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Gene information
General information
TC003201 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 305743..307947
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003201
    iBeetle screen iB_00529

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003201 RA
      MKFYVESVS KCAQRLGTL SEIDAKPSV SIKTPAVML 
      HTQGGQIPH ITHEVFKLV SRDPQILQI PLAGMHTFK 
      EAMQYYEGT ISNFIGSKD SLTCLTLQD PCNITKQGH 
      NSTLKVPIW TKNGKVYYD AKSYMDVVE SFKPDIYFL 
      LSDGDTNIS SSEKRISKS LSNTVSFYK ECLERHRKS 
      QTLANSFVI APVVGGYKQ LAREKFLKS ILADVKDVG 
      GFLIDGLHN NGPETEFLE FAEIEPVVK NILNKLPTD 
      KLRVIQGCW NPVNVINLV EMGIDLFDS SYCHILTER 
      SAAMTFAIE PTDQTDTYE INLRDAKYL DDFQPILTG 
      CNCTSCTKY SRAYIHHLL SVHELLGPI LVMIHNVYH 
      YLRFFEKIR KCVKSNTLQ ALQVSITEQ FQNHQKTK
    CDS sequence
    • >TC003201 RA
      atgaaattt tacgtggaa tctgtctca aagtgtgcc 
      caacgtttg gggactctt tcagagatc gatgccaag 
      ccttctgtc tcgatcaag actcctgca gtaatgctc 
      cacacacag ggcggtcaa atccctcac attacacat 
      gaggtgttt aaactggtc agtagggac ccccagatc 
      ttgcaaatc cctttagct gggatgcac acgtttaaa 
      gaggcgatg cagtattac gaagggacc atttccaat 
      ttcattggt agtaaagac agtctgact tgtctcact 
      cttcaagac ccttgcaat atcacaaaa caggggcac 
      aattccacg ttaaaagtg cccatttgg acgaaaaac 
      ggcaaggtt tattacgac gctaaaagc tacatggac 
      gtggtggag tctttcaag cctgacatt tatttcttg 
      ctctctgat ggagacacc aatatcagc agttcggaa 
      aaacgcatt tcaaaatca ctcagtaac acagtcagt 
      ttctacaag gagtgtttg gagagacac cgaaaatcg 
      caaacttta gccaattcg tttgtgatt gctcctgta 
      gtggggggt tataaacaa ttagctcgg gaaaagttt 
      ctcaaatcg attctagca gatgtcaaa gatgtaggt 
      gggttttta atagatgga cttcataat aatggtccc 
      gagaccgag tttttggaa tttgctgaa attgagccc 
      gtggtgaaa aatattttg aataaatta cccactgat 
      aaactacgg gtcattcaa ggctgttgg aatcccgta 
      aatgttatt aatttggtc gaaatgggg attgatttg 
      tttgactcc tcatattgt catattctc actgaaaga 
      tcagcagca atgactttt gcgatagaa ccaacagat 
      cagacagac acgtatgaa ataaatcta agagacgca 
      aaatatttg gacgatttt cagccaatt ctaactggt 
      tgcaattgc acgtcttgc acaaaatat tcaagggca 
      tacatacac cacttgcta tcagtacat gagctcctg 
      gggccaatc ctcgtgatg atacacaat gtgtaccac 
      tatttgcga tttttcgaa aaaatccgc aagtgcgtg 
      aaatcaaac accttacaa gcactccaa gtgagcata 
      acggaacaa ttccaaaac caccaaaaa accaagtga
    mRNA sequence
    • >TC003201 RA
      aaaaaatgc caaaaaaac tacaaaaca accacaatt 
      aaataatga aaaaactgg caaagtttt ggtatatta 
      atgaatgaa atcatgtga agttcgttt ttaaaacgt 
      aaatgagtg tgtttttgc gccctgtca tctattttt 
      tcccatttg tgaggcatg caaaagtaa ggttaggcc 
      ggcatgtgt ccacttttt tcggatttt tgaatgtca 
      tgtttgctt tatttgtca aaacctaat tctggaagt 
      gattttagt tgtatagac tgctttttc acacttttc 
      cgagtgcat ttagtgtaa attaacgat gaaatttta 
      cgtggaatc tgtctcaaa gtgtgccca acgtttggg 
      gactctttc agagatcga tgccaagcc ttctgtctc 
      gatcaagac tcctgcagt aatgctcca cacacaggg 
      cggtcaaat ccctcacat tacacatga ggtgtttaa 
      actggtcag tagggaccc ccagatctt gcaaatccc 
      tttagctgg gatgcacac gtttaaaga ggcgatgca 
      gtattacga agggaccat ttccaattt cattggtag 
      taaagacag tctgacttg tctcactct tcaagaccc 
      ttgcaatat cacaaaaca ggggcacaa ttccacgtt 
      aaaagtgcc catttggac gaaaaacgg caaggttta 
      ttacgacgc taaaagcta catggacgt ggtggagtc 
      tttcaagcc tgacattta tttcttgct ctctgatgg 
      agacaccaa tatcagcag ttcggaaaa acgcatttc 
      aaaatcact cagtaacac agtcagttt ctacaagga 
      gtgtttgga gagacaccg aaaatcgca aactttagc 
      caattcgtt tgtgattgc tcctgtagt ggggggtta 
      taaacaatt agctcggga aaagtttct caaatcgat 
      tctagcaga tgtcaaaga tgtaggtgg gtttttaat 
      agatggact tcataataa tggtcccga gaccgagtt 
      tttggaatt tgctgaaat tgagcccgt ggtgaaaaa 
      tattttgaa taaattacc cactgataa actacgggt 
      cattcaagg ctgttggaa tcccgtaaa tgttattaa 
      tttggtcga aatggggat tgatttgtt tgactcctc 
      atattgtca tattctcac tgaaagatc agcagcaat 
      gacttttgc gatagaacc aacagatca gacagacac 
      gtatgaaat aaatctaag agacgcaaa atatttgga 
      cgattttca gccaattct aactggttg caattgcac 
      gtcttgcac aaaatattc aagggcata catacacca 
      cttgctatc agtacatga gctcctggg gccaatcct 
      cgtgatgat acacaatgt gtaccacta tttgcgatt 
      tttcgaaaa aatccgcaa gtgcgtgaa atcaaacac 
      cttacaagc actccaagt gagcataac ggaacaatt 
      ccaaaacca ccaaaaaac caagtgacg ggacaccaa 
      attgtacaa aattttcgt gttgtacga ctgtttttc 
      tattatttt taaataaat tgtccgttt gata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY