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Gene information
General information
TC003123 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid14: 315007..317255
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003123
    iBeetle screen iB_00520
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003123 RA
      MNSKILKGP KLKQAAPKK SLGQLFFDC ATLHGDKLC 
      QVDGTADAT ESYSSVKQR STRVAIALQ ERGITSKDV 
      IAFCTGNTL DTVIPILAT FYLGAKVAN LDPSLSVRQ 
      TQHLIALVS PKIIFVEEN AVELIENSL KQTSVKTEI 
      IVYGRSGKY TSLGDLIQP RKNEATFRP PGVDLNEVA 
      LIFFSSGTT GLPKAICHS HKTCMLMGC TLQEYDWGQ 
      AVLHFTTFY WITAVFMLI SSFMTGGYR LFLRSMDPE 
      ITLRTVEKY KVDNMFLAP ILVNNFMSL GGDVKYDTS 
      SLRIILCGG GPMSSVQQK LMSRLFQRS TILCVYGMT 
      EVGCISLFH DERDKEFAK TKPLSCGKP FYELDLKIV 
      DVESGQTVG TNQKGEIRV DTPCTFIKY YNADCSDVF 
      DEEGFLKTG DIGYYDEDG CLYVIDRIK EMFKYKSWH 
      IVPSLIEKT LTEHPAVKE AAVFGVPSG DDGEIPAAC 
      IVLKDGAKA TKEEIKKFM DENVSDRER LRGGIKFVT 
      SLPKTPTGK FIRKEIKNS YIEAL
    CDS sequence
    • >TC003123 RA
      atgaactcc aagatcctc aaaggtccg aaactaaaa 
      caggccgct cctaaaaaa agtctaggc cagctgttt 
      ttcgactgt gcaaccctc cacggcgat aaattatgc 
      caggttgac ggtacagcc gacgcaacg gaatcatac 
      tcaagtgta aaacaaaga agcacacgt gtggccata 
      gcactccaa gaacgaggc atcacctcc aaagatgtg 
      attgccttt tgtacagga aacacgctg gatacggtc 
      attccaatt ctggcaacg ttttatcta ggcgccaaa 
      gtcgcaaat ttggacccg agcctctca gtccggcaa 
      acccaacac ctcattgct ctagtttcc ccaaaaatt 
      atttttgtt gaagaaaat gcagtggag ttaatcgag 
      aacagtctt aagcaaaca agtgttaaa acggagata 
      attgtctat ggtcggagt gggaaatac actagcctt 
      ggggattta attcaaccg cgcaaaaat gaggccact 
      tttaggccc cctggagtt gatttgaac gaagttgcg 
      ctcattttt ttcagtagt gggactaca ggacttccg 
      aaagcgatt tgtcacagt cataagact tgcatgctg 
      atggggtgc actctacag gaatatgat tggggacaa 
      gcagtgctt cactttact acgttttat tggatcact 
      gccgtgttt atgttgatt tcctcgttt atgacaggt 
      ggatatcgc ttgttcctc cggtctatg gatccagaa 
      ataacttta cgaactgtt gaaaaatac aaagttgat 
      aatatgttt ctagcgcca attttggtt aataatttt 
      atgtcactt ggtggggac gtaaaatat gatacttcc 
      tctttgcgg attattctc tgtggcggt ggccccatg 
      tcttcagtt cagcaaaaa ctaatgtct cgcctgttt 
      caacgttca acaattctt tgcgtttat ggaatgact 
      gaagtcggt tgtatttca ctatttcat gatgaaaga 
      gataaagaa tttgctaaa accaaacca ctgtcctgt 
      ggtaaaccg ttctatgaa ctggatctc aaaatcgtt 
      gacgttgaa tcgggacaa actgtgggt acaaaccaa 
      aaaggcgaa attcgagtt gatacaccc tgtacgttt 
      attaaatac tacaacgct gattgttcc gatgttttt 
      gacgaagaa ggtttttta aaaactgga gatatcgga 
      tattatgat gaagacggt tgtctttac gttattgac 
      agaatcaag gaaatgttc aagtacaaa tcctggcat 
      attgtacca tcgcttatt gagaaaact ctaacagaa 
      catcctgct gttaaagaa gctgctgtt tttggtgtg 
      ccgtctgga gacgatggg gaaatacct gctgcttgt 
      atagttctc aaagatggt gctaaagct acaaaggag 
      gaaatcaaa aagtttatg gacgagaat gtttccgac 
      agagaacgg ttgagagga ggaattaaa tttgtaaca 
      agtttaccc aaaactcca acgggaaag tttataaga 
      aaagaaatc aaaaattcg tacatcgag gctttgtag
    mRNA sequence
    • >TC003123 RA
      tccgatcca gttaacatc cttgcccat caattaaaa 
      taaagataa aacacgtac taagatgac gatgccaaa 
      aataattta aacctcgtc gtaaacaaa ttaattcgc 
      gtataaaac aacgacatc tggcattta aatccaaat 
      cttcagtgt tgttgaaaa tgaactcca agatcctca 
      aaggtccga aactaaaac aggccgctc ctaaaaaaa 
      gtctaggcc agctgtttt tcgactgtg caaccctcc 
      acggcgata aattatgcc aggttgacg gtacagccg 
      acgcaacgg aatcatact caagtgtaa aacaaagaa 
      gcacacgtg tggccatag cactccaag aacgaggca 
      tcacctcca aagatgtga ttgcctttt gtacaggaa 
      acacgctgg atacggtca ttccaattc tggcaacgt 
      tttatctag gcgccaaag tcgcaaatt tggacccga 
      gcctctcag tccggcaaa cccaacacc tcattgctc 
      tagtttccc caaaaatta tttttgttg aagaaaatg 
      cagtggagt taatcgaga acagtctta agcaaacaa 
      gtgttaaaa cggagataa ttgtctatg gtcggagtg 
      ggaaataca ctagccttg gggatttaa ttcaaccgc 
      gcaaaaatg aggccactt ttaggcccc ctggagttg 
      atttgaacg aagttgcgc tcatttttt tcagtagtg 
      ggactacag gacttccga aagcgattt gtcacagtc 
      ataagactt gcatgctga tggggtgca ctctacagg 
      aatatgatt ggggacaag cagtgcttc actttacta 
      cgttttatt ggatcactg ccgtgttta tgttgattt 
      cctcgttta tgacaggtg gatatcgct tgttcctcc 
      ggtctatgg atccagaaa taactttac gaactgttg 
      aaaaataca aagttgata atatgtttc tagcgccaa 
      ttttggtta ataatttta tgtcacttg gtggggacg 
      taaaatatg atacttcct ctttgcgga ttattctct 
      gtggcggtg gccccatgt cttcagttc agcaaaaac 
      taatgtctc gcctgtttc aacgttcaa caattcttt 
      gcgtttatg gaatgactg aagtcggtt gtatttcac 
      tatttcatg atgaaagag ataaagaat ttgctaaaa 
      ccaaaccac tgtcctgtg gtaaaccgt tctatgaac 
      tggatctca aaatcgttg acgttgaat cgggacaaa 
      ctgtgggta caaaccaaa aaggcgaaa ttcgagttg 
      atacaccct gtacgttta ttaaatact acaacgctg 
      attgttccg atgtttttg acgaagaag gttttttaa 
      aaactggag atatcggat attatgatg aagacggtt 
      gtctttacg ttattgaca gaatcaagg aaatgttca 
      agtacaaat cctggcata ttgtaccat cgcttattg 
      agaaaactc taacagaac atcctgctg ttaaagaag 
      ctgctgttt ttggtgtgc cgtctggag acgatgggg 
      aaatacctg ctgcttgta tagttctca aagatggtg 
      ctaaagcta caaaggagg aaatcaaaa agtttatgg 
      acgagaatg tttccgaca gagaacggt tgagaggag 
      gaattaaat ttgtaacaa gtttaccca aaactccaa 
      cgggaaagt ttataagaa aagaaatca aaaattcgt 
      acatcgagg ctttgtagt ttagtttgt catgtagat 
      tttatttct gaaaataaa tgtactagt gctta