Status
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Gene information
General information
TC031020
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC003264,TC003268
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 531763..564729
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031020
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00544

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031020 RA
      MADISLIQD HTMDDCDEG VFEQGDCST DSSGDDELL 
      PPLSRVQKY AQAGNIYDR KMVARIVQD LFRTSPPEI 
      LAEELPSIM TIVRSLGEK DETMVRADL LDQVPHIAS 
      QALKYSNRV EALKNVTGD YLIPLVVRN LGSSENAVD 
      KAAHTTLIH LLEQGLITK QQAEIQVCP AILALSHEE 
      QIAEINTGA ITLMSRMAP LLGRDVTER VFLKRFSEM 
      CESSMFYTR KMCAAHIGD FAVVVGKQP FHRTLLPCY 
      IALCEDSIW GVRKSCAEV IMFISTVSA PEVRRRVLA 
      PVFARLLQD DCRWVQMSA FQALGPFII TYANPPLTS 
      TAYNSQGEL IFVNKDGQE FLMNVAKET NDVRFKYLP 
      RQPSPLSSD LSQSCSEVG IGDWQGVED VLQEKDDEC 
      ASSELESER LPEENFDLD SGSLIAQSD EDIKEIVET 
      VKNAVLSYR NVEQEHGVI GDNLKTNLS NANVISNLK 
      NASCDKLGS ELDAEFANL SLSIDEPST SKMCDDVAD 
      DGAETTVEQ NQVILNSES EKEDDNLHL YNSYNYWYI 
      RPEMPLDLS LIEEIQIDR GKVDDTTAG DTFAEINLN 
      ENLAESNKN DAPTDTASE SKEVAKTEP VQDIVPQVL 
      ISHYFLMTD SSFSSNIDN EMSYHCAYS LPAIALTLE 
      NKNWPLLKR TIELLASDM HYKVRRTVA SSLHELAFI 
      LGQDIASEH LTPIFEGFI KDLDEVRIG ILRNLAYFL 
      KIIKPAKRL ACLPRLQEF LKTETEWNW RFRHELAKQ 
      LLSIVTLFK PVDSAKHIG VIGEVLLFD VSAVRQIAI 
      SLITELLRH ISSKPDLSS LYLVRLVEK FAHSKKWKR 
      RQTFALLCY KLLSAKALP AEKFATEIM PHLLDLSWD 
      PVANVRLVV AKTIAQHVI PNEYFADPS NQHFDSLGT 
      VIRRLQADK DRDVRQYAE ISDKFESVS PTLSLDYID
    CDS sequence
    • >TC031020 RA
      atggcggac atttcctta atccaagat cacaccatg 
      gatgattgc gatgaaggt gtattcgaa caaggagat 
      tgttcaacg gacagttct ggcgatgac gagctttta 
      ccaccgtta tcccgcgtc cagaaatac gctcaagct 
      gggaacatc tacgatcgt aaaatggtt gcccgaatt 
      gttcaagat ttgtttcgt acatcacct ccggaaatt 
      ttagccgaa gaacttccg agtattatg accatcgtt 
      cgatcgctg ggtgagaaa gatgagacg atggttaga 
      gccgatctt ttggatcag gtaccacac atagcttca 
      caggcgttg aagtattca aatcgcgtg gaagcgttg 
      aaaaatgtt accggggat tatctgata ccgttggtt 
      gtacgcaat ttaggaagt tcagaaaat gctgttgat 
      aaggcggca catacaaca ctcatacac ctgctggaa 
      caaggatta ataacaaaa caacaggcg gaaatacaa 
      gtttgtcct gcgattttg gcgttgtcg catgaagaa 
      caaatcgct gaaataaac acaggggcc ataacattg 
      atgtcaaga atggcacca ctgctcgga agagatgtc 
      acagagagg gtgtttttg aagaggttt tcggaaatg 
      tgtgaaagt agtatgttt tacacgagg aaaatgtgt 
      gcggcacat atcggcgat tttgctgtt gttgtggga 
      aaacaaccg ttccacaga actttgttg ccgtgttat 
      atagcattg tgtgaagac tcaatatgg ggcgttcga 
      aagtcatgt gctgaagta ataatgttt atatcaact 
      gtaagtgca cccgaagta cgtcgaagg gtactagca 
      ccggttttt gcaagatta ttgcaagat gattgtcgt 
      tgggtccaa atgtcagca ttccaagca ttggggcct 
      tttattata acttacgcc aatcctcct ttaaccagc 
      acggcctat aatagtcaa ggcgaatta atttttgtg 
      aataaagat ggtcaagag tttttaatg aatgttgcc 
      aaagagact aacgatgtc cgattcaaa tacttgcca 
      cgtcagccg tctcctctc agttcagat ttaagccaa 
      tcgtgcagc gaagtagga attggcgat tggcaaggt 
      gttgaagat gtattgcaa gagaaagac gacgaatgt 
      gcgagtagc gaattggaa agcgagcga ttacctgag 
      gagaatttc gatttggat tcgggtagt ttgatagcg 
      caaagcgac gaagatata aaagagatt gtggaaacg 
      gtgaaaaat gctgtactc agctataga aacgtggaa 
      caagaacac ggtgtgata ggcgataac ttaaaaact 
      aacttaagc aatgcaaat gtgataagt aatttaaaa 
      aatgcgagt tgtgataaa ttaggtagc gaattagat 
      gccgaattt gcaaatttg agtctgagt atagacgaa 
      ccaagtacg tcaaaaatg tgtgatgat gtcgctgat 
      gatggtgcg gaaacaacc gtcgaacaa aatcaggta 
      attttgaat agtgagagt gaaaaagaa gacgataac 
      ttacacttg tacaattca tacaattat tggtatata 
      agacctgaa atgccatta gatctcagt ttaatcgag 
      gagattcaa atcgatcga ggtaaagtg gacgataca 
      acggcagga gatacattt gctgagatt aatttaaat 
      gaaaatttg gccgaaagt aacaaaaat gatgcccca 
      actgataca gcttcggag tcgaaggag gttgcgaaa 
      accgagccg gtgcaagac attgttcct caagtgttg 
      atatctcat tacttttta atgacggat tcttcgttt 
      tcctcaaat atagacaac gagatgtcg taccattgc 
      gcctattca ctgccggca atcgcacta acgttagaa 
      aataaaaat tggccactt ttaaaacgt accatcgaa 
      ttattggct agtgatatg cattacaaa gtacgaaga 
      actgtagcg agtagttta cacgaatta gcgtttatt 
      ttgggtcaa gacatcgca tcggaacat ttaacacca 
      attttcgaa ggctttatc aaggatttg gacgaagtc 
      cgaatagga attttgcga aatttggcc tacttttta 
      aagattatt aaaccggcc aaaagattg gcgtgcttg 
      cctcggcta caggagttt ttaaaaacg gaaactgaa 
      tggaattgg cgattccgt cacgagttg gctaagcag 
      ctcctgtct attgttaca ctcttcaaa ccagtggat 
      tcggccaaa catattggg gttattggg gaagttttg 
      ctatttgat gtttccgcc gtgcgccaa attgcgatt 
      tctttgatc accgaattg ttgaggcac atttcatca 
      aagcccgat ctcagctca ctttaccta gtcagatta 
      gtggagaaa tttgctcat tcgaaaaaa tggaagagg 
      agacaaacg tttgccctc ctgtgttac aaactttta 
      tcggccaaa gccttgcct gcggaaaaa tttgcaacc 
      gaaattatg cctcatttg ttggacttg agttgggat 
      ccagtggct aatgtgaga ttagttgtg gcaaaaaca 
      atcgcacaa catgttatt cccaacgag tattttgcg 
      gatcccagt aatcaacat ttcgatagt ttaggaact 
      gtaataagg aggctccaa gccgataag gatcgtgat 
      gtgcgtcag tatgcggaa atcagtgat aaatttgaa 
      tcggtctca cctacactt agtttggat tacattgac 
      taa
    mRNA sequence
    • >TC031020 RA
      cgggaatta aatattttt acgttggta tgagaatta 
      aaataagta tttttcgta cacagccac actcgagcc 
      atgacgaag ttttagctt cgaaataag aattaaaag 
      tgaaaagca agataaata atcgtttta tcaaagtaa 
      atgagtcaa gtagcgtat ttaaatgtg attaattgt 
      tgcaagtgt tgacgtatg ttgttgtat taatgaaaa 
      ttttaaatc acgtgcctt tacattgct atgttttga 
      tgctagtac aaaatggcg gacatttcc ttaatccaa 
      gatcacacc atggatgat tgcgatgaa ggtgtattc 
      gaacaagga gattgttca acggacagt tctggcgat 
      gacgagctt ttaccaccg ttatcccgc gtccagaaa 
      tacgctcaa gctgggaac atctacgat cgtaaaatg 
      gttgcccga attgttcaa gatttgttt cgtacatca 
      cctccggaa attttagcc gaagaactt ccgagtatt 
      atgaccatc gttcgatcg ctgggtgag aaagatgag 
      acgatggtt agagccgat cttttggat caggtacca 
      cacatagct tcacaggcg ttgaagtat tcaaatcgc 
      gtggaagcg ttgaaaaat gttaccggg gattatctg 
      ataccgttg gttgtacgc aatttagga agttcagaa 
      aatgctgtt gataaggcg gcacataca acactcata 
      cacctgctg gaacaagga ttaataaca aaacaacag 
      gcggaaata caagtttgt cctgcgatt ttggcgttg 
      tcgcatgaa gaacaaatc gctgaaata aacacaggg 
      gccataaca ttgatgtca agaatggca ccactgctc 
      ggaagagat gtcacagag agggtgttt ttgaagagg 
      ttttcggaa atgtgtgaa agtagtatg ttttacacg 
      aggaaaatg tgtgcggca catatcggc gattttgct 
      gttgttgtg ggaaaacaa ccgttccac agaactttg 
      ttgccgtgt tatatagca ttgtgtgaa gactcaata 
      tggggcgtt cgaaagtca tgtgctgaa gtaataatg 
      tttatatca actgtaagt gcacccgaa gtacgtcga 
      agggtacta gcaccggtt tttgcaaga ttattgcaa 
      gatgattgt cgttgggtc caaatgtca gcattccaa 
      gcattgggg ccttttatt ataacttac gccaatcct 
      cctttaacc agcacggcc tataatagt caaggcgaa 
      ttaattttt gtgaataaa gatggtcaa gagttttta 
      atgaatgtt gccaaagag actaacgat gtccgattc 
      aaatacttg ccacgtcag ccgtctcct ctcagttca 
      gatttaagc caatcgtgc agcgaagta ggaattggc 
      gattggcaa ggtgttgaa gatgtattg caagagaaa 
      gacgacgaa tgtgcgagt agcgaattg gaaagcgag 
      cgattacct gaggagaat ttcgatttg gattcgggt 
      agtttgata gcgcaaagc gacgaagat ataaaagag 
      attgtggaa acggtgaaa aatgctgta ctcagctat 
      agaaacgtg gaacaagaa cacggtgtg ataggcgat 
      aacttaaaa actaactta agcaatgca aatgtgata 
      agtaattta aaaaatgcg agttgtgat aaattaggt 
      agcgaatta gatgccgaa tttgcaaat ttgagtctg 
      agtatagac gaaccaagt acgtcaaaa atgtgtgat 
      gatgtcgct gatgatggt gcggaaaca accgtcgaa 
      caaaatcag gtaattttg aatagtgag agtgaaaaa 
      gaagacgat aacttacac ttgtacaat tcatacaat 
      tattggtat ataagacct gaaatgcca ttagatctc 
      agtttaatc gaggagatt caaatcgat cgaggtaaa 
      gtggacgat acaacggca ggagataca tttgctgag 
      attaattta aatgaaaat ttggccgaa agtaacaaa 
      aatgatgcc ccaactgat acagcttcg gagtcgaag 
      gaggttgcg aaaaccgag ccggtgcaa gacattgtt 
      cctcaagtg ttgatatct cattacttt ttaatgacg 
      gattcttcg ttttcctca aatatagac aacgagatg 
      tcgtaccat tgcgcctat tcactgccg gcaatcgca 
      ctaacgtta gaaaataaa aattggcca cttttaaaa 
      cgtaccatc gaattattg gctagtgat atgcattac 
      aaagtacga agaactgta gcgagtagt ttacacgaa 
      ttagcgttt attttgggt caagacatc gcatcggaa 
      catttaaca ccaattttc gaaggcttt atcaaggat 
      ttggacgaa gtccgaata ggaattttg cgaaatttg 
      gcctacttt ttaaagatt attaaaccg gccaaaaga 
      ttggcgtgc ttgcctcgg ctacaggag tttttaaaa 
      acggaaact gaatggaat tggcgattc cgtcacgag 
      ttggctaag cagctcctg tctattgtt acactcttc 
      aaaccagtg gattcggcc aaacatatt ggggttatt 
      ggggaagtt ttgctattt gatgtttcc gccgtgcgc 
      caaattgcg atttctttg atcaccgaa ttgttgagg 
      cacatttca tcaaagccc gatctcagc tcactttac 
      ctagtcaga ttagtggag aaatttgct cattcgaaa 
      aaatggaag aggagacaa acgtttgcc ctcctgtgt 
      tacaaactt ttatcggcc aaagccttg cctgcggaa 
      aaatttgca accgaaatt atgcctcat ttgttggac 
      ttgagttgg gatccagtg gctaatgtg agattagtt 
      gtggcaaaa acaatcgca caacatgtt attcccaac 
      gagtatttt gcggatccc agtaatcaa catttcgat 
      agtttagga actgtaata aggaggctc caagccgat 
      aaggatcgt gatgtgcgt cagtatgcg gaaatcagt 
      gataaattt gaatcggtc tcacctaca cttagtttg 
      gattacatt gactaaata aattacagt aaagttgtt 
      ttgttttta ttcttgggt ttatgaact ttgttgtaa 
      ttttaatta aatcttgtg agctatata cattgtaat 
      atttatcta taagttgta atataagta aaaacaata 
      a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY