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Gene information
General information
TC003209 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 148520..163207
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003209
    iBeetle screen iB_00532

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    mature egg not present - Number of animals showing the phenotype: 4

    egg chamber number decreased - Number of animals showing the phenotype: 4

    tropharium potentially degenerated - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003209 RA
      MSLETRSNS ALFKRAEQL KRWEDSDTN REPVYPKQT 
      SRKIKFSSG CVFLAACAA GDKDEVLRL LENGADINT 
      ANVDGLTAL HQACIDDNS EMVEFLVEH GADVNRGDN 
      EGWTPLHAT ASCGFLYIA KYLIEKGAN VAAVNNDGE 
      LAVDIAECQ KMEDLLNEE IKKRGIDCD AARNEEKRI 
      MLQDAKEWL ATKSTLVDA PHPKNGATA LHVAAAKGY 
      TDVMKILLQ CGADIDAQD IDGWSPLHA AAHWGHKDA 
      CQILAENLA DMDAKNYVG QTPFDLADN DMLKVLEDL 
      KKKQNKEDV LNRRQAKKR AEIQDRTIE TETPSKVKK 
      VEVEAEKED KEEDNIEMD VSSESESET ESSSETESN 
      STDDEKKNR VNSLDAVSI SNKPAEPTR LPPSVPPKQ 
      LVDSNNEDN APSWRKQNT RQTKADLPS PVKTIADSV 
      NNENDVTLR RPTSLQADK EFYSKYEEL HARIKKYRN 
      SNDIIPYST RPHSDIIPN SYSTSQQHY RSLKEDSNE 
      NNSTSSTKT SPVMSPVSN NQPLRRSFV PPVRDEESE 
      TQRKAHAKR VRETRRSTQ GVTLEEIKS AEQFVKKKN 
      QPNCNNNNN EQSGANGDS PNGVSVTAT ITTAAPTVI 
      TRDTEEPSI THERRPSWR LKIDSSSKF KLEDATKSS 
      EPPSTPSFV RRTVTQNTT TNPRPSSAP VETAETTVT 
      LPLRRPKTQ DEKEQDKEN DVRNAQATL ATQAAIQRR 
      RRPKRRSTG VVSFENMDD MDQDKDPSG GGDYEDSTK 
      LNHEESDRS SRSRLGSTS DIRNENGEI DYKKLYEQA 
      LVENNELKD KLKKSDQEL RDTKQTLEK LKVVTSKNS 
      LSELEKRER RAMERKLSE MEEELKQLD IMKVENQKL 
      KDENGALIR VISKLSK
    CDS sequence
    • >TC003209 RA
      atgtcttta gagacccgt tcgaattcg gcccttttc 
      aaaagggcc gagcaattg aaacgttgg gaggactcg 
      gacacgaat cgcgagccg gtttatccg aaacaaacc 
      tcgcggaaa atcaaattt tcgtcgggt tgtgtgttc 
      cttgcagcg tgcgccgcg ggtgataaa gatgaagtc 
      ttgagactt ttggaaaac ggtgccgac ataaacact 
      gccaatgtc gatggtctt acggccctc caccaggcg 
      tgcattgac gataattcc gaaatggtc gaatttttg 
      gtcgagcac ggagcggac gtaaatcgt ggcgataac 
      gaaggatgg acaccatta catgcaaca gcaagttgc 
      ggttttttg tacattgcc aaatattta attgaaaaa 
      ggtgcaaat gttgccgcc gttaacaac gacggtgaa 
      ttggccgta gacatcgca gaatgtcaa aaaatggaa 
      gatttacta aacgaagaa atcaaaaag agaggaatt 
      gactgtgat gcagctcgc aacgaagaa aaacgtatc 
      atgttacaa gatgctaaa gaatggtta gcgaccaaa 
      agtacactt gtggacgca ccacatccc aaaaatggg 
      gctacggca cttcatgta gctgcagcc aagggatat 
      acggacgtt atgaaaatt ttgcttcaa tgtggggca 
      gatatcgac gctcaggat attgacggg tggagtcct 
      ttacatgct gcggctcat tggggacat aaggacgct 
      tgtcaaatt ctggcagaa aacctagct gatatggat 
      gcgaaaaat tatgtcggt caaactccg tttgattta 
      gccgataat gacatgttg aaggttttg gaggatctc 
      aagaagaag caaaacaaa gaggacgtg cttaatagg 
      cggcaagcg aaaaagaga gccgaaata caggatagg 
      actattgaa actgaaacg ccgagtaaa gttaaaaaa 
      gtcgaagtt gaagcagaa aaggaggat aaagaagag 
      gataatatc gaaatggac gtgtctagt gaaagtgaa 
      tccgagact gaaagcagt tctgaaaca gaatcgaac 
      agcactgat gatgagaag aagaacaga gtgaattct 
      ttagatgca gtaagcata tcaaataag cccgcagaa 
      cctacaaga ctgccacct agtgtgcct cctaaacaa 
      ttagttgat tcaaataac gaagataat gcgccctct 
      tggaggaag cagaacaca cgacagacc aaagccgat 
      ttaccaagt cctgtaaaa acaatcgcc gattctgta 
      aataatgaa aacgacgtt accctcaga agacccact 
      agtttacaa gcggataaa gagttctac tcgaaatac 
      gaggaactg catgcacgc attaagaaa tatagaaat 
      agcaatgac attattcct tattcgact agacctcat 
      tccgacatt atcccaaat tcctattca acttctcag 
      cagcattac agaagtttg aaagaagac agcaacgaa 
      aataacagt acatcatca acgaaaaca tcaccggtt 
      atgagtcca gtatctaat aatcaacca cttcgaagg 
      tcgtttgtg ccacccgtt cgtgacgaa gaaagtgaa 
      acgcaacgc aaagctcat gcgaaacgt gttcgtgag 
      acgaggcgt tcgactcaa ggtgtaaca ttggaggag 
      ataaaatcg gctgaacaa ttcgttaaa aagaagaat 
      caaccgaat tgcaataac aacaataac gagcaatct 
      ggagctaac ggggattcc cctaacgga gtgagtgtg 
      accgctaca ataaccact gcggcacct actgttata 
      acacgtgat actgaagaa cccagtatt actcatgag 
      cgtaggccg tcgtggagg ctgaaaatt gacagtagt 
      agtaagttt aaactggaa gatgcaacg aaatcctct 
      gaaccacca agcactccg tcctttgtc cgtcgcacc 
      gtcacacaa aataccacc acgaatcct agaccgtca 
      tcggcaccg gtcgaaacg gccgaaact acggtcacg 
      ttaccattg agaagacca aaaactcaa gatgaaaaa 
      gaacaagac aaagaaaat gacgtacgc aacgctcaa 
      gccactctc gccacacag gcggccatc caacgaaga 
      aggaggccc aagaggagg tccacaggg gtcgtctcg 
      tttgaaaat atggatgat atggatcaa gataaggat 
      ccgtcagga ggtggtgat tatgaagat agtacgaaa 
      ttaaatcac gaagagagt gataggtcg agcagatct 
      cgtttagga agcacttcg gatattagg aacgaaaac 
      ggcgaaatt gactataaa aaattgtat gaacaagca 
      cttgtcgaa aacaatgaa ctcaaggac aaactgaaa 
      aagtcagat caagaattg agggataca aaacagaca 
      ctggagaag ctcaaagtt gtgacgagt aaaaattcg 
      ttatcggaa ttagagaaa cgcgaacgg agggcaatg 
      gaacgaaag ttgagtgag atggaggag gaattaaag 
      cagttggat attatgaag gtagaaaat caaaagcta 
      aaggacgag aatggagcg cttattaga gtgattagt 
      aagttatcg aaatag
    mRNA sequence
    • >TC003209 RA
      aaccgagtg aactttgag gtgtttggt gaggagtgg 
      gtaatggtg gccgttgtg aaaagtgtt aaataacca 
      tgtctttag agacccgtt cgaattcgg cccttttca 
      aaagggccg agcaattga aacgttggg aggactcgg 
      acacgaatc gcgagccgg tttatccga aacaaacct 
      cgcggaaaa tcaaatttt cgtcgggtt gtgtgttcc 
      ttgcagcgt gcgccgcgg gtgataaag atgaagtct 
      tgagacttt tggaaaacg gtgccgaca taaacactg 
      ccaatgtcg atggtctta cggccctcc accaggcgt 
      gcattgacg ataattccg aaatggtcg aatttttgg 
      tcgagcacg gagcggacg taaatcgtg gcgataacg 
      aaggatgga caccattac atgcaacag caagttgcg 
      gttttttgt acattgcca aatatttaa ttgaaaaag 
      gtgcaaatg ttgccgccg ttaacaacg acggtgaat 
      tggccgtag acatcgcag aatgtcaaa aaatggaag 
      atttactaa acgaagaaa tcaaaaaga gaggaattg 
      actgtgatg cagctcgca acgaagaaa aacgtatca 
      tgttacaag atgctaaag aatggttag cgaccaaaa 
      gtacacttg tggacgcac cacatccca aaaatgggg 
      ctacggcac ttcatgtag ctgcagcca agggatata 
      cggacgtta tgaaaattt tgcttcaat gtggggcag 
      atatcgacg ctcaggata ttgacgggt ggagtcctt 
      tacatgctg cggctcatt ggggacata aggacgctt 
      gtcaaattc tggcagaaa acctagctg atatggatg 
      cgaaaaatt atgtcggtc aaactccgt ttgatttag 
      ccgataatg acatgttga aggttttgg aggatctca 
      agaagaagc aaaacaaag aggacgtgc ttaataggc 
      ggcaagcga aaaagagag ccgaaatac aggatagga 
      ctattgaaa ctgaaacgc cgagtaaag ttaaaaaag 
      tcgaagttg aagcagaaa aggaggata aagaagagg 
      ataatatcg aaatggacg tgtctagtg aaagtgaat 
      ccgagactg aaagcagtt ctgaaacag aatcgaaca 
      gcactgatg atgagaaga agaacagag tgaattctt 
      tagatgcag taagcatat caaataagc ccgcagaac 
      ctacaagac tgccaccta gtgtgcctc ctaaacaat 
      tagttgatt caaataacg aagataatg cgccctctt 
      ggaggaagc agaacacac gacagacca aagccgatt 
      taccaagtc ctgtaaaaa caatcgccg attctgtaa 
      ataatgaaa acgacgtta ccctcagaa gacccacta 
      gtttacaag cggataaag agttctact cgaaatacg 
      aggaactgc atgcacgca ttaagaaat atagaaata 
      gcaatgaca ttattcctt attcgacta gacctcatt 
      ccgacatta tcccaaatt cctattcaa cttctcagc 
      agcattaca gaagtttga aagaagaca gcaacgaaa 
      ataacagta catcatcaa cgaaaacat caccggtta 
      tgagtccag tatctaata atcaaccac ttcgaaggt 
      cgtttgtgc cacccgttc gtgacgaag aaagtgaaa 
      cgcaacgca aagctcatg cgaaacgtg ttcgtgaga 
      cgaggcgtt cgactcaag gtgtaacat tggaggaga 
      taaaatcgg ctgaacaat tcgttaaaa agaagaatc 
      aaccgaatt gcaataaca acaataacg agcaatctg 
      gagctaacg gggattccc ctaacggag tgagtgtga 
      ccgctacaa taaccactg cggcaccta ctgttataa 
      cacgtgata ctgaagaac ccagtatta ctcatgagc 
      gtaggccgt cgtggaggc tgaaaattg acagtagta 
      gtaagttta aactggaag atgcaacga aatcctctg 
      aaccaccaa gcactccgt cctttgtcc gtcgcaccg 
      tcacacaaa ataccacca cgaatccta gaccgtcat 
      cggcaccgg tcgaaacgg ccgaaacta cggtcacgt 
      taccattga gaagaccaa aaactcaag atgaaaaag 
      aacaagaca aagaaaatg acgtacgca acgctcaag 
      ccactctcg ccacacagg cggccatcc aacgaagaa 
      ggaggccca agaggaggt ccacagggg tcgtctcgt 
      ttgaaaata tggatgata tggatcaag ataaggatc 
      cgtcaggag gtggtgatt atgaagata gtacgaaat 
      taaatcacg aagagagtg ataggtcga gcagatctc 
      gtttaggaa gcacttcgg atattagga acgaaaacg 
      gcgaaattg actataaaa aattgtatg aacaagcac 
      ttgtcgaaa acaatgaac tcaaggaca aactgaaaa 
      agtcagatc aagaattga gggatacaa aacagacac 
      tggagaagc tcaaagttg tgacgagta aaaattcgt 
      tatcggaat tagagaaac gcgaacgga gggcaatgg 
      aacgaaagt tgagtgaga tggaggagg aattaaagc 
      agttggata ttatgaagg tagaaaatc aaaagctaa 
      aggacgaga atggagcgc ttattagag tgattagta 
      agttatcga aatagcata agccttaga agttttaat 
      tttcctatt gcattttac tctgattac tagaacttc 
      gtagtccga agtcgtttc cttcctttt tttgttgta 
      atcaagtat tattgttat tttttattt attgaaaat 
      tgtacgaaa cgaatgacc acattcaag taatgtctg 
      cttcaaatc ggtgaattt gaggcaaat tttacattt 
      tgacataat aaggcattt gtgttggat tgcttgtag 
      tttgctggt tgtgtgata ttatttgct ttttattta 
      ttttgacat tgtaggaaa agacatgta ttaaacact 
      tcattttta cctactaaa cgaacatgt caatttaaa 
      gtatatttt ttgtaaaac atataaaca tgttgtatt 
      atataaatg tatttaaat gtactgtac tttgtgaat 
      aacttatta ttaacaatt gttgtgatt ttaatgtta 
      caaaaaata tataaatta tttgttttt tcttgatat 
      ttgttttat aatgcttgc atttgatca aatataagc 
      attataaaa gtttgtttt ccttaggta atgtacaaa 
      atcgatttt taacgaaaa gtttaaatt gttagaatc 
      tagtccaaa gatgaaaaa cttgcgaca aattttgtt 
      gagcacttt tcacttgtt agatgtttt taactttga 
      aattatgcc ataaggtgc taaatatca ctaagttca 
      aatcaaaac tttacatat gaattttgt ctgtgacca 
      taataataa aaattggac tgatgaatg ttttgtttc 
      attagcgaa attacattt gtacataca gtttgttaa 
      tttctagcg ttttaacgt tttatttca ttgaacttg 
      tgccttgct tctctttgt atttattat ataaatttt 
      tagttagtg tgtaaatta tttcatgta tttattcct 
      tgtttagag gctggtaga atatcatgt gaatagtga 
      ttttatagc tgattcttt aataaaatc ctgatacga 
      at
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