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Gene information
General information
TC032285
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC003229
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid13: 115989..117941
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC032285
      iBeetle screen iB_00538
      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

      adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 50
      egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      remarks: bad quality of preparation,
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality early (L5/6) - Number of animals showing the phenotype: 4 (11 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC032285 RA
        MKRKAVVFC ALLLSTFGH ELLNRQKPS HALLSRVHS 
        KEECRLECI PDSGNSSNL QIFINTTTN LVAVASTKT 
        EVALILDVN SGLVGLFNA ISHSVIIIN IERSTRVAF 
        SQNSTWNKS ETFQQYFQT LTSLKILYE QGEHQGEKF 
        SDSNITQEI AIQITFVLK ILTSDKYSI QVKEALEVL 
        VDVSKNLLK SLREVTEIV KSQVINTNL EIKELIGIV 
        NGLASFVKR LNGGISLLT KREIRLDLD IIINSKTAI 
        DVFLYILLQ LKSSKLRLN EVLRDGFDS PQGFFFLIA 
        KLGGNSTRG INGILTILS KIIALNQRT SIESVVIAL 
        IQFFGNSNG KNFGKEWQT TIENLSRTI KQLHLNDIL 
        KKVVKQLQD IRNEKWTEN HVNLSKIVK ILTNASTPI 
        DSIVKILAI LQKVVPINV SLPFLLIEF VRLLTTKTG 
        LELIVALIP QIKTNTKTS KSEILRSIL RAIDIKNSG 
        NIGKALKNV PIVETIYSE LEKSVNKEA HLELNVVIG 
        EKASRHVLL EIRRILIKA IRDESENDN SGHSSLENG 
        HQHSKEHGK VKQNGKSH
      CDS sequence
      • >TC032285 RA
        atgaagcga aaagcagtg gtgttttgt gccttgtta 
        ttgagcact tttggacac gagctttta aatcgtcaa 
        aaaccatcc catgctctt ttatcgcgt gtccattct 
        aaagaagaa tgccgtctc gaatgcata ccagattca 
        ggaaactca tcgaatttg caaattttt atcaacaca 
        actacaaat ttagtggct gtggctagt actaaaacc 
        gaagttgcg ttaattctg gatgtaaat tcgggattg 
        gtcggtctg tttaatgca atttctcat tcggtgata 
        ataattaat attgaacga agcaccaga gttgcattt 
        tctcaaaat tctacatgg aacaagtct gaaactttc 
        caacaatac tttcaaact ctaacttcg ttaaaaatt 
        ttatacgaa caaggggaa catcaaggc gaaaagttt 
        tccgatagt aatattaca caagaaatt gcgatacaa 
        ataactttc gttttaaaa attctaacg tctgataaa 
        tattcgatt caagttaaa gaagcatta gaggtgctt 
        gttgatgtt tcaaaaaat ttgctaaaa tctttgaga 
        gaagtcacc gaaattgta aaaagtcaa gttattaat 
        acaaacctt gaaataaaa gaattaatt ggaattgtg 
        aacggttta gctagtttt gtaaaacgc ctaaacgga 
        ggcatttct cttttaaca aaacgagaa ataagactc 
        gacttggac attattata aattctaaa accgcaata 
        gatgttttc ctttacatt ttactacaa ctgaaatcg 
        agtaaatta cgtttgaat gaagttcta cgtgatggg 
        tttgactca cctcaaggc ttttttttc ctaattgca 
        aaattagga ggaaattct acacgcggc atcaatggg 
        attttgaca atactttca aaaataatc gctttgaat 
        caacgaact tccattgag tctgttgtt atcgctctg 
        atacaattt tttggtaat tcaaatggc aaaaatttt 
        ggtaaagaa tggcaaact acgatcgaa aatttatca 
        cgtactatt aaacaactt cacttgaat gatattcta 
        aaaaaagtc gtgaaacaa ctccaggat attcgcaac 
        gaaaaatgg accgaaaat cacgttaac ttatccaaa 
        atcgttaaa attttaact aatgcttca accccaatt 
        gattcaatt gttaaaatt cttgcaatt cttcaaaaa 
        gttgttccg attaatgtt tcgctccct ttcttgtta 
        atcgaattt gtgcgtctt ttaacgacg aaaactgga 
        cttgaactt attgttgct ttaataccg caaattaaa 
        accaacacg aaaacttca aagagtgaa attcttcga 
        agcattttg cgtgctatt gatatcaaa aattcgggt 
        aatatcggt aaagcatta aaaaatgta ccaatagtt 
        gaaacaatt tattcggaa cttgaaaaa tcggtgaat 
        aaagaagcc cacttggaa ctaaatgtt gtcattgga 
        gaaaaagct tcaagacac gttttattg gagatacga 
        aggattttg attaaagcc attcgtgac gaaagtgaa 
        aatgataat agtggtcac tcgagctta gaaaacggc 
        caccaacac tcaaaagaa catggaaaa gttaaacaa 
        aacggaaaa agccactaa
      mRNA sequence
      • >TC032285 RA
        atttatgta taaattcag caaatgcct aataactca 
        caaatgatt acaatgaag cgaaaagca gtggtgttt 
        tgtgccttg ttattgagc acttttgga cacgagctt 
        ttaaatcgt caaaaacca tcccatgct cttttatcg 
        cgtgtccat tctaaagaa gaatgccgt ctcgaatgc 
        ataccagat tcaggaaac tcatcgaat ttgcaaatt 
        tttatcaac acaactaca aatttagtg gctgtggct 
        agtactaaa accgaagtt gcgttaatt ctggatgta 
        aattcggga ttggtcggt ctgtttaat gcaatttct 
        cattcggtg ataataatt aatattgaa cgaagcacc 
        agagttgca ttttctcaa aattctaca tggaacaag 
        tctgaaact ttccaacaa tactttcaa actctaact 
        tcgttaaaa attttatac gaacaaggg gaacatcaa 
        ggcgaaaag ttttccgat agtaatatt acacaagaa 
        attgcgata caaataact ttcgtttta aaaattcta 
        acgtctgat aaatattcg attcaagtt aaagaagca 
        ttagaggtg cttgttgat gtttcaaaa aatttgcta 
        aaatctttg agagaagtc accgaaatt gtaaaaagt 
        caagttatt aatacaaac cttgaaata aaagaatta 
        attggaatt gtgaacggt ttagctagt tttgtaaaa 
        cgcctaaac ggaggcatt tctctttta acaaaacga 
        gaaataaga ctcgacttg gacattatt ataaattct 
        aaaaccgca atagatgtt ttcctttac attttacta 
        caactgaaa tcgagtaaa ttacgtttg aatgaagtt 
        ctacgtgat gggtttgac tcacctcaa ggctttttt 
        ttcctaatt gcaaaatta ggaggaaat tctacacgc 
        ggcatcaat gggattttg acaatactt tcaaaaata 
        atcgctttg aatcaacga acttccatt gagtctgtt 
        gttatcgct ctgatacaa ttttttggt aattcaaat 
        ggcaaaaat tttggtaaa gaatggcaa actacgatc 
        gaaaattta tcacgtact attaaacaa cttcacttg 
        aatgatatt ctaaaaaaa gtcgtgaaa caactccag 
        gatattcgc aacgaaaaa tggaccgaa aatcacgtt 
        aacttatcc aaaatcgtt aaaatttta actaatgct 
        tcaacccca attgattca attgttaaa attcttgca 
        attcttcaa aaagttgtt ccgattaat gtttcgctc 
        cctttcttg ttaatcgaa tttgtgcgt cttttaacg 
        acgaaaact ggacttgaa cttattgtt gctttaata 
        ccgcaaatt aaaaccaac acgaaaact tcaaagagt 
        gaaattctt cgaagcatt ttgcgtgct attgatatc 
        aaaaattcg ggtaatatc ggtaaagca ttaaaaaat 
        gtaccaata gttgaaaca atttattcg gaacttgaa 
        aaatcggtg aataaagaa gcccacttg gaactaaat 
        gttgtcatt ggagaaaaa gcttcaaga cacgtttta 
        ttggagata cgaaggatt ttgattaaa gccattcgt 
        gacgaaagt gaaaatgat aatagtggt cactcgagc 
        ttagaaaac ggccaccaa cactcaaaa gaacatgga 
        aaagttaaa caaaacgga aaaagccac taatttaca 
        atacaaaaa tgtaaaaat gtaataaat aatgaatga 
        gtac