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Gene information
General information
TC003213 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid13: 79203..85434
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003213
    iBeetle screen iB_00535
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003213 RA
      MTDLIVSRG DTFPRSFLD TYRASRTSE QLYNDLKSS 
      KSDELVLQN PADIPIAPE IHHGHSENL LDASGCLSP 
      TENDFLESS EMILRSNRR KVRPDYDRV DPEDSIRDI 
      VTGNDFYRF VLFKKHYDK YVHLSQKYE EARNIAYYL 
      EEKYHEVKM ERDDLIQQR DMLSKRLES NESLLREKE 
      DEVFLQFER VVYLEEQCD KLKAEKEKF HQQKLAIEK 
      EKEAAYRLL TEQARESEH TRRKLERAR QEVFHHMTR 
      MKYEKESLE RENDQLKEA LEAERKGMR KYLNQMHQR 
      KKQVDSLEG QLTDRQNTP LASQYQKAL QHLATCKKC 
      SVCSYAKTA YRKNSRKYD RKLLGCLQT PFMEMRNMI 
      KPPPSPIHG EGESLSEWF CHLNTPNPS ECSSLSVPF 
      AELAITYMD DTSDTSSHC LRDHDVDDS SSQAACNSG 
      KGFGSDSGF SSDICGDFK SNATTPKEK FSPNGTLDE 
      ADCAKLTRT RWTASFRKL INRIRK
    CDS sequence
    • >TC003213 RA
      atgaccgat ttgatcgtt tctcgtggt gatacattc 
      ccgcgatcc tttttggat acgtatcgt gcgagccgg 
      accagtgag cagttgtac aatgactta aaatcaagc 
      aaaagcgac gaattagtt ctgcaaaat ccggccgac 
      attccaatt gcaccggaa atccatcat gggcattcg 
      gagaattta ttggatgca tctgggtgt ttgtctccg 
      actgagaat gacttcttg gagtcgtcg gagatgatc 
      ttgaggagt aatagaagg aaggtcagg ccggattat 
      gacagggtt gatccagag gatagtatc agggatatc 
      gtcacagga aatgatttc tacagattc gtgcttttc 
      aaaaaacac tatgataaa tacgttcac ttgtcgcaa 
      aaatacgaa gaagccaga aacattgcc tattatctt 
      gaagagaaa taccacgaa gtcaagatg gaaagggat 
      gatctcatt caacagaga gacatgctg agcaaaaga 
      ctggagtcg aacgaaagt ttattgagg gagaaagaa 
      gacgaagtt tttcttcaa ttcgaacgt gtggtttat 
      ttggaggaa cagtgtgat aagctaaaa gccgaaaaa 
      gaaaaattc catcaacaa aaactggca atcgaaaag 
      gagaaagaa gccgcttac agattactg acagaacag 
      gcccgcgaa tcggaacat acacgtcgg aaactggaa 
      agggcgcgt caggaagta tttcatcac atgacaaga 
      atgaaatac gaaaaagaa agcttggaa cgagagaac 
      gatcaactg aaagaggcc ttagaagcg gaacgtaaa 
      ggtatgagg aagtatcta aaccagatg catcaaagg 
      aaaaaacaa gtggacagt ctggaagga cagcttacg 
      gatcgtcag aacacaccg ttggcttcg caataccaa 
      aaggccctt caacatctt gccacttgt aagaaatgc 
      tcggtctgt tcgtacgcc aaaacggcc tacaggaag 
      aacagtcgc aaatacgat cgtaaactt ttgggttgt 
      ctccaaaca ccgtttatg gaaatgcgc aatatgatc 
      aaaccgcct ccttcaccc attcatggt gaaggcgaa 
      agtctttct gagtggttt tgccacctt aatacaccc 
      aatcctagc gaatgttcc tcgttgagt gtccctttt 
      gctgaactg gccattacc tatatggac gatacttca 
      gatacttca tcacactgt ctcagagat catgacgtg 
      gacgattcg tcgagtcaa gcggcttgt aatagcggc 
      aaaggtttc gggagcgat tcaggtttt tcgtccgac 
      atttgcggc gattttaag agtaacgca acaacgcca 
      aaagagaag tttagtccg aacggaact ttggatgaa 
      gcggactgt gccaaatta actcgtaca agatggacc 
      gcttcattt aggaagcta atcaatcgt attagaaag 
      tag
    mRNA sequence
    • >TC003213 RA
      cgtaattga ataatgacc gatttgatc gtttctcgt 
      ggtgataca ttcccgcga tcctttttg gatacgtat 
      cgtgcgagc cggaccagt gagcagttg tacaatgac 
      ttaaaatca agcaaaagc gacgaatta gttctgcaa 
      aatccggcc gacattcca attgcaccg gaaatccat 
      catgggcat tcggagaat ttattggat gcatctggg 
      tgtttgtct ccgactgag aatgacttc ttggagtcg 
      tcggagatg atcttgagg agtaataga aggaaggtc 
      aggccggat tatgacagg gttgatcca gaggatagt 
      atcagggat atcgtcaca ggaaatgat ttctacaga 
      ttcgtgctt ttcaaaaaa cactatgat aaatacgtt 
      cacttgtcg caaaaatac gaagaagcc agaaacatt 
      gcctattat cttgaagag aaataccac gaagtcaag 
      atggaaagg gatgatctc attcaacag agagacatg 
      ctgagcaaa agactggag tcgaacgaa agtttattg 
      agggagaaa gaagacgaa gtttttctt caattcgaa 
      cgtgtggtt tatttggag gaacagtgt gataagcta 
      aaagccgaa aaagaaaaa ttccatcaa caaaaactg 
      gcaatcgaa aaggagaaa gaagccgct tacagatta 
      ctgacagaa caggcccgc gaatcggaa catacacgt 
      cggaaactg gaaagggcg cgtcaggaa gtatttcat 
      cacatgaca agaatgaaa tacgaaaaa gaaagcttg 
      gaacgagag aacgatcaa ctgaaagag gccttagaa 
      gcggaacgt aaaggtatg aggaagtat ctaaaccag 
      atgcatcaa aggaaaaaa caagtggac agtctggaa 
      ggacagctt acggatcgt cagaacaca ccgttggct 
      tcgcaatac caaaaggcc cttcaacat cttgccact 
      tgtaagaaa tgctcggtc tgttcgtac gccaaaacg 
      gcctacagg aagaacagt cgcaaatac gatcgtaaa 
      cttttgggt tgtctccaa acaccgttt atggaaatg 
      cgcaatatg atcaaaccg cctccttca cccattcat 
      ggtgaaggc gaaagtctt tctgagtgg ttttgccac 
      cttaataca cccaatcct agcgaatgt tcctcgttg 
      agtgtccct tttgctgaa ctggccatt acctatatg 
      gacgatact tcagatact tcatcacac tgtctcaga 
      gatcatgac gtggacgat tcgtcgagt caagcggct 
      tgtaatagc ggcaaaggt ttcgggagc gattcaggt 
      ttttcgtcc gacatttgc ggcgatttt aagagtaac 
      gcaacaacg ccaaaagag aagtttagt ccgaacgga 
      actttggat gaagcggac tgtgccaaa ttaactcgt 
      acaagatgg accgcttca tttaggaag ctaatcaat 
      cgtattaga aagtagact gatgaagaa aaataaaag 
      cacaaattg aaaacaata gtaggtgtt aacggttta 
      taagacttg atagatcga atatcaaat gttttttcg 
      aaatttatt aaatcacgt tttgtgtct aattaaata 
      cgacgtatc tcctgtttt ttcatgtaa caggttttt 
      tcgtagggg aattatata atactccgc ccgtaggca 
      ccaacattt tatagttaa tgttaaaag tgaatgtta 
      aaaatatat acaaagtga aacacctat ttctacttt 
      gttagacga tgaaatcat tttacaaat ttgttgaaa 
      tcaatttga gtattgagt gctatttta aagtattta 
      caagtttgt tcttgcctt ctaaatgtt ttaagtgcc 
      gtccaagta ttttttaaa gctgtttat gatttttaa 
      gtattactt ttttcattt atttaattg gtggcagtc 
      ttagtttta atgactatt tatttcaac cattccaat 
      attacgatt acataagta gaaattcat taagtgtga 
      acacataat tgcatctaa cgtttttca ggtgcagat 
      tggcagcat ttgtgataa aataaaaca atttttgtc 
      tg