General information | |||
TC003094 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 1876986..1886340 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC003094 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_00514 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
pupal molt not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (11 dpi)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 6 (19 dpi)
wings shortened - Number of animals showing the phenotype: 8
wings blistered - Number of animals showing the phenotype: 8
legs orientation irregular - Number of animals showing the phenotype: 7
oblique dorsal thoracic muscles fragmented - Number of animals showing the phenotype: 6
female genital lobes rounded - Number of animals showing the phenotype: 8
- >TC003094 RA
MSKVKVSNE WKRHVKQEY IRLRQQKRF KRADDVKLA WNQNRIKMT ENLIAEQKR WTESKAFWV PLPEIPSHV TCMKKAEVI GNEGDIQVT PIRIINAVT PIPTMYTWA PIQQNFMVE DETVLHNIP YMGDDLLDQ DGTFIEELI KNYDGKVHG DRESGFIDD ELFVELVHA LMQYQDSDK GEKKDKDKE KTKEEEKKK GLDFPAFII FQAISSQFP DKGDPEELR EKYIELTER TDPNAPPEC TPNIDGPKA ASVPREQTM HSFHTLFCR RCFKYDCFL HRLQACHPG PNLQKRRGP DLKPFTEPC SADCYMLLE SVKERMAAK AKQEEEAKG KSVTEAGSS TEAKTNGNS NPRKVCKQQ SVDSGNEAS SEDSNDSNK YKDSTDQDQ VSTTTSFSL LGLMGANDH KEWTGSDES LFRGLHKIF LNNYCAIAQ IMLTKTCQQ VYEFAQKED ADIPDEEAM RDYTPPRKK KKKHRLWSV HCRKIQLKK ESNSNHVYN FTPCDHPGQ SCDTQCPCI GAQNFCEKF CQCSSDCQN RFPGCRCKA QCNTKQCPC YLAVRECDP DLCQTCGAD QFDISKITC KNVSVQRGL HKHLLMAPS DVAGWGIFL KDSAQKNEF ISEYCGEII SQDEADRRG KVYDKYMCS FLFNLNNDF VVDATRKGN KIRFANHSI NPNCYAKVM MVNGDHRIG IFAKRAIQP GEELFFDYR YGPTEQLKF VGIEREMEF L
- >TC003094 RA
atgtcgaaa gtgaaagtg agtaacgaa tggaagcgt cacgtgaag caagaatac atccgtttg agacaacaa aagaggttc aaaagagcg gacgacgtg aaactagca tggaaccag aaccggatc aaaatgacc gaaaacctg atcgccgag cagaagcgc tggactgag agcaaagcc ttctgggtg cccctgccc gagatcccc tcgcatgtg acttgcatg aaaaaggcg gaagttatc gggaatgaa ggcgatata caggtcacc cccattcgg attatcaac gcggtgact cccatcccg accatgtac acgtgggcc cccatccag cagaacttc atggtggag gacgaaact gtcctgcat aacattcca tacatgggc gatgattta ctggaccag gacgggacg tttatcgaa gaacttatc aaaaattac gatgggaaa gtgcacggg gaccgggag tcgggattt atcgatgat gagcttttt gtggagctg gtgcacgcg ttgatgcaa taccaggac agcgacaaa ggggagaaa aaagacaag gacaaggaa aagaccaaa gaagaggaa aagaaaaag gggctcgat tttccagct tttattata ttccaagcg atttcgtcg cagtttccc gataagggg gacccagag gagttgagg gaaaagtac attgaactg accgaacgg accgacccc aacgcgccc cctgaatgc accccaaat atcgacggt ccaaaggcc gcatctgta cccagagaa caaacaatg cactcgttt cacactttg ttctgtaga cgttgcttc aaatacgac tgttttttg catcgtctt caagcgtgt catccaggg cccaacttg cagaagcgt cgcggtcca gatttgaag ccctttacg gagccctgt agcgccgat tgctacatg ctgctggaa tctgttaaa gaaagaatg gctgcgaaa gccaaacaa gaggaggaa gccaaaggt aagtcagtg actgaagct gggtcttcc accgaagcc aaaacaaac ggaaactcg aatcctcgc aaagtgtgc aagcaacag agcgttgat tcgggaaat gaagcgagt tctgaagac agcaatgac agcaacaaa tacaaagac tcaaccgac caagaccag gtgtcgaca acgacttcg ttcagcctc ctcggcttg atgggcgcc aacgaccac aaagagtgg accggctcg gacgagagc ctcttccgc ggcctccac aaaatcttc ctgaacaat tactgcgcc attgctcaa atcatgtta accaaaacc tgccaacaa gtctacgag ttcgcccaa aaggaggac gccgacatt cccgacgag gaagccatg cgagactac acacctccc cggaagaag aaaaagaag caccgcctc tggtcagtg cactgtcgc aaaatccag ctcaagaaa gagtccaac agcaaccac gtctacaac ttcacgccc tgcgaccac ccgggccag agctgcgac acgcagtgc ccttgcatc ggcgcccag aatttctgc gagaagttt tgccagtgc agctctgat tgtcagaac aggtttcca ggatgcaga tgcaaggcc cagtgtaac actaaacag tgtccttgt tacttagct gtgagggaa tgcgaccct gacttatgc cagacttgc ggagctgat cagttcgat ataagtaaa ataacttgc aaaaacgtc agtgtccag aggggatta cataagcat ttattgatg gcgccgtcg gatgttgcc ggttggggg atttttctc aaagatagt gcacagaaa aatgaattt atatcggag tattgcggc gagattatt tcccaggac gaagccgat cgcagaggc aaagtctac gataaatac atgtgcagt ttcttgttt aatttaaat aacgatttc gtcgttgac gctacacgg aagggaaat aaaattcgt ttcgcaaat cattcaatc aacccgaat tgttacgca aaagttatg atggttaat ggggatcac aggattggg atttttgcc aaacgggct attcaaccc ggcgaggag cttttcttt gattacaga tatggccca accgagcaa ctgaagttt gtcggcata gaacgggaa atggaattt ctgtaa
- >TC003094 RA
tatattttt ggttttttc tgtgtttat agaactcct tccgttcca aaatgaatt gacccttcc ctcagttcg cattcattg ttttctttc ttaattggc gtgaaaatg tcgaaagtg aaagtgagt aacgaatgg aagcgtcac gtgaagcaa gaatacatc cgtttgaga caacaaaag aggttcaaa agagcggac gacgtgaaa ctagcatgg aaccagaac cggatcaaa atgaccgaa aacctgatc gccgagcag aagcgctgg actgagagc aaagccttc tgggtgccc ctgcccgag atcccctcg catgtgact tgcatgaaa aaggcggaa gttatcggg aatgaaggc gatatacag gtcaccccc attcggatt atcaacgcg gtgactccc atcccgacc atgtacacg tgggccccc atccagcag aacttcatg gtggaggac gaaactgtc ctgcataac attccatac atgggcgat gatttactg gaccaggac gggacgttt atcgaagaa cttatcaaa aattacgat gggaaagtg cacggggac cgggagtcg ggatttatc gatgatgag ctttttgtg gagctggtg cacgcgttg atgcaatac caggacagc gacaaaggg gagaaaaaa gacaaggac aaggaaaag accaaagaa gaggaaaag aaaaagggg ctcgatttt ccagctttt attatattc caagcgatt tcgtcgcag tttcccgat aagggggac ccagaggag ttgagggaa aagtacatt gaactgacc gaacggacc gaccccaac gcgccccct gaatgcacc ccaaatatc gacggtcca aaggccgca tctgtaccc agagaacaa acaatgcac tcgtttcac actttgttc tgtagacgt tgcttcaaa tacgactgt tttttgcat cgtcttcaa gcgtgtcat ccagggccc aacttgcag aagcgtcgc ggtccagat ttgaagccc tttacggag ccctgtagc gccgattgc tacatgctg ctggaatct gttaaagaa agaatggct gcgaaagcc aaacaagag gaggaagcc aaaggtaag tcagtgact gaagctggg tcttccacc gaagccaaa acaaacgga aactcgaat cctcgcaaa gtgtgcaag caacagagc gttgattcg ggaaatgaa gcgagttct gaagacagc aatgacagc aacaaatac aaagactca accgaccaa gaccaggtg tcgacaacg acttcgttc agcctcctc ggcttgatg ggcgccaac gaccacaaa gagtggacc ggctcggac gagagcctc ttccgcggc ctccacaaa atcttcctg aacaattac tgcgccatt gctcaaatc atgttaacc aaaacctgc caacaagtc tacgagttc gcccaaaag gaggacgcc gacattccc gacgaggaa gccatgcga gactacaca cctccccgg aagaagaaa aagaagcac cgcctctgg tcagtgcac tgtcgcaaa atccagctc aagaaagag tccaacagc aaccacgtc tacaacttc acgccctgc gaccacccg ggccagagc tgcgacacg cagtgccct tgcatcggc gcccagaat ttctgcgag aagttttgc cagtgcagc tctgattgt cagaacagg tttccagga tgcagatgc aaggcccag tgtaacact aaacagtgt ccttgttac ttagctgtg agggaatgc gaccctgac ttatgccag acttgcgga gctgatcag ttcgatata agtaaaata acttgcaaa aacgtcagt gtccagagg ggattacat aagcattta ttgatggcg ccgtcggat gttgccggt tgggggatt tttctcaaa gatagtgca cagaaaaat gaatttata tcggagtat tgcggcgag attatttcc caggacgaa gccgatcgc agaggcaaa gtctacgat aaatacatg tgcagtttc ttgtttaat ttaaataac gatttcgtc gttgacgct acacggaag ggaaataaa attcgtttc gcaaatcat tcaatcaac ccgaattgt tacgcaaaa gttatgatg gttaatggg gatcacagg attgggatt tttgccaaa cgggctatt caacccggc gaggagctt ttctttgat tacagatat ggcccaacc gagcaactg aagtttgtc ggcatagaa cgggaaatg gaatttctg taaccgttt ttttaataa attttttca tttatt
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
