General information | |||
TC003213 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 79203..85434 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs | TC003213 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_00535 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
- >TC003213 RA
MTDLIVSRG DTFPRSFLD TYRASRTSE QLYNDLKSS KSDELVLQN PADIPIAPE IHHGHSENL LDASGCLSP TENDFLESS EMILRSNRR KVRPDYDRV DPEDSIRDI VTGNDFYRF VLFKKHYDK YVHLSQKYE EARNIAYYL EEKYHEVKM ERDDLIQQR DMLSKRLES NESLLREKE DEVFLQFER VVYLEEQCD KLKAEKEKF HQQKLAIEK EKEAAYRLL TEQARESEH TRRKLERAR QEVFHHMTR MKYEKESLE RENDQLKEA LEAERKGMR KYLNQMHQR KKQVDSLEG QLTDRQNTP LASQYQKAL QHLATCKKC SVCSYAKTA YRKNSRKYD RKLLGCLQT PFMEMRNMI KPPPSPIHG EGESLSEWF CHLNTPNPS ECSSLSVPF AELAITYMD DTSDTSSHC LRDHDVDDS SSQAACNSG KGFGSDSGF SSDICGDFK SNATTPKEK FSPNGTLDE ADCAKLTRT RWTASFRKL INRIRK
- >TC003213 RA
atgaccgat ttgatcgtt tctcgtggt gatacattc ccgcgatcc tttttggat acgtatcgt gcgagccgg accagtgag cagttgtac aatgactta aaatcaagc aaaagcgac gaattagtt ctgcaaaat ccggccgac attccaatt gcaccggaa atccatcat gggcattcg gagaattta ttggatgca tctgggtgt ttgtctccg actgagaat gacttcttg gagtcgtcg gagatgatc ttgaggagt aatagaagg aaggtcagg ccggattat gacagggtt gatccagag gatagtatc agggatatc gtcacagga aatgatttc tacagattc gtgcttttc aaaaaacac tatgataaa tacgttcac ttgtcgcaa aaatacgaa gaagccaga aacattgcc tattatctt gaagagaaa taccacgaa gtcaagatg gaaagggat gatctcatt caacagaga gacatgctg agcaaaaga ctggagtcg aacgaaagt ttattgagg gagaaagaa gacgaagtt tttcttcaa ttcgaacgt gtggtttat ttggaggaa cagtgtgat aagctaaaa gccgaaaaa gaaaaattc catcaacaa aaactggca atcgaaaag gagaaagaa gccgcttac agattactg acagaacag gcccgcgaa tcggaacat acacgtcgg aaactggaa agggcgcgt caggaagta tttcatcac atgacaaga atgaaatac gaaaaagaa agcttggaa cgagagaac gatcaactg aaagaggcc ttagaagcg gaacgtaaa ggtatgagg aagtatcta aaccagatg catcaaagg aaaaaacaa gtggacagt ctggaagga cagcttacg gatcgtcag aacacaccg ttggcttcg caataccaa aaggccctt caacatctt gccacttgt aagaaatgc tcggtctgt tcgtacgcc aaaacggcc tacaggaag aacagtcgc aaatacgat cgtaaactt ttgggttgt ctccaaaca ccgtttatg gaaatgcgc aatatgatc aaaccgcct ccttcaccc attcatggt gaaggcgaa agtctttct gagtggttt tgccacctt aatacaccc aatcctagc gaatgttcc tcgttgagt gtccctttt gctgaactg gccattacc tatatggac gatacttca gatacttca tcacactgt ctcagagat catgacgtg gacgattcg tcgagtcaa gcggcttgt aatagcggc aaaggtttc gggagcgat tcaggtttt tcgtccgac atttgcggc gattttaag agtaacgca acaacgcca aaagagaag tttagtccg aacggaact ttggatgaa gcggactgt gccaaatta actcgtaca agatggacc gcttcattt aggaagcta atcaatcgt attagaaag tag
- >TC003213 RA
cgtaattga ataatgacc gatttgatc gtttctcgt ggtgataca ttcccgcga tcctttttg gatacgtat cgtgcgagc cggaccagt gagcagttg tacaatgac ttaaaatca agcaaaagc gacgaatta gttctgcaa aatccggcc gacattcca attgcaccg gaaatccat catgggcat tcggagaat ttattggat gcatctggg tgtttgtct ccgactgag aatgacttc ttggagtcg tcggagatg atcttgagg agtaataga aggaaggtc aggccggat tatgacagg gttgatcca gaggatagt atcagggat atcgtcaca ggaaatgat ttctacaga ttcgtgctt ttcaaaaaa cactatgat aaatacgtt cacttgtcg caaaaatac gaagaagcc agaaacatt gcctattat cttgaagag aaataccac gaagtcaag atggaaagg gatgatctc attcaacag agagacatg ctgagcaaa agactggag tcgaacgaa agtttattg agggagaaa gaagacgaa gtttttctt caattcgaa cgtgtggtt tatttggag gaacagtgt gataagcta aaagccgaa aaagaaaaa ttccatcaa caaaaactg gcaatcgaa aaggagaaa gaagccgct tacagatta ctgacagaa caggcccgc gaatcggaa catacacgt cggaaactg gaaagggcg cgtcaggaa gtatttcat cacatgaca agaatgaaa tacgaaaaa gaaagcttg gaacgagag aacgatcaa ctgaaagag gccttagaa gcggaacgt aaaggtatg aggaagtat ctaaaccag atgcatcaa aggaaaaaa caagtggac agtctggaa ggacagctt acggatcgt cagaacaca ccgttggct tcgcaatac caaaaggcc cttcaacat cttgccact tgtaagaaa tgctcggtc tgttcgtac gccaaaacg gcctacagg aagaacagt cgcaaatac gatcgtaaa cttttgggt tgtctccaa acaccgttt atggaaatg cgcaatatg atcaaaccg cctccttca cccattcat ggtgaaggc gaaagtctt tctgagtgg ttttgccac cttaataca cccaatcct agcgaatgt tcctcgttg agtgtccct tttgctgaa ctggccatt acctatatg gacgatact tcagatact tcatcacac tgtctcaga gatcatgac gtggacgat tcgtcgagt caagcggct tgtaatagc ggcaaaggt ttcgggagc gattcaggt ttttcgtcc gacatttgc ggcgatttt aagagtaac gcaacaacg ccaaaagag aagtttagt ccgaacgga actttggat gaagcggac tgtgccaaa ttaactcgt acaagatgg accgcttca tttaggaag ctaatcaat cgtattaga aagtagact gatgaagaa aaataaaag cacaaattg aaaacaata gtaggtgtt aacggttta taagacttg atagatcga atatcaaat gttttttcg aaatttatt aaatcacgt tttgtgtct aattaaata cgacgtatc tcctgtttt ttcatgtaa caggttttt tcgtagggg aattatata atactccgc ccgtaggca ccaacattt tatagttaa tgttaaaag tgaatgtta aaaatatat acaaagtga aacacctat ttctacttt gttagacga tgaaatcat tttacaaat ttgttgaaa tcaatttga gtattgagt gctatttta aagtattta caagtttgt tcttgcctt ctaaatgtt ttaagtgcc gtccaagta ttttttaaa gctgtttat gatttttaa gtattactt ttttcattt atttaattg gtggcagtc ttagtttta atgactatt tatttcaac cattccaat attacgatt acataagta gaaattcat taagtgtga acacataat tgcatctaa cgtttttca ggtgcagat tggcagcat ttgtgataa aataaaaca atttttgtc tg
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
