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Gene information
General information
TC003216 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid13: 54578..59744
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003216
    iBeetle screen iB_00536
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003216 RA
      MAFDRRLFN VVLLGLAFM LVFTAFQTM GNIQKTIIE 
      SIKNDNLKK NDTSFDGDG YVSLSIIYA VMSILNWAA 
      PSVITVIGP NFAMLFGGI TYVLFIMSF LIPRTWLLY 
      FASVVIGAG AAVIWTGQG NYLTLNSTQ ATVSRNSGI 
      FWAMLQLSM FIGNLFVYF KFKGLDSID EDTRNVVIW 
      TLSGIGIAG LVVMVLLPR PKKTSQIDI PEEVQQTPI 
      EALKGAVKL FFTNDMLLL SITFLYTGL ELGFFSGVY 
      SSCIGFTKN FSNPKELVG LSGIFIGLG EVLGGAAFG 
      ILGSKTIKW GRDPIVIAG FIVHVISFF LIFLNLPNN 
      SPFNDTNDG AIITSNAEL AIFCSFLLG FGDSCFNTQ 
      IYSILGGVY SNNSASAFA IFKFTQSVA AAICFGYST 
      ALNLYGQLG ILLVLAICG TASFVIVEW KVKKEALAK 
      SVHDNASSE GSEKAAF
    CDS sequence
    • >TC003216 RA
      atggcattc gaccgacgt ttattcaac gtggtcctt 
      ctgggccta gctttcatg ttggttttt acggctttt 
      cagaccatg ggcaacata caaaaaacc atcattgag 
      agtataaag aacgacaat ttaaaaaag aacgataca 
      agttttgat ggtgatggt tacgttagt ctttcaatt 
      atttacgct gtgatgtct atactaaac tgggcagct 
      ccttctgtg ataactgtg ataggtcca aactttgcc 
      atgttattc ggaggaatc acttatgta ctcttcatt 
      atgtcattc cttattcca cgaacatgg ctgctgtat 
      ttcgccagt gttgttatc ggagctgga gctgcagtt 
      atttggact ggacaaggc aactacctc acattaaat 
      tccacacag gcaacggtt tcaagaaat tccggcatt 
      ttctgggca atgctacaa ctaagcatg tttatcgga 
      aatctcttc gtctatttt aaattcaaa ggtctggat 
      tcaatcgat gaagacact agaaatgta gttatttgg 
      actttgagt gggataggt attgctgga cttgtcgtt 
      atggtttta cttcctaga ccgaaaaaa acctcccaa 
      attgatatt ccggaagaa gtccagcaa acacccatc 
      gaagcgtta aaaggcgca gtcaagttg ttcttcacc 
      aatgatatg ctccttctt tccatcaca ttcttgtac 
      acaggtctc gaactgggt ttctttagc ggagtctac 
      agctcatgt atcggcttc accaaaaat ttctccaac 
      cccaaagaa ctagttggc ctttctggc attttcatt 
      ggtcttggt gaagtccta ggtggggct gcttttggt 
      attttgggt tccaagacc atcaaatgg ggtcgtgat 
      ccaatcgtt atcgctgga tttattgta cacgttatc 
      agctttttc ctcattttc ctcaacttg cccaacaat 
      tctcccttc aacgacact aatgatggg gctattatt 
      acaagtaat gccgaattg gccatcttc tgttcgttc 
      cttcttggt tttggagat tcgtgcttc aacacgcaa 
      atttattca attttggga ggagtttat tcaaataat 
      agcgcctca gcgtttgct atcttcaaa ttcacacaa 
      tctgtagca gctgcaatt tgtttcgga tattctacc 
      gcacttaat ttgtacggt cagttgggg attctcctc 
      gttttggcg atttgtggc accgctagt tttgttata 
      gttgagtgg aaggtaaag aaggaagcg cttgcgaaa 
      agtgttcac gataatgcg agcagtgaa gggagcgaa 
      aaagctgcc ttttaa
    mRNA sequence
    • >TC003216 RA
      aaccagaca cacaatcag aacgaaagt gaatgtttc 
      aagttaaat tttcatctt ttccagttt aaaagaaag 
      agagaatgg cattcgacc gacgtttat tcaacgtgg 
      tccttctgg gcctagctt tcatgttgg tttttacgg 
      cttttcaga ccatgggca acatacaaa aaaccatca 
      ttgagagta taaagaacg acaatttaa aaaagaacg 
      atacaagtt ttgatggtg atggttacg ttagtcttt 
      caattattt acgctgtga tgtctatac taaactggg 
      cagctcctt ctgtgataa ctgtgatag gtccaaact 
      ttgccatgt tattcggag gaatcactt atgtactct 
      tcattatgt cattcctta ttccacgaa catggctgc 
      tgtatttcg ccagtgttg ttatcggag ctggagctg 
      cagttattt ggactggac aaggcaact acctcacat 
      taaattcca cacaggcaa cggtttcaa gaaattccg 
      gcattttct gggcaatgc tacaactaa gcatgttta 
      tcggaaatc tcttcgtct attttaaat tcaaaggtc 
      tggattcaa tcgatgaag acactagaa atgtagtta 
      tttggactt tgagtggga taggtattg ctggacttg 
      tcgttatgg ttttacttc ctagaccga aaaaaacct 
      cccaaattg atattccgg aagaagtcc agcaaacac 
      ccatcgaag cgttaaaag gcgcagtca agttgttct 
      tcaccaatg atatgctcc ttctttcca tcacattct 
      tgtacacag gtctcgaac tgggtttct ttagcggag 
      tctacagct catgtatcg gcttcacca aaaatttct 
      ccaacccca aagaactag ttggccttt ctggcattt 
      tcattggtc ttggtgaag tcctaggtg gggctgctt 
      ttggtattt tgggttcca agaccatca aatggggtc 
      gtgatccaa tcgttatcg ctggattta ttgtacacg 
      ttatcagct ttttcctca ttttcctca acttgccca 
      acaattctc ccttcaacg acactaatg atggggcta 
      ttattacaa gtaatgccg aattggcca tcttctgtt 
      cgttccttc ttggttttg gagattcgt gcttcaaca 
      cgcaaattt attcaattt tgggaggag tttattcaa 
      ataatagcg cctcagcgt ttgctatct tcaaattca 
      cacaatctg tagcagctg caatttgtt tcggatatt 
      ctaccgcac ttaatttgt acggtcagt tggggattc 
      tcctcgttt tggcgattt gtggcaccg ctagttttg 
      ttatagttg agtggaagg taaagaagg aagcgcttg 
      cgaaaagtg ttcacgata atgcgagca gtgaaggga 
      gcgaaaaag ctgcctttt aagtattta tttatcttt 
      attcatacg taaaatttc tccgttcgt tacaagtat 
      tgcttctga gaagctttt acgtagtac aaatggtaa 
      caaacaaag tttccttta ataatctgt gatgaaaca 
      aagtgagac tgaggaatc aattgtatt ttgttgcca 
      tttctttaa attataata tattgattg ttaataact 
      cctgtaaat attattatg tacatttta gtgtgtcac 
      agaggttta ttatgtaaa tatattttt atcaaatct 
      gtgataaca atcaggttt tggcaattt taacgtaag 
      agattttga aacaaagga acaaatcgt tgttggctt 
      taaatttac aataaaaat tattcaata tt