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Gene information
General information
TC003122 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid14: 336644..338465
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003122
    iBeetle screen iB_00519
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 5 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003122 RA
      MMRNNILQG AEFKPTLDN TSLGDFFFN YAEKNGNKI 
      CHIDADLDQ SETYSSVKQ RTTRLAINL KKKGINSKD 
      VVAFCSYNS LDNTIPIIS SLYLGAKVA NLDPTLSTR 
      QTKHLLSLV SPRLIFVGE ESVPLIEKC LSEANLNSE 
      IVVFGNSTK YDTFSDLLA PSLEEKTFR PEKVDIHDI 
      AVMFFSSGT TGLPKAICH SHYSFLQLT EISNQSGHE 
      TSCTLHFTT FYWISGMLM LSLMFLEGG TRVFARNME 
      GEQTLRMIE KYKITALFV APVYTYELT SVANPERFD 
      LSSFRCFLT GGTSMSIEQ FKKLSSYFP QTHVLFGYG 
      MSEVGIISA FHTERDKHF IKTKFGSCG KVVAQTTLK 
      IVNPDTEEV LGPNQKGEI RIKSAGLFK GYYKADSSQ 
      CFDKDGFLK SGDVGYYDA DGCLYVVER IKEMFKYLS 
      WHIVPSLIE AILLEHPAV KEAVVFGIP AGEKGEIPA 
      ACVVLKDKC CNVNKDEIL KFVEERVSE REKLRGGIT 
      FLNELPRTP TGKLIRNEV RNIVIKSM
    CDS sequence
    • >TC003122 RA
      atgatgcga aacaatata ttgcaaggt gcagaattc 
      aaacctact ttagacaac acaagttta ggcgatttc 
      ttctttaat tatgctgag aaaaacggc aataaaatt 
      tgccacata gatgctgat ctggatcag tccgaaacc 
      tactctagt gtcaaacag cgaacgact cgactagca 
      ataaacctg aagaagaaa gggataaat tcaaaagac 
      gtggtggct ttctgtagt tacaactct cttgataac 
      accataccg attatttcg tccttgtat ctgggagcc 
      aaagtcgca aatttagac cccacactg tcaactaga 
      caaacaaaa catttattg tctcttgtc tcacctcgc 
      cttatcttt gttggagaa gagtcagtt cctttgatt 
      gaaaaatgt ctaagtgag gcaaacttg aattcagaa 
      attgtagtt ttcggaaac tcgactaaa tatgatacg 
      ttttccgat ttactcgca ccttcttta gaagaaaag 
      acatttagg ccggaaaaa gtcgacatt catgacatt 
      gctgttatg ttcttcagc agcggtacc acaggcttg 
      cctaaagca atctgccac agtcattat agttttttg 
      cagctgacg gaaatatca aatcaaagt ggacacgag 
      accagttgt actttgcac tttacaact ttttactgg 
      atatctggg atgttaatg ctgtcgctt atgtttctg 
      gaaggtggg actagagtt tttgctcgc aacatggag 
      ggagaacaa actctgaga atgattgaa aagtacaaa 
      ataacggct ctttttgtc gctcctgtc tacacgtat 
      gagctcaca agtgtggca aatccggag agattcgat 
      ctttcctcc ttccgatgc tttctaacc ggaggtact 
      tccatgagc atcgaacag ttcaaaaaa ctgagctct 
      tatttcccg caaactcac gtcctgttc ggttatgga 
      atgagtgag gttggcata atctccgct tttcacaca 
      gaacgagac aaacatttc attaaaacc aagtttggt 
      tcctgtggg aaagttgtg gcccaaaca actcttaaa 
      attgttaat ccagacact gaggaagtg ctgggtccg 
      aaccaaaag ggagaaatt cgtatcaaa tcagctgga 
      ctttttaaa gggtactac aaggctgat agttcgcag 
      tgttttgat aaagacggg tttctcaaa tcgggggat 
      gtgggatat tacgatgcc gatggttgt ttatacgta 
      gtggagcga ataaaagaa atgttcaag tatctctct 
      tggcacatt gtgccatct ttgattgag gccattttg 
      ttggaacat ccggctgtt aaggaagct gtggttttt 
      ggaattccg gctggtgaa aaaggggaa attccagcg 
      gcttgtgtt gttttgaaa gacaagtgt tgcaacgtt 
      aacaaagac gagatttta aagtttgta gaagagcgc 
      gtttccgaa agggagaag cttcgaggc ggaattaca 
      tttttgaac gagttgcct aggacgccc actggaaaa 
      ttaataaga aatgaagtt cgcaatatt gtcataaag 
      tcgatgtaa
    mRNA sequence
    • >TC003122 RA
      cttccttct tgtaccttt aaagtgtca acatgatgc 
      gaaacaata tattgcaag gtgcagaat tcaaaccta 
      ctttagaca acacaagtt taggcgatt tcttcttta 
      attatgctg agaaaaacg gcaataaaa tttgccaca 
      tagatgctg atctggatc agtccgaaa cctactcta 
      gtgtcaaac agcgaacga ctcgactag caataaacc 
      tgaagaaga aagggataa attcaaaag acgtggtgg 
      ctttctgta gttacaact ctcttgata acaccatac 
      cgattattt cgtccttgt atctgggag ccaaagtcg 
      caaatttag accccacac tgtcaacta gacaaacaa 
      aacatttat tgtctcttg tctcacctc gccttatct 
      ttgttggag aagagtcag ttcctttga ttgaaaaat 
      gtctaagtg aggcaaact tgaattcag aaattgtag 
      ttttcggaa actcgacta aatatgata cgttttccg 
      atttactcg caccttctt tagaagaaa agacattta 
      ggccggaaa aagtcgaca ttcatgaca ttgctgtta 
      tgttcttca gcagcggta ccacaggct tgcctaaag 
      caatctgcc acagtcatt atagttttt tgcagctga 
      cggaaatat caaatcaaa gtggacacg agaccagtt 
      gtactttgc actttacaa ctttttact ggatatctg 
      ggatgttaa tgctgtcgc ttatgtttc tggaaggtg 
      ggactagag tttttgctc gcaacatgg agggagaac 
      aaactctga gaatgattg aaaagtaca aaataacgg 
      ctctttttg tcgctcctg tctacacgt atgagctca 
      caagtgtgg caaatccgg agagattcg atctttcct 
      ccttccgat gctttctaa ccggaggta cttccatga 
      gcatcgaac agttcaaaa aactgagct cttatttcc 
      cgcaaactc acgtcctgt tcggttatg gaatgagtg 
      aggttggca taatctccg cttttcaca cagaacgag 
      acaaacatt tcattaaaa ccaagtttg gttcctgtg 
      ggaaagttg tggcccaaa caactctta aaattgtta 
      atccagaca ctgaggaag tgctgggtc cgaaccaaa 
      agggagaaa ttcgtatca aatcagctg gacttttta 
      aagggtact acaaggctg atagttcgc agtgttttg 
      ataaagacg ggtttctca aatcggggg atgtgggat 
      attacgatg ccgatggtt gtttatacg tagtggagc 
      gaataaaag aaatgttca agtatctct cttggcaca 
      ttgtgccat ctttgattg aggccattt tgttggaac 
      atccggctg ttaaggaag ctgtggttt ttggaattc 
      cggctggtg aaaaagggg aaattccag cggcttgtg 
      ttgttttga aagacaagt gttgcaacg ttaacaaag 
      acgagattt taaagtttg tagaagagc gcgtttccg 
      aaagggaga agcttcgag gcggaatta catttttga 
      acgagttgc ctaggacgc ccactggaa aattaataa 
      gaaatgaag ttcgcaata ttgtcataa agtcgatgt 
      aaaaaacat gttatgtta ttcggaaca ataaagtat 
      aatcgagta t