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Gene information
General information
TC003280 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 711370..713452
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC003280
    iBeetle screen iB_00546

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003280 RA
      MALTVQKIF TPSTLTRAF SVTAARKSG FNFELNETQ 
REMQAMARK FAREEIAPQ AAHFDRTGE YPIEILKKA 
WAVGLMNSH IPEHCGGLN QGVFDACLM GEELSWGCS 
GIGTVIGSS HLGQVPVIL NGTKEQQKK YLGRLIEEP 
LVAAYAVTE PGCGSDVNG VKTRAEKKG DEYVINGQK 
MWITNGGVA NWYFVLART NPDPKAPAS KAFTGFIVD 
RDTPGVTPG RKEMNMGQR ASDTRGVTF EEVRVPKEN 
VLLGEGAGF KIAMGAFDT TRPPVACGA TGVAQRCLD 
EATKYSLER KTFGVPIAY HQAVAFMLA DMAIGVETA 
RLAWMRAAW EADHGIRNS YSASIAKAY AGDVANKCA 
SDAVQIFGG AGFNSEYPV EKLMRDAKI YQIYEGTTQ 
IQKLVISRT VLEASKRMA
    CDS sequence
    • >TC003280 RA
      atggcctta accgtgcaa aagattttc acaccaagc 
acgctcacg cgtgcattt tcggtaaca gccgcccga 
aaatctggc ttcaatttt gaactgaat gaaactcaa 
cgagaaatg caagccatg gcacgcaaa ttcgctcgg 
gaggagatt gcacctcaa gcggctcat ttcgacaga 
accggggaa tacccgatt gaaattttg aaaaaagca 
tgggctgtg ggtttgatg aattcgcac attcccgaa 
cactgcggt ggcttgaat cagggcgtt tttgatgcc 
tgtttgatg ggggaagaa ctgtcttgg gggtgttca 
gggattggg actgtgatt ggttcgtca catctaggg 
caagtccct gtgattcta aacgggaca aaagaacaa 
caaaagaag tatttgggg cggttgatt gaggaacct 
ttagttgct gcttatgcc gtcacagag ccgggctgt 
ggctccgac gtgaacggt gtcaaaacc cgagctgag 
aaaaaaggt gatgagtac gtaatcaac ggccaaaaa 
atgtggata acaaacgga ggcgtcgct aactggtac 
tttgtcctt gctcggact aaccctgac cccaaagcc 
cccgctagt aaagccttc actgggttt atcgtcgat 
agagacacc cctggggta acccccggc cgtaaagaa 
atgaacatg gggcaacga gcctccgac acccgcgga 
gtaaccttt gaagaagtg cgcgttccg aaggaaaac 
gtccttttg ggcgaaggc gccgggttt aaaatcgcc 
atgggggcg tttgacacc acgcgcccc ccggtggct 
tgtggggcc actggtgta gcacagagg tgtcttgac 
gaagctacg aaatattcg ctcgaacgt aaaacgttc 
ggtgttccc attgcctac caccaagct gtggctttt 
atgttggct gatatggcc attggggtt gagacggcc 
aggttggcg tggatgagg gccgcctgg gaggctgat 
catgggatt aggaacagt tattcggct tcgattgcg 
aaggcttat gctggagat gtggccaat aagtgtgct 
tcagacgcg gtgcagatt tttggaggt gctgggttt 
aatagtgag tatccggtg gagaagctg atgagggat 
gcgaagatt taccagatt tatgagggg acgacgcag 
attcagaag ttggtgatt tcgaggact gtgctcgag 
gcgagcaaa agaatggct tag
    mRNA sequence
    • >TC003280 RA
      ccatcaagg tcacaaatt ttaccgatt catcatttt 
tgtttacgt tgtaataat caaaagatt acattgaga 
gcttctaat tggtgcaaa aatctattt ttattgacc 
catcgttac agaaccgtt taattgcat taagagtcg 
ttctcgctt cgcttgaca aaacacacc caaaatggc 
cttaaccgt gcaaaagat tttcacacc aagcacgct 
cacgcgtgc attttcggt aacagccgc ccgaaaatc 
tggcttcaa ttttgaact gaatgaaac tcaacgaga 
aatgcaagc catggcacg caaattcgc tcgggagga 
gattgcacc tcaagcggc tcatttcga cagaaccgg 
ggaataccc gattgaaat tttgaaaaa agcatgggc 
tgtgggttt gatgaattc gcacattcc cgaacactg 
cggtggctt gaatcaggg cgtttttga tgcctgttt 
gatggggga agaactgtc ttgggggtg ttcagggat 
tgggactgt gattggttc gtcacatct agggcaagt 
ccctgtgat tctaaacgg gacaaaaga acaacaaaa 
gaagtattt ggggcggtt gattgagga acctttagt 
tgctgctta tgccgtcac agagccggg ctgtggctc 
cgacgtgaa cggtgtcaa aacccgagc tgagaaaaa 
aggtgatga gtacgtaat caacggcca aaaaatgtg 
gataacaaa cggaggcgt cgctaactg gtactttgt 
ccttgctcg gactaaccc tgaccccaa agcccccgc 
tagtaaagc cttcactgg gtttatcgt cgatagaga 
cacccctgg ggtaacccc cggccgtaa agaaatgaa 
catggggca acgagcctc cgacacccg cggagtaac 
ctttgaaga agtgcgcgt tccgaagga aaacgtcct 
tttgggcga aggcgccgg gtttaaaat cgccatggg 
ggcgtttga caccacgcg ccccccggt ggcttgtgg 
ggccactgg tgtagcaca gaggtgtct tgacgaagc 
tacgaaata ttcgctcga acgtaaaac gttcggtgt 
tcccattgc ctaccacca agctgtggc ttttatgtt 
ggctgatat ggccattgg ggttgagac ggccaggtt 
ggcgtggat gagggccgc ctgggaggc tgatcatgg 
gattaggaa cagttattc ggcttcgat tgcgaaggc 
ttatgctgg agatgtggc caataagtg tgcttcaga 
cgcggtgca gatttttgg aggtgctgg gtttaatag 
tgagtatcc ggtggagaa gctgatgag ggatgcgaa 
gatttacca gatttatga ggggacgac gcagattca 
gaagttggt gatttcgag gactgtgct cgaggcgag 
caaaagaat ggcttaggt tttagaatg aaaaaatca 
aatttttat acatattgt tacaacctg agtgtaata 
aagttttat taaaaac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY