Status
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Gene information
General information
TC003257 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 460761..464464
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003257
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00542

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    larva cuticle duplicated - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003257 RA
      MSLSASAEN LTVETQDYD GSSNMCLEL ALEGERLCK 
      AGDCRAGVA FFQAAIQAG TDDLRTLSA IYSQLGNAY 
      FYLGDYGKA MQYHKHDLT LARTMGDKL GEAKSSGNL 
      GNTLKVMGR FDEAVVCCK RHLELSREL GDRLSEGRA 
      LYNLGNVFH AKGKHIGRV GHKDPGDFS DDVKGCLQE 
      AVSYYEENL TLMRELGDI AAQGRACGN LGNTFYLLG 
      DFAQAIHYH EERLSIARQ FGDKAAERR AHSNLGNSH 
      IFMGQFEEA AHHYKRTLA LAQELGDHA VEAQACYSL 
      GNTYTLLRD YETAIEYHL RHLLIAQNL ADRIGEGRA 
      CWSLGNANA SLGNHEKAL QFAKKHLEI SKELGDPMG 
      EATAKMNIA DLRKILNLP DSPESCDSD SPKENDVTH 
      QRIQRVRRE SMEQLSLIK LTPDGKQRP GFTLPPMKS 
      APSPTKNED DDSFFDLLS RFQSKRMDD QRCSLTVDN 
      NKENTNVVN LPRNEGPDD LIDMIAGMQ SKRMDEQRV 
      ALPHLPGLQ SSSLQKLAE SRNNSVPDD NFLDQLVRC 
      QGSRLEDQR SPLPMPAVD VEADPPPAR KSGATVPDE 
      DFISLIMRF QSGRMDDQR AAVPRVEHR MANGNVPHA 
      TNGISNNFK SNSSGKKKK
    CDS sequence
    • >TC003257 RA
      atgtccctg agtgctagt gccgagaac ctgactgtg 
      gaaacacag gactatgac ggttcgagc aatatgtgc 
      ttagagttg gccttggag ggcgagcgt ctgtgcaag 
      gcgggcgac tgcagggca ggagtggct ttttttcag 
      gccgcaatc caggccgga actgacgac ttgcggacg 
      ctcagtgct atttacagt cagctcgga aatgcgtat 
      ttttatcta ggagattat ggcaaagcc atgcaatat 
      cacaaacat gatctcact ttggccagg acaatgggt 
      gataaactg ggcgaggcg aagtcttcg ggaaatctg 
      ggcaatact ctgaaggtg atggggcgg tttgatgaa 
      gctgtggtg tgttgtaaa cgacatttg gagctttcg 
      agagaattg ggcgatagg ttgagcgaa ggcagggcg 
      ttgtataat ttaggaaat gtttttcat gccaagggg 
      aaacatatc gggagggtg gggcacaag gatccgggg 
      gatttttca gatgacgtc aaagggtgt ctccaagaa 
      gctgtctct tattatgag gaaaactta acactgatg 
      cgtgaactg ggcgatata gcggcacaa ggaagagct 
      tgtggtaat ttgggcaat acgttttat ttattgggt 
      gatttcgcg caagcgata cattaccat gaagaaaga 
      ttatcaata gcgagacaa tttggtgat aaagcggcc 
      gaaagaaga gcacatagt aatctcgga aattcgcat 
      atttttatg ggacagttt gaagaagcc gcccatcat 
      tacaaacga actctagcc ctggcccag gaattaggc 
      gaccatgca gtcgaagct caggcttgt tatagttta 
      ggcaacact tatacgcta ttgagagat tacgaaact 
      gccattgaa tatcatttg agacatctt ttgatagcg 
      caaaatttg gccgatcgc attggggaa ggtagggcg 
      tgttggtca ttgggtaat gcaaacgca tcgttgggt 
      aatcacgaa aaggcgcta caatttgcc aaaaaacac 
      ctcgaaatt agtaaagag ttgggcgat ccaatgggt 
      gaagctaca gcgaaaatg aacattgct gacttacga 
      aaaatcctc aatttgcct gatagtccc gaatcgtgt 
      gacagtgat tccccgaaa gagaatgac gtcactcac 
      cagaggata caaagggtg agacgtgaa agcatggaa 
      cagcttagc ctgattaag ttgacaccc gacggcaaa 
      caacgtccc ggcttcaca cttccacca atgaaatca 
      gccccgtca ccaactaaa aacgaagat gacgattcc 
      ttcttcgat ttgttatcc cgcttccaa tcgaaacgt 
      atggacgac caacgttgt tccctcact gttgacaac 
      aacaaggaa aacaccaac gtggtgaat ctgcctcga 
      aatgaaggt cccgatgat ctgattgac atgattgcc 
      ggaatgcaa agcaaaaga atggatgag caaagagtt 
      gcactacca catcttcca ggattgcaa tcatcgtcg 
      ttacaaaag ttggccgaa tcgaggaat aatagtgtt 
      cccgatgat aatttcctg gatcagttg gtgcgttgt 
      caaggttca agacttgag gatcagaga tcgccgctc 
      ccgatgccg gcggttgat gtggaggcc gatccgcca 
      ccggcaagg aaaagtggg gcgactgtg ccagatgag 
      gattttatc tcgttgatt atgaggttt cagtcgggg 
      agaatggat gatcaaagg gcggctgtg ccccgagtg 
      gaacacaga atggccaat ggtaacgtc ccgcatgca 
      acaaatggt attagtaat aattttaaa agcaattct 
      tcgggcaag aagaagaag taa
    mRNA sequence
    • >TC003257 RA
      tgacagttg caggaagag gagtgttgt gtgttttga 
      caattgtgc gtttttatt gttgtttat ttgcgtaac 
      aatgaaaga atgagcggg tgattcata cctcaagtg 
      cttttaatt gctttaatt aatcgaatt aacgttaaa 
      aatgtccct gagtgctag tgccgagaa cctgactgt 
      ggaaacaca ggactatga cggttcgag caatatgtg 
      cttagagtt ggccttgga gggcgagcg tctgtgcaa 
      ggcgggcga ctgcagggc aggagtggc tttttttca 
      ggccgcaat ccaggccgg aactgacga cttgcggac 
      gctcagtgc tatttacag tcagctcgg aaatgcgta 
      tttttatct aggagatta tggcaaagc catgcaata 
      tcacaaaca tgatctcac tttggccag gacaatggg 
      tgataaact gggcgaggc gaagtcttc gggaaatct 
      gggcaatac tctgaaggt gatggggcg gtttgatga 
      agctgtggt gtgttgtaa acgacattt ggagctttc 
      gagagaatt gggcgatag gttgagcga aggcagggc 
      gttgtataa tttaggaaa tgtttttca tgccaaggg 
      gaaacatat cgggagggt ggggcacaa ggatccggg 
      ggatttttc agatgacgt caaagggtg tctccaaga 
      agctgtctc ttattatga ggaaaactt aacactgat 
      gcgtgaact gggcgatat agcggcaca aggaagagc 
      ttgtggtaa tttgggcaa tacgtttta tttattggg 
      tgatttcgc gcaagcgat acattacca tgaagaaag 
      attatcaat agcgagaca atttggtga taaagcggc 
      cgaaagaag agcacatag taatctcgg aaattcgca 
      tatttttat gggacagtt tgaagaagc cgcccatca 
      ttacaaacg aactctagc cctggccca ggaattagg 
      cgaccatgc agtcgaagc tcaggcttg ttatagttt 
      aggcaacac ttatacgct attgagaga ttacgaaac 
      tgccattga atatcattt gagacatct tttgatagc 
      gcaaaattt ggccgatcg cattgggga aggtagggc 
      gtgttggtc attgggtaa tgcaaacgc atcgttggg 
      taatcacga aaaggcgct acaatttgc caaaaaaca 
      cctcgaaat tagtaaaga gttgggcga tccaatggg 
      tgaagctac agcgaaaat gaacattgc tgacttacg 
      aaaaatcct caatttgcc tgatagtcc cgaatcgtg 
      tgacagtga ttccccgaa agagaatga cgtcactca 
      ccagaggat acaaagggt gagacgtga aagcatgga 
      acagcttag cctgattaa gttgacacc cgacggcaa 
      acaacgtcc cggcttcac acttccacc aatgaaatc 
      agccccgtc accaactaa aaacgaaga tgacgattc 
      cttcttcga tttgttatc ccgcttcca atcgaaacg 
      tatggacga ccaacgttg ttccctcac tgttgacaa 
      caacaagga aaacaccaa cgtggtgaa tctgcctcg 
      aaatgaagg tcccgatga tctgattga catgattgc 
      cggaatgca aagcaaaag aatggatga gcaaagagt 
      tgcactacc acatcttcc aggattgca atcatcgtc 
      gttacaaaa gttggccga atcgaggaa taatagtgt 
      tcccgatga taatttcct ggatcagtt ggtgcgttg 
      tcaaggttc aagacttga ggatcagag atcgccgct 
      cccgatgcc ggcggttga tgtggaggc cgatccgcc 
      accggcaag gaaaagtgg ggcgactgt gccagatga 
      ggattttat ctcgttgat tatgaggtt tcagtcggg 
      gagaatgga tgatcaaag ggcggctgt gccccgagt 
      ggaacacag aatggccaa tggtaacgt cccgcatgc 
      aacaaatgg tattagtaa taattttaa aagcaattc 
      ttcgggcaa gaagaagaa gtaatacga ataagttag 
      agtaagtaa atttgaaaa ccccatatc tgaattttt 
      tatttcgat tgactgcca ttggagcat gtacgataa 
      aaagagaaa atctaacgt aaagtatat gttatttta 
      taattttat atattttta tatatgttt agtaaataa 
      tattgatgt atctat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY