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Gene information
General information
TC003159 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 1019531..1030488
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003159
    iBeetle screen iB_00525

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003159 RA
      MLALPRMGS FSSAPKIQN DDNQDNDIQ IPINFGRFE 
      LLQYCQTQP QVQPMVKRA FKKAGQTND DTYGFKCRK 
      LNAVTKGCS DYVSSPYSL RVFQWNILS QALGQMNDH 
      FVKCPDEAL EWNSRKFRI IEEIVEYCP DIICLQEVD 
      HFNFLKYIL GTQGYTGVF YPKPDSPCV YISGNNGPD 
      GCAIFYRTN KFDVINIES RILEIWRVQ SNQVALLAN 
      LRIKETGQE VCVTTTHLK ARQGAFLST LRNEQGKDL 
      LQFVSQHCG PRPVVICGD FNAEPIEPI YSTILSDEY 
      LNLGSAYAD CDSSSANSA AREPPYTTW KIRDEGEVC 
      HTIDYIFYK KGCLEVEAV LELPTGEEI GEDRVPSFS 
      YPSDHFSLV CDFKIGQKA TL
    • >TC003159 RB
      MLALPRANV PVGFKMLEF DIGEEKILK NVRLQKVIL 
      QRMGSFSSA PKIQNDDNQ DNDIQIPIN FGRFELLQY 
      CQTQPQVQP MVKRAFKKA GQTNDDTYG FKCRKLNAV 
      TKGCSDYVS SPYSLRVFQ WNILSQALG QMNDHFVKC 
      PDEALEWNS RKFRIIEEI VEYCPDIIC LQEVDHFNF 
      LKYILGTQG YTGVFYPKP DSPCVYISG NNGPDGCAI 
      FYRTNKFDV INIESRILE IWRVQSNQV ALLANLRIK 
      ETGQEVCVT TTHLKARQG AFLSTLRNE QGKDLLQFV 
      SQHCGPRPV VICGDFNAE PIEPIYSTI LSDEYLNLG 
      SAYADCDSS SANSAAREP PYTTWKIRD EGEVCHTID 
      YIFYKKGCL EVEAVLELP TGEEIGEDR VPSFSYPSD 
      HFSLVCDFK IGQKATL
    • >TC003159 RC
      MSEIWQSQD GTINIRESL ALAETLSKI SLMTSPAVK 
      KRLMARSLE LKFGHLDED SGTFIPPKQ FLQYLVRMG 
      SFSSAPKIQ NDDNQDNDI QIPINFGRF ELLQYCQTQ 
      PQVQPMVKR AFKKAGQTN DDTYGFKCR KLNAVTKGC 
      SDYVSSPYS LRVFQWNIL SQALGQMND HFVKCPDEA 
      LEWNSRKFR IIEEIVEYC PDIICLQEV DHFNFLKYI 
      LGTQGYTGV FYPKPDSPC VYISGNNGP DGCAIFYRT 
      NKFDVINIE SRILEIWRV QSNQVALLA NLRIKETGQ 
      EVCVTTTHL KARQGAFLS TLRNEQGKD LLQFVSQHC 
      GPRPVVICG DFNAEPIEP IYSTILSDE YLNLGSAYA 
      DCDSSSANS AAREPPYTT WKIRDEGEV CHTIDYIFY 
      KKGCLEVEA VLELPTGEE IGEDRVPSF SYPSDHFSL 
      VCDFKIGQK ATL
    CDS sequence
    • >TC003159 RA
      atgctggcc ttaccaagg atgggcagc ttcagctca 
      gcgccgaaa atccaaaat gacgataac caagacaac 
      gatattcaa ataccgatc aactttggc cggtttgaa 
      ctgctccag tattgccag acccagccc caagtccag 
      cccatggtg aagagggcc ttcaagaaa gcgggacaa 
      accaacgac gatacttat ggtttcaag tgccggaag 
      ttgaacgcc gttaccaaa ggttgtagt gattacgtt 
      tctagtcct tactcgtta agagttttt cagtggaat 
      atactctca caagcgctg ggacaaatg aacgaccat 
      ttcgtgaaa tgtccagac gaagcgtta gaatggaac 
      tcgcggaaa ttccggatt attgaggaa attgtcgag 
      tattgtccc gatattatt tgtctacag gaagtggat 
      catttcaat tttctgaag tacatcctc ggcactcaa 
      ggttacacg ggggtattt tacccaaaa ccggactca 
      ccttgtgtt tacatcagt ggtaataat ggtcccgat 
      gggtgtgca attttttat cgaaccaac aaattcgat 
      gtaattaac attgaatcc cgtattttg gaaatctgg 
      agagtacaa agcaatcaa gtggccctt ttggcgaac 
      ctgcgcatc aaagagacc ggccaggag gtctgtgtg 
      accaccacg cacttgaag gcccgccaa ggcgccttc 
      ctgtccact ttgcgcaac gaacaaggc aaggatttg 
      ctccagttt gtgtcgcaa cattgcggc ccccgacct 
      gttgtcatc tgcggcgat tttaatgca gaacctatt 
      gagcccatc tacagtacc atcttatcg gacgaatat 
      ctaaatctt ggtagtgct tacgctgac tgtgatagt 
      tcgagcgcg aattcagcg gcgcgagaa cccccttac 
      accacgtgg aagataagg gacgagggc gaagtctgc 
      cacaccata gactatatt ttttacaag aagggctgt 
      ttagaggtt gaagctgtg ctggagttg cccaccggg 
      gaggaaatt ggagaggat agggttccc tcgttttcg 
      tatccttcg gatcatttt tcgctcgtt tgcgatttt 
      aagattggt caaaaggcg acgctttga
    • >TC003159 RB
      atgctggcc ttaccaagg gctaacgtg ccagttggc 
      ttcaaaatg ctagaattt gacatcggt gaggaaaag 
      atcttaaaa aatgttcga ttacagaaa gttattttg 
      caacggatg ggcagcttc agctcagcg ccgaaaatc 
      caaaatgac gataaccaa gacaacgat attcaaata 
      ccgatcaac tttggccgg tttgaactg ctccagtat 
      tgccagacc cagccccaa gtccagccc atggtgaag 
      agggccttc aagaaagcg ggacaaacc aacgacgat 
      acttatggt ttcaagtgc cggaagttg aacgccgtt 
      accaaaggt tgtagtgat tacgtttct agtccttac 
      tcgttaaga gtttttcag tggaatata ctctcacaa 
      gcgctggga caaatgaac gaccatttc gtgaaatgt 
      ccagacgaa gcgttagaa tggaactcg cggaaattc 
      cggattatt gaggaaatt gtcgagtat tgtcccgat 
      attatttgt ctacaggaa gtggatcat ttcaatttt 
      ctgaagtac atcctcggc actcaaggt tacacgggg 
      gtattttac ccaaaaccg gactcacct tgtgtttac 
      atcagtggt aataatggt cccgatggg tgtgcaatt 
      ttttatcga accaacaaa ttcgatgta attaacatt 
      gaatcccgt attttggaa atctggaga gtacaaagc 
      aatcaagtg gcccttttg gcgaacctg cgcatcaaa 
      gagaccggc caggaggtc tgtgtgacc accacgcac 
      ttgaaggcc cgccaaggc gccttcctg tccactttg 
      cgcaacgaa caaggcaag gatttgctc cagtttgtg 
      tcgcaacat tgcggcccc cgacctgtt gtcatctgc 
      ggcgatttt aatgcagaa cctattgag cccatctac 
      agtaccatc ttatcggac gaatatcta aatcttggt 
      agtgcttac gctgactgt gatagttcg agcgcgaat 
      tcagcggcg cgagaaccc ccttacacc acgtggaag 
      ataagggac gagggcgaa gtctgccac accatagac 
      tatattttt tacaagaag ggctgttta gaggttgaa 
      gctgtgctg gagttgccc accggggag gaaattgga 
      gaggatagg gttccctcg ttttcgtat ccttcggat 
      catttttcg ctcgtttgc gattttaag attggtcaa 
      aaggcgacg ctttga
    • >TC003159 RC
      atgagtgag atatggcaa tcgcaagac ggtaccatc 
      aacatccgg gagtctctg gcacttgca gagactcta 
      tccaaaatt agcctgatg acatctcca gcagtcaag 
      aaacgttta atggcaagg agtttagaa ctcaagttc 
      ggtcatttg gacgaggac tcggggact ttcattccg 
      ccgaagcag ttcctgcaa tatctcgtc aggatgggc 
      agcttcagc tcagcgccg aaaatccaa aatgacgat 
      aaccaagac aacgatatt caaataccg atcaacttt 
      ggccggttt gaactgctc cagtattgc cagacccag 
      ccccaagtc cagcccatg gtgaagagg gccttcaag 
      aaagcggga caaaccaac gacgatact tatggtttc 
      aagtgccgg aagttgaac gccgttacc aaaggttgt 
      agtgattac gtttctagt ccttactcg ttaagagtt 
      tttcagtgg aatatactc tcacaagcg ctgggacaa 
      atgaacgac catttcgtg aaatgtcca gacgaagcg 
      ttagaatgg aactcgcgg aaattccgg attattgag 
      gaaattgtc gagtattgt cccgatatt atttgtcta 
      caggaagtg gatcatttc aattttctg aagtacatc 
      ctcggcact caaggttac acgggggta ttttaccca 
      aaaccggac tcaccttgt gtttacatc agtggtaat 
      aatggtccc gatgggtgt gcaattttt tatcgaacc 
      aacaaattc gatgtaatt aacattgaa tcccgtatt 
      ttggaaatc tggagagta caaagcaat caagtggcc 
      cttttggcg aacctgcgc atcaaagag accggccag 
      gaggtctgt gtgaccacc acgcacttg aaggcccgc 
      caaggcgcc ttcctgtcc actttgcgc aacgaacaa 
      ggcaaggat ttgctccag tttgtgtcg caacattgc 
      ggcccccga cctgttgtc atctgcggc gattttaat 
      gcagaacct attgagccc atctacagt accatctta 
      tcggacgaa tatctaaat cttggtagt gcttacgct 
      gactgtgat agttcgagc gcgaattca gcggcgcga 
      gaaccccct tacaccacg tggaagata agggacgag 
      ggcgaagtc tgccacacc atagactat attttttac 
      aagaagggc tgtttagag gttgaagct gtgctggag 
      ttgcccacc ggggaggaa attggagag gatagggtt 
      ccctcgttt tcgtatcct tcggatcat ttttcgctc 
      gtttgcgat tttaagatt ggtcaaaag gcgacgctt 
      tga
    mRNA sequence
    • >TC003159 RA
      caattgtca acatctcat atcacacgg attgacacc 
      gaaaaaaaa gtactagag tacaactgc acattgacc 
      ggtcgttgg caagtgact tcatatcgt agataacga 
      tttttagtt cgtactagt ttccgttag accatcgaa 
      cagaaaatt cgtgtagtg tgtatcctt cgagtttta 
      ccaaatgcg gctctgaat gctctaaat tcgtgcaag 
      gtttccatc cggccttac ttgacatgc tggacacgc 
      tcaattttt taatgcaaa atcttccaa gtaaagtga 
      tgccattta agtggaatc ttgtgcaag tttacagta 
      gacattctt ttccttgta atttatagt aattagtgt 
      ttgaaacaa agtgaacgg aatggctgt tagaggggg 
      tcaattttt ctcaccgat aagaagatt tgctcgtat 
      gctggcctt accaaggat gggcagctt cagctcagc 
      gccgaaaat ccaaaatga cgataacca agacaacga 
      tattcaaat accgatcaa ctttggccg gtttgaact 
      gctccagta ttgccagac ccagcccca agtccagcc 
      catggtgaa gagggcctt caagaaagc gggacaaac 
      caacgacga tacttatgg tttcaagtg ccggaagtt 
      gaacgccgt taccaaagg ttgtagtga ttacgtttc 
      tagtcctta ctcgttaag agtttttca gtggaatat 
      actctcaca agcgctggg acaaatgaa cgaccattt 
      cgtgaaatg tccagacga agcgttaga atggaactc 
      gcggaaatt ccggattat tgaggaaat tgtcgagta 
      ttgtcccga tattatttg tctacagga agtggatca 
      tttcaattt tctgaagta catcctcgg cactcaagg 
      ttacacggg ggtatttta cccaaaacc ggactcacc 
      ttgtgttta catcagtgg taataatgg tcccgatgg 
      gtgtgcaat tttttatcg aaccaacaa attcgatgt 
      aattaacat tgaatcccg tattttgga aatctggag 
      agtacaaag caatcaagt ggccctttt ggcgaacct 
      gcgcatcaa agagaccgg ccaggaggt ctgtgtgac 
      caccacgca cttgaaggc ccgccaagg cgccttcct 
      gtccacttt gcgcaacga acaaggcaa ggatttgct 
      ccagtttgt gtcgcaaca ttgcggccc ccgacctgt 
      tgtcatctg cggcgattt taatgcaga acctattga 
      gcccatcta cagtaccat cttatcgga cgaatatct 
      aaatcttgg tagtgctta cgctgactg tgatagttc 
      gagcgcgaa ttcagcggc gcgagaacc cccttacac 
      cacgtggaa gataaggga cgagggcga agtctgcca 
      caccataga ctatatttt ttacaagaa gggctgttt 
      agaggttga agctgtgct ggagttgcc caccgggga 
      ggaaattgg agaggatag ggttccctc gttttcgta 
      tccttcgga tcatttttc gctcgtttg cgattttaa 
      gattggtca aaaggcgac gctttgatt tgtacattg 
      tgataatta attttaggg aagtgttgt gtaatgtct 
      gtaaattat ttgtgccta tttcttatg taagacctt 
      tttcaataa ctaatagga ttttaataa aattctaaa 
      caaacg
    • >TC003159 RB
      caattgtca acatctcat atcacacgg attgacacc 
      gaaaaaaaa gtactagag tacaactgc acattgacc 
      ggtcgttgg caagtgact tcatatcgt agataacga 
      tttttagtt cgtactagt ttccgttag accatcgaa 
      cagaaaatt cgtgtagtg tgtatcctt cgagtttta 
      ccaaatgcg gctctgaat gctctaaat tcgtgcaag 
      gtttccatc cggccttac ttgacatgc tggacacgc 
      tcaattttt taatgcaaa atcttccaa gtaaagtga 
      tgccattta agtggaatc ttgtgcaag tttacagta 
      gacattctt ttccttgta atttatagt aattagtgt 
      ttgaaacaa agtgaacgg aatggctgt tagaggggg 
      tcaattttt ctcaccgat aagaagatt tgctcgtat 
      gctggcctt accaagggc taacgtgcc agttggctt 
      caaaatgct agaatttga catcggtga ggaaaagat 
      cttaaaaaa tgttcgatt acagaaagt tattttgca 
      acggatggg cagcttcag ctcagcgcc gaaaatcca 
      aaatgacga taaccaaga caacgatat tcaaatacc 
      gatcaactt tggccggtt tgaactgct ccagtattg 
      ccagaccca gccccaagt ccagcccat ggtgaagag 
      ggccttcaa gaaagcggg acaaaccaa cgacgatac 
      ttatggttt caagtgccg gaagttgaa cgccgttac 
      caaaggttg tagtgatta cgtttctag tccttactc 
      gttaagagt ttttcagtg gaatatact ctcacaagc 
      gctgggaca aatgaacga ccatttcgt gaaatgtcc 
      agacgaagc gttagaatg gaactcgcg gaaattccg 
      gattattga ggaaattgt cgagtattg tcccgatat 
      tatttgtct acaggaagt ggatcattt caattttct 
      gaagtacat cctcggcac tcaaggtta cacgggggt 
      attttaccc aaaaccgga ctcaccttg tgtttacat 
      cagtggtaa taatggtcc cgatgggtg tgcaatttt 
      ttatcgaac caacaaatt cgatgtaat taacattga 
      atcccgtat tttggaaat ctggagagt acaaagcaa 
      tcaagtggc ccttttggc gaacctgcg catcaaaga 
      gaccggcca ggaggtctg tgtgaccac cacgcactt 
      gaaggcccg ccaaggcgc cttcctgtc cactttgcg 
      caacgaaca aggcaagga tttgctcca gtttgtgtc 
      gcaacattg cggcccccg acctgttgt catctgcgg 
      cgattttaa tgcagaacc tattgagcc catctacag 
      taccatctt atcggacga atatctaaa tcttggtag 
      tgcttacgc tgactgtga tagttcgag cgcgaattc 
      agcggcgcg agaaccccc ttacaccac gtggaagat 
      aagggacga gggcgaagt ctgccacac catagacta 
      tatttttta caagaaggg ctgtttaga ggttgaagc 
      tgtgctgga gttgcccac cggggagga aattggaga 
      ggatagggt tccctcgtt ttcgtatcc ttcggatca 
      tttttcgct cgtttgcga ttttaagat tggtcaaaa 
      ggcgacgct ttgatttgt acattgtga taattaatt 
      ttagggaag tgttgtgta atgtctgta aattatttg 
      tgcctattt cttatgtaa gaccttttt caataacta 
      ataggattt taataaaat tctaaacaa acg
    • >TC003159 RC
      caattgtca acatctcat atcacacgg attgacacc 
      gaaaaaaaa gtactagag tacaactgc acattgacc 
      ggtcgttgg caagtgact tcatatcgt agataacga 
      tttttagtt cgtactagt ttccgttag accatcgaa 
      cagaaaatt cgtgtagtg tgtatcctt cgagtttta 
      ccaaatgcg gctctgaat gctctaaat tcgtgcaag 
      gtttccatc cggccttac ttgacatgc tgatgcagt 
      agtgtaaag gtagatgaa cccgctttt tacgaacac 
      gtaaaaaaa tgagtgaga tatggcaat cgcaagacg 
      gtaccatca acatccggg agtctctgg cacttgcag 
      agactctat ccaaaatta gcctgatga catctccag 
      cagtcaaga aacgtttaa tggcaagga gtttagaac 
      tcaagttcg gtcatttgg acgaggact cggggactt 
      tcattccgc cgaagcagt tcctgcaat atctcgtca 
      ggatgggca gcttcagct cagcgccga aaatccaaa 
      atgacgata accaagaca acgatattc aaataccga 
      tcaactttg gccggtttg aactgctcc agtattgcc 
      agacccagc cccaagtcc agcccatgg tgaagaggg 
      ccttcaaga aagcgggac aaaccaacg acgatactt 
      atggtttca agtgccgga agttgaacg ccgttacca 
      aaggttgta gtgattacg tttctagtc cttactcgt 
      taagagttt ttcagtgga atatactct cacaagcgc 
      tgggacaaa tgaacgacc atttcgtga aatgtccag 
      acgaagcgt tagaatgga actcgcgga aattccgga 
      ttattgagg aaattgtcg agtattgtc ccgatatta 
      tttgtctac aggaagtgg atcatttca attttctga 
      agtacatcc tcggcactc aaggttaca cgggggtat 
      tttacccaa aaccggact caccttgtg tttacatca 
      gtggtaata atggtcccg atgggtgtg caatttttt 
      atcgaacca acaaattcg atgtaatta acattgaat 
      cccgtattt tggaaatct ggagagtac aaagcaatc 
      aagtggccc ttttggcga acctgcgca tcaaagaga 
      ccggccagg aggtctgtg tgaccacca cgcacttga 
      aggcccgcc aaggcgcct tcctgtcca ctttgcgca 
      acgaacaag gcaaggatt tgctccagt ttgtgtcgc 
      aacattgcg gcccccgac ctgttgtca tctgcggcg 
      attttaatg cagaaccta ttgagccca tctacagta 
      ccatcttat cggacgaat atctaaatc ttggtagtg 
      cttacgctg actgtgata gttcgagcg cgaattcag 
      cggcgcgag aaccccctt acaccacgt ggaagataa 
      gggacgagg gcgaagtct gccacacca tagactata 
      ttttttaca agaagggct gtttagagg ttgaagctg 
      tgctggagt tgcccaccg gggaggaaa ttggagagg 
      atagggttc cctcgtttt cgtatcctt cggatcatt 
      tttcgctcg tttgcgatt ttaagattg gtcaaaagg 
      cgacgcttt gatttgtac attgtgata attaatttt 
      agggaagtg ttgtgtaat gtctgtaaa ttatttgtg 
      cctatttct tatgtaaga cctttttca ataactaat 
      aggatttta ataaaattc taaacaaac g
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