Status
Please wait ...
Gene information
General information
TC003094 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 1876986..1886340
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003094
    iBeetle screen iB_00514

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 6 (19 dpi)
    Days post injection


    Pupal morphology
    antenna orientation irregular - Number of animals showing the phenotype: 8

    wings shortened - Number of animals showing the phenotype: 8

    wings blistered - Number of animals showing the phenotype: 8
    legs orientation irregular - Number of animals showing the phenotype: 7
    oblique dorsal thoracic muscles fragmented - Number of animals showing the phenotype: 6

    female genital lobes rounded - Number of animals showing the phenotype: 8

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003094 RA
      MSKVKVSNE WKRHVKQEY IRLRQQKRF KRADDVKLA 
      WNQNRIKMT ENLIAEQKR WTESKAFWV PLPEIPSHV 
      TCMKKAEVI GNEGDIQVT PIRIINAVT PIPTMYTWA 
      PIQQNFMVE DETVLHNIP YMGDDLLDQ DGTFIEELI 
      KNYDGKVHG DRESGFIDD ELFVELVHA LMQYQDSDK 
      GEKKDKDKE KTKEEEKKK GLDFPAFII FQAISSQFP 
      DKGDPEELR EKYIELTER TDPNAPPEC TPNIDGPKA 
      ASVPREQTM HSFHTLFCR RCFKYDCFL HRLQACHPG 
      PNLQKRRGP DLKPFTEPC SADCYMLLE SVKERMAAK 
      AKQEEEAKG KSVTEAGSS TEAKTNGNS NPRKVCKQQ 
      SVDSGNEAS SEDSNDSNK YKDSTDQDQ VSTTTSFSL 
      LGLMGANDH KEWTGSDES LFRGLHKIF LNNYCAIAQ 
      IMLTKTCQQ VYEFAQKED ADIPDEEAM RDYTPPRKK 
      KKKHRLWSV HCRKIQLKK ESNSNHVYN FTPCDHPGQ 
      SCDTQCPCI GAQNFCEKF CQCSSDCQN RFPGCRCKA 
      QCNTKQCPC YLAVRECDP DLCQTCGAD QFDISKITC 
      KNVSVQRGL HKHLLMAPS DVAGWGIFL KDSAQKNEF 
      ISEYCGEII SQDEADRRG KVYDKYMCS FLFNLNNDF 
      VVDATRKGN KIRFANHSI NPNCYAKVM MVNGDHRIG 
      IFAKRAIQP GEELFFDYR YGPTEQLKF VGIEREMEF 
      L
    CDS sequence
    • >TC003094 RA
      atgtcgaaa gtgaaagtg agtaacgaa tggaagcgt 
      cacgtgaag caagaatac atccgtttg agacaacaa 
      aagaggttc aaaagagcg gacgacgtg aaactagca 
      tggaaccag aaccggatc aaaatgacc gaaaacctg 
      atcgccgag cagaagcgc tggactgag agcaaagcc 
      ttctgggtg cccctgccc gagatcccc tcgcatgtg 
      acttgcatg aaaaaggcg gaagttatc gggaatgaa 
      ggcgatata caggtcacc cccattcgg attatcaac 
      gcggtgact cccatcccg accatgtac acgtgggcc 
      cccatccag cagaacttc atggtggag gacgaaact 
      gtcctgcat aacattcca tacatgggc gatgattta 
      ctggaccag gacgggacg tttatcgaa gaacttatc 
      aaaaattac gatgggaaa gtgcacggg gaccgggag 
      tcgggattt atcgatgat gagcttttt gtggagctg 
      gtgcacgcg ttgatgcaa taccaggac agcgacaaa 
      ggggagaaa aaagacaag gacaaggaa aagaccaaa 
      gaagaggaa aagaaaaag gggctcgat tttccagct 
      tttattata ttccaagcg atttcgtcg cagtttccc 
      gataagggg gacccagag gagttgagg gaaaagtac 
      attgaactg accgaacgg accgacccc aacgcgccc 
      cctgaatgc accccaaat atcgacggt ccaaaggcc 
      gcatctgta cccagagaa caaacaatg cactcgttt 
      cacactttg ttctgtaga cgttgcttc aaatacgac 
      tgttttttg catcgtctt caagcgtgt catccaggg 
      cccaacttg cagaagcgt cgcggtcca gatttgaag 
      ccctttacg gagccctgt agcgccgat tgctacatg 
      ctgctggaa tctgttaaa gaaagaatg gctgcgaaa 
      gccaaacaa gaggaggaa gccaaaggt aagtcagtg 
      actgaagct gggtcttcc accgaagcc aaaacaaac 
      ggaaactcg aatcctcgc aaagtgtgc aagcaacag 
      agcgttgat tcgggaaat gaagcgagt tctgaagac 
      agcaatgac agcaacaaa tacaaagac tcaaccgac 
      caagaccag gtgtcgaca acgacttcg ttcagcctc 
      ctcggcttg atgggcgcc aacgaccac aaagagtgg 
      accggctcg gacgagagc ctcttccgc ggcctccac 
      aaaatcttc ctgaacaat tactgcgcc attgctcaa 
      atcatgtta accaaaacc tgccaacaa gtctacgag 
      ttcgcccaa aaggaggac gccgacatt cccgacgag 
      gaagccatg cgagactac acacctccc cggaagaag 
      aaaaagaag caccgcctc tggtcagtg cactgtcgc 
      aaaatccag ctcaagaaa gagtccaac agcaaccac 
      gtctacaac ttcacgccc tgcgaccac ccgggccag 
      agctgcgac acgcagtgc ccttgcatc ggcgcccag 
      aatttctgc gagaagttt tgccagtgc agctctgat 
      tgtcagaac aggtttcca ggatgcaga tgcaaggcc 
      cagtgtaac actaaacag tgtccttgt tacttagct 
      gtgagggaa tgcgaccct gacttatgc cagacttgc 
      ggagctgat cagttcgat ataagtaaa ataacttgc 
      aaaaacgtc agtgtccag aggggatta cataagcat 
      ttattgatg gcgccgtcg gatgttgcc ggttggggg 
      atttttctc aaagatagt gcacagaaa aatgaattt 
      atatcggag tattgcggc gagattatt tcccaggac 
      gaagccgat cgcagaggc aaagtctac gataaatac 
      atgtgcagt ttcttgttt aatttaaat aacgatttc 
      gtcgttgac gctacacgg aagggaaat aaaattcgt 
      ttcgcaaat cattcaatc aacccgaat tgttacgca 
      aaagttatg atggttaat ggggatcac aggattggg 
      atttttgcc aaacgggct attcaaccc ggcgaggag 
      cttttcttt gattacaga tatggccca accgagcaa 
      ctgaagttt gtcggcata gaacgggaa atggaattt 
      ctgtaa
    mRNA sequence
    • >TC003094 RA
      tatattttt ggttttttc tgtgtttat agaactcct 
      tccgttcca aaatgaatt gacccttcc ctcagttcg 
      cattcattg ttttctttc ttaattggc gtgaaaatg 
      tcgaaagtg aaagtgagt aacgaatgg aagcgtcac 
      gtgaagcaa gaatacatc cgtttgaga caacaaaag 
      aggttcaaa agagcggac gacgtgaaa ctagcatgg 
      aaccagaac cggatcaaa atgaccgaa aacctgatc 
      gccgagcag aagcgctgg actgagagc aaagccttc 
      tgggtgccc ctgcccgag atcccctcg catgtgact 
      tgcatgaaa aaggcggaa gttatcggg aatgaaggc 
      gatatacag gtcaccccc attcggatt atcaacgcg 
      gtgactccc atcccgacc atgtacacg tgggccccc 
      atccagcag aacttcatg gtggaggac gaaactgtc 
      ctgcataac attccatac atgggcgat gatttactg 
      gaccaggac gggacgttt atcgaagaa cttatcaaa 
      aattacgat gggaaagtg cacggggac cgggagtcg 
      ggatttatc gatgatgag ctttttgtg gagctggtg 
      cacgcgttg atgcaatac caggacagc gacaaaggg 
      gagaaaaaa gacaaggac aaggaaaag accaaagaa 
      gaggaaaag aaaaagggg ctcgatttt ccagctttt 
      attatattc caagcgatt tcgtcgcag tttcccgat 
      aagggggac ccagaggag ttgagggaa aagtacatt 
      gaactgacc gaacggacc gaccccaac gcgccccct 
      gaatgcacc ccaaatatc gacggtcca aaggccgca 
      tctgtaccc agagaacaa acaatgcac tcgtttcac 
      actttgttc tgtagacgt tgcttcaaa tacgactgt 
      tttttgcat cgtcttcaa gcgtgtcat ccagggccc 
      aacttgcag aagcgtcgc ggtccagat ttgaagccc 
      tttacggag ccctgtagc gccgattgc tacatgctg 
      ctggaatct gttaaagaa agaatggct gcgaaagcc 
      aaacaagag gaggaagcc aaaggtaag tcagtgact 
      gaagctggg tcttccacc gaagccaaa acaaacgga 
      aactcgaat cctcgcaaa gtgtgcaag caacagagc 
      gttgattcg ggaaatgaa gcgagttct gaagacagc 
      aatgacagc aacaaatac aaagactca accgaccaa 
      gaccaggtg tcgacaacg acttcgttc agcctcctc 
      ggcttgatg ggcgccaac gaccacaaa gagtggacc 
      ggctcggac gagagcctc ttccgcggc ctccacaaa 
      atcttcctg aacaattac tgcgccatt gctcaaatc 
      atgttaacc aaaacctgc caacaagtc tacgagttc 
      gcccaaaag gaggacgcc gacattccc gacgaggaa 
      gccatgcga gactacaca cctccccgg aagaagaaa 
      aagaagcac cgcctctgg tcagtgcac tgtcgcaaa 
      atccagctc aagaaagag tccaacagc aaccacgtc 
      tacaacttc acgccctgc gaccacccg ggccagagc 
      tgcgacacg cagtgccct tgcatcggc gcccagaat 
      ttctgcgag aagttttgc cagtgcagc tctgattgt 
      cagaacagg tttccagga tgcagatgc aaggcccag 
      tgtaacact aaacagtgt ccttgttac ttagctgtg 
      agggaatgc gaccctgac ttatgccag acttgcgga 
      gctgatcag ttcgatata agtaaaata acttgcaaa 
      aacgtcagt gtccagagg ggattacat aagcattta 
      ttgatggcg ccgtcggat gttgccggt tgggggatt 
      tttctcaaa gatagtgca cagaaaaat gaatttata 
      tcggagtat tgcggcgag attatttcc caggacgaa 
      gccgatcgc agaggcaaa gtctacgat aaatacatg 
      tgcagtttc ttgtttaat ttaaataac gatttcgtc 
      gttgacgct acacggaag ggaaataaa attcgtttc 
      gcaaatcat tcaatcaac ccgaattgt tacgcaaaa 
      gttatgatg gttaatggg gatcacagg attgggatt 
      tttgccaaa cgggctatt caacccggc gaggagctt 
      ttctttgat tacagatat ggcccaacc gagcaactg 
      aagtttgtc ggcatagaa cgggaaatg gaatttctg 
      taaccgttt ttttaataa attttttca tttatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY