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Gene information
General information
TC003116 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 1433521..1436946
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003116
    iBeetle screen iB_00518

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    procephalic appendages sometimes remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
    gnathal appendages sometimes remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax segment partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax shortened - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen shortened - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    Fertility
    egg production: number <50% of expected -
    mature egg not present - Number of animals showing the phenotype: 4

    iBeetle screen iB_09736

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Adult morphology
    abdominal odoriferous glands colour decreased - Number of animals showing the phenotype: 3 (13 dpi)
    Days post injection



    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003116 RA
      MATKRRPIH TKLNFRPNI SDSNLECRG TKPTCEPTS 
      VRETFTLMV LYLCKKSLF FDTNLKVAI YLGTLFLLS 
      LVADVATIP KLYLSRSDN LFNKYFVKF AWGWNLLLL 
      TPFVVFTSY IYCCGQKKL IIKHHLLRI CIATFFWWL 
      WTSLFNVIE ASFGRCGQR QFSTKGTCL QAGHVWNGF 
      DISGHTFIL IYGSLFLIE ETRCIENWD SIKEHLRLE 
      NHNRTSRDP AENTNPLRN LSDDEMREL QFNYTKYTP 
      YIRALLMAI TLLQILWDV MLVCTMLYY HIMIEKFLG 
      GVLAILTWF FTYRMWYVH PKIIPNLPG QGVFKYIKK 
      KGEPQSVPR RRTGSIVNG RGPLFMGRP IYPQQDAQQ 
      DENSG
    CDS sequence
    • >TC003116 RA
      atggcgaca aaacgtcgt cccatacat acaaagtta 
      aattttcgc cccaacata agtgacagc aacctggag 
      tgccgcggg acaaagcca acatgtgag ccaacttcc 
      gtgagagag actttcacc ttaatggtc ttgtactta 
      tgcaaaaaa tcacttttc ttcgacact aacctcaaa 
      gtggccatc tacctcgga accctattt ttattatca 
      ctggtagca gatgtggcc acaattccc aaactgtac 
      ctttcacgt tctgacaat ttgtttaat aaatatttc 
      gtgaaattt gcctggggc tggaacctt ttactccta 
      accccgttt gttgtgttc acttcgtac atctactgt 
      tgcggacaa aagaaactc ataatcaag caccacctg 
      ctccgaatt tgcatcgcc acgttcttc tggtggttg 
      tggacaagt ctgttcaac gtgatcgag gcgtcgttt 
      ggacgctgc ggccaaaga cagtttagc accaagggc 
      acgtgtttg caagcgggg cacgtctgg aacggcttt 
      gacatctcg ggacacacc ttcattctc atttacggc 
      agcctcttc ctcattgaa gagacacga tgtatagaa 
      aactgggac agcatcaag gagcacctc agactggag 
      aaccacaac aggacgtcc agggacccc gcggaaaac 
      acaaacccg ttacggaat ttatcagac gacgaaatg 
      cgggaactc caattcaac tacaccaag tacacgccg 
      tacattagg gcgctcctt atggcaata actctgctt 
      cagattttg tgggacgtg atgcttgtg tgcacgatg 
      ttgtactac catatcatg attgagaag tttctcggg 
      ggagttttg gcaatttta acgtggttt ttcacctac 
      cggatgtgg tacgttcac ccgaagatc atccctaat 
      ttaccggga caaggggtt tttaaatac attaagaag 
      aagggggag ccccagtcc gtgcctagg agacgaact 
      gggagtata gtaaacggg cgggggcca cttttcatg 
      gggcgaccc atttatcca caacaggat gctcaacag 
      gacgaaaat tcagggtga
    mRNA sequence
    • >TC003116 RA
      gtggttatc ttatcagta ataaaaaat aaaacaaaa 
      atttatttt atattaact acatagctc ttacaatac 
      taatgaaca aatgctaaa agagttatt gttttttct 
      ttttcagta actccactc ccttgggtt taagtttat 
      tatcaagaa tcttttgct tcctgtcct cttcttctt 
      gtactagct tgttatgcc tctccactg caaagtcaa 
      tttctcctg ataatttaa tgatttgtt cagttaatt 
      cttgatttt tcataattc tctgttttt gtcctacac 
      cgtaagggg tgggccttc catccaaat aagaatagt 
      caggaccat ctgttagca ctccgcaac tgtttggat 
      ttaagggta agccccttt cttgtctcc atttctgat 
      ttaaataaa ttggcggag ttgtgccaa gacatcgct 
      ttgaagcag ataacaaaa ctaaaaacc aactattgt 
      tatctgtgc tgaaatatt taaaagaaa gagttacca 
      ttatttaaa tgtaggcat gacagcgat tttaaccac 
      attttttat acgaatttg ttactaaag gttgaggtt 
      agagcacga agaagttag gttaggatt agacaggaa 
      atggaaact aatcgcagg acttaatca cgatggagc 
      aagaaaaat tgttcttta tttacataa tagaaggag 
      ttctatcct atattcgta ttctaataa gtagcaatt 
      atcaaaatg tactgggcc ttaggaatg atcacaaaa 
      actgaaatg caatgagtg catcagaga cgctgtaaa 
      acttattac tacttacat aatttaaaa ttaaagctt 
      acataaatg tacaaacaa ctttggaaa atacatttt 
      ttacatccc cattaacat ttttttatg tagcacctc 
      ttcctcaaa cctttcata ttcactccc tatcattcc 
      caaatcaac attagcatc aacaacgaa aacaggcgc 
      tggacactc atacaacgt tttatggag caaccaatc 
      ttcatgaat ttcatgcaa actcctaat gtactcaaa 
      aggtactct tctttcttc agccctatc ccttttgac 
      aattctatc gtacacaac ctctggatc cgtcctcaa 
      atacacaaa catatcaac acccacatc ttgttcctt 
      tctgatcca tttaaacca ttcatcaat aactgaaaa 
      ttcgggtgc tgacagtaa ctcatctct cctcagttt 
      ctcaacaaa acaatatcg ggcgctata aatcgacct 
      ctccagcat tttaaaagg tgtgggagt tttcatagt 
      gtgtgcctg gagaagagt caactgcac atacgactg 
      gaaagtgaa gctgtttct tttcgggtc cttatacat 
      gaggtctag taaattgtg cccttgaca gttccgcca 
      cttatcgag cggttcagc cacgatggt aatgttgcc 
      caagtttcc catgtgctt taggaatgt tgtcttgcc 
      acttccgat gttgccctc aacggcaat agtgcaagg 
      acgttcact gaggcttcg tttcattgc actgcataa 
      tctcctaac gagcattcc ggtaatcta atccgagat 
      aatttatcc gcaaacttg tgcaatgtg ttgacagcc 
      acataaccc aactttgaa ttgtttata tttagggtt 
      aggtaactg tcacctgtc aatcgctca tgcgcattt 
      ttggagcaa acttgaaat gtgaaaatt acgcaacgg 
      gttcgaagt acttgttta aagtacttt ttttgtttt 
      tttcatttg actttgttt tatttacac attttaatc 
      gtagatttg ttagtgcct tgacatttg taacgtcag 
      taaaacgtc aaatttgac aagttataa gtcatcatg 
      gcgacaaaa cgtcgtccc atacataca aagttaaat 
      tttcgcccc aacataagt gacagcaac ctggagtgc 
      cgcgggaca aagccaaca tgtgagcca acttccgtg 
      agagagact ttcacctta atggtcttg tacttatgc 
      aaaaaatca cttttcttc gacactaac ctcaaagtg 
      gccatctac ctcggaacc ctattttta ttatcactg 
      gtagcagat gtggccaca attcccaaa ctgtacctt 
      tcacgttct gacaatttg tttaataaa tatttcgtg 
      aaatttgcc tggggctgg aacctttta ctcctaacc 
      ccgtttgtt gtgttcact tcgtacatc tactgttgc 
      ggacaaaag aaactcata atcaagcac cacctgctc 
      cgaatttgc atcgccacg ttcttctgg tggttgtgg 
      acaagtctg ttcaacgtg atcgaggcg tcgtttgga 
      cgctgcggc caaagacag tttagcacc aagggcacg 
      tgtttgcaa gcggggcac gtctggaac ggctttgac 
      atctcggga cacaccttc attctcatt tacggcagc 
      ctcttcctc attgaagag acacgatgt atagaaaac 
      tgggacagc atcaaggag cacctcaga ctggagaac 
      cacaacagg acgtccagg gaccccgcg gaaaacaca 
      aacccgtta cggaattta tcagacgac gaaatgcgg 
      gaactccaa ttcaactac accaagtac acgccgtac 
      attagggcg ctccttatg gcaataact ctgcttcag 
      attttgtgg gacgtgatg cttgtgtgc acgatgttg 
      tactaccat atcatgatt gagaagttt ctcggggga 
      gttttggca attttaacg tggtttttc acctaccgg 
      atgtggtac gttcacccg aagatcatc cctaattta 
      ccgggacaa ggggttttt aaatacatt aagaagaag 
      ggggagccc cagtccgtg cctaggaga cgaactggg 
      agtatagta aacgggcgg gggccactt ttcatgggg 
      cgacccatt tatccacaa caggatgct caacaggac 
      gaaaattca gggtgaatt ggggcctta ttcaagggt 
      ttggtgtgc ggttattgt tgtaattac gtaatatcg 
      atttttata tacgaagtt ttccacaaa aatttacat 
      taaatgaaa cgcattgtt ggattgttt tcgcatttg 
      cttgattta cataaggcg gttttaaaa agggacaat 
      ctgctgctc aaggcaaca gaacatcca ataatctgt 
      gatcacaac aatttttgt atgtattta caaaaaagt 
      aattgattt ttttgtata atttgcatt gtgtgtata 
      aattgcccg tcttggtca ttttatcct tattttaac 
      tcgtacagt gttcctgtt ttctctctt ttgtcacaa 
      agtacaatt ttgttgggt tgtttctaa tttattttt 
      gttactgat taaataact ttatgaacc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY