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Gene information
General information
TC003212 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 130028..136767
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003212
    iBeetle screen iB_00534

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003212 RA
      MEIRKLIEL LRATIDPNQ RQQAEAQLE QIHKIIGFA 
      PSLLSVVMM ADCDMPVRQ AGAIYLKNL ISQSWQDRE 
      VEGGQPLPF AIHEQDRAL IRDSIVDAV VHAPDLIRT 
      QLCTCVHNM VKHDFPGRW TQIVDKISI YLSNPDPSG 
      WHGSLLCLY ELVKNFEYK KADERGPLH EAMNLLLPQ 
      LYQLIVRIL PDASDQSVL LQKEGLKIY FALTQYMLP 
      LDLITKEAF AQWMEVCRQ VVERPVPPA ALQPDEDER 
      PDLPWWKCK KWALHILYR MFERYGSPG LVTKEYNEF 
      AEWYLQTFS AGILEVLLR QLDGYRSGH WVPPRVLQQ 
      TLNYLNQAV SHAYTWRIL KPHMPAIIQ DVLFPLMSY 
      SPEDHELWT VDPHEYIRV KFDVFEDFV SPVTAAQTL 
      LHSSCKKRK DMLQKAMTM ITQVLTNPA TEPPQKDGA 
      LHMVGSLAD VLLRKKFYR EQLDQLFIK YVFPEFNSD 
      RGHMRARAC WVLHYFAEF PFRQENVLM EAVNLTVRA 
      LLHDTDLPV KVEAAIALQ SLLNYQDKS QKYVEPQVK 
      QVAHELLTI IRETENEDV TGVMQKLVC VYTQQLAPI 
      AVEICQHLT ATFNQVLDT DEGSDEKAI TAMGLLNTI 
      ETLLTVMEE QPEIMMLLE PTVLQVVVH VLQNEVKEF 
      YEEVLALIY DLTSKHISQ DMWKVFELL YQVFMKNGL 
      DHFTDMMPA LHNYITIDT PAFLSNEQR LLAIYNMCK 
      EVLNNDCGE DAESHAVKL LEVILLQCQ GKVDQAVPM 
      LVELAASRL LREVKTSEL RTMCLQVLI AALYYNPGL 
      LLSVLEKMP NFTENFVKQ WLHDTDCFL GIHDRKLCV 
      LGLCTLITM DNKPNCLVE MVPRVIPSL ILLFDGLKR 
      AYAAKAQEQ AEEEESESS DGEIEGEVL SSDEDEIDD 
      QGQEYLENI SRRAITVGN AAGMAISGT INDIDDESD 
      DDDSDFEPN EETMLEAYT TPLDEENCP VDEYDVFKE 
      VMTRLERCQ PEWYNALTA NLTEQQKKN LVDICVLAD 
      QRKAAKESK RIEQSGGFM FTQHEVPTT FKFGAPKPS
    CDS sequence
    • >TC003212 RA
      atggagatt cgtaagctg attgagttg ctgagggcg 
      accatcgac cccaaccag cgccagcag gccgaagcc 
      cagttggag cagatccac aagatcatc ggtttcgct 
      ccctcgttg ctttcggtg gtgatgatg gccgattgc 
      gatatgccg gtgcgacag gcgggggcc atctacttg 
      aaaaatctc atctcgcag agctggcag gatagggag 
      gttgagggc ggacagcca ctgcctttc gccatacat 
      gagcaggac agggccctc atcagagac agtatcgtg 
      gatgcggtg gtgcatgca cccgatctc attcgcacg 
      cagttgtgc acttgcgtt cataatatg gtcaagcat 
      gactttcca ggcaggtgg actcagatt gtggacaag 
      atcagcatt tacttatcg aatcctgac cccagcgga 
      tggcacgga tcccttctc tgtctttac gagctagtg 
      aaaaatttc gaatataaa aaggccgac gaaagaggg 
      cctttacac gaagctatg aacttacta ttacctcaa 
      ctttatcaa cttattgtt cgaatatta ccggacgct 
      tctgatcaa tcggtgctt ttacaaaaa gagggttta 
      aaaatttat tttgctctc acacagtac atgttacct 
      ttagatttg ataacgaaa gaggctttt gcgcaatgg 
      atggaagtc tgtcgacag gttgttgaa agaccagtt 
      ccaccagct gcactccaa ccagatgaa gatgaaagg 
      cccgattta ccatggtgg aagtgtaaa aaatgggcc 
      ttacacata ttataccgt atgtttgaa cgttacggt 
      tctcctggt ttagttacc aaggaatac aacgagttt 
      gccgaatgg tatttgcaa actttcagt gctggtatt 
      ttggaggtt cttcttaga caacttgat ggatataga 
      tcgggtcat tgggtgcct ccaagagtt ctccaacaa 
      accctaaat tatttaaat caggcagtt tcacatgct 
      tacacttgg cgtattctt aaaccacac atgccggca 
      attatacaa gatgttctt ttcccttta atgagttac 
      tctccagaa gatcatgaa ttatggacc gttgatcca 
      cacgaatac attcgagta aaattcgat gtttttgaa 
      gattttgtt tcgccagtc actgccgca caaactctg 
      ttacattca tcgtgcaaa aaacgcaaa gatatgtta 
      cagaaagca atgactatg attacacaa gtcttgact 
      aatccggca acagaacca ccacaaaaa gacggtgct 
      ttacatatg gttggttcg ttggctgat gttctacta 
      agaaagaaa ttctacagg gaacaactc gatcaactg 
      ttcattaaa tatgttttt cctgaattt aacagtgat 
      cgagggcat atgagagca agggcttgt tgggtattg 
      cactatttt gccgagttt cccttccgc caggagaat 
      gttttgatg gaggctgtt aatttgacg gttagggct 
      ttgctccat gataccgac cttccagtc aaggtggag 
      gcggctatt gcacttcaa tccctcttg aattatcaa 
      gacaaatca caaaagtat gtcgaacca caagtcaaa 
      caagtcgcc catgaatta ctgacgata attcgtgaa 
      acagaaaac gaagatgtg actggagtt atgcaaaaa 
      ttagtttgc gtttatact caacaatta gctccgata 
      gctgttgag atttgtcaa catttaacg gccactttc 
      aatcaagtt ttggatact gatgaaggt tcagatgag 
      aaagcaata acagcgatg ggtttatta aacactatt 
      gaaaccctt ttgactgtt atggaagaa caaccagaa 
      ataatgatg ttgttggaa ccgactgtc cttcaagtt 
      gttgttcac gtgttacaa aatgaagtt aaagagttc 
      tatgaggag gttttagcc ctgatttat gatttaaca 
      agtaaacac atatcgcaa gatatgtgg aaagtgttt 
      gaattgttg tatcaggtg tttatgaag aacggtctt 
      gatcatttt acagatatg atgccagcg ttacacaat 
      tatataaca atcgataca ccggccttt ctcagtaac 
      gaacaacgt cttctcgct atttacaac atgtgtaaa 
      gaagtgttg aacaacgat tgtggcgaa gatgccgaa 
      tcacatgct gttaaatta ctcgaagtg attttactg 
      caatgtcaa ggcaaagtc gatcaagct gtaccaatg 
      ttggtcgaa ttggcagca tctcgatta ttacgtgaa 
      gtcaaaacg agtgaattg cgaacaatg tgtctgcaa 
      gttttgata gctgcactt tattacaat ccgggattg 
      ttgctttcg gttcttgaa aaaatgccg aattttacc 
      gagaatttt gtcaaacag tggttgcac gatacggac 
      tgttttctc ggaatacac gataggaaa ttgtgcgtt 
      ttaggactg tgcacttta atcacgatg gataataaa 
      ccgaattgt ttggtagaa atggtgcct cgtgtgata 
      ccttcgttg attttgtta tttgatggc ttgaagcga 
      gcttatgcg gccaaagcg caagaacaa gccgaagaa 
      gaggagagc gaaagttca gatggggaa attgaagga 
      gaagttttg agttcggac gaggacgag attgatgat 
      caaggtcag gaatatttg gaaaatatc tcacgaaga 
      gccataaca gtgggaaat gctgccgga atggcaatc 
      tcaggcaca atcaatgac attgatgac gagtccgac 
      gatgacgat tctgatttc gaaccgaat gaagaaacg 
      atgttggaa gcctataca acaccatta gatgaagaa 
      aattgtcct gtagatgaa tacgatgtt ttcaaagaa 
      gtcatgact agattggaa cgttgtcag cctgaatgg 
      tataatgcc ctcacggcc aatttaacc gaacaacaa 
      aagaagaat ttggtagat atttgtgtg ttagcggat 
      cagaggaaa gcagccaaa gaaagtaaa agaattgaa 
      caatcggga ggttttatg tttacacaa catgaagta 
      cccacaact tttaagttt ggcgctcca aaaccgtca 
      tga
    mRNA sequence
    • >TC003212 RA
      caaatgtca aatcatctt gtgcggggt gagtgacgg 
      ccgaaaaaa ttctttcac cccggacgt aataaagta 
      aaatttaag tgcaagaaa tggagattc gtaagctga 
      ttgagttgc tgagggcga ccatcgacc ccaaccagc 
      gccagcagg ccgaagccc agttggagc agatccaca 
      agatcatcg gtttcgctc cctcgttgc tttcggtgg 
      tgatgatgg ccgattgcg atatgccgg tgcgacagg 
      cgggggcca tctacttga aaaatctca tctcgcaga 
      gctggcagg atagggagg ttgagggcg gacagccac 
      tgcctttcg ccatacatg agcaggaca gggccctca 
      tcagagaca gtatcgtgg atgcggtgg tgcatgcac 
      ccgatctca ttcgcacgc agttgtgca cttgcgttc 
      ataatatgg tcaagcatg actttccag gcaggtgga 
      ctcagattg tggacaaga tcagcattt acttatcga 
      atcctgacc ccagcggat ggcacggat cccttctct 
      gtctttacg agctagtga aaaatttcg aatataaaa 
      aggccgacg aaagagggc ctttacacg aagctatga 
      acttactat tacctcaac tttatcaac ttattgttc 
      gaatattac cggacgctt ctgatcaat cggtgcttt 
      tacaaaaag agggtttaa aaatttatt ttgctctca 
      cacagtaca tgttacctt tagatttga taacgaaag 
      aggcttttg cgcaatgga tggaagtct gtcgacagg 
      ttgttgaaa gaccagttc caccagctg cactccaac 
      cagatgaag atgaaaggc ccgatttac catggtgga 
      agtgtaaaa aatgggcct tacacatat tataccgta 
      tgtttgaac gttacggtt ctcctggtt tagttacca 
      aggaataca acgagtttg ccgaatggt atttgcaaa 
      ctttcagtg ctggtattt tggaggttc ttcttagac 
      aacttgatg gatatagat cgggtcatt gggtgcctc 
      caagagttc tccaacaaa ccctaaatt atttaaatc 
      aggcagttt cacatgctt acacttggc gtattctta 
      aaccacaca tgccggcaa ttatacaag atgttcttt 
      tccctttaa tgagttact ctccagaag atcatgaat 
      tatggaccg ttgatccac acgaataca ttcgagtaa 
      aattcgatg tttttgaag attttgttt cgccagtca 
      ctgccgcac aaactctgt tacattcat cgtgcaaaa 
      aacgcaaag atatgttac agaaagcaa tgactatga 
      ttacacaag tcttgacta atccggcaa cagaaccac 
      cacaaaaag acggtgctt tacatatgg ttggttcgt 
      tggctgatg ttctactaa gaaagaaat tctacaggg 
      aacaactcg atcaactgt tcattaaat atgtttttc 
      ctgaattta acagtgatc gagggcata tgagagcaa 
      gggcttgtt gggtattgc actattttg ccgagtttc 
      ccttccgcc aggagaatg ttttgatgg aggctgtta 
      atttgacgg ttagggctt tgctccatg ataccgacc 
      ttccagtca aggtggagg cggctattg cacttcaat 
      ccctcttga attatcaag acaaatcac aaaagtatg 
      tcgaaccac aagtcaaac aagtcgccc atgaattac 
      tgacgataa ttcgtgaaa cagaaaacg aagatgtga 
      ctggagtta tgcaaaaat tagtttgcg tttatactc 
      aacaattag ctccgatag ctgttgaga tttgtcaac 
      atttaacgg ccactttca atcaagttt tggatactg 
      atgaaggtt cagatgaga aagcaataa cagcgatgg 
      gtttattaa acactattg aaacccttt tgactgtta 
      tggaagaac aaccagaaa taatgatgt tgttggaac 
      cgactgtcc ttcaagttg ttgttcacg tgttacaaa 
      atgaagtta aagagttct atgaggagg ttttagccc 
      tgatttatg atttaacaa gtaaacaca tatcgcaag 
      atatgtgga aagtgtttg aattgttgt atcaggtgt 
      ttatgaaga acggtcttg atcatttta cagatatga 
      tgccagcgt tacacaatt atataacaa tcgatacac 
      cggcctttc tcagtaacg aacaacgtc ttctcgcta 
      tttacaaca tgtgtaaag aagtgttga acaacgatt 
      gtggcgaag atgccgaat cacatgctg ttaaattac 
      tcgaagtga ttttactgc aatgtcaag gcaaagtcg 
      atcaagctg taccaatgt tggtcgaat tggcagcat 
      ctcgattat tacgtgaag tcaaaacga gtgaattgc 
      gaacaatgt gtctgcaag ttttgatag ctgcacttt 
      attacaatc cgggattgt tgctttcgg ttcttgaaa 
      aaatgccga attttaccg agaattttg tcaaacagt 
      ggttgcacg atacggact gttttctcg gaatacacg 
      ataggaaat tgtgcgttt taggactgt gcactttaa 
      tcacgatgg ataataaac cgaattgtt tggtagaaa 
      tggtgcctc gtgtgatac cttcgttga ttttgttat 
      ttgatggct tgaagcgag cttatgcgg ccaaagcgc 
      aagaacaag ccgaagaag aggagagcg aaagttcag 
      atggggaaa ttgaaggag aagttttga gttcggacg 
      aggacgaga ttgatgatc aaggtcagg aatatttgg 
      aaaatatct cacgaagag ccataacag tgggaaatg 
      ctgccggaa tggcaatct caggcacaa tcaatgaca 
      ttgatgacg agtccgacg atgacgatt ctgatttcg 
      aaccgaatg aagaaacga tgttggaag cctatacaa 
      caccattag atgaagaaa attgtcctg tagatgaat 
      acgatgttt tcaaagaag tcatgacta gattggaac 
      gttgtcagc ctgaatggt ataatgccc tcacggcca 
      atttaaccg aacaacaaa agaagaatt tggtagata 
      tttgtgtgt tagcggatc agaggaaag cagccaaag 
      aaagtaaaa gaattgaac aatcgggag gttttatgt 
      ttacacaac atgaagtac ccacaactt ttaagtttg 
      gcgctccaa aaccgtcat gaatatatg tgacgtggt 
      gtgtaaaat ttttgtgac tgaatgtaa aattgactg 
      ttaagacca gtgaaatga tgtacagtt ggtaattta 
      atctttttt aatttttat taagtgcct ttttattaa 
      acaaaaaaa aaacggatg taaacgatt taaatttac 
      ctaatatag atttaaatg gaagtttat tttccgtgt 
      aattgtaat tatttttcg actgtgtct cttttgtct 
      catttgttt tcttcttta gggaaagac aatgtagta 
      aattacatt atttctata atatactga aaggtgtta 
      a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY