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Gene information
General information
TC032318
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC003112
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 1469449..1472696
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032318
    iBeetle screen iB_00517

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 3
    ovary without detectable defects - Number of animals showing the phenotype: 1
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 20
    5
    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032318 RA
      MRKFVFVLS LQFFLVKAI PAPDRFSGY SQYYDKNLS 
      NPALSFKEK ANVEKREAP PLDQFGFLS PYFSYDKEV 
      SNPTFNFEK HNHVKEQQE EKDKNVMTH ISTLPPFIW 
      EKFIQELNL GVNCSHNND NQSLETNAP TEAHMKNKN 
      STNLSPSSS EELTDEIVE TPKDNDSTN DLKNEDKTE 
      LSEADPTHS SNNLNKEKE TELPEADPT DPSNNLSEE 
      EETKLPELN PTDPSNSLN EEKEETELP EVNPTDPSN 
      NLSEEEETK LPELNPIDP SNNLNEEEE TKLPEADPT 
      NLSNNLSEE EETELPEAN PTDPSNNLS EEEETKLPE 
      LNPIDPSNN LNEEEKTKL PEADPTNLS NNLSEEEET 
      ELPEADQTD PSNTLSEEE ETELPELKP TDPSNNLNE 
      EEETELPEA DQTNLSSNA LDESSETTD TTIESKEEL 
      ESPEVDPTN LPGAFSSES NNANEITET PVASVIPLD 
      EDKLRSPEI SETTTASSE TSREDNESE KSSESPETS 
      NNDKDISKS PEESPAIVT DYFPNENVS KEIAIIYDL 
      HDAELNYNN SKKILESSK NIWKEIYGK EPSNVSILI 
      SSIDENDKK ADHITIDFD GETPRIKFV KEVGEIRKP 
      CLCSLSDEH
    CDS sequence
    • >TC032318 RA
      atgcgcaaa ttcgttttt gttttgtcg ttacaattt 
      tttctagta aaagctata ccagctcct gatagattt 
      agtggttat tctcaatac tatgacaaa aatctttca 
      aaccctgca ctcagtttt aaagaaaaa gctaacgta 
      gaaaaacga gaagcaccg cctcttgac caatttggc 
      tttctgtcc ccatatttt agctatgat aaagaagtg 
      tcaaatcca actttcaat tttgaaaaa cacaatcac 
      gtcaaagaa caacaagaa gaaaaagat aagaatgtt 
      atgacacat atttctacc ctccctcct tttatatgg 
      gaaaagttt atacaagaa ttaaactta ggggtaaac 
      tgctcccat aataatgat aatcagtca cttgaaact 
      aacgcacct acagaagca catatgaaa aataaaaat 
      tctacaaat ttatcacct agctcaagt gaagaatta 
      acagatgag attgtggaa acacctaaa gataatgat 
      tcaaccaat gatttaaaa aatgaagat aaaactgaa 
      ttatcagaa gctgatcca acccattca tctaataat 
      ttaaacaaa gaaaaagaa actgaattg ccagaagct 
      gatccaacc gatccttct aataattta agcgaagaa 
      gaagaaact aaattgcca gaacttaat ccaaccgat 
      ccttctaat agcttaaac gaagaaaaa gaagaaact 
      gaattgcca gaagttaat ccaaccgat ccttctaat 
      aatttaagc gaagaagaa gaaactaaa ttgccagaa 
      cttaatcca atcgatcct tctaataac ttaaacgaa 
      gaagaagaa actaaattg ccagaggct gatccaacc 
      aatttatct aataattta agcgaagaa gaagaaact 
      gaattgcca gaagctaat ccaaccgat ccttctaat 
      aatttaagc gaagaagaa gaaactaaa ttgccagaa 
      cttaatcca atcgatcct tctaataac ttaaacgaa 
      gaagaaaaa actaaattg ccagaggct gatccaacc 
      aatttatct aataattta agcgaagaa gaagaaact 
      gaattgcca gaagctgat caaaccgac ccttctaat 
      actttaagc gaagaagaa gaaactgaa ttgccagaa 
      cttaagcca accgatcct tctaataat ttaaacgaa 
      gaagaagaa actgaattg ccagaagct gatcaaacc 
      aacttatct tcaaatgca ttagatgaa tcttcagag 
      accacggat acaacaatc gaaagtaaa gaagaactt 
      gaatcacca gaagtcgat ccaactaat ttgcctggt 
      gcattctca agtgaatct aataacgct aacgaaatt 
      acagaaacg cccgtagct tccgtaata cctttagat 
      gaagacaaa ctgagatct ccagaaatt tcagaaaca 
      actacagct agtagtgaa acctcaaga gaagacaat 
      gaatcagaa aaatcatca gagtcgcca gaaacctca 
      aacaacgac aaagatatt agtaaatcc ccagaagaa 
      tcgcccgca atagtcact gactatttt cctaacgaa 
      aatgtttca aaagaaatt gctatcatt tatgacctc 
      cacgacgcc gaactcaac tacaataat tcgaaaaaa 
      atactagaa tcttctaaa aacatttgg aaagaaatt 
      tatggtaaa gaacccagt aatgttagt attttaatt 
      tcttctatc gatgaaaat gacaaaaaa gcagatcac 
      attactatt gactttgat ggggaaact ccaaggatc 
      aaatttgtt aaggaagtt ggagaaatt agaaaacct 
      tgcttatgt tcactcagt gatgaacat taa
    mRNA sequence
    • >TC032318 RA
      agaagaaat catgaaaat cctaatttt agtagcaac 
      agtatttct cgtatttgg tgttacaga tggtctaaa 
      ggcattcga agatctaga aaagtatgg aatggtttt 
      ggaactcat agaattgat taaaccaga atcctctca 
      tttgataga aaacgctga tcgtggtta cttaaatgc 
      tcgtatgtg ggatgacaa tacatatta ttaaaatgt 
      aatgcaata agtaatatg actcatctt tttatttaa 
      atacctgta attaaaata gcaagtggg tctataaag 
      tgaagataa caataaaca cagagcatg actaaaaat 
      cgcgtagag acaataaaa aatgcatgg aacacgatt 
      ttgtttata aaaaagatt attttttta tgtgtcttg 
      aattactta taaatgttg gaacaaaca aaattgttg 
      cacactaac aaaaatgcg caaattcgt ttttgtttt 
      gtcgttaca attttttct agtaaaagc tataccagc 
      tcctgatag atttagtgg ttattctca atactatga 
      caaaaatct ttcaaaccc tgcactcag ttttaaaga 
      aaaagctaa cgtagaaaa acgagaagc accgcctct 
      tgaccaatt tggctttct gtccccata ttttagcta 
      tgataaaga agtgtcaaa tccaacttt caattttga 
      aaaacacaa tcacgtcaa agaacaaca agaagaaaa 
      agataagaa tgttatgac acatatttc taccctccc 
      tccttttat atgggaaaa gtttataca agaattaaa 
      cttaggggt aaactgctc ccataataa tgataatca 
      gtcacttga aactaacgc acctacaga agcacatat 
      gaaaaataa aaattctac aaatttatc acctagctc 
      aagtgaaga attaacaga tgagattgt ggaaacacc 
      taaagataa tgattcaac caatgattt aaaaaatga 
      agataaaac tgaattatc agaagctga tccaaccca 
      ttcatctaa taatttaaa caaagaaaa agaaactga 
      attgccaga agctgatcc aaccgatcc ttctaataa 
      tttaagcga agaagaaga aactaaatt gccagaact 
      taatccaac cgatccttc taatagctt aaacgaaga 
      aaaagaaga aactgaatt gccagaagt taatccaac 
      cgatccttc taataattt aagcgaaga agaagaaac 
      taaattgcc agaacttaa tccaatcga tccttctaa 
      taacttaaa cgaagaaga agaaactaa attgccaga 
      ggctgatcc aaccaattt atctaataa tttaagcga 
      agaagaaga aactgaatt gccagaagc taatccaac 
      cgatccttc taataattt aagcgaaga agaagaaac 
      taaattgcc agaacttaa tccaatcga tccttctaa 
      taacttaaa cgaagaaga aaaaactaa attgccaga 
      ggctgatcc aaccaattt atctaataa tttaagcga 
      agaagaaga aactgaatt gccagaagc tgatcaaac 
      cgacccttc taatacttt aagcgaaga agaagaaac 
      tgaattgcc agaacttaa gccaaccga tccttctaa 
      taatttaaa cgaagaaga agaaactga attgccaga 
      agctgatca aaccaactt atcttcaaa tgcattaga 
      tgaatcttc agagaccac ggatacaac aatcgaaag 
      taaagaaga acttgaatc accagaagt cgatccaac 
      taatttgcc tggtgcatt ctcaagtga atctaataa 
      cgctaacga aattacaga aacgcccgt agcttccgt 
      aataccttt agatgaaga caaactgag atctccaga 
      aatttcaga aacaactac agctagtag tgaaacctc 
      aagagaaga caatgaatc agaaaaatc atcagagtc 
      gccagaaac ctcaaacaa cgacaaaga tattagtaa 
      atccccaga agaatcgcc cgcaatagt cactgacta 
      ttttcctaa cgaaaatgt ttcaaaaga aattgctat 
      catttatga cctccacga cgccgaact caactacaa 
      taattcgaa aaaaatact agaatcttc taaaaacat 
      ttggaaaga aatttatgg taaagaacc cagtaatgt 
      tagtatttt aatttcttc tatcgatga aaatgacaa 
      aaaagcaga tcacattac tattgactt tgatgggga 
      aactccaag gatcaaatt tgttaagga agttggaga 
      aattagaaa accttgctt atgttcact cagtgatga 
      acattaata acattttgc aattgttaa taaataact 
      aaagcctaa tcctcttaa ttttctgtt ttatttggc 
      atcatttaa aaaaaatac aaagataat attctttac 
      atttcgtta ccggtttca caaaatcca ttgtgaaaa 
      cttgtctgc tacaaattt gtttacaat attatcacg 
      acaatatgt attcataat aaagcatct ttgttcgtg 
      ctttgtggc aaagcaaaa gcttcaatg actttactt 
      aaaaaatgg aaaagttaa ataattatt ctttcgacg 
      ttcttcccg ttcacttcg agagttcaa tcgatatta 
      atttacatt tccgtcgag gaagatgga gttaattat 
      tcaaaaagt aaaacaagt atgtgcaca attcaccca 
      ataaattat cgatcgctg ttcccccaa tttttttcc 
      aatcgcgtg aacgaactt tactcgtcc ctactgtcc 
      cttgaatga tacgctttt accggaaag attgttcgg 
      tgtgatttc ctcaataat tcaatgacg ttttagata 
      aactaaact gaattacga tagtaatcg actgtgtta 
      ttaaaaata aataatccc ggattggct atctacatt 
      tcatatatt gattcgtgg actttgtta atggagaac 
      ggtgattga ccattgaac ttttcaaaa attaatgta 
      gcagtttca tgttttgta gattttgtt gtttactca 
      cactaatgc agcgatgtg tgaacacca tattgaaat 
      actgcccga ctaataaca ggctttgtt ggttattta 
      tgcatctac caaaaaatt taaaaagca tattagtct 
      ttgattac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY