Status
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Gene information
General information
TC003132 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 1316578..1317955
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC003132
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
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      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
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      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
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      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
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      Name
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      Email
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      iBeetle screen iB_00521

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
      Days post injection


      Fertility
      mature egg not present - Number of animals showing the phenotype: 4

      egg chamber number decreased - Number of animals showing the phenotype: 4
      vitellogenic egg chamber sometimes degenerated - Number of animals showing the phenotype: 4
      vitellarium partially thinner - Number of animals showing the phenotype: 4
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
      Days post injection


      pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 2 (11 dpi)
      Days post injection


      pupal molt delayed (still larva) - Number of animals showing the phenotype: 5 (11 dpi)
      Days post injection


      eclosion delayed (still larva) - Number of animals showing the phenotype: 5 (16 dpi)
      Days post injection


      eclosion delayed (still prepupa) - Number of animals showing the phenotype: 2 (16 dpi)
      Days post injection


      larva lethality late (L7) - Number of animals showing the phenotype: 5 (19 dpi)
      Days post injection


      pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 2 (19 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC003132 RA
        MDNTTQVQK YLVTFCKAL PAHVDCWRS 
        ALQDSVKPL QNLANLCEQ QRAIQNVKL 
        CNFGDYSDA QGVILHRIA IGIEEELLC 
        IKKLVDKVN ECNIDLKNK LLVFERSTL 
        NLNWEENNE LIRGNGSQP PLAKLLQYS 
        LQFLNYFTK GAKDISDKL KVLNVKNVK 
        SVKQLKNSF DVDLDYKIV KDLLALTQY 
        VDNEKEIT
      CDS sequence
      • >TC003132 RA
        atggataat acaactcaa gtacaaaaa tatttggtt 
        acgttttgt aaagctttg cctgcacat gttgattgt 
        tggaggtcg gcattacag gattctgtc aagccgctc 
        caaaacctg gctaatttg tgcgaacag cagcgggca 
        attcaaaat gtgaaactg tgcaatttt ggggattat 
        agtgatgca caaggtgtg atactgcat cgaatagcg 
        ataggaatc gaggaggaa ttactttgc ataaaaaaa 
        ttagttgac aaagtgaat gaatgtaac atagattta 
        aaaaacaaa ttgttagtg tttgaaaga tctacgttg 
        aatttaaac tgggaggaa aataatgag ctgatacgg 
        gggaatgga tctcaacct cctttagcc aagttacta 
        cagtacagt ttacagttt ttgaattat tttactaag 
        ggagcaaaa gacattagt gataagtta aaagtatta 
        aatgtaaaa aatgtaaag tcggttaag cagctaaaa 
        aattctttt gatgttgat cttgattac aaaatagtt 
        aaggactta ttagctcta acacaatat gttgacaat 
        gaaaaagaa attacgtga
      mRNA sequence
      • >TC003132 RA
        aatggaagt tattaaaaa aatgaggac agtttgaat 
        ttggcgcct gtggcttaa gtaaagtca tggctaccg 
        cgttcgtgt ttttgtggt taggagctt caataaccg 
        gtgaatgaa taaaactac gccaaggat taccttcct 
        aagtacgtt ttgcgcttc gtaatggat aatacaact 
        caagtacaa aaatatttg gttacgttt tgtaaagct 
        ttgcctgca catgttgat tgttggagg tcggcatta 
        caggattct gtcaagccg ctccaaaac ctggctaat 
        ttgtgcgaa cagcagcgg gcaattcaa aatgtgaaa 
        ctgtgcaat tttggggat tatagtgat gcacaaggt 
        gtgatactg catcgaata gcgatagga atcgaggag 
        gaattactt tgcataaaa aaattagtt gacaaagtg 
        aatgaatgt aacatagat ttaaaaaac aaattgtta 
        gtgtttgaa agatctacg ttgaattta aactgggag 
        gaaaataat gagctgata cgggggaat ggatctcaa 
        cctccttta gccaagtta ctacagtac agtttacag 
        tttttgaat tattttact aagggagca aaagacatt 
        agtgataag ttaaaagta ttaaatgta aaaaatgta 
        aagtcggtt aagcagcta aaaaattct tttgatgtt 
        gatcttgat tacaaaata gttaaggac ttattagct 
        ctaacacaa tatgttgac aatgaaaaa gaaattacg 
        tgacatttt tccaattcc aaaaaaacg agagattgt 
        cttttacat ttaaaatat taaataatc aacacaaga 
        aatcaccct tttattttg ctttaagct atgttattt 
        attgatttt aaatcatgt aggtgtttt aatttttcc 
        attctagtt agaaaactt agcttatac gagtgagcc 
        agaaatttc ggcaaaata atttaacta attgggctt 
        taaatacca aaaattgat tctggatat taaagaggg 
        ataaggtct tttatggcc atttcgaaa aggagacga 
        gcttaatat ataattctt ttaataaaa aaataattg 
        gttccagta aaaattttg tgtattact ggttgcata 
        cgtgtaaca ctactgaag tgtgagtaa aataaaaaa 
        cgcggtttt tccacattt tcaaacatt acaaaatta 
        aggaaatgt ttattattt aaaaaaata gttcagttt 
        tattaaaaa attatattc tagtggtat tttagacta 
        ataaagtac aaatatgaa a
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY