Status
Please wait ...
Gene information
General information
TC003211 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 137273..140077
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003211
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00533

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003211 RA
      MKVSENEKE NVLSRRLNK ILETRLEND QDTLEALKQ 
      LSTFYTENT LQARRNLRS QIEKRSLDI NENFLASFR 
      EVKLCFDAV YNDVSEMSK SIQEMTHRL HNAKMQTKQ 
      LLEQTKSLQ ESGEKNDMQ QQIVHAFLE TFQLTPDEL 
      VALHGNKQK RDAPLTNDI FSALDHIQK IHSDCKVLM 
      QSGHQTLAL DIMEQMTLH QEGALERLY RWTQHHCRN 
      VDNPDLTEI ITQAMARLQ ERQMLFKYI IDEYCIARR 
      SVLVGEFIN ALTRGGPSG NPAPIELRA HDPQIYITD 
      MLVWLNKAI PLEKQNLHL LVKLCDKTD LNQQLNEAL 
      ASICEGVCQ PLKIRIEKI LTSSVAPSI LYAVTNLIR 
      YYKKCICKI VTGGLLETT LVEVQERSE GAFLTTLQQ 
      QVNNILVRV EAPPRDLSP TPSINHLLA ILRDTLSTA 
      SVSEGRESD MGKIAQCIM EPLLRAVNE QASRLPPTD 
      MAVYMLNCM YSMYTCLSL YEFMDERLE RLQAQSDAQ 
      IDTLTSEQA SSLVANLNL GPIYTILQD HTQGPLSGI 
      PGMEPTNLK NFLAKLDYL LASPDSVLL PQINLLLSS 
      KHKKAVQKR AFDVLLAIY KQLYEGVHN PDNLYENPG 
      SILSKTPDE LMKMLN
    CDS sequence
    • >TC003211 RA
      atgaaagtg tcggaaaac gaaaaagaa aacgtcctt 
      tcgcgacgt ttgaataaa atcctcgag acaagactt 
      gaaaacgac caggacact ttggaagca ctaaaacaa 
      ctctcaact ttttacacc gaaaacacg cttcaagcc 
      cgtcgaaat ttacgcagt cagatcgaa aaacgcagt 
      cttgacatc aacgagaat tttttagca tcgtttaga 
      gaagtaaaa ctgtgtttt gatgccgtt tataacgac 
      gtttccgag atgagtaaa tcgattcaa gagatgaca 
      catcgacta cacaatgcc aaaatgcaa acaaagcaa 
      ttgctagaa cagaccaag tcgttacaa gaaagtggc 
      gaaaaaaac gacatgcaa caacagata gttcatgct 
      tttttggag acgtttcaa ttaacaccg gatgaactt 
      gttgcttta catggaaac aagcaaaaa cgggacgcc 
      ccgcttact aatgatatt ttttcggcg ttagatcac 
      atccagaaa attcattcc gattgcaaa gttttgatg 
      cagtcagga caccaaact ttagctcta gatatcatg 
      gaacagatg acgttgcat caagaaggg gctttggaa 
      cgactttac agatggaca caacatcac tgtcgaaat 
      gttgataat cctgattta actgaaata ataactcaa 
      gcaatggcc agattacaa gaacgtcaa atgttattt 
      aaatacata attgatgaa tattgtata gcgagacgc 
      tctgtgtta gtgggcgaa tttatcaac gctttgaca 
      cgtgggggc ccttcgggg aatcccgcc cccattgaa 
      ttaagggca cacgacccc caaatttat atcacagat 
      atgctagtt tggttgaac aaagcaata cctctggaa 
      aaacaaaat cttcatttg cttgtaaaa ttgtgcgat 
      aaaactgac ttaaaccag caactgaat gaagctttg 
      gcttcgatt tgtgaaggt gtttgtcag ccgttaaaa 
      atccggatc gaaaaaatt ctcacttct tcagttgct 
      ccatctatc ttatatgca gttacaaat ctgatccgt 
      tattacaaa aaatgcatt tgtaaaatt gttactggt 
      gggctactt gagacaaca ctggtcgaa gtgcaggaa 
      cgtagtgag ggggctttc ttaactacc ctccaacaa 
      caagtcaat aatattcta gtgagggtt gaggcgccc 
      cccagggac ttgtctcca accccttca attaaccac 
      ttattggcc attttgagg gacacattg tccacggct 
      agtgttagt gaagggcga gagagtgat atgggaaaa 
      attgcgcaa tgtattatg gagcccctt ttgagggct 
      gtcaatgaa caagcctct cgtctacct cctacagac 
      atggccgtc tatatgtta aactgcatg tattcaatg 
      tacacttgt ttgtcactt tatgaattt atggacgag 
      agattggaa cgactccaa gcacaatct gatgctcaa 
      atcgatacg ttaacctca gaacaggcc agttcttta 
      gttgcgaat cttaattta gggccgatt tataccatt 
      ttgcaagat cacacgcaa ggacccctg tcggggatt 
      cccggaatg gaacccaca aatttgaag aattttttg 
      gctaaattg gattatttg ttggcttcc cctgacagt 
      gtcttgtta ccacaaatc aacttgtta ttgtcatct 
      aaacacaaa aaagccgta caaaagagg gcgtttgat 
      gtacttttg gccatttat aaacagttg tatgaaggt 
      gtacacaac ccggacaat ttgtatgaa aatccaggc 
      agtattctg agcaagaca ccagatgaa ttaatgaaa 
      atgttaaat tag
    mRNA sequence
    • >TC003211 RA
      catgaaacg tcaaattga ggttatgtt aaaacccta 
      aataatagt tattaaatt ttttaaata atttaaata 
      cacgatgaa agtgtcgga aaacgaaaa agaaaacgt 
      cctttcgcg acgtttgaa taaaatcct cgagacaag 
      acttgaaaa cgaccagga cactttgga agcactaaa 
      acaactctc aacttttta caccgaaaa cacgcttca 
      agcccgtcg aaatttacg cagtcagat cgaaaaacg 
      cagtcttga catcaacga gaatttttt agcatcgtt 
      tagagaagt aaaactgtg ttttgatgc cgtttataa 
      cgacgtttc cgagatgag taaatcgat tcaagagat 
      gacacatcg actacacaa tgccaaaat gcaaacaaa 
      gcaattgct agaacagac caagtcgtt acaagaaag 
      tggcgaaaa aaacgacat gcaacaaca gatagttca 
      tgctttttt ggagacgtt tcaattaac accggatga 
      acttgttgc tttacatgg aaacaagca aaaacggga 
      cgccccgct tactaatga tattttttc ggcgttaga 
      tcacatcca gaaaattca ttccgattg caaagtttt 
      gatgcagtc aggacacca aactttagc tctagatat 
      catggaaca gatgacgtt gcatcaaga aggggcttt 
      ggaacgact ttacagatg gacacaaca tcactgtcg 
      aaatgttga taatcctga tttaactga aataataac 
      tcaagcaat ggccagatt acaagaacg tcaaatgtt 
      atttaaata cataattga tgaatattg tatagcgag 
      acgctctgt gttagtggg cgaatttat caacgcttt 
      gacacgtgg gggcccttc ggggaatcc cgcccccat 
      tgaattaag ggcacacga cccccaaat ttatatcac 
      agatatgct agtttggtt gaacaaagc aatacctct 
      ggaaaaaca aaatcttca tttgcttgt aaaattgtg 
      cgataaaac tgacttaaa ccagcaact gaatgaagc 
      tttggcttc gatttgtga aggtgtttg tcagccgtt 
      aaaaatccg gatcgaaaa aattctcac ttcttcagt 
      tgctccatc tatcttata tgcagttac aaatctgat 
      ccgttatta caaaaaatg catttgtaa aattgttac 
      tggtgggct acttgagac aacactggt cgaagtgca 
      ggaacgtag tgagggggc tttcttaac taccctcca 
      acaacaagt caataatat tctagtgag ggttgaggc 
      gccccccag ggacttgtc tccaacccc ttcaattaa 
      ccacttatt ggccatttt gagggacac attgtccac 
      ggctagtgt tagtgaagg gcgagagag tgatatggg 
      aaaaattgc gcaatgtat tatggagcc ccttttgag 
      ggctgtcaa tgaacaagc ctctcgtct acctcctac 
      agacatggc cgtctatat gttaaactg catgtattc 
      aatgtacac ttgtttgtc actttatga atttatgga 
      cgagagatt ggaacgact ccaagcaca atctgatgc 
      tcaaatcga tacgttaac ctcagaaca ggccagttc 
      tttagttgc gaatcttaa tttagggcc gatttatac 
      cattttgca agatcacac gcaaggacc cctgtcggg 
      gattcccgg aatggaacc cacaaattt gaagaattt 
      tttggctaa attggatta tttgttggc ttcccctga 
      cagtgtctt gttaccaca aatcaactt gttattgtc 
      atctaaaca caaaaaagc cgtacaaaa gagggcgtt 
      tgatgtact tttggccat ttataaaca gttgtatga 
      aggtgtaca caacccgga caatttgta tgaaaatcc 
      aggcagtat tctgagcaa gacaccaga tgaattaat 
      gaaaatgtt aaattagat atttaatta gaatattat 
      tgtgcacta ttgttatgt tactttaaa aacgactaa 
      ctaataaaa ttaaagttt tatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY