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Gene information
General information
TC032293
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC003263
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 527260..531173
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032293
    iBeetle screen iB_00543

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032293 RA
      MGVCWCKQK HEEHDTYVP QTHSNATIQ PVSPVAPQY 
      YIRVAKAPD PQLVDKLVL ETLGVIATL VDNEQDPPP 
      SMLLLHNIA DNEEGFIQV VKSMIKVVP LSDPLGPSI 
      ITLLLDDCP LPSKESVFK VVDMLNVSR EAAIAGRLN 
      PSKERNTCV VLGCIAETL AGPRNLRIL NEHMLDYLL 
      TNLEPETDP NVVLFSLIA LEKFAQSSQ NKSTIMKRL 
      QVLNPCPIN ALEKWRNES HYIKRQVGF CAQWVLDNL 
      FVMNNRQYS YLTVNMENI NAMLNTSDV SEYLKISPD 
      GLEARCDAY SFESVRCTA QADSGIWYY EVCIITPGV 
      MQIGWATKN SNFLNHEGY GIGDDKYSL AYDGCRKLI 
      WYNAKSEPQ NLPAWQSGD ILGCFLDLN TPQVIFSIN 
      GMRLPPCTH VFTMAQSGF FAAASFMSF QQCRFNFGA 
      EPFRYPPSG EFSTFNDQG VLKPEDKIV MPRHIFLDQ 
      LRQQNIRED SCTLCYDKK ASVRLIPCE HSGFCPSCA 
      SQLVECPMC RAPFELVVE ENP
    CDS sequence
    • >TC032293 RA
      atgggtgtc tgctggtgt aagcaaaaa cacgaagaa 
      catgacact tatgttcct caaacccat agcaatgct 
      acaatccaa cccgtttca ccagtagcc ccccagtat 
      tacattcga gtcgccaaa gccccggac ccacaactc 
      gtcgataaa ctcgtcctg gaaacgctg ggcgttatt 
      gcaactctt gtggacaat gagcaagac ccgcccccg 
      tcaatgctc ctcctacac aacatcgcc gataacgag 
      gaaggcttc atccaagtc gtcaaatca atgattaaa 
      gtcgtgccg ttatcagac cctctaggc ccgtcaatt 
      atcacactt ttgctggac gattgtccc ctgccttcg 
      aaagaatca gtattcaag gtcgtcgat atgttgaac 
      gtgtcccgg gaggcggca atagccggg agactgaat 
      ccctccaaa gagagaaac acttgtgta gttctaggg 
      tgtatagcg gagacgtta gccggtcca aggaattta 
      agaatatta aacgaacac atgttagac tatttatta 
      accaatttg gagcctgaa actgatcca aatgttgtc 
      ctattttcc ctaatagct ttagaaaag tttgcccag 
      tctagtcaa aacaaatca acaattatg aagcggttg 
      caagtcttg aatccttgt ccgataaat gcgctagaa 
      aagtggcgg aatgagagt cactacatc aaaagacaa 
      gtcgggttt tgcgcccag tgggtcttg gacaattta 
      ttcgtgatg aacaatcgt caatattcc tacttaacc 
      gtgaatatg gagaacata aacgccatg ttaaacacc 
      agcgacgtc agcgagtac cttaaaatc tcaccagat 
      ggcttagag gcccgttgc gacgcctac tctttcgaa 
      agtgttcgt tgtacggct caggccgat tcaggaata 
      tggtactat gaagtttgt attataaca ccaggagtt 
      atgcaaatt ggatgggca acaaaaaac agcaatttt 
      cttaaccac gagggttat ggcattggc gatgacaag 
      tactcgctg gcttatgac ggttgtcgc aaattaatt 
      tggtacaat gccaaatcc gaaccccaa aatttgccc 
      gcatggcaa tctggcgat attttgggc tgtttcctc 
      gacttgaat acgccacaa gtcattttt tctataaac 
      ggtatgagg ttaccacca tgtactcat gtgttcact 
      atggctcaa tcgggtttt tttgccgct gctagtttt 
      atgtcattc caacaatgt cggtttaat ttcggagcg 
      gaaccgttt cgttatccg ccttccggg gaattttcc 
      actttcaat gatcagggt gttttaaag cccgaggat 
      aaaattgta atgccaagg catattttt ttggatcag 
      ttgagacaa caaaatatt agagaggat agttgcacg 
      ctttgttac gataagaag gcttcagtg cggctgatt 
      ccctgcgaa cactcgggt ttttgtccg tcgtgtgca 
      agtcagttg gtagaatgt cccatgtgt agagcaccc 
      ttcgaattg gtggtagaa gaaaatccc tga
    mRNA sequence
    • >TC032293 RA
      atcgtgtga cagttgttc agactatcc taaccaaaa 
      acgtgtttt atctcaaac tccttttcc tcttcgaaa 
      aatcagtgc aaatcggcg tgcttacgt aatccatgc 
      agttaatta atgggctta tccaaaccc gcctaatgc 
      agaaattca tatcggtat cgaatcacg ccagtgcta 
      attttaata tgttgatgt caatgatta caccaacat 
      aaaaacttg cataaacat tgagacaca actagcatg 
      tagtaaacc accatcaag tctttgtaa atacacttt 
      ccaccaccg caacgtaat gggtgtctg ctggtgtaa 
      gcaaaaaca cgaagaaca tgacactta tgttcctca 
      aacccatag caatgctac aatccaacc cgtttcacc 
      agtagcccc ccagtatta cattcgagt cgccaaagc 
      cccggaccc acaactcgt cgataaact cgtcctgga 
      aacgctggg cgttattgc aactcttgt ggacaatga 
      gcaagaccc gcccccgtc aatgctcct cctacacaa 
      catcgccga taacgagga aggcttcat ccaagtcgt 
      caaatcaat gattaaagt cgtgccgtt atcagaccc 
      tctaggccc gtcaattat cacactttt gctggacga 
      ttgtcccct gccttcgaa agaatcagt attcaaggt 
      cgtcgatat gttgaacgt gtcccggga ggcggcaat 
      agccgggag actgaatcc ctccaaaga gagaaacac 
      ttgtgtagt tctagggtg tatagcgga gacgttagc 
      cggtccaag gaatttaag aatattaaa cgaacacat 
      gttagacta tttattaac caatttgga gcctgaaac 
      tgatccaaa tgttgtcct attttccct aatagcttt 
      agaaaagtt tgcccagtc tagtcaaaa caaatcaac 
      aattatgaa gcggttgca agtcttgaa tccttgtcc 
      gataaatgc gctagaaaa gtggcggaa tgagagtca 
      ctacatcaa aagacaagt cgggttttg cgcccagtg 
      ggtcttgga caatttatt cgtgatgaa caatcgtca 
      atattccta cttaaccgt gaatatgga gaacataaa 
      cgccatgtt aaacaccag cgacgtcag cgagtacct 
      taaaatctc accagatgg cttagaggc ccgttgcga 
      cgcctactc tttcgaaag tgttcgttg tacggctca 
      ggccgattc aggaatatg gtactatga agtttgtat 
      tataacacc aggagttat gcaaattgg atgggcaac 
      aaaaaacag caattttct taaccacga gggttatgg 
      cattggcga tgacaagta ctcgctggc ttatgacgg 
      ttgtcgcaa attaatttg gtacaatgc caaatccga 
      accccaaaa tttgcccgc atggcaatc tggcgatat 
      tttgggctg tttcctcga cttgaatac gccacaagt 
      cattttttc tataaacgg tatgaggtt accaccatg 
      tactcatgt gttcactat ggctcaatc gggtttttt 
      tgccgctgc tagttttat gtcattcca acaatgtcg 
      gtttaattt cggagcgga accgtttcg ttatccgcc 
      ttccgggga attttccac tttcaatga tcagggtgt 
      tttaaagcc cgaggataa aattgtaat gccaaggca 
      tattttttt ggatcagtt gagacaaca aaatattag 
      agaggatag ttgcacgct ttgttacga taagaaggc 
      ttcagtgcg gctgattcc ctgcgaaca ctcgggttt 
      ttgtccgtc gtgtgcaag tcagttggt agaatgtcc 
      catgtgtag agcaccctt cgaattggt ggtagaaga 
      aaatccctg acgactaaa ccgcaacta ggagacata 
      atcaattgt tttaatagc gtatttttg tacaaattt 
      gttgaattt tttcataga cacgggttt attgtttta 
      ggttacaca tagaaggtt gtatttatt tagctaggc 
      tttactaca gattttagt cccagttgt tgatatttt 
      tataaaaaa aaattcacg aattttatt ttagcaatc 
      aggggtaat cgattttaa tttgacaat aacgataca 
      tagtgtcaa atttaaatg attttattt aaaaaacta 
      tataaccat gttatgaat aattatttt aaagtttaa 
      acataaaaa ataccacac tcttaaata aatctttga 
      tcaaagtgg ccttccaat cacagtcta ctttgatcg 
      gaaatttaa ttaaaagcg tggaaatac atgactata 
      ttttaatgt tgaaataaa agtattgct tcaag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY