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Gene information
General information
TC003132 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 1316578..1317955
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      		TC003132
      iBeetle screen iB_00521

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection

      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection

      adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
      Days post injection

      Fertility
      mature egg not present - Number of animals showing the phenotype: 4

      egg chamber number decreased - Number of animals showing the phenotype: 4
      vitellogenic egg chamber sometimes degenerated - Number of animals showing the phenotype: 4
      vitellarium partially thinner - Number of animals showing the phenotype: 4
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
      Days post injection

      pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 2 (11 dpi)
      Days post injection

      pupal molt delayed (still larva) - Number of animals showing the phenotype: 5 (11 dpi)
      Days post injection

      eclosion delayed (still larva) - Number of animals showing the phenotype: 5 (16 dpi)
      Days post injection

      eclosion delayed (still prepupa) - Number of animals showing the phenotype: 2 (16 dpi)
      Days post injection

      larva lethality late (L7) - Number of animals showing the phenotype: 5 (19 dpi)
      Days post injection

      pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 2 (19 dpi)
      Days post injection

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC003132 RA
        MDNTTQVQK YLVTFCKAL PAHVDCWRS 
ALQDSVKPL QNLANLCEQ QRAIQNVKL 
CNFGDYSDA QGVILHRIA IGIEEELLC 
IKKLVDKVN ECNIDLKNK LLVFERSTL 
NLNWEENNE LIRGNGSQP PLAKLLQYS 
LQFLNYFTK GAKDISDKL KVLNVKNVK 
SVKQLKNSF DVDLDYKIV KDLLALTQY 
VDNEKEIT
      CDS sequence
      • >TC003132 RA
        atggataat acaactcaa gtacaaaaa tatttggtt 
acgttttgt aaagctttg cctgcacat gttgattgt 
tggaggtcg gcattacag gattctgtc aagccgctc 
caaaacctg gctaatttg tgcgaacag cagcgggca 
attcaaaat gtgaaactg tgcaatttt ggggattat 
agtgatgca caaggtgtg atactgcat cgaatagcg 
ataggaatc gaggaggaa ttactttgc ataaaaaaa 
ttagttgac aaagtgaat gaatgtaac atagattta 
aaaaacaaa ttgttagtg tttgaaaga tctacgttg 
aatttaaac tgggaggaa aataatgag ctgatacgg 
gggaatgga tctcaacct cctttagcc aagttacta 
cagtacagt ttacagttt ttgaattat tttactaag 
ggagcaaaa gacattagt gataagtta aaagtatta 
aatgtaaaa aatgtaaag tcggttaag cagctaaaa 
aattctttt gatgttgat cttgattac aaaatagtt 
aaggactta ttagctcta acacaatat gttgacaat 
gaaaaagaa attacgtga
      mRNA sequence
      • >TC003132 RA
        aatggaagt tattaaaaa aatgaggac agtttgaat 
ttggcgcct gtggcttaa gtaaagtca tggctaccg 
cgttcgtgt ttttgtggt taggagctt caataaccg 
gtgaatgaa taaaactac gccaaggat taccttcct 
aagtacgtt ttgcgcttc gtaatggat aatacaact 
caagtacaa aaatatttg gttacgttt tgtaaagct 
ttgcctgca catgttgat tgttggagg tcggcatta 
caggattct gtcaagccg ctccaaaac ctggctaat 
ttgtgcgaa cagcagcgg gcaattcaa aatgtgaaa 
ctgtgcaat tttggggat tatagtgat gcacaaggt 
gtgatactg catcgaata gcgatagga atcgaggag 
gaattactt tgcataaaa aaattagtt gacaaagtg 
aatgaatgt aacatagat ttaaaaaac aaattgtta 
gtgtttgaa agatctacg ttgaattta aactgggag 
gaaaataat gagctgata cgggggaat ggatctcaa 
cctccttta gccaagtta ctacagtac agtttacag 
tttttgaat tattttact aagggagca aaagacatt 
agtgataag ttaaaagta ttaaatgta aaaaatgta 
aagtcggtt aagcagcta aaaaattct tttgatgtt 
gatcttgat tacaaaata gttaaggac ttattagct 
ctaacacaa tatgttgac aatgaaaaa gaaattacg 
tgacatttt tccaattcc aaaaaaacg agagattgt 
cttttacat ttaaaatat taaataatc aacacaaga 
aatcaccct tttattttg ctttaagct atgttattt 
attgatttt aaatcatgt aggtgtttt aatttttcc 
attctagtt agaaaactt agcttatac gagtgagcc 
agaaatttc ggcaaaata atttaacta attgggctt 
taaatacca aaaattgat tctggatat taaagaggg 
ataaggtct tttatggcc atttcgaaa aggagacga 
gcttaatat ataattctt ttaataaaa aaataattg 
gttccagta aaaattttg tgtattact ggttgcata 
cgtgtaaca ctactgaag tgtgagtaa aataaaaaa 
cgcggtttt tccacattt tcaaacatt acaaaatta 
aggaaatgt ttattattt aaaaaaata gttcagttt 
tattaaaaa attatattc tagtggtat tttagacta 
ataaagtac aaatatgaa a
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY