Status
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Gene information
General information
TC003094 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 1876986..1886340
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC003094
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00514

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection

    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
     egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    pupal molt not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 6 (19 dpi)
    Days post injection

    Pupal morphology
    antenna orientation irregular - Number of animals showing the phenotype: 8

    wings shortened - Number of animals showing the phenotype: 8

    wings blistered - Number of animals showing the phenotype: 8
    legs orientation irregular - Number of animals showing the phenotype: 7
    oblique dorsal thoracic muscles fragmented - Number of animals showing the phenotype: 6

    female genital lobes rounded - Number of animals showing the phenotype: 8

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003094 RA
      MSKVKVSNE WKRHVKQEY IRLRQQKRF KRADDVKLA 
WNQNRIKMT ENLIAEQKR WTESKAFWV PLPEIPSHV 
TCMKKAEVI GNEGDIQVT PIRIINAVT PIPTMYTWA 
PIQQNFMVE DETVLHNIP YMGDDLLDQ DGTFIEELI 
KNYDGKVHG DRESGFIDD ELFVELVHA LMQYQDSDK 
GEKKDKDKE KTKEEEKKK GLDFPAFII FQAISSQFP 
DKGDPEELR EKYIELTER TDPNAPPEC TPNIDGPKA 
ASVPREQTM HSFHTLFCR RCFKYDCFL HRLQACHPG 
PNLQKRRGP DLKPFTEPC SADCYMLLE SVKERMAAK 
AKQEEEAKG KSVTEAGSS TEAKTNGNS NPRKVCKQQ 
SVDSGNEAS SEDSNDSNK YKDSTDQDQ VSTTTSFSL 
LGLMGANDH KEWTGSDES LFRGLHKIF LNNYCAIAQ 
IMLTKTCQQ VYEFAQKED ADIPDEEAM RDYTPPRKK 
KKKHRLWSV HCRKIQLKK ESNSNHVYN FTPCDHPGQ 
SCDTQCPCI GAQNFCEKF CQCSSDCQN RFPGCRCKA 
QCNTKQCPC YLAVRECDP DLCQTCGAD QFDISKITC 
KNVSVQRGL HKHLLMAPS DVAGWGIFL KDSAQKNEF 
ISEYCGEII SQDEADRRG KVYDKYMCS FLFNLNNDF 
VVDATRKGN KIRFANHSI NPNCYAKVM MVNGDHRIG 
IFAKRAIQP GEELFFDYR YGPTEQLKF VGIEREMEF 
L
    CDS sequence
    • >TC003094 RA
      atgtcgaaa gtgaaagtg agtaacgaa tggaagcgt 
cacgtgaag caagaatac atccgtttg agacaacaa 
aagaggttc aaaagagcg gacgacgtg aaactagca 
tggaaccag aaccggatc aaaatgacc gaaaacctg 
atcgccgag cagaagcgc tggactgag agcaaagcc 
ttctgggtg cccctgccc gagatcccc tcgcatgtg 
acttgcatg aaaaaggcg gaagttatc gggaatgaa 
ggcgatata caggtcacc cccattcgg attatcaac 
gcggtgact cccatcccg accatgtac acgtgggcc 
cccatccag cagaacttc atggtggag gacgaaact 
gtcctgcat aacattcca tacatgggc gatgattta 
ctggaccag gacgggacg tttatcgaa gaacttatc 
aaaaattac gatgggaaa gtgcacggg gaccgggag 
tcgggattt atcgatgat gagcttttt gtggagctg 
gtgcacgcg ttgatgcaa taccaggac agcgacaaa 
ggggagaaa aaagacaag gacaaggaa aagaccaaa 
gaagaggaa aagaaaaag gggctcgat tttccagct 
tttattata ttccaagcg atttcgtcg cagtttccc 
gataagggg gacccagag gagttgagg gaaaagtac 
attgaactg accgaacgg accgacccc aacgcgccc 
cctgaatgc accccaaat atcgacggt ccaaaggcc 
gcatctgta cccagagaa caaacaatg cactcgttt 
cacactttg ttctgtaga cgttgcttc aaatacgac 
tgttttttg catcgtctt caagcgtgt catccaggg 
cccaacttg cagaagcgt cgcggtcca gatttgaag 
ccctttacg gagccctgt agcgccgat tgctacatg 
ctgctggaa tctgttaaa gaaagaatg gctgcgaaa 
gccaaacaa gaggaggaa gccaaaggt aagtcagtg 
actgaagct gggtcttcc accgaagcc aaaacaaac 
ggaaactcg aatcctcgc aaagtgtgc aagcaacag 
agcgttgat tcgggaaat gaagcgagt tctgaagac 
agcaatgac agcaacaaa tacaaagac tcaaccgac 
caagaccag gtgtcgaca acgacttcg ttcagcctc 
ctcggcttg atgggcgcc aacgaccac aaagagtgg 
accggctcg gacgagagc ctcttccgc ggcctccac 
aaaatcttc ctgaacaat tactgcgcc attgctcaa 
atcatgtta accaaaacc tgccaacaa gtctacgag 
ttcgcccaa aaggaggac gccgacatt cccgacgag 
gaagccatg cgagactac acacctccc cggaagaag 
aaaaagaag caccgcctc tggtcagtg cactgtcgc 
aaaatccag ctcaagaaa gagtccaac agcaaccac 
gtctacaac ttcacgccc tgcgaccac ccgggccag 
agctgcgac acgcagtgc ccttgcatc ggcgcccag 
aatttctgc gagaagttt tgccagtgc agctctgat 
tgtcagaac aggtttcca ggatgcaga tgcaaggcc 
cagtgtaac actaaacag tgtccttgt tacttagct 
gtgagggaa tgcgaccct gacttatgc cagacttgc 
ggagctgat cagttcgat ataagtaaa ataacttgc 
aaaaacgtc agtgtccag aggggatta cataagcat 
ttattgatg gcgccgtcg gatgttgcc ggttggggg 
atttttctc aaagatagt gcacagaaa aatgaattt 
atatcggag tattgcggc gagattatt tcccaggac 
gaagccgat cgcagaggc aaagtctac gataaatac 
atgtgcagt ttcttgttt aatttaaat aacgatttc 
gtcgttgac gctacacgg aagggaaat aaaattcgt 
ttcgcaaat cattcaatc aacccgaat tgttacgca 
aaagttatg atggttaat ggggatcac aggattggg 
atttttgcc aaacgggct attcaaccc ggcgaggag 
cttttcttt gattacaga tatggccca accgagcaa 
ctgaagttt gtcggcata gaacgggaa atggaattt 
ctgtaa
    mRNA sequence
    • >TC003094 RA
      tatattttt ggttttttc tgtgtttat agaactcct 
tccgttcca aaatgaatt gacccttcc ctcagttcg 
cattcattg ttttctttc ttaattggc gtgaaaatg 
tcgaaagtg aaagtgagt aacgaatgg aagcgtcac 
gtgaagcaa gaatacatc cgtttgaga caacaaaag 
aggttcaaa agagcggac gacgtgaaa ctagcatgg 
aaccagaac cggatcaaa atgaccgaa aacctgatc 
gccgagcag aagcgctgg actgagagc aaagccttc 
tgggtgccc ctgcccgag atcccctcg catgtgact 
tgcatgaaa aaggcggaa gttatcggg aatgaaggc 
gatatacag gtcaccccc attcggatt atcaacgcg 
gtgactccc atcccgacc atgtacacg tgggccccc 
atccagcag aacttcatg gtggaggac gaaactgtc 
ctgcataac attccatac atgggcgat gatttactg 
gaccaggac gggacgttt atcgaagaa cttatcaaa 
aattacgat gggaaagtg cacggggac cgggagtcg 
ggatttatc gatgatgag ctttttgtg gagctggtg 
cacgcgttg atgcaatac caggacagc gacaaaggg 
gagaaaaaa gacaaggac aaggaaaag accaaagaa 
gaggaaaag aaaaagggg ctcgatttt ccagctttt 
attatattc caagcgatt tcgtcgcag tttcccgat 
aagggggac ccagaggag ttgagggaa aagtacatt 
gaactgacc gaacggacc gaccccaac gcgccccct 
gaatgcacc ccaaatatc gacggtcca aaggccgca 
tctgtaccc agagaacaa acaatgcac tcgtttcac 
actttgttc tgtagacgt tgcttcaaa tacgactgt 
tttttgcat cgtcttcaa gcgtgtcat ccagggccc 
aacttgcag aagcgtcgc ggtccagat ttgaagccc 
tttacggag ccctgtagc gccgattgc tacatgctg 
ctggaatct gttaaagaa agaatggct gcgaaagcc 
aaacaagag gaggaagcc aaaggtaag tcagtgact 
gaagctggg tcttccacc gaagccaaa acaaacgga 
aactcgaat cctcgcaaa gtgtgcaag caacagagc 
gttgattcg ggaaatgaa gcgagttct gaagacagc 
aatgacagc aacaaatac aaagactca accgaccaa 
gaccaggtg tcgacaacg acttcgttc agcctcctc 
ggcttgatg ggcgccaac gaccacaaa gagtggacc 
ggctcggac gagagcctc ttccgcggc ctccacaaa 
atcttcctg aacaattac tgcgccatt gctcaaatc 
atgttaacc aaaacctgc caacaagtc tacgagttc 
gcccaaaag gaggacgcc gacattccc gacgaggaa 
gccatgcga gactacaca cctccccgg aagaagaaa 
aagaagcac cgcctctgg tcagtgcac tgtcgcaaa 
atccagctc aagaaagag tccaacagc aaccacgtc 
tacaacttc acgccctgc gaccacccg ggccagagc 
tgcgacacg cagtgccct tgcatcggc gcccagaat 
ttctgcgag aagttttgc cagtgcagc tctgattgt 
cagaacagg tttccagga tgcagatgc aaggcccag 
tgtaacact aaacagtgt ccttgttac ttagctgtg 
agggaatgc gaccctgac ttatgccag acttgcgga 
gctgatcag ttcgatata agtaaaata acttgcaaa 
aacgtcagt gtccagagg ggattacat aagcattta 
ttgatggcg ccgtcggat gttgccggt tgggggatt 
tttctcaaa gatagtgca cagaaaaat gaatttata 
tcggagtat tgcggcgag attatttcc caggacgaa 
gccgatcgc agaggcaaa gtctacgat aaatacatg 
tgcagtttc ttgtttaat ttaaataac gatttcgtc 
gttgacgct acacggaag ggaaataaa attcgtttc 
gcaaatcat tcaatcaac ccgaattgt tacgcaaaa 
gttatgatg gttaatggg gatcacagg attgggatt 
tttgccaaa cgggctatt caacccggc gaggagctt 
ttctttgat tacagatat ggcccaacc gagcaactg 
aagtttgtc ggcatagaa cgggaaatg gaatttctg 
taaccgttt ttttaataa attttttca tttatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY