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Gene information
General information
TC003103 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 1727774..1733990
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003103
    iBeetle screen iB_00516

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003103 RA
      MAPKLYIMQ LSPPCRAVL MVAKAIGLE 
      LDIEEVERE ALKTPEMLE LNPQHTVPI 
      LVDGDFVVW DSHAIAGYL VGQYAEDDT 
      LYPKDDIRK RAIIDQRLH FENGVLYER 
      CRAVAQILF SGIGDISED DRDKLLEAY 
      GFLEEFLNG HPWLAGDEM TVADLSVLA 
      TLASADLFI PVDSDRFPQ LSDWLKTGK 
      ELPYFDECN AEGLAKFKE MLPTPES
    CDS sequence
    • >TC003103 RA
      atggcacca aagctctac atcatgcag ctgagtcct 
      ccctgcagg gctgtgctg atggtggcc aaggccatc 
      ggtcttgaa cttgacatc gaggaggtc gagcgcgag 
      gccctaaag acgcccgag atgctggag ctcaacccc 
      cagcacacc gtgcctatt ctcgtggac ggcgatttc 
      gtcgtctgg gacagtcat gcgatcgct gggtatctg 
      gtaggtcag tacgccgag gacgacacc ctttatccc 
      aaggacgat atccgcaaa cgggcgatt attgatcag 
      aggctgcac ttcgaaaac ggggtgctc tacgagaga 
      tgtcgcgca gttgcccaa attcttttt tctggtatt 
      ggcgacatc tcggaagac gaccgcgac aaactgctt 
      gaagcttac ggcttcctg gaagagttc ctcaacggc 
      cacccctgg ctcgctggc gacgaaatg acagtggct 
      gacctcagc gtccttgcg actttagcc tcagccgac 
      ctctttatc cctgtggat tcagatcgc tttccccaa 
      ctctccgac tggttaaaa accggaaaa gagctgcct 
      tacttcgac gaatgcaat gccgaaggc ttggcaaag 
      ttcaaggag atgctccca actcccgaa tcgtaa
    mRNA sequence
    • >TC003103 RA
      ttcgacaat aaagagtac gcttgtcgg aactgttgg 
      aagacttat gcgaaaaaa tcgaccatg gcgagacat 
      atgaaggtc ccgtttcag atgtaacag gtgttgtcg 
      ttcgtacag gcattccca gtctttaag tagacacaa 
      aaagtttga gtgtggaaa agagcccct gttcagatg 
      tagtgttcc aaactgcaa gtttgtgtc caaagtcaa 
      gtgtaattt gaagcagca catgtgtag acatttaag 
      aaaggaatc gtgttataa atgatttaa taaaatttg 
      acttttttt ttcaaaaaa taacattgt ttggttgtg 
      ggcattttc tacagaggg tcttatttt aggcggaag 
      atttaagtg caagaattg caagaggtc gtacaaaaa 
      cggctgtag cttaagacg ccacatgaa gcacgaatg 
      tcagaagga gccaaagta caagtgtga cgaatgtgg 
      ccatcgaac taaatacag atcgagtct tttgtccca 
      tattgccaa cagacacaa attccctga gagcctcta 
      acgcctgcc attttcagg atcctacca gtgccccac 
      ttgcggaag aagatacaa agtggcgag gtctttgtg 
      gcgccacca aaagtacga gtgccaaaa agaaccgaa 
      atttaagtg ttttcattg cacttacag ggcgaagca 
      caaagccag tgtctttaa acatatgct ctgtgtaca 
      tcctaacat taacatgta aagctataa acgagttaa 
      aatcacaac tgactgttt ttattcctt tttacgaac 
      tgactttaa tttggaact atttcagac cgctactcg 
      tgtgaaaag tgtctcaaa tcttacact ttaaagcgg 
      aatctgaga cggcacatg aatgtcgag tgcggcaaa 
      gagagacac ctcaaatgt aaccggtgt aacgccagt 
      ttttattac aaacaggac ctgcggaaa cacttggcg 
      cgaatgcac ggacgctta atgtagctt gtttgccta 
      ataaatttg tacaaaaaa aaacatact ttttgatgc 
      gcccgtcct atcgatcct attaatcca tagaaaaag 
      ctaggcgtg acccagttt tacaaattc gatcgccca 
      cccagcaaa cgttaagct ttgtccttt caaggatca 
      gtcagtcag taacattca agctttcgt cgaatcgaa 
      gtgaaaaaa tggcaccaa agctctaca tcatgcagc 
      tgagtcctc cctgcaggg ctgtgctga tggtggcca 
      aggccatcg gtcttgaac ttgacatcg aggaggtcg 
      agcgcgagg ccctaaaga cgcccgaga tgctggagc 
      tcaaccccc agcacaccg tgcctattc tcgtggacg 
      gcgatttcg tcgtctggg acagtcatg cgatcgctg 
      ggtatctgg taggtcagt acgccgagg acgacaccc 
      tttatccca aggacgata tccgcaaac gggcgatta 
      ttgatcaga ggctgcact tcgaaaacg gggtgctct 
      acgagagat gtcgcgcag ttgcccaaa ttctttttt 
      ctggtattg gcgacatct cggaagacg accgcgaca 
      aactgcttg aagcttacg gcttcctgg aagagttcc 
      tcaacggcc acccctggc tcgctggcg acgaaatga 
      cagtggctg acctcagcg tccttgcga ctttagcct 
      cagccgacc tctttatcc ctgtggatt cagatcgct 
      ttccccaac tctccgact ggttaaaaa ccggaaaag 
      agctgcctt acttcgacg aatgcaatg ccgaaggct 
      tggcaaagt tcaaggaga tgctcccaa ctcccgaat 
      cgtaattct gtcacgatg gttagtcac aataaaccg 
      atatttcaa ta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY