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Gene information
General information
TC003143 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid14: 123142..127924
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003143
    iBeetle screen iB_00522
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003143 RA
      MVSSDEVPL IGVIDAGTR TVQFCVFRS QHTQEIAGH 
      AIDITQHTP QEGWFEEDP NEILQAVKT CMKNVVSQI 
      GDKACKNIV TIGITNQRE TTVVWDKTT GQPLCNAIV 
      WNDIRTDST VDIILAKVP ENNKNYFKP LCGLPISPY 
      FSAFKLKWL MHHVPEVKK AIKAKKCLF GTVDTWLLW 
      NLTGGPNGG LHITDVTNA SRTFLMNIE TFHWDPLLL 
      HTFKIPEEI LPEIRSSSE IYGKICGKD FPLQEVPIS 
      GILGNQQSA LVGQNCFKE GQAKNTYRS GCFLLYNTG 
      TNRVQSSHG LVTTVAYKF GDSPPVYAL EGSVAVAGS 
      ALSWLRDNM GFLKDVHQD TEHLAKEVF NTGDVYFVP 
      AFTGLYAPY WRKDARGII CGLTAFSTK QHIIRAALE 
      AICFQTRDI LEAMNKDCG IPLTKLNVD GRLTRNDLL 
      MQLQADISG IPVIRAQSQ DITALGVAI AAGQAKGIE 
      VWDLNAEDR EVIPSDTFL PTTTEDERD SRYTKWKMA 
      VQRSLGWAV PKKTFTMTE ERYRLLASI PGSLYIIGS 
      FGLLALSEY LARAS
    CDS sequence
    • >TC003143 RA
      atggtttct tcagatgag gtgccccta attggagtg 
      atagacgcc ggcactagg acagtccag ttttgtgtt 
      tttcgcagt cagcacact caggaaatc gcgggacat 
      gccatagat ataactcaa catacgcct caggaaggg 
      tggtttgaa gaagacccc aatgagatt cttcaggcg 
      gtgaaaacc tgcatgaag aacgttgtg agccagata 
      ggagataaa gcctgtaaa aacattgtc actatcgga 
      attaccaac cagcgggaa acgacagtt gtttgggat 
      aaaacaaca ggacagcct ttatgtaac gcaatcgtt 
      tggaacgat atcaggacg gactccacc gtcgacata 
      attctagca aaagtacct gaaaacaat aaaaactac 
      tttaagcca ctttgcgga cttccaatc tcgccgtat 
      ttcagcgcg tttaagttg aaatggctt atgcaccac 
      gtgcctgaa gtaaagaaa gcgataaag gcgaaaaag 
      tgccttttt ggcaccgtt gacacctgg ttattatgg 
      aacctaacg gggggtcca aacggtgga ctgcacatc 
      acagacgtc acaaacgcc tcaagaaca ttcctaatg 
      aatatagag acttttcac tgggatccg ctgcttttg 
      cacactttc aaaattccg gaagaaata ctcccggaa 
      attcggagc agttcggaa atttatgga aaaatttgc 
      ggaaaagac tttcctctg caagaagtg ccgatttca 
      gggatttta ggcaaccaa cagtcggct ctcgtaggt 
      caaaattgt ttcaaggaa ggtcaagca aaaaatacc 
      taccgtagc ggatgtttc cttttgtat aacacggga 
      acaaacaga gtacaatcg agtcatggc ttggtcacg 
      accgttgcg tacaaattc ggcgattct ccgcctgtt 
      tatgcctta gaaggtagc gttgctgtg gccggttcg 
      gccttatct tggctccga gacaatatg ggtttttta 
      aaagacgtc caccaagac accgaacac ctcgccaaa 
      gaagtgttc aacacgggg gatgtttat tttgtccct 
      gccttcacg ggtctttat gcaccttac tggcggaaa 
      gatgctaga gggataata tgcgggttg actgccttt 
      tccacgaag caacacatc atccgagcg gcattagag 
      gcgatctgt ttccaaact agggatatt ttagaagcc 
      atgaataaa gattgcggt attccatta acgaagtta 
      aacgtggac gggagatta acaagaaac gatttattg 
      atgcagtta caagctgat attagtggg attcccgta 
      attcgggct cagagtcag gatattacg gctttggga 
      gtcgctatt gcggctgga caagcaaaa gggatcgag 
      gtctgggat ttgaatgct gaagaccgt gaggttatt 
      cctagtgat acattcctc ccaacaacc acagaagat 
      gaaagggac tcgaggtac accaagtgg aagatggcc 
      gtccagagg tcgctaggt tgggccgtt ccgaagaaa 
      acattcacc atgaccgag gaaagatac cgacttttg 
      gctagcatt ccagggagt ttatacatt atcgggagt 
      tttggcctt ttggccttg tcagaatat ctcgcaaga 
      gcaagttaa
    mRNA sequence
    • >TC003143 RA
      attgttatc atcaccaat ctttatttt tttccccct 
      aaaaattcc gacatgtga attatgaat gtgaaattt 
      tgccgtata taagaaatg cgtgacttt ctcttggtt 
      catttaaaa ttggcgcat atttaaaat ttcgtctga 
      ttgccaaca tagcgagtt gcgcaattc ctacaagac 
      aatttggca cttgtcaaa aatttccga aatggtttt 
      atcagcaat ttgtattca tttgaaaaa taaatgatc 
      attaaacta ataaaattc ttatctaat cacaaatgg 
      agactagcg ccagcttat ttcgaattg ttcacatag 
      ttaagtgac gtagtggag gtgtgtgct tgcggttca 
      cagttttga aggttatat acgaaagaa atggtttct 
      tcagatgag gtgccccta attggagtg atagacgcc 
      ggcactagg acagtccag ttttgtgtt tttcgcagt 
      cagcacact caggaaatc gcgggacat gccatagat 
      ataactcaa catacgcct caggaaggg tggtttgaa 
      gaagacccc aatgagatt cttcaggcg gtgaaaacc 
      tgcatgaag aacgttgtg agccagata ggagataaa 
      gcctgtaaa aacattgtc actatcgga attaccaac 
      cagcgggaa acgacagtt gtttgggat aaaacaaca 
      ggacagcct ttatgtaac gcaatcgtt tggaacgat 
      atcaggacg gactccacc gtcgacata attctagca 
      aaagtacct gaaaacaat aaaaactac tttaagcca 
      ctttgcgga cttccaatc tcgccgtat ttcagcgcg 
      tttaagttg aaatggctt atgcaccac gtgcctgaa 
      gtaaagaaa gcgataaag gcgaaaaag tgccttttt 
      ggcaccgtt gacacctgg ttattatgg aacctaacg 
      gggggtcca aacggtgga ctgcacatc acagacgtc 
      acaaacgcc tcaagaaca ttcctaatg aatatagag 
      acttttcac tgggatccg ctgcttttg cacactttc 
      aaaattccg gaagaaata ctcccggaa attcggagc 
      agttcggaa atttatgga aaaatttgc ggaaaagac 
      tttcctctg caagaagtg ccgatttca gggatttta 
      ggcaaccaa cagtcggct ctcgtaggt caaaattgt 
      ttcaaggaa ggtcaagca aaaaatacc taccgtagc 
      ggatgtttc cttttgtat aacacggga acaaacaga 
      gtacaatcg agtcatggc ttggtcacg accgttgcg 
      tacaaattc ggcgattct ccgcctgtt tatgcctta 
      gaaggtagc gttgctgtg gccggttcg gccttatct 
      tggctccga gacaatatg ggtttttta aaagacgtc 
      caccaagac accgaacac ctcgccaaa gaagtgttc 
      aacacgggg gatgtttat tttgtccct gccttcacg 
      ggtctttat gcaccttac tggcggaaa gatgctaga 
      gggataata tgcgggttg actgccttt tccacgaag 
      caacacatc atccgagcg gcattagag gcgatctgt 
      ttccaaact agggatatt ttagaagcc atgaataaa 
      gattgcggt attccatta acgaagtta aacgtggac 
      gggagatta acaagaaac gatttattg atgcagtta 
      caagctgat attagtggg attcccgta attcgggct 
      cagagtcag gatattacg gctttggga gtcgctatt 
      gcggctgga caagcaaaa gggatcgag gtctgggat 
      ttgaatgct gaagaccgt gaggttatt cctagtgat 
      acattcctc ccaacaacc acagaagat gaaagggac 
      tcgaggtac accaagtgg aagatggcc gtccagagg 
      tcgctaggt tgggccgtt ccgaagaaa acattcacc 
      atgaccgag gaaagatac cgacttttg gctagcatt 
      ccagggagt ttatacatt atcgggagt tttggcctt 
      ttggccttg tcagaatat ctcgcaaga gcaagttaa 
      cgatattca ataagatga caattgacc taagtgaca 
      aaattggtg ttttgtagc tatttaatt ttaaacgat 
      ggcaaagga caagtagat tatttgttg atattttat 
      ctttattgt tgtgtactt aattttgtt gcttttttg 
      gttacaata aatcctgct cagtagt