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Gene information
General information
TC003280 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid13: 711370..713452
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003280
    iBeetle screen iB_00546
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003280 RA
      MALTVQKIF TPSTLTRAF SVTAARKSG FNFELNETQ 
      REMQAMARK FAREEIAPQ AAHFDRTGE YPIEILKKA 
      WAVGLMNSH IPEHCGGLN QGVFDACLM GEELSWGCS 
      GIGTVIGSS HLGQVPVIL NGTKEQQKK YLGRLIEEP 
      LVAAYAVTE PGCGSDVNG VKTRAEKKG DEYVINGQK 
      MWITNGGVA NWYFVLART NPDPKAPAS KAFTGFIVD 
      RDTPGVTPG RKEMNMGQR ASDTRGVTF EEVRVPKEN 
      VLLGEGAGF KIAMGAFDT TRPPVACGA TGVAQRCLD 
      EATKYSLER KTFGVPIAY HQAVAFMLA DMAIGVETA 
      RLAWMRAAW EADHGIRNS YSASIAKAY AGDVANKCA 
      SDAVQIFGG AGFNSEYPV EKLMRDAKI YQIYEGTTQ 
      IQKLVISRT VLEASKRMA
    CDS sequence
    • >TC003280 RA
      atggcctta accgtgcaa aagattttc acaccaagc 
      acgctcacg cgtgcattt tcggtaaca gccgcccga 
      aaatctggc ttcaatttt gaactgaat gaaactcaa 
      cgagaaatg caagccatg gcacgcaaa ttcgctcgg 
      gaggagatt gcacctcaa gcggctcat ttcgacaga 
      accggggaa tacccgatt gaaattttg aaaaaagca 
      tgggctgtg ggtttgatg aattcgcac attcccgaa 
      cactgcggt ggcttgaat cagggcgtt tttgatgcc 
      tgtttgatg ggggaagaa ctgtcttgg gggtgttca 
      gggattggg actgtgatt ggttcgtca catctaggg 
      caagtccct gtgattcta aacgggaca aaagaacaa 
      caaaagaag tatttgggg cggttgatt gaggaacct 
      ttagttgct gcttatgcc gtcacagag ccgggctgt 
      ggctccgac gtgaacggt gtcaaaacc cgagctgag 
      aaaaaaggt gatgagtac gtaatcaac ggccaaaaa 
      atgtggata acaaacgga ggcgtcgct aactggtac 
      tttgtcctt gctcggact aaccctgac cccaaagcc 
      cccgctagt aaagccttc actgggttt atcgtcgat 
      agagacacc cctggggta acccccggc cgtaaagaa 
      atgaacatg gggcaacga gcctccgac acccgcgga 
      gtaaccttt gaagaagtg cgcgttccg aaggaaaac 
      gtccttttg ggcgaaggc gccgggttt aaaatcgcc 
      atgggggcg tttgacacc acgcgcccc ccggtggct 
      tgtggggcc actggtgta gcacagagg tgtcttgac 
      gaagctacg aaatattcg ctcgaacgt aaaacgttc 
      ggtgttccc attgcctac caccaagct gtggctttt 
      atgttggct gatatggcc attggggtt gagacggcc 
      aggttggcg tggatgagg gccgcctgg gaggctgat 
      catgggatt aggaacagt tattcggct tcgattgcg 
      aaggcttat gctggagat gtggccaat aagtgtgct 
      tcagacgcg gtgcagatt tttggaggt gctgggttt 
      aatagtgag tatccggtg gagaagctg atgagggat 
      gcgaagatt taccagatt tatgagggg acgacgcag 
      attcagaag ttggtgatt tcgaggact gtgctcgag 
      gcgagcaaa agaatggct tag
    mRNA sequence
    • >TC003280 RA
      ccatcaagg tcacaaatt ttaccgatt catcatttt 
      tgtttacgt tgtaataat caaaagatt acattgaga 
      gcttctaat tggtgcaaa aatctattt ttattgacc 
      catcgttac agaaccgtt taattgcat taagagtcg 
      ttctcgctt cgcttgaca aaacacacc caaaatggc 
      cttaaccgt gcaaaagat tttcacacc aagcacgct 
      cacgcgtgc attttcggt aacagccgc ccgaaaatc 
      tggcttcaa ttttgaact gaatgaaac tcaacgaga 
      aatgcaagc catggcacg caaattcgc tcgggagga 
      gattgcacc tcaagcggc tcatttcga cagaaccgg 
      ggaataccc gattgaaat tttgaaaaa agcatgggc 
      tgtgggttt gatgaattc gcacattcc cgaacactg 
      cggtggctt gaatcaggg cgtttttga tgcctgttt 
      gatggggga agaactgtc ttgggggtg ttcagggat 
      tgggactgt gattggttc gtcacatct agggcaagt 
      ccctgtgat tctaaacgg gacaaaaga acaacaaaa 
      gaagtattt ggggcggtt gattgagga acctttagt 
      tgctgctta tgccgtcac agagccggg ctgtggctc 
      cgacgtgaa cggtgtcaa aacccgagc tgagaaaaa 
      aggtgatga gtacgtaat caacggcca aaaaatgtg 
      gataacaaa cggaggcgt cgctaactg gtactttgt 
      ccttgctcg gactaaccc tgaccccaa agcccccgc 
      tagtaaagc cttcactgg gtttatcgt cgatagaga 
      cacccctgg ggtaacccc cggccgtaa agaaatgaa 
      catggggca acgagcctc cgacacccg cggagtaac 
      ctttgaaga agtgcgcgt tccgaagga aaacgtcct 
      tttgggcga aggcgccgg gtttaaaat cgccatggg 
      ggcgtttga caccacgcg ccccccggt ggcttgtgg 
      ggccactgg tgtagcaca gaggtgtct tgacgaagc 
      tacgaaata ttcgctcga acgtaaaac gttcggtgt 
      tcccattgc ctaccacca agctgtggc ttttatgtt 
      ggctgatat ggccattgg ggttgagac ggccaggtt 
      ggcgtggat gagggccgc ctgggaggc tgatcatgg 
      gattaggaa cagttattc ggcttcgat tgcgaaggc 
      ttatgctgg agatgtggc caataagtg tgcttcaga 
      cgcggtgca gatttttgg aggtgctgg gtttaatag 
      tgagtatcc ggtggagaa gctgatgag ggatgcgaa 
      gatttacca gatttatga ggggacgac gcagattca 
      gaagttggt gatttcgag gactgtgct cgaggcgag 
      caaaagaat ggcttaggt tttagaatg aaaaaatca 
      aatttttat acatattgt tacaacctg agtgtaata 
      aagttttat taaaaac