| General information | |||
| TC003207 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 219820..236397 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC003207 | ||
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_00531 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 2 (9 dpi)Days post injection
Fertility
mature egg deposition 4 number 2nd lateral oviduct: 5 number 1st lateral oviduct: blocked -
Number of animals showing the phenotype: 4
Analysis of larval stage 1 cuticle
number of eggs/embryos/larvae on slide: > 50
larva cuticle duplicated - Penetrance (percentage of animals/eggs on slide): 50% - 80%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
larva cuticle duplicated - Penetrance (percentage of animals/eggs on slide): 50% - 80%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
Days post injection
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
Days post injection
eclosion delayed (still pupa) - Number of animals showing the phenotype: 7 (16 dpi)
Days post injection
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 7 (19 dpi)
Days post injection
Pupal morphology
abdomen elongated -
Number of animals showing the phenotype: 7
(16 dpi)
abdomen gin-traps structure irregular - Number of animals showing the phenotype: 7 (16 dpi)
female genital lobes rounded - Number of animals showing the phenotype: 4 (16 dpi)
abdomen gin-traps number increased - Number of animals showing the phenotype: 7 (16 dpi)
Days post injection
abdomen gin-traps structure irregular - Number of animals showing the phenotype: 7 (16 dpi)
Days post injection
female genital lobes rounded - Number of animals showing the phenotype: 4 (16 dpi)
Days post injection
abdomen gin-traps number increased - Number of animals showing the phenotype: 7 (16 dpi)
Days post injection
- >TC003207 RA
MTIHSNERL FLRDFVSCV AGSSGGRLA QWLQPASRV DPSKCQVLY SREELRCGW PAIITVVTR NQYSDLVNV PNMKVEVKA IPIDKKELG ECDSGRKIP RVMQQPDPL SFGGFVPPP IHNSYEPTI RDKMCFHSI SVMKSYQNY SFEELRYAC PTVKRSSEN MLVRHNSDG TYSATWTPA SVGFYSIVV NIDGYDMEE AFKVEVKEP PQGMTPPSQ ITAKKSIYQ PSKLRKFVA QNSAGLRIR AHPSLQSEQ IGVVHVNGT IAFIDEIHN DDGVWLRLS AETIKQYCT TCVIEAWCL QYNQHLGKT LLLPVEEPK SILDQVISD TIMRKLPEI SDRHKIPGS STNYQVIKC GASGHNIRS RPSLKAPPV GMLVLGNRI GVIDYAVNP DGYWVLLDK TTAEKYCFN TDHEAWSLA IGPNNVLYL GNVTGTDPH VLSEPEFVK HQAKKGFDF SCSSAPKPP EPNFSFSGQ PIQNLVVTP KETLSTNPF VFGDSVKGE SPKLPKRER KDSKLSSLP KWFKGDDVN KIDDSSREH GCNGNGITP PGTPKRSQS PRAGHLSPK PMSRSSSPV PIPSRGGNI LHDSTSSSP QAFGSPRSI GMSPLVRRG SNQSDTSAL VSSLTRDLS QSPSQATQP RDLSPSPSG SSLHTSPPH TPNKKENTD SEQSSKKLT QTGTQTSPE NSSNPSIKG HFSIGSGSK DERMPQKVS RVDRATGKA RPKRAISPA SLQQLPTSS KVNYHGSEP AKHAMSPSV AESLRAVFA AFLWHEGIV HDAMACASF LKFHPTLPK QGALVVTRQ PEAPVDKRR QELSKEEKA RQRHSVEVS NAGNYLHIQ PSTLESLTR SAANASANR KRPNKYPVI INEESGEKY TEGGYGYHT ISVLPPALK SLVFLWEEL TTNCLQAIS HDMIVASPV HKKKAGKDK EKNATIEKD SKKSRKKKI SPRNYLEDI GLSSYLHSS SWETLCELC GEVFPHPVT YHMHQSHPG CGQHAGGKG YNSGGNYCL GWAGNCGDG GIPNSSWYL LCESCREKY VKTNRGGKQ QTKTKNLTR RKSVYVKPL SSPSTSPGL EPHVIMKNN AMFLLDLAS SADAVIARQ RRPSSSVMP SVSENNSPP DCGGPFSPV PPFQCLQAL GANLTPNEP EFYEKVLRQ QNLHEVGLG QRPLSEVSI SDNDSDSGK GLRFHRSVS MGTNGIPWS KTGLDGRII MLRKRNNSS SEIANEGGS SLLCNPSNA LQKLVPKID TSAIVKTET CDHTEGLVL LQRPVMQFL LQQHDLESL QLAMKQALR KAMCRVYAM QALNWLLKS VTQPICLHD LLWWFVTSL TPAEVETET DDDNRPAKK IDDQDMNVC EHPLSDITI AGDAVHPLP STFHALLQT IADLMVLLP MGSSLQQMA VRCWGLRFA PSDHSFLHR SQVFSNISK ILSRSEEME DCTISMHES HQSVLSQAT CSVECLKDL TPSIEIKAS SRQAMIGSL TDGSTETFW ESGDEDRNK TKSLTVVCA QNHHPTLIC VHIDNCRDL GNKVSSVTF YSGPNTDEM IKLRTVEVE SRILGGWVN CPLTETIDS VIRLELKGP DNSVRVRQI RVLGKIEGE SLKVGKQYN AATIQQRNC EAETLRVFR LITSQVFGK LLQGEEGQN LNDSGSISA SESLEPLEE SNDLREHMV GILFSRSKL THLQKQVIV HIVQAIRKE TERVREEWE TSLCSNNGL TNSGYSQDV TKNSDTYCF EMLSMVLAL SGSSVGRAY LSHQGGLLG DLLSLLHTG SARVQRQVT SLLRRILPE VTPDSFAKV MGITNLPPK DFSIVSATY RGSFDMHKP GILDVFLSV IAKALTVQV KIKSKNGAG GAPKEINTV TLATSIYPK DNLGKRWWL RGCVNRKLA EVIVHLIKD MACGKLSEM WANVTKGAI AENILALTQ LSEEQRVPT TCLRTPTLW LALASLCVL DKDHVDRLS SGQWSNAEG QPAPPRPTC TNHDDGETN AVIQCNTCG NLCADCDRF LHLHRKTRN HQRQVCKEE EEAIKVELH EGCGRTKLF WLLALADAR TLKALIEYR DGGARSRGA GMSGVCRFC GATGSSGLL AIGNVCADH ECQEYGRTA CPKLLNCGH MCGGILGEN VCLPCLHGC SNEPNLRQD ADDMCMICF TEALSCAPA IQLKCGHVF HLHCCKAVL MKRWAGPRI TFSFSLCPI CKEEIVHPT LDELLAPIR DLYKDVKRK ALMRLEYEG LHTVEVIVT PGARFYNDP ASYAMDRYA YYVCYKCNK AYYGGEARC DAEVGENYD PTELVCGGC SDVARAQMC PKHGTDFLE YKCRYCCSV AVFFCFGTT HFCNPCHDD FQRVTNLSK TELPSCPAG PKAKQLEGD ECPLHVKHP PTGEEFALG CGVCRNAHT F
- >TC003207 RA
atgaccatc cactcaaac gagagactt ttcctgcga gatttcgtg agctgtgtg gccggttca agtggcggc cgtttagcc caatggctt cagccagcc agcagagta gatccatca aaatgtcaa gttttgtat tctcgagaa gagttacgt tgtggatgg ccggctatt ataacagtt gttacgcga aaccaatac tctgattta gttaatgtt ccaaatatg aaagtggag gttaaagca attccgatt gataaaaaa gagttgggg gaatgcgat tctgggcga aaaataccc cgagtgatg caacaacca gaccctttg agttttggt ggttttgta ccaccgccg atacacaac tcttacgag cccacaatt cgggataaa atgtgtttc cattcgatt agtgtcatg aagtcgtat cagaactac tcgtttgag gaattacga tacgcctgt cctacggtg aaaagatcc agcgagaat atgttagtg agacacaac agtgatggg acttatagt gccacttgg acacccgca tccgtcggt ttctactcg attgttgtc aatatagac ggttacgat atggaggaa gcgttcaaa gttgaagtt aaggaaccg ccacaagga atgacacca cctagtcaa atcacggcg aaaaaatcc atctatcag ccgagcaaa ttgaggaaa tttgtggct caaaattca gctggctta aggataagg gcgcatccg tccttgcag agcgaacag attggggtc gtgcatgtc aatggcacg attgccttt atcgatgag attcacaac gacgacggc gtgtggttg cgtctctcc gccgaaacg ataaaacag tactgcacc acgtgcgta atcgaagca tggtgtttg caatacaac caacatttg ggcaaaaca cttttactg ccagttgaa gaacccaaa tcaatttta gaccaagtt ataagcgac acaatcatg cgaaaattg ccggaaatt agcgaccgg cacaaaatc cccggcagt tctaccaac tatcaagtg ataaaatgt ggtgcttcc ggccacaat atccgaagt cggcccagc ctgaaagca ccacctgtt ggaatgcta gtattgggt aatcgaatt ggtgttatc gactatgcg gtcaatcct gatggttat tgggtgctg ttggataaa accacagct gagaaatat tgttttaat acggaccat gaagcttgg tcattggca attggaccg aataatgtt ctatatttg ggaaatgtg acagggact gatcctcat gtcttgtca gagcctgaa tttgtaaaa catcaagca aaaaaaggg tttgatttt tcgtgtagc tcagcaccg aaacctccc gaaccgaac ttttcattc agtggtcaa ccaatccag aatttggtt gtaacacca aaggaaaca ctttcgacg aatccgttt gtttttggt gatagtgtt aaaggcgaa tcgcccaaa cttccaaaa cgggagagg aaagattcg aaattgagt agtctaccg aagtggttt aaaggcgac gatgttaat aaaatcgat gattcgtca agagaacat ggttgtaac ggtaatggt attacacca ccagggacc ccaaaacgg tctcaaagt cctcgtgct gggcattta tccccgaaa ccgatgagt cggagctca agtcctgtt ccaattcct tctcgtggt ggtaatatc ctccatgac agtaccagc agcagtcca caagctttc ggttcacct cgtagcatc ggaatgtca cctttagta cgtcgcggt tccaaccaa tccgatacc agcgctttg gtgagtagt ttaacacgt gacttatcg caatcacca agtcaagcc acacaacca cgtgactta tcaccaagt ccaagcggt agttcttta catacaagt ccaccacac acaccaaac aaaaaggaa aacactgac tcggaacaa agttccaaa aaattgaca caaaccggg acgcaaact tcacccgaa aacagctca aacccttcg attaaaggc cacttttca atcggaagt ggcagtaaa gatgaaaga atgccacaa aaagtgtca cgagttgat cgcgccact ggtaaagcg cgccctaaa agggccata agtccggct agtttacag caattacct acttcttcc aaagtgaat tatcatggt tctgagccg gcaaaacac gcaatgagt ccctcggtg gctgagtca ttgcgggcc gttttcgct gcgtttctc tggcatgaa ggaatcgtc catgatgcc atggcttgt gcctcattc ctgaagttt cacccaacg ttgccgaaa cagggtgct ttggttgtg acccgacaa ccagaagct ccagtcgat aaaagacga caggagttg tccaaagaa gaaaaagca agacaaagg cattcggtt gaggttagc aatgcgggg aattaccta catattcaa ccatcaacg ttggaatcg ttgacgagg tccgcagct aatgctagt gccaataga aaaaggccc aataaatat ccagtgatt ataaatgag gaaagtgga gagaagtac actgagggt ggttatggg taccatacg atttcagtg ttaccacct gctttgaaa tcgcttgtt tttctctgg gaggagctg acgacgaat tgtctgcaa gctatttcg catgatatg attgtggcg agtcctgtg cataaaaag aaagcagga aaggataag gagaagaat gcaactatt gagaaagat agcaagaag agtaggaag aaaaagatt tcaccgagg aattatttg gaggacatc ggtctttcg agctacttg cactcttca agttgggaa accctctgc gagctctgt ggcgaagtg ttcccccac cctgttacc taccacatg caccaatcc caccctggt tgtggtcaa cacgctgga ggtaaaggc tacaattct ggcggaaat tattgttta ggttgggcc ggtaattgt ggtgatggt ggtattccc aacagttcg tggtacctc ctttgcgaa agttgtcgc gaaaaatac gtcaaaaca aaccgtgga ggcaaacaa caaaccaaa accaaaaac ctaactcgc agaaaaagt gtttacgtt aaaccgtta tcatcaccg tcaacttca ccaggtctc gaaccacat gtgattatg aaaaataac gcaatgttt ttattggat ttggcgagt tctgctgat gctgttata gcccgtcag aggagacca tcgtctagt gttatgcca tcagtttct gagaataat tcacctccg gattgtggt ggacctttt agtccagtg ccaccgttt cagtgtttg caagctttg ggggcgaat ttaacgccg aatgaaccc gagttttat gaaaaggtt ttaaggcaa cagaatttg cacgaagtt ggcttgggt caaagaccg ttgtcggag gtttccatt tcggataat gattcagat agtggtaaa gggttacgg tttcatcgc tcggtttca atgggcact aatgggatt ccctggtcc aagactggt ttagacggg aggattatt atgttgagg aaacggaat aatagtagt agtgaaatt gcaaacgaa ggaggatca tctctcctt tgcaaccca tcaaacgcc ctccaaaaa ctagttcca aaaattgac acttcggca attgtaaaa accgaaacg tgtgatcat actgaaggt cttgttctg ttacaacgg ccggtaatg cagtttttg ttgcaacaa cacgatttg gagtcgctc cagttggct atgaagcaa gcacttagg aaggcaatg tgtcgagtt tatgcaatg caagcttta aattggctt ttaaaatcg gtgactcag ccgatatgt ttgcacgat ttattgtgg tggtttgtc acttcgcta acacccgcc gaagttgag acggaaacg gacgatgat aaccgtcca gcgaaaaaa attgacgat caagacatg aacgtgtgc gaacaccca ctttccgat atcacaata gcaggggat gctgtccat cctctccct agcacattt catgccctt ctccagacc attgccgac cttatggta cttttaccc atgggttca tcattgcaa cagatggca gttaggtgt tggggctta agatttgca cccagtgac cattcattc ctgcacaga tctcaagtt tttagtaat attagtaaa attctatca cgaagtgaa gaaatggaa gattgtacg atttcgatg cacgaaagc catcaaagt gttctcagt caagccact tgtagtgtt gagtgtctc aaagatttg acaccatcc attgaaatt aaggcttca agtagacag gcaatgatt gggagtttg actgatggt tcgactgaa acgttttgg gagtcggga gatgaagat aggaataaa accaaaagt ttaactgtt gtgtgtgca caaaaccat catccgact ttgatttgt gtgcacatt gataattgt cgcgatttg gggaacaag gtttcaagt gttacgttt tattccggt ccaaatacc gatgaaatg atcaagtta cgaacggtt gaagtcgag tctagaatt ttaggtggt tgggtcaat tgtcctctc actgaaacc atcgatagt gtgatacgt ttggagctc aaaggacct gataattcg gtccgtgtt agacaaatt cgagttttg ggtaaaatc gaaggtgag tcgttaaaa gtgggcaaa caatacaat gctgccacc atccagcaa cgaaactgc gaagctgaa acactcaga gtgtttcgt ttaatcaca tcgcaagtt ttcggaaag ttgcttcaa ggtgaagaa ggccaaaat ttgaatgac agtggttcg atttcggca agtgaaagt ctcgaaccg ttggaagaa agcaacgat ttgagagaa cacatggtt gggatttta ttcagtcga agtaaacta acacatttg caaaaacaa gtgattgtc catatcgtc caagcgatt cgtaaggag actgaacgt gtacgtgaa gaatgggaa acatcgctt tgttcaaat aacggactg acaaacagt ggttatagt caggatgtt acgaaaaat tccgatact tattgtttt gagatgttg tcaatggtt ttggcttta tcgggaagt tcagtcgga cgggcttat ttgtcgcat caaggaggc ctgctcggg gatttactc agtcttttg catacagga agtgcacga gttcaacga caggtcact tcacttttg cgtcgaatt cttcccgaa gtcacccca gattcgttt gccaaagtg atgggaatc accaattta ccaccgaaa gatttcagc atagtctct gcaacatat cgtggttcc ttcgatatg cacaaacct ggaattttg gacgtattt ctgagcgtc atagctaaa gcattaacc gttcaagtg aaaatcaag agtaaaaat ggagcgggt ggagccccc aaagaaatc aatactgtg actttagcc acctccatt taccctaaa gacaatttg ggcaaacgt tggtggtta cgtggttgt gttaaccga aaactggcc gaagttata gttcacttg atcaaagat atggcctgt gggaaatta tcggaaatg tgggccaat gtaacaaaa ggggctatc gcagagaat attttagct ttgactcag ttgagtgag gaacaaaga gtgccgact acatgtctc aggactcca acgttgtgg ttggcactt gcttcactt tgtgtcctt gataaggat catgttgac cggttatcg tcaggacag tggagtaat gcagaagga caaccagct ccacctagg ccaacttgt acgaatcat gatgatggg gaaacgaat gcagttatt cagtgcaac acttgtggg aatctttgc gctgattgc gatcgcttt ttgcacctt catcgcaaa acacgcaat caccagaga caagtatgt aaagaggag gaggaagca atcaaagtc gaactacac gaagggtgc ggtcgtaca aaactcttc tggttgttg gctttagcc gatgctcgg actcttaaa gctctgatt gaatatcgc gacggcggt gcccggagt cgtggtgcc ggaatgagt ggagtttgt cgcttttgt ggcgcgact ggaagttcc ggtcttttg gcgattgga aatgtgtgt gcagatcat gaatgtcaa gaatatgga cgtactgca tgtcccaaa ttactaaac tgtggacat atgtgtggg ggaatccta ggcgaaaat gtgtgttta ccctgtctt catggttgc tccaatgaa ccgaatttg agacaagat gctgacgat atgtgcatg atttgcttt acggaggcg ttgtcgtgc gctcctgcc attcagctg aaatgcggt cacgtgttc cacttacac tgctgtaag gcggtgctt atgaaacga tgggcgggg cctcggatc acgttctcg ttttcgctt tgtccaatc tgtaaagag gaaatagtt catccgact ttggacgaa cttttagcc cccattcgg gatttatat aaagatgtg aaacggaaa gctttgatg cgtttggaa tatgaaggc ttgcacacg gtcgaggtt attgtcaca cctggggca aggttttac aatgatcca gcctcctat gccatggat agatacgcc tattatgtt tgctacaag tgcaataaa gcgtattat gggggcgaa gctcggtgt gatgctgaa gtgggtgaa aattatgac cccactgag ttagtatgt gggggctgt tcggatgtg gctagggcg cagatgtgc cccaaacac ggcactgat tttctcgag tacaagtgt cgttattgt tgttccgtt gcagtgttt ttctgtttt gggacaaca catttttgt aatccatgt cacgatgat tttcaaagg gtgacgaat ttgtcaaag actgaatta ccaagttgt ccagcgggt ccgaaagct aaacaattg gagggcgac gagtgtcca cttcatgtt aagcatcca ccaacgggg gaggagttt gctttgggg tgtggcgtc tgcagaaac gctcacacg ttttaa
- >TC003207 RA
tttttaacc ggaaatctg gcgggttta tttaataaa gccaccccc ataaaaggg attagtagt tagtttggt tccacttgc ctgaactcc actcaggga gcaattaac actcttcca cttggccac ctttacggt gcaattgca gccctttag gtcacatat cgtgtctcc tctcccgag ctttatccc aaaaaaata cacccacag aagcacaaa gaaggaccg ccacgatcg gacacccat ttcgtacac tcattcttg cactggttg tacatcggt tacgtttgg cacaaacac tgcaaacga catgatttg acctgcatg agtctgttt tggtgcttt tttgcgata gggaaacta tcacttggg actgttctg aacgactgg cgcttttga attgctttg ctttgctct tggctggtg tttcatgtg gtgtctttg tttggggcg ctgttgtct ctcttggga gtgtggagg atgaccatc cactcaaac gagagactt ttcctgcga gatttcgtg agctgtgtg gccggttca agtggcggc cgtttagcc caatggctt cagccagcc agcagagta gatccatca aaatgtcaa gttttgtat tctcgagaa gagttacgt tgtggatgg ccggctatt ataacagtt gttacgcga aaccaatac tctgattta gttaatgtt ccaaatatg aaagtggag gttaaagca attccgatt gataaaaaa gagttgggg gaatgcgat tctgggcga aaaataccc cgagtgatg caacaacca gaccctttg agttttggt ggttttgta ccaccgccg atacacaac tcttacgag cccacaatt cgggataaa atgtgtttc cattcgatt agtgtcatg aagtcgtat cagaactac tcgtttgag gaattacga tacgcctgt cctacggtg aaaagatcc agcgagaat atgttagtg agacacaac agtgatggg acttatagt gccacttgg acacccgca tccgtcggt ttctactcg attgttgtc aatatagac ggttacgat atggaggaa gcgttcaaa gttgaagtt aaggaaccg ccacaagga atgacacca cctagtcaa atcacggcg aaaaaatcc atctatcag ccgagcaaa ttgaggaaa tttgtggct caaaattca gctggctta aggataagg gcgcatccg tccttgcag agcgaacag attggggtc gtgcatgtc aatggcacg attgccttt atcgatgag attcacaac gacgacggc gtgtggttg cgtctctcc gccgaaacg ataaaacag tactgcacc acgtgcgta atcgaagca tggtgtttg caatacaac caacatttg ggcaaaaca cttttactg ccagttgaa gaacccaaa tcaatttta gaccaagtt ataagcgac acaatcatg cgaaaattg ccggaaatt agcgaccgg cacaaaatc cccggcagt tctaccaac tatcaagtg ataaaatgt ggtgcttcc ggccacaat atccgaagt cggcccagc ctgaaagca ccacctgtt ggaatgcta gtattgggt aatcgaatt ggtgttatc gactatgcg gtcaatcct gatggttat tgggtgctg ttggataaa accacagct gagaaatat tgttttaat acggaccat gaagcttgg tcattggca attggaccg aataatgtt ctatatttg ggaaatgtg acagggact gatcctcat gtcttgtca gagcctgaa tttgtaaaa catcaagca aaaaaaggg tttgatttt tcgtgtagc tcagcaccg aaacctccc gaaccgaac ttttcattc agtggtcaa ccaatccag aatttggtt gtaacacca aaggaaaca ctttcgacg aatccgttt gtttttggt gatagtgtt aaaggcgaa tcgcccaaa cttccaaaa cgggagagg aaagattcg aaattgagt agtctaccg aagtggttt aaaggcgac gatgttaat aaaatcgat gattcgtca agagaacat ggttgtaac ggtaatggt attacacca ccagggacc ccaaaacgg tctcaaagt cctcgtgct gggcattta tccccgaaa ccgatgagt cggagctca agtcctgtt ccaattcct tctcgtggt ggtaatatc ctccatgac agtaccagc agcagtcca caagctttc ggttcacct cgtagcatc ggaatgtca cctttagta cgtcgcggt tccaaccaa tccgatacc agcgctttg gtgagtagt ttaacacgt gacttatcg caatcacca agtcaagcc acacaacca cgtgactta tcaccaagt ccaagcggt agttcttta catacaagt ccaccacac acaccaaac aaaaaggaa aacactgac tcggaacaa agttccaaa aaattgaca caaaccggg acgcaaact tcacccgaa aacagctca aacccttcg attaaaggc cacttttca atcggaagt ggcagtaaa gatgaaaga atgccacaa aaagtgtca cgagttgat cgcgccact ggtaaagcg cgccctaaa agggccata agtccggct agtttacag caattacct acttcttcc aaagtgaat tatcatggt tctgagccg gcaaaacac gcaatgagt ccctcggtg gctgagtca ttgcgggcc gttttcgct gcgtttctc tggcatgaa ggaatcgtc catgatgcc atggcttgt gcctcattc ctgaagttt cacccaacg ttgccgaaa cagggtgct ttggttgtg acccgacaa ccagaagct ccagtcgat aaaagacga caggagttg tccaaagaa gaaaaagca agacaaagg cattcggtt gaggttagc aatgcgggg aattaccta catattcaa ccatcaacg ttggaatcg ttgacgagg tccgcagct aatgctagt gccaataga aaaaggccc aataaatat ccagtgatt ataaatgag gaaagtgga gagaagtac actgagggt ggttatggg taccatacg atttcagtg ttaccacct gctttgaaa tcgcttgtt tttctctgg gaggagctg acgacgaat tgtctgcaa gctatttcg catgatatg attgtggcg agtcctgtg cataaaaag aaagcagga aaggataag gagaagaat gcaactatt gagaaagat agcaagaag agtaggaag aaaaagatt tcaccgagg aattatttg gaggacatc ggtctttcg agctacttg cactcttca agttgggaa accctctgc gagctctgt ggcgaagtg ttcccccac cctgttacc taccacatg caccaatcc caccctggt tgtggtcaa cacgctgga ggtaaaggc tacaattct ggcggaaat tattgttta ggttgggcc ggtaattgt ggtgatggt ggtattccc aacagttcg tggtacctc ctttgcgaa agttgtcgc gaaaaatac gtcaaaaca aaccgtgga ggcaaacaa caaaccaaa accaaaaac ctaactcgc agaaaaagt gtttacgtt aaaccgtta tcatcaccg tcaacttca ccaggtctc gaaccacat gtgattatg aaaaataac gcaatgttt ttattggat ttggcgagt tctgctgat gctgttata gcccgtcag aggagacca tcgtctagt gttatgcca tcagtttct gagaataat tcacctccg gattgtggt ggacctttt agtccagtg ccaccgttt cagtgtttg caagctttg ggggcgaat ttaacgccg aatgaaccc gagttttat gaaaaggtt ttaaggcaa cagaatttg cacgaagtt ggcttgggt caaagaccg ttgtcggag gtttccatt tcggataat gattcagat agtggtaaa gggttacgg tttcatcgc tcggtttca atgggcact aatgggatt ccctggtcc aagactggt ttagacggg aggattatt atgttgagg aaacggaat aatagtagt agtgaaatt gcaaacgaa ggaggatca tctctcctt tgcaaccca tcaaacgcc ctccaaaaa ctagttcca aaaattgac acttcggca attgtaaaa accgaaacg tgtgatcat actgaaggt cttgttctg ttacaacgg ccggtaatg cagtttttg ttgcaacaa cacgatttg gagtcgctc cagttggct atgaagcaa gcacttagg aaggcaatg tgtcgagtt tatgcaatg caagcttta aattggctt ttaaaatcg gtgactcag ccgatatgt ttgcacgat ttattgtgg tggtttgtc acttcgcta acacccgcc gaagttgag acggaaacg gacgatgat aaccgtcca gcgaaaaaa attgacgat caagacatg aacgtgtgc gaacaccca ctttccgat atcacaata gcaggggat gctgtccat cctctccct agcacattt catgccctt ctccagacc attgccgac cttatggta cttttaccc atgggttca tcattgcaa cagatggca gttaggtgt tggggctta agatttgca cccagtgac cattcattc ctgcacaga tctcaagtt tttagtaat attagtaaa attctatca cgaagtgaa gaaatggaa gattgtacg atttcgatg cacgaaagc catcaaagt gttctcagt caagccact tgtagtgtt gagtgtctc aaagatttg acaccatcc attgaaatt aaggcttca agtagacag gcaatgatt gggagtttg actgatggt tcgactgaa acgttttgg gagtcggga gatgaagat aggaataaa accaaaagt ttaactgtt gtgtgtgca caaaaccat catccgact ttgatttgt gtgcacatt gataattgt cgcgatttg gggaacaag gtttcaagt gttacgttt tattccggt ccaaatacc gatgaaatg atcaagtta cgaacggtt gaagtcgag tctagaatt ttaggtggt tgggtcaat tgtcctctc actgaaacc atcgatagt gtgatacgt ttggagctc aaaggacct gataattcg gtccgtgtt agacaaatt cgagttttg ggtaaaatc gaaggtgag tcgttaaaa gtgggcaaa caatacaat gctgccacc atccagcaa cgaaactgc gaagctgaa acactcaga gtgtttcgt ttaatcaca tcgcaagtt ttcggaaag ttgcttcaa ggtgaagaa ggccaaaat ttgaatgac agtggttcg atttcggca agtgaaagt ctcgaaccg ttggaagaa agcaacgat ttgagagaa cacatggtt gggatttta ttcagtcga agtaaacta acacatttg caaaaacaa gtgattgtc catatcgtc caagcgatt cgtaaggag actgaacgt gtacgtgaa gaatgggaa acatcgctt tgttcaaat aacggactg acaaacagt ggttatagt caggatgtt acgaaaaat tccgatact tattgtttt gagatgttg tcaatggtt ttggcttta tcgggaagt tcagtcgga cgggcttat ttgtcgcat caaggaggc ctgctcggg gatttactc agtcttttg catacagga agtgcacga gttcaacga caggtcact tcacttttg cgtcgaatt cttcccgaa gtcacccca gattcgttt gccaaagtg atgggaatc accaattta ccaccgaaa gatttcagc atagtctct gcaacatat cgtggttcc ttcgatatg cacaaacct ggaattttg gacgtattt ctgagcgtc atagctaaa gcattaacc gttcaagtg aaaatcaag agtaaaaat ggagcgggt ggagccccc aaagaaatc aatactgtg actttagcc acctccatt taccctaaa gacaatttg ggcaaacgt tggtggtta cgtggttgt gttaaccga aaactggcc gaagttata gttcacttg atcaaagat atggcctgt gggaaatta tcggaaatg tgggccaat gtaacaaaa ggggctatc gcagagaat attttagct ttgactcag ttgagtgag gaacaaaga gtgccgact acatgtctc aggactcca acgttgtgg ttggcactt gcttcactt tgtgtcctt gataaggat catgttgac cggttatcg tcaggacag tggagtaat gcagaagga caaccagct ccacctagg ccaacttgt acgaatcat gatgatggg gaaacgaat gcagttatt cagtgcaac acttgtggg aatctttgc gctgattgc gatcgcttt ttgcacctt catcgcaaa acacgcaat caccagaga caagtatgt aaagaggag gaggaagca atcaaagtc gaactacac gaagggtgc ggtcgtaca aaactcttc tggttgttg gctttagcc gatgctcgg actcttaaa gctctgatt gaatatcgc gacggcggt gcccggagt cgtggtgcc ggaatgagt ggagtttgt cgcttttgt ggcgcgact ggaagttcc ggtcttttg gcgattgga aatgtgtgt gcagatcat gaatgtcaa gaatatgga cgtactgca tgtcccaaa ttactaaac tgtggacat atgtgtggg ggaatccta ggcgaaaat gtgtgttta ccctgtctt catggttgc tccaatgaa ccgaatttg agacaagat gctgacgat atgtgcatg atttgcttt acggaggcg ttgtcgtgc gctcctgcc attcagctg aaatgcggt cacgtgttc cacttacac tgctgtaag gcggtgctt atgaaacga tgggcgggg cctcggatc acgttctcg ttttcgctt tgtccaatc tgtaaagag gaaatagtt catccgact ttggacgaa cttttagcc cccattcgg gatttatat aaagatgtg aaacggaaa gctttgatg cgtttggaa tatgaaggc ttgcacacg gtcgaggtt attgtcaca cctggggca aggttttac aatgatcca gcctcctat gccatggat agatacgcc tattatgtt tgctacaag tgcaataaa gcgtattat gggggcgaa gctcggtgt gatgctgaa gtgggtgaa aattatgac cccactgag ttagtatgt gggggctgt tcggatgtg gctagggcg cagatgtgc cccaaacac ggcactgat tttctcgag tacaagtgt cgttattgt tgttccgtt gcagtgttt ttctgtttt gggacaaca catttttgt aatccatgt cacgatgat tttcaaagg gtgacgaat ttgtcaaag actgaatta ccaagttgt ccagcgggt ccgaaagct aaacaattg gagggcgac gagtgtcca cttcatgtt aagcatcca ccaacgggg gaggagttt gctttgggg tgtggcgtc tgcagaaac gctcacacg ttttaaaat gtacttatt caagctgga agtgattat tttgcaaag tattccgtt attatgacg ttccatttt tttattatg tgtaaagtt tgtttgagg gtcagcctg atcgttgtc tatttgaaa caaacgcaa ttttgactt attttattt atttaatat attcagaaa tgcattgta cctcgttta ataaaattg atgtaaaaa t
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