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Gene information
General information
TC003204 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid13: 294117..295617
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003204
    iBeetle screen iB_00530
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003204 RA
      MMFTKIITR NFLKDCDRV LVGLRPFSL SSNRNCDEK 
      KTQESPLKK LLDESATFE DLHPQTQEQ QWATLPYPQ 
      GTKIRKQGV SFIEKPKYD PRESSIILF PGQGSQFVG 
      MCNDLLKYP MARDLFELA NYILKYDLL KLCLEGPKD 
      KLDQTKYSQ PAIMVSSLA AIERLKEER PRAIESCVG 
      TAGFSLGEI TALVFAGAI GFERGLRLV QIRGEAMQL 
      ASEAYKGGM VTVMYGPDT KLNQALVKA KEWATDKGD 
      LMPECQIAN YLYPHCKVI SGSESALDY LEKNAKELN 
      LRKVKRLAV SGAFHSNLM LPAVEPFRK ALNKSEVSN 
      PVISVYSNV DGKKYKDAA QIKKQLPKQ IVKPVKWEQ 
      LLHVVYERS QGDYFPRTF ECGPGTSLR AILKQVNAK 
      AWTTCFNIQ V
    CDS sequence
    • >TC003204 RA
      atgatgttt actaaaatt attacacga aattttctg 
      aaagactgc gatcgtgtt ttggtaggg cttcgcccc 
      ttctccctg tcctcaaat cgtaactgt gatgagaaa 
      aaaacgcaa gaatctccc ctaaagaaa ctcctcgac 
      gaatcagca acctttgaa gacttgcac ccccagaca 
      caagaacaa caatgggct actttgcca taccctcaa 
      ggcaccaaa attcgcaaa cagggtgtc tcatttatc 
      gaaaaacct aaatatgat ccacgcgag tcctcgata 
      attttattt cccggtcag gggagccaa tttgtcggt 
      atgtgcaat gacttgctc aagtacccc atggctagg 
      gatttgttt gagttagcg aattatatt ttaaagtac 
      gatttgtta aaattgtgt cttgagggt cccaaagac 
      aaattggac caaacaaaa tatagtcaa ccggcaata 
      atggtcagt tctttagcg gcaattgaa cgactgaaa 
      gaggagaga cctagggct attgagagt tgtgtgggg 
      acagcaggg ttcagtttg ggggaaatt acagcgtta 
      gtgtttgcg ggggcgatt ggttttgag cgagggtta 
      cgattggtg caaattcgg ggggaagca atgcaattg 
      gctagtgag gcgtacaag ggggggatg gtcacagta 
      atgtacggt cctgacacg aagttaaac caggcacta 
      gtcaaagcc aaggaatgg gcaactgat aaaggcgac 
      ttaatgccc gaatgtcaa attgccaat tatttgtac 
      ccccattgt aaagtcatc tcggggagc gaatctgcg 
      cttgattat ttggaaaaa aatgccaaa gaattaaat 
      ttacgtaaa gttaaaaga cttgcggtt agtggagct 
      ttccattcc aatttaatg ttaccagcg gttgaaccg 
      tttcgaaag gcattaaat aaatcagaa gtgagcaat 
      cctgtaatt agtgtttat tcaaatgtt gacgggaaa 
      aagtacaaa gacgcagcg cagatcaaa aaacaattg 
      ccgaaacag attgttaaa cctgtaaag tgggaacaa 
      cttcttcat gttgtttat gagaggagt caaggggat 
      tatttccca aggacgttt gagtgtggc ccaggcacg 
      tccctaagg gctatcctt aaacaagtg aatgccaaa 
      gcatggact acgtgtttt aatattcaa gtgtag
    mRNA sequence
    • >TC003204 RA
      gtcgcaact tgacattcg aggttaagt tgaatcatt 
      aaaatgtgt ttttatttg aattagttt tgtgtgttt 
      tgcttgatt aacagttta attatacca tgatgttta 
      ctaaaatta ttacacgaa attttctga aagactgcg 
      atcgtgttt tggtagggc ttcgcccct tctccctgt 
      cctcaaatc gtaactgtg atgagaaaa aaacgcaag 
      aatctcccc taaagaaac tcctcgacg aatcagcaa 
      cctttgaag acttgcacc cccagacac aagaacaac 
      aatgggcta ctttgccat accctcaag gcaccaaaa 
      ttcgcaaac agggtgtct catttatcg aaaaaccta 
      aatatgatc cacgcgagt cctcgataa ttttatttc 
      ccggtcagg ggagccaat ttgtcggta tgtgcaatg 
      acttgctca agtacccca tggctaggg atttgtttg 
      agttagcga attatattt taaagtacg atttgttaa 
      aattgtgtc ttgagggtc ccaaagaca aattggacc 
      aaacaaaat atagtcaac cggcaataa tggtcagtt 
      ctttagcgg caattgaac gactgaaag aggagagac 
      ctagggcta ttgagagtt gtgtgggga cagcagggt 
      tcagtttgg gggaaatta cagcgttag tgtttgcgg 
      gggcgattg gttttgagc gagggttac gattggtgc 
      aaattcggg gggaagcaa tgcaattgg ctagtgagg 
      cgtacaagg gggggatgg tcacagtaa tgtacggtc 
      ctgacacga agttaaacc aggcactag tcaaagcca 
      aggaatggg caactgata aaggcgact taatgcccg 
      aatgtcaaa ttgccaatt atttgtacc cccattgta 
      aagtcatct cggggagcg aatctgcgc ttgattatt 
      tggaaaaaa atgccaaag aattaaatt tacgtaaag 
      ttaaaagac ttgcggtta gtggagctt tccattcca 
      atttaatgt taccagcgg ttgaaccgt ttcgaaagg 
      cattaaata aatcagaag tgagcaatc ctgtaatta 
      gtgtttatt caaatgttg acgggaaaa agtacaaag 
      acgcagcgc agatcaaaa aacaattgc cgaaacaga 
      ttgttaaac ctgtaaagt gggaacaac ttcttcatg 
      ttgtttatg agaggagtc aaggggatt atttcccaa 
      ggacgtttg agtgtggcc caggcacgt ccctaaggg 
      ctatcctta aacaagtga atgccaaag catggacta 
      cgtgtttta atattcaag tgtagagtt tattttgaa 
      taaaatttt tgaaacaac