtt ggctccctc aaagacacg gaatttgat 
attcgtttc aatccagat gtttactca ccgggtatc 
aaacattgt gataatcta gacccgacc atttcgaag 
caaagacag ttggttaaa gacgctgct gaatttttg 
cccaccgtg caaattccc acttttatt cgcgattgt 
ttagatcat tcttcggcc ccaatggac ggttttact 
ttatccgag gcgatgcat aatcgcgga attaacatt 
agatatctc ggtaaagtc acaaatttg ttagcaaaa 
gtaaaacag ttggaatat ttgcatagc attgctgtt 
gcggaattg ctgctaagg gccgccaaa catatcttt 
accgtttat ttgcagggg tctgaaatg atgaatttg 
gctgttgcg attgctcac ttcctcaac tgtttccta 
tattctggc caagttccc aatccattg caaggagtt 
gatgagagc aaaaacaac aggaagcgc aacaaacgt 
cgaggtaaa ttgaatccg ttggcttgt aacgacaat 
aacgaatgg gcgaatctc accccaaaa tcgctttgg 
tcgcaatta aaacaagaa ttgaaatct tatttcgat 
tatgatttg tctaccaac gatattgat gcaaccacc 
gaaacattc tgtttacaa aaaatctca ttattgcga 
ggtttttgt ctaaagacc ggaatccag attttattg 
agggattat aatttcgat tcaaagaat aaacttatt 
ttctacgaa gaagatatt ctgaatatt ttcccaatc 
gtcaaacac attaatccg agagcaact gatgcttat 
aatttctat acaacggga cagaataag atacaacaa 
gggtatctt aaagatggg tacgagtta attagtgaa 
gccttgaat ttgttgaat aatgtgtat ggggtgatg 
catccggaa attgcacaa tgtttgaga atgattgct 
cgacttaat tatatcatg ggagagcac actgaagct 
atggcctat cagcagaag gctgttttg atgtcggaa 
agggtcaac ggaatcgac catccctac acaattacc 
gaatatgca catctcgcc ttatattgc ttcgctaat 
agtcaagtt agtaccgcc ctgaaattg ttatacagg 
gcgagatat cttgccgta ctcgtgtgc ggtgagaat 
catccagaa gttgccttg cttgatagc aatatcagt 
ttaattctt cacgccgtt ggcgaatac gaactttct 
ttgcgtttc ttagaaaag gccttagct ttaaatatc 
aaatattac ggggccaaa tctcttaaa gtcgccgtc 
acttatcat ctagtggcg aggacccaa agttgcatg 
ggtaacttc cggtgtgcg ttaaccaat gaaaaggaa 
gcttacgcc atctataaa caacagctc ggggaaaat 
cacgaaaag accaaagaa tcgtcagag tgtctcaaa 
cacctgaca cagcaagcc gttgtgttg cagaaaaaa 
atgaacgag atctatacg gggaaaaat ggagcttca 
ctgccacct atacaaatc cagccgcct tccatgagt 
tcggtcttg gacatgttg aatgtcatt aatgggatt 
ttgttcgtt cagataagt caagaggat atcgagaat 
tttaaagcc gaaatcgag aaaagacaa ctgaacgaa 
gagaataat gaagcggat gaggaagag aaagcaaaa 
ccagaagct ttggaaaat tag
    mRNA sequence
    • >TC003310 RA
      tagatctag tgctttaag gggggggtt attttcgaa 
gcacgtgct aaacaaaat gttcctgtc gttgtaaaa 
tatcgtatc gttggacat tttttaaat tagaaacgc 
gtctattcc tcgctaatt gaaacaata acgactgtg 
tgataaaca ttcaacgtg ggtacaaaa tggctcttg 
gtgcagcag tgaacaatt cggaaccaa agaccaaca 
ataacctcg acggcgaca aagtcggca aaaccacgc 
caggggatg cgaaaattg cagtcgtaa atggagaat 
taccactac aaaacggcc acgtggacg tcagtccag 
aaaaacaaa ccaaagacg ccgaagaat cggagaaaa 
aagacgaag aaaataaaa acaaagacg aagtcgtct 
tcatccaag atgtaggtt tttcaatca aaatcctaa 
gtcccggag ctgaaccct tcgacatcc aagtctcca 
gtatggagc tcgtccaag aaatccacc agttgttga 
tggaccgtg aagacacgt gtcaccgaa cgtgtttct 
cgctccaat tggacggca atacgcttg ataatttcg 
ccgaattga aaaacatcg aaggcttga aagagggtt 
cagttatca aagtcgtgg aagaacctt acaccatgc 
gtgaagcaa gaattcacg tgagacatg tgagagatt 
tactcaaat ctctcgatc cggccgatg cctacaatg 
ggattgagt gcaattctt tgagtttcc ttaatacgg 
tcactcagg gcgatattt tggagaaga aaaaaatgc 
gtcccgaaa gtgttgact gtactcctc ctgatttta 
taatgccgg atagtaaag accgaccgt tgacggccc 
ttcagccac aattgaaag accaaaaag tggcgcagt 
gtgttaaag ttttgacca cttcggctt ggaatccac 
caccggggt ataggaaga tccacggtg atctgatgt 
atctttatg ttgtcactt tggaggata aacactact 
acatatcgg cctgttcac gtggttttt atattaatc 
aatctacag ctgaagaat ttaacccta aaccggcaa 
ctcctagtc atttgtgcc attctttaa ttgagcttc 
ttaaccaaa tttcgccac aattcaaac gtaattttg 
ctctactcc agaagaaga ggagtcaaa gacacccgt 
ttgaacgtg tggcaactc cctatcaac tttatagtt 
ggactgcac ccatgatgg agcatacca tagatgcca 
taagagctg aagacactt tttcctcaa aactgggct 
acgaggagc acatccccg gccaaactc gcgactgga 
acgaagaac ttcaaacca caagagaac tcccgcgta 
aaaccctcc ctgaacgtt tgttacgcg aacgtgcaa 
tttttaaag tacatagcg attttgttg cggcagcaa 
cacgtggtg caatggccg ttattgacg gtaacgtaa 
tggcaataa atccaggcg aagaagcca aaatgcaaa 
tgttcattt ggaacaaca tctttttct cgcttggtt 
ttgacgttc gagatcatt acaaagagt tgggcggcg 
atgctgcag cgtttgttg cacctcgaa acgatttac 
aaggcgttc gtgtttaca gtgctgttg atttaccag 
ggctgtata ctttgggta ctgttgtta ttgattata 
gggggtata gagttacag cacagtcga ttattccgg 
ggattcttg aacgtgaac aggaacaaa gtgttgttt 
atgggtcga ttgatttcg ggaaaactg tgctaagtc 
accccaagt atctagatt tgttgaata aagcaggcc 
aacaattga aaatcttac ctcatcatg tacttaacg 
ataaggatg aaccagttg aactgtgct caagtgttg 
aatgtaaag ggattatag gtaacgacg gacggcact 
atattttgg atttgttac gtactttcc cacctgatg 
ttaatttct tgcaacttg aagacgaag aattgagca 
aagaggtga aactgttgg gttttccaa ttaaacaca 
aacacaaat tgtgttgtt tgcgccaag aattgatcg 
atagttttg tcgattcgc gttacatga tgttcatca 
agtatgccg catatcatt tgcaacagt tgaatatac 
gtaaaaacg atgctaaaa tcaataaca atcaagcga 
ttgaagaca ataagactg aaaacgagg aaaacaaaa 
agagtgaaa atgatgacg gcaagaagg aacaaattg 
aagatgaag aagcaaaga agattgtcg agagtataa 
ctgatggaa ccaacaaaa tcgaactgg aagagagta 
ccaaaaaca ttgttaaga ctgcagctg tggctgttg 
gctccctca aagacacgg aatttgata ttcgtttca 
atccagatg tttactcac cgggtatca aacattgtg 
ataatctag acccgacca tttcgaagc aaagacagt 
tggttaaag acgctgctg aatttttgc ccaccgtgc 
aaattccca cttttattc gcgattgtt tagatcatt 
cttcggccc caatggacg gttttactt tatccgagg 
cgatgcata atcgcggaa ttaacatta gatatctcg 
gtaaagtca caaatttgt tagcaaaag taaaacagt 
tggaatatt tgcatagca ttgctgttg cggaattgc 
tgctaaggg ccgccaaac atatcttta ccgtttatt 
tgcaggggt ctgaaatga tgaatttgg ctgttgcga 
ttgctcact tcctcaact gtttcctat attctggcc 
aagttccca atccattgc aaggagttg atgagagca 
aaaacaaca ggaagcgca acaaacgtc gaggtaaat 
tgaatccgt tggcttgta acgacaata acgaatggg 
cgaatctca ccccaaaat cgctttggt cgcaattaa 
aacaagaat tgaaatctt atttcgatt atgatttgt 
ctaccaacg atattgatg caaccaccg aaacattct 
gtttacaaa aaatctcat tattgcgag gtttttgtc 
taaagaccg gaatccaga ttttattga gggattata 
atttcgatt caaagaata aacttattt tctacgaag 
aagatattc tgaatattt tcccaatcg tcaaacaca 
ttaatccga gagcaactg atgcttata atttctata 
caacgggac agaataaga tacaacaag ggtatctta 
aagatgggt acgagttaa ttagtgaag ccttgaatt 
tgttgaata atgtgtatg gggtgatgc atccggaaa 
ttgcacaat gtttgagaa tgattgctc gacttaatt 
atatcatgg gagagcaca ctgaagcta tggcctatc 
agcagaagg ctgttttga tgtcggaaa gggtcaacg 
gaatcgacc atccctaca caattaccg aatatgcac 
atctcgcct tatattgct tcgctaata gtcaagtta 
gtaccgccc tgaaattgt tatacaggg cgagatatc 
ttgccgtac tcgtgtgcg gtgagaatc atccagaag 
ttgccttgc ttgatagca atatcagtt taattcttc 
acgccgttg gcgaatacg aactttctt tgcgtttct 
tagaaaagg ccttagctt taaatatca aatattacg 
gggccaaat ctcttaaag tcgccgtca cttatcatc 
tagtggcga ggacccaaa gttgcatgg gtaacttcc 
ggtgtgcgt taaccaatg aaaaggaag cttacgcca 
tctataaac aacagctcg gggaaaatc acgaaaaga 
ccaaagaat cgtcagagt gtctcaaac acctgacac 
agcaagccg ttgtgttgc agaaaaaaa tgaacgaga 
tctatacgg ggaaaaatg gagcttcac tgccaccta 
tacaaatcc agccgcctt ccatgagtt cggtcttgg 
acatgttga atgtcatta atgggattt tgttcgttc 
agataagtc aagaggata tcgagaatt ttaaagccg 
aaatcgaga aaagacaac tgaacgaag agaataatg 
aagcggatg aggaagaga aagcaaaac cagaagctt 
tggaaaatt agtaggatt tttagatgt attgaaaag 
gatagtcgt ctcgttagt taatttcgt agtggttct 
tgtgcgtgc attttccaa cgagtctgt aagtaacat 
gcattttta tcgctcaat gtaatacta gtcaaaagt 
aagaaactg ccttccatt gtaataaaa tgttgttga 
cgattttaa gtgatctag tgttgtgga tttctttcc 
cgagtactg ctattgttg ttgttgttg taagttaaa 
tatgaaata tgctgttgt tttgtgata cgcattaat 
taatcgtag aaagttgtg attaaatag ccaaggatg 
gtataaatt tgcatgttg cctggacaa gggaaatta 
ttttttatt gtattttac tttgagtgc gccaacttt 
agtaaggtg cagttttct atgatagaa cagattttt 
ttgtaatct aaattttgt taaaataaa tccagttga 
ttaca
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