Status
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Gene information
General information
TC031022
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC003302,TC003301,TC003300,TC003298
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid13: 927709..986780
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031022
    iBeetle screen iB_00549
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 80.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection



    adult lethality - Number of animals showing the phenotype: 2 (41 dpi)
    Days post injection


    Pupal morphology
    wings shortened - Number of animals showing the phenotype: 4

    Adult morphology
    elytra not closed - Number of animals showing the phenotype: 3


    elytra shortened - Number of animals showing the phenotype: 3


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031022 RA
      MSILVAFVS PPRRGIPLL RPPSAKLGP RGRLQLVCV 
      SASLELTFE CSLVVAVIA VRALYAGTL APLWTTAPS 
      LPQRPLAAL CLLATLTIA ARCYASSNA THPLVQFSP 
      TPSSSLSPI DNSNARPHS STLVLSRLL SPPRPVQSD 
      TSVIVINSK KLLHNQNSN ETKTTRRKK PISARTKRE 
      LYASKYYNR IFEQTKRTS AEKSNEEKR ANVLVTSET 
      DNSVSDSDR VNYTTVNTA PENEHQNDS SFLDQEDAP 
      AAHSEGTER PMPPVHDHG NASLSDWGI LVLVLSAIV 
      LTVALGLIT CVLSHYRPV TAGEGAADG GTAAHPHDA 
      ARTPTTAPM LSVVSAGAA AGVGGMDRL RGGRAAREL 
      AATRDRQVR LHAVHTDIA INLPPNIAM PDGEEQHPF 
      TSSRLHIRD PEQESEIHQ KCIRPPPNR TVLESESPP 
      PYRSSSAGL LGSSSSSSS SGGGGEWSG GGGAPLVVR 
      CHSMSSSSS STATTKRPE AGSSGQKTD FLHRTVKIF 
      TGGKKGAGA TPTPPPVHT TLPVVIVPT RPRRTSTDH 
      QQQQQQQQQ GPRPKPSV
    • >TC031022 RB
      MSILVAFVS PPRRGIPLL RPPSAKLGP RGRLQLVCV 
      SASLELTFE CSLVVAVIA VRALYAGTL APLWTTAPS 
      LPQRPLAAL CLLATLTIA ARCYASSNA THPLVQFSP 
      TPSSSLSPI DNSNARPHS STLVLSRLL SPPRPVQSD 
      TSVIVINSK KLLHNQNSN ETKTTRRKK PISARTKRE 
      LYASKYYNR IFEQTKRTS AEKSNEEKR ANVLVTSET 
      DNSVSDSDR VNYTTVNTA PENEHQNDS SFLDQEDAP 
      AAHSEGTER PMPPVHDHG NASLSDWGI LVLVLSAIV 
      LTVALGLIT CVLSHYRPV TAGEGAADG GTAAHPHDA 
      ARTPTTAPT FAAAHLIAV TTERSSMTV LFEKNNGAK 
      FQSSFADSF IKRAVRKCL TVMRQKLTI NRKMLSVVS 
      AGAAAGVGG MDRLRGGRA ARELAATRD RQVRLHAVH 
      TDIAINLPP NIAMPDGEE QHPFTSSRL HIRDPEQES 
      EIHQKCIRP PPNRTVLES ESPPPYRSS SAGLLGSSS 
      SSSSSGGGG EWSGGGGAP LVVRCHSMS SSSSSTATT 
      KRPEAGSSG QKTDFLHRT VKIFTGGKK GAGATPTPP 
      PVHTTLPVV IVPTRPRRT STDHQQQQQ QQQQGPRPK 
      PSV
    CDS sequence
    • >TC031022 RA
      atgtccatc cttgtcgcg tttgtaagt cctcctcgc 
      cgtgggatc ccactcttg cgccctcca tccgctaag 
      ctgggccct aggggccgt ttgcagtta gtgtgtgta 
      agcgcctcc ttggagttg acattcgag tgctctctg 
      gtggtcgcc gtcatcgca gtgagagcc ctttatgcc 
      gggacgttg gccccgctt tggaccacg gccccgagt 
      ctgccccag cgccccctg gccgccctc tgcctcctt 
      gccacttta acgatagcg gcaaggtgc tatgcgtcc 
      tcgaacgct acgcatcct ttagtccaa ttcagtccg 
      acgccctct tcttccctt agtccaatt gataattcc 
      aatgcccga ccgcattct tccacttta gtattaagt 
      aggttattg agtcctcct cgacctgta caaagtgat 
      actagcgta attgtaata aattctaaa aagttgttg 
      cataatcaa aattctaac gagacgaag accacgagg 
      cgcaaaaaa ccgattagc gctaggact aagcgcgaa 
      ctctacgct tcaaagtac tacaacagg atttttgaa 
      cagaccaag aggaccagc gcggaaaaa agcaacgag 
      gagaaaaga gcgaatgtg ctggtgacc agtgagact 
      gacaatagt gtgagtgat agtgataga gtgaactat 
      acgactgtc aacactgcg ccagaaaac gaacatcag 
      aacgatagc agcttctta gaccaggag gacgcacca 
      gccgcacac tctgaaggc acggaacgg cccatgcct 
      ccggtgcat gaccacggc aacgcttca ttgagcgat 
      tggggcatc ttggtgctg gtgttgtcc gcaattgtg 
      ctgacggtg gcgctgggg ctcatcacg tgcgtgctg 
      agccattat cgcccagtt actgctggc gagggggct 
      gcagacggg ggcacggcg gcccatccg cacgacgcc 
      gcgcgaaca ccgaccacc gcacccatg ctgtcggtt 
      gtgagcgcc ggcgccgcg gcgggggtg ggcggcatg 
      gaccgtctg cgaggcgga agagcggcc cgagagttg 
      gcggcgaca cgcgaccgc caagtgcga ctgcacgca 
      gttcacacc gacattgcc atcaacctg ccgcccaac 
      atagccatg ccagacggc gaggagcag caccctttc 
      acctcctcc agactgcac atccgggac ccggagcag 
      gagtccgaa atccaccag aaatgcatc cgaccgccg 
      cctaaccgc accgtgctc gagtcggag tcgccgccc 
      ccgtaccgc tcgagctcg gcgggcctg ttgggttcg 
      tcgtcgtcg tcgtcgtcg agcggcggc ggcggcgag 
      tggtcgggc gggggcggc gcgcctctg gtcgtgcgc 
      tgccactcg atgtcctcg tcgtcgagc tccaccgcc 
      accaccaaa cggccagaa gctggaagc agcggacaa 
      aagactgat tttttacat agaactgtt aaaattttc 
      accgggggc aagaagggg gcgggcgcg acgccgacg 
      ccgcccccc gtgcacacg accctgccg gtggtgatc 
      gtgccgacg cgcccccga cggacctcc accgaccac 
      cagcagcag cagcagcag cagcagcag ggccccagg 
      cccaagccc agtgtctga
    • >TC031022 RB
      atgtccatc cttgtcgcg tttgtaagt cctcctcgc 
      cgtgggatc ccactcttg cgccctcca tccgctaag 
      ctgggccct aggggccgt ttgcagtta gtgtgtgta 
      agcgcctcc ttggagttg acattcgag tgctctctg 
      gtggtcgcc gtcatcgca gtgagagcc ctttatgcc 
      gggacgttg gccccgctt tggaccacg gccccgagt 
      ctgccccag cgccccctg gccgccctc tgcctcctt 
      gccacttta acgatagcg gcaaggtgc tatgcgtcc 
      tcgaacgct acgcatcct ttagtccaa ttcagtccg 
      acgccctct tcttccctt agtccaatt gataattcc 
      aatgcccga ccgcattct tccacttta gtattaagt 
      aggttattg agtcctcct cgacctgta caaagtgat 
      actagcgta attgtaata aattctaaa aagttgttg 
      cataatcaa aattctaac gagacgaag accacgagg 
      cgcaaaaaa ccgattagc gctaggact aagcgcgaa 
      ctctacgct tcaaagtac tacaacagg atttttgaa 
      cagaccaag aggaccagc gcggaaaaa agcaacgag 
      gagaaaaga gcgaatgtg ctggtgacc agtgagact 
      gacaatagt gtgagtgat agtgataga gtgaactat 
      acgactgtc aacactgcg ccagaaaac gaacatcag 
      aacgatagc agcttctta gaccaggag gacgcacca 
      gccgcacac tctgaaggc acggaacgg cccatgcct 
      ccggtgcat gaccacggc aacgcttca ttgagcgat 
      tggggcatc ttggtgctg gtgttgtcc gcaattgtg 
      ctgacggtg gcgctgggg ctcatcacg tgcgtgctg 
      agccattat cgcccagtt actgctggc gagggggct 
      gcagacggg ggcacggcg gcccatccg cacgacgcc 
      gcgcgaaca ccgaccacc gcacccaca ttcgccgcg 
      gcacacttg atcgcagtt acgaccgaa cgttcgtcg 
      atgacggtc ctgttcgaa aaaaacaac ggcgcgaag 
      tttcaatct tcgttcgct gatagtttc atcaagcgg 
      gctgtgcgg aagtgtctc actgtcatg cgacaaaaa 
      ctgacaatt aacagaaag atgctgtcg gttgtgagc 
      gccggcgcc gcggcgggg gtgggcggc atggaccgt 
      ctgcgaggc ggaagagcg gcccgagag ttggcggcg 
      acacgcgac cgccaagtg cgactgcac gcagttcac 
      accgacatt gccatcaac ctgccgccc aacatagcc 
      atgccagac ggcgaggag cagcaccct ttcacctcc 
      tccagactg cacatccgg gacccggag caggagtcc 
      gaaatccac cagaaatgc atccgaccg ccgcctaac 
      cgcaccgtg ctcgagtcg gagtcgccg cccccgtac 
      cgctcgagc tcggcgggc ctgttgggt tcgtcgtcg 
      tcgtcgtcg tcgagcggc ggcggcggc gagtggtcg 
      ggcgggggc ggcgcgcct ctggtcgtg cgctgccac 
      tcgatgtcc tcgtcgtcg agctccacc gccaccacc 
      aaacggcca gaagctgga agcagcgga caaaagact 
      gatttttta catagaact gttaaaatt ttcaccggg 
      ggcaagaag ggggcgggc gcgacgccg acgccgccc 
      cccgtgcac acgaccctg ccggtggtg atcgtgccg 
      acgcgcccc cgacggacc tccaccgac caccagcag 
      cagcagcag cagcagcag cagggcccc aggcccaag 
      cccagtgtc tga
    mRNA sequence
    • >TC031022 RA
      cggtgcttc agcaggacg cgagcgacg cgagtccaa 
      ctgttatca aaggtgcga gtgccggcc ctgtgcgag 
      aacgccgcc cccagcggg cccatggtg cagtgtcgt 
      cgcgagtgc caccgctaa gatggtgtc cgagtaaga 
      agcggagtc gagcgagag tttgaactg cccccaaga 
      tggtgttgg gcagcacct tggtgcgat gcaatgtcc 
      atccttgtc gcgtttgta agtcctcct cgccgtggg 
      atcccactc ttgcgccct ccatccgct aagctgggc 
      cctaggggc cgtttgcag ttagtgtgt gtaagcgcc 
      tccttggag ttgacattc gagtgctct ctggtggtc 
      gccgtcatc gcagtgaga gccctttat gccgggacg 
      ttggccccg ctttggacc acggccccg agtctgccc 
      cagcgcccc ctggccgcc ctctgcctc cttgccact 
      ttaacgata gcggcaagg tgctatgcg tcctcgaac 
      gctacgcat cctttagtc caattcagt ccgacgccc 
      tcttcttcc cttagtcca attgataat tccaatgcc 
      cgaccgcat tcttccact ttagtatta agtaggtta 
      ttgagtcct cctcgacct gtacaaagt gatactagc 
      gtaattgta ataaattct aaaaagttg ttgcataat 
      caaaattct aacgagacg aagaccacg aggcgcaaa 
      aaaccgatt agcgctagg actaagcgc gaactctac 
      gcttcaaag tactacaac aggattttt gaacagacc 
      aagaggacc agcgcggaa aaaagcaac gaggagaaa 
      agagcgaat gtgctggtg accagtgag actgacaat 
      agtgtgagt gatagtgat agagtgaac tatacgact 
      gtcaacact gcgccagaa aacgaacat cagaacgat 
      agcagcttc ttagaccag gaggacgca ccagccgca 
      cactctgaa ggcacggaa cggcccatg cctccggtg 
      catgaccac ggcaacgct tcattgagc gattggggc 
      atcttggtg ctggtgttg tccgcaatt gtgctgacg 
      gtggcgctg gggctcatc acgtgcgtg ctgagccat 
      tatcgccca gttactgct ggcgagggg gctgcagac 
      gggggcacg gcggcccat ccgcacgac gccgcgcga 
      acaccgacc accgcaccc atgctgtcg gttgtgagc 
      gccggcgcc gcggcgggg gtgggcggc atggaccgt 
      ctgcgaggc ggaagagcg gcccgagag ttggcggcg 
      acacgcgac cgccaagtg cgactgcac gcagttcac 
      accgacatt gccatcaac ctgccgccc aacatagcc 
      atgccagac ggcgaggag cagcaccct ttcacctcc 
      tccagactg cacatccgg gacccggag caggagtcc 
      gaaatccac cagaaatgc atccgaccg ccgcctaac 
      cgcaccgtg ctcgagtcg gagtcgccg cccccgtac 
      cgctcgagc tcggcgggc ctgttgggt tcgtcgtcg 
      tcgtcgtcg tcgagcggc ggcggcggc gagtggtcg 
      ggcgggggc ggcgcgcct ctggtcgtg cgctgccac 
      tcgatgtcc tcgtcgtcg agctccacc gccaccacc 
      aaacggcca gaagctgga agcagcgga caaaagact 
      gatttttta catagaact gttaaaatt ttcaccggg 
      ggcaagaag ggggcgggc gcgacgccg acgccgccc 
      cccgtgcac acgaccctg ccggtggtg atcgtgccg 
      acgcgcccc cgacggacc tccaccgac caccagcag 
      cagcagcag cagcagcag cagggcccc aggcccaag 
      cccagtgtc tgaccacac gttacggcc cctaggtga 
      ctttttcaa tttctagtt taaagaaga agcgaaacg 
      aacgaccga tccggactc aagccactc attcaacga 
      acggacgtt tgctgcttg cgcttacaa gacgagtga 
      tacagacgc ttttaaatg tggttcttc acatacatg 
      agtgtttac cgaactctc tctctctct gatgatcat 
      gttggaata tcggccaag tatttgtta tatgtaccg 
      tgtacatcc aaatgataa ttactcaga attatggat 
      aactgtagg ttaggacac aaaacgact caacagttc 
      ttctcagta gttaatagt caatgcgac cagttttca 
      gaacaacga atttaattc tcaattgac aaaaaaaaa 
      aaacaaagt tgtagagaa ttaaatttg ctacagaca 
      tcgtatttt catctttaa cggtttagg tataaatta 
      attttcaat acttgacca gcggtaaaa tgaaacgaa 
      accgtcgct tgagcgtct aaagaaaat ttaattctc 
      tacaacttt ctcccaaca cttttcttg tatctttac 
      tggttttcg ttttgaaaa ataagggtc aaagtgcaa 
      attttaaat gtacaggct gtcccatgg taaaattca 
      ttattcact tgtaaatgt taaaaaatt aatgatttt 
      ttttataga ttgcagtac ttatccctg cctcaccta 
      aaattatct tcaaatata caggtgttt cgaaaatgt 
      ttcggattg gttgatttt gcttgttac taaaatata 
      tattttttt ttaacaaaa acaagctca aaaactaag 
      attcccaga aaagaatgt tcaaacttg agtcataac 
      acttaccaa tttgttggg acttcctgt ataattttt 
      tctcacgtt tctcgtaaa aatgttacc tttgattat 
      ttgaatttc cacgagtca ctttgggtg cgcttttgt 
      gtcctaact tatagtcgt ccatagcag aatcaatca 
      aaagaatat ttggcggcg catgcgcag cgcatagat 
      atttcaaca acgcacgac ttgacacta gaacattgt 
      tctgttttt aaatattgt ttattgaat cgattggga 
      tattgaagt gcaagaaga agaagaaga aacatgcta 
      aaagtgttc atcttatag tcagtcagt cagtcagtc 
      agccgtcgt caaaatagc tccaactga aatataatc 
      atcacacat cacattccg aagccggac ctgcactcg 
      ccagctgat ctcagacac tgttgaggg gcttccaaa 
      gccccgcag gtccgggct cggactcta tagcaacaa 
      aatgttaaa ttatataat tattatatt acaaacaaa 
      tattgtata tggtagaca acaccgtgt aatatagtg 
      gaaacataa gcatatgtg atgataggg tgcttggga 
      acgcatatg tgcatataa ctttgccga aacttattt 
      attttaatt gcaatcttt tcaggtgca atcaatcaa 
      tctcattag tattttatt cgcgcgcgc tctgtacat 
      atttattat tgtgcctcg ctttcacga ttatttcat 
      tccatctct aattaggaa aattccgac cgctttgta 
      tagcctcct caatgcacc aaaaccaat caatctact 
      tatatttat tttatcgtc gttccacga ggaaagttt 
      gttgcatgt atgatgccc aagttatta aaaagtctt 
      cataacaac aagaaaatg tgattataa ttaaatttt 
      attattaaa aagtgaata ataacaaat tgaaactgt 
      ttttgtatt agtgaatta ttaaaaaag aagtcgact 
      ttcaagtgt atacttatc tgtttattt ttctgttct 
      ctttctctt ctaagaaga ttttttgtt tagagggac 
      aattgtgta atgtgatta agtaaacat gattcatga 
      aataataaa tcactttca ttaaa
    • >TC031022 RB
      cggtgcttc agcaggacg cgagcgacg cgagtccaa 
      ctgttatca aaggtgcga gtgccggcc ctgtgcgag 
      aacgccgcc cccagcggg cccatggtg cagtgtcgt 
      cgcgagtgc caccgctaa gatggtgtc cgagtaaga 
      agcggagtc gagcgagag tttgaactg cccccaaga 
      tggtgttgg gcagcacct tggtgcgat gcaatgtcc 
      atccttgtc gcgtttgta agtcctcct cgccgtggg 
      atcccactc ttgcgccct ccatccgct aagctgggc 
      cctaggggc cgtttgcag ttagtgtgt gtaagcgcc 
      tccttggag ttgacattc gagtgctct ctggtggtc 
      gccgtcatc gcagtgaga gccctttat gccgggacg 
      ttggccccg ctttggacc acggccccg agtctgccc 
      cagcgcccc ctggccgcc ctctgcctc cttgccact 
      ttaacgata gcggcaagg tgctatgcg tcctcgaac 
      gctacgcat cctttagtc caattcagt ccgacgccc 
      tcttcttcc cttagtcca attgataat tccaatgcc 
      cgaccgcat tcttccact ttagtatta agtaggtta 
      ttgagtcct cctcgacct gtacaaagt gatactagc 
      gtaattgta ataaattct aaaaagttg ttgcataat 
      caaaattct aacgagacg aagaccacg aggcgcaaa 
      aaaccgatt agcgctagg actaagcgc gaactctac 
      gcttcaaag tactacaac aggattttt gaacagacc 
      aagaggacc agcgcggaa aaaagcaac gaggagaaa 
      agagcgaat gtgctggtg accagtgag actgacaat 
      agtgtgagt gatagtgat agagtgaac tatacgact 
      gtcaacact gcgccagaa aacgaacat cagaacgat 
      agcagcttc ttagaccag gaggacgca ccagccgca 
      cactctgaa ggcacggaa cggcccatg cctccggtg 
      catgaccac ggcaacgct tcattgagc gattggggc 
      atcttggtg ctggtgttg tccgcaatt gtgctgacg 
      gtggcgctg gggctcatc acgtgcgtg ctgagccat 
      tatcgccca gttactgct ggcgagggg gctgcagac 
      gggggcacg gcggcccat ccgcacgac gccgcgcga 
      acaccgacc accgcaccc acattcgcc gcggcacac 
      ttgatcgca gttacgacc gaacgttcg tcgatgacg 
      gtcctgttc gaaaaaaac aacggcgcg aagtttcaa 
      tcttcgttc gctgatagt ttcatcaag cgggctgtg 
      cggaagtgt ctcactgtc atgcgacaa aaactgaca 
      attaacaga aagatgctg tcggttgtg agcgccggc 
      gccgcggcg ggggtgggc ggcatggac cgtctgcga 
      ggcggaaga gcggcccga gagttggcg gcgacacgc 
      gaccgccaa gtgcgactg cacgcagtt cacaccgac 
      attgccatc aacctgccg cccaacata gccatgcca 
      gacggcgag gagcagcac cctttcacc tcctccaga 
      ctgcacatc cgggacccg gagcaggag tccgaaatc 
      caccagaaa tgcatccga ccgccgcct aaccgcacc 
      gtgctcgag tcggagtcg ccgcccccg taccgctcg 
      agctcggcg ggcctgttg ggttcgtcg tcgtcgtcg 
      tcgtcgagc ggcggcggc ggcgagtgg tcgggcggg 
      ggcggcgcg cctctggtc gtgcgctgc cactcgatg 
      tcctcgtcg tcgagctcc accgccacc accaaacgg 
      ccagaagct ggaagcagc ggacaaaag actgatttt 
      ttacataga actgttaaa attttcacc gggggcaag 
      aagggggcg ggcgcgacg ccgacgccg ccccccgtg 
      cacacgacc ctgccggtg gtgatcgtg ccgacgcgc 
      ccccgacgg acctccacc gaccaccag cagcagcag 
      cagcagcag cagcagggc cccaggccc aagcccagt 
      gtctgacca cacgttacg gcccctagg tgacttttt 
      caatttcta gtttaaaga agaagcgaa acgaacgac 
      cgatccgga ctcaagcca ctcattcaa cgaacggac 
      gtttgctgc ttgcgctta caagacgag tgatacaga 
      cgcttttaa atgtggttc ttcacatac atgagtgtt 
      taccgaact ctctctctc tctgatgat catgttgga 
      atatcggcc aagtatttg ttatatgta ccgtgtaca 
      tccaaatga taattactc agaattatg gataactgt 
      aggttagga cacaaaacg actcaacag ttcttctca 
      gtagttaat agtcaatgc gaccagttt tcagaacaa 
      cgaatttaa ttctcaatt gacaaaaaa aaaaaacaa 
      agttgtaga gaattaaat ttgctacag acatcgtat 
      tttcatctt taacggttt aggtataaa ttaattttc 
      aatacttga ccagcggta aaatgaaac gaaaccgtc 
      gcttgagcg tctaaagaa aatttaatt ctctacaac 
      tttctccca acacttttc ttgtatctt tactggttt 
      tcgttttga aaaataagg gtcaaagtg caaatttta 
      aatgtacag gctgtccca tggtaaaat tcattattc 
      acttgtaaa tgttaaaaa attaatgat tttttttat 
      agattgcag tacttatcc ctgcctcac ctaaaatta 
      tcttcaaat atacaggtg tttcgaaaa tgtttcgga 
      ttggttgat tttgcttgt tactaaaat atatatttt 
      tttttaaca aaaacaagc tcaaaaact aagattccc 
      agaaaagaa tgttcaaac ttgagtcat aacacttac 
      caatttgtt gggacttcc tgtataatt ttttctcac 
      gtttctcgt aaaaatgtt acctttgat tatttgaat 
      ttccacgag tcactttgg gtgcgcttt tgtgtccta 
      acttatagt cgtccatag cagaatcaa tcaaaagaa 
      tatttggcg gcgcatgcg cagcgcata gatatttca 
      acaacgcac gacttgaca ctagaacat tgttctgtt 
      tttaaatat tgtttattg aatcgattg ggatattga 
      agtgcaaga agaagaaga agaaacatg ctaaaagtg 
      ttcatctta tagtcagtc agtcagtca gtcagccgt 
      cgtcaaaat agctccaac tgaaatata atcatcaca 
      catcacatt ccgaagccg gacctgcac tcgccagct 
      gatctcaga cactgttga ggggcttcc aaagccccg 
      caggtccgg gctcggact ctatagcaa caaaatgtt 
      aaattatat aattattat attacaaac aaatattgt 
      atatggtag acaacaccg tgtaatata gtggaaaca 
      taagcatat gtgatgata gggtgcttg ggaacgcat 
      atgtgcata taactttgc cgaaactta tttatttta 
      attgcaatc ttttcaggt gcaatcaat caatctcat 
      tagtatttt attcgcgcg cgctctgta catatttat 
      tattgtgcc tcgctttca cgattattt cattccatc 
      tctaattag gaaaattcc gaccgcttt gtatagcct 
      cctcaatgc accaaaacc aatcaatct acttatatt 
      tattttatc gtcgttcca cgaggaaag tttgttgca 
      tgtatgatg cccaagtta ttaaaaagt cttcataac 
      aacaagaaa atgtgatta taattaaat tttattatt 
      aaaaagtga ataataaca aattgaaac tgtttttgt 
      attagtgaa ttattaaaa aagaagtcg actttcaag 
      tgtatactt atctgttta tttttctgt tctctttct 
      cttctaaga agatttttt gtttagagg gacaattgt 
      gtaatgtga ttaagtaaa catgattca tgaaataat 
      aaatcactt tcattaaa