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Gene information
General information
TC003079 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 2473235..2482351
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC003079
    iBeetle screen iB_00512

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 9 (9 dpi)
    Days post injection

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003079 RA
      MGSVWKRLQ RVNKRAAKF QFTVSYHQL AVEFTSKWQ 
PHKLSLVWS RRSRRVASA PMAWEPTLQ NAYKGLVVW 
PVPENHQVA ITLFRDPRT SELEDKEWS FIIEDVPTT 
GKRRQLAVA HVNMRKYAS IESTHSELQ ISFKTTTKK 
ITSAKLDCT LSCVLLREG KATDEDMQS MASLMSVNN 
NSDDIAALE DVEDDDFSL CQNDNSVKS SINEVTQHL 
QQMTNSLSG SDFASTPIS VASIQSFSR DDKTPTAEN 
FSPIGEKPK ATTVPPPRF SDFAEDDVD PYSEQCTEL 
LDKLDALEE DEESVPRPK SVNLKLKPL ELKEHIDLP 
KNQPQKLTT PGQDLLEWC KEMTKNYLG VKVTNLTTS 
WRNGMAFCA VIHHFEPSL IDFNSLSPH DVKGNCKIA 
FDAGDKLGI PRVIEPTDM HMLAVPDKL AVMTYLHQL 
RAHFTGHQL EVQQIGKTS EESNYVIGK FNTDKDTDI 
TKQVFGQEI INLRKAKQN QQLNKSQDS KKEKELNQT 
NKEISQNQD MKIAASKLQ LPLKITNEP AESPKEPKE 
KSPTVVKDM KDIILLGSK SIMSKVMSS PSKDKIQAK 
KTEVKKPIL MTRRELTDP FGSDDEEEV TDNKVINNK 
VPEADEMNG SGDHKVKEN QNDVFSDLP KPNAQILMR 
HSEQRERAR QLLEQTRKD ASITTSPTR SEEERQAQL 
RERARKILA EARRGSTPQ TEIIRLSPD SVKNFLEND 
EKSFLEGNA NFERQNSTG SEKNGNVYS EANSEKSSP 
SNRERITPD KLPDELGLK EMGIDVHNW IDKEMEDLE 
REQSAIDEQ AAVLEKQLR LVMESPNSN GEEEEALMA 
KWFMFVNKK NALLRRQMQ LNILEKEAD LETKYKMLN 
KELQKIASI EDWRKTEEQ RNREQMLLD ELVQIVNKR 
DELVHHLDN QEKAIEEDD LIEQDLSQL DLQPKEKCV 
IQ
    CDS sequence
    • >TC003079 RA
      atgggctct gtctggaaa cggctccag agagtgaat 
aaacgtgcg gcaaagttt cagttcacg gtgtcatat 
catcagctt gcagtagaa ttcacctca aaatggcaa 
ccccacaaa ctttcctta gtatggtca cgtaggtct 
agaagggtg gcttccgcc ccaatggct tgggagcca 
actcttcag aatgcttac aagggatta gtggtttgg 
ccggttcca gaaaatcat caagtagct attacttta 
tttagagac ccgagaact tcagaatta gaagacaag 
gaatggtca tttattata gaagatgtc ccaacgacc 
ggaaaaaga cgccaactt gccgttgca catgtcaat 
atgcgaaag tatgccagc atcgaatca actcactca 
gaattacaa atatcgttt aagactaca acaaagaaa 
attacttcc gccaaactt gactgcact ctatcgtgt 
gtcctttta cgcgaagga aaagcgaca gatgaagac 
atgcaatcg atggcgtct ctaatgtct gtaaataat 
aacagcgac gatattgcc gcgttagag gatgtagaa 
gacgacgat ttctctcta tgtcagaac gacaactcg 
gtcaaaagc agcattaac gaagtgacc caacattta 
caacaaatg accaacagc ttgtcgggc tccgacttt 
gccagtacg ccaattagc gttgcaagt attcagtcg 
ttttcacga gacgataaa actccaaca gctgagaat 
ttttcaccg attggggaa aaacccaaa gctacgact 
gttccacca cctagattt agcgacttt gctgaggat 
gatgtcgat ccgtattcg gaacaatgc accgaactg 
ttagataaa ttagacgcc ttggaggaa gacgaagag 
agtgtgcct agacctaag agtgtaaat ttgaagtta 
aaacctttg gaactgaag gaacatatt gacctgcca 
aagaaccaa ccccaaaaa ttgacgact ccagggcaa 
gatttatta gagtggtgt aaggaaatg acgaaaaat 
tatctcgga gttaaggta acgaattta acgacgagc 
tggaggaac ggaatggcc ttttgtgcc gtcatacat 
cattttgaa ccgagctta attgacttc aattctctt 
tcacctcac gatgtgaaa ggaaattgt aagatagcg 
tttgacgcc ggtgataag ttgggtatt ccgagggtg 
attgaaccg acagatatg cacatgttg gcggtgcct 
gataagttg gcagtaatg acttacttg caccaactg 
agggcacac tttacgggt caccagtta gaagtacaa 
caaatcggt aaaacttcc gaggaaagc aattatgtc 
attggaaaa ttcaatact gataaagac acagatatt 
acgaagcaa gtcttcggc caagagatt ataaattta 
aggaaagct aagcagaat caacagttg aataaatcg 
caagattcg aaaaaagag aaagagtta aaccaaacc 
aataaggaa attagccaa aatcaagac atgaaaatt 
gccgcttca aaactacaa cttccatta aaaatcacc 
aatgaaccg gctgaaagt ccgaaagag cccaaagaa 
aagtccccg actgttgtc aaagacatg aaagacatt 
atcttgtta ggttctaaa agcataatg agtaaagta 
atgtcttcg ccttcaaaa gacaaaatt caggctaag 
aaaactgaa gtgaagaaa ccgattctg atgacacga 
agggagctc acggacccg ttcgggtcc gatgatgag 
gaggaagtt actgataat aaagtcatc aataataaa 
gtacctgaa gctgatgag atgaacggt tctggtgat 
cataaagtt aaggaaaat caaaacgat gtgttttcg 
gatcttccc aaaccgaat gcgcaaata cttatgcga 
cattcagag caaagagaa cgtgccagg cagttgctt 
gaacagacc agaaaagac gcttccatc accacttcc 
cccactagg tctgaagag gaaagacaa gcgcaactg 
cgtgaacgt gcgagaaaa atactcgca gaagctcgg 
agagggtcc accccacaa actgagatt attcgactg 
agtccagac tcggtcaaa aacttttta gaaaatgac 
gagaaaagt ttccttgaa gggaatgcg aattttgaa 
cgacaaaat agcaccggt tctgaaaag aacggtaat 
gtttattcg gaagctaat tcagaaaag tctagtcct 
agtaataga gagagaatc actccagat aagttacca 
gacgagtta gggttaaaa gaaatgggt attgatgta 
cataattgg atcgacaaa gaaatggaa gatttagag 
cgcgagcaa agtgccata gatgaacaa gcggctgtt 
ttggaaaaa cagttacgt ctagttatg gaatctccg 
aatagcaat ggtgaggag gaggaagct ctaatggcc 
aagtggttt atgttcgtc aataagaaa aacgcttta 
cttaggagg caaatgcaa cttaacata ttggagaag 
gaggccgat ctagagacg aaatacaaa atgttgaat 
aaagaattg caaaagatc gcatcgata gaagactgg 
cggaaaacg gaggagcag agaaaccgg gagcaaatg 
ttgttggac gaacttgtg caaatagtt aataagcga 
gacgagctc gttcatcat ttggacaat caagaaaaa 
gcaatcgag gaagatgat ttaatagag caagattta 
tcacagttg gacttgcaa ccgaaggag aaatgtgta 
attcagtaa
    mRNA sequence
    • >TC003079 RA
      acaaataag aacaagttg tgaaagttg ggagcgacc 
gttgttacc gagtgagcc caaaactgt caatacagt 
gaaatgaca agagaaaaa taaattgat aatttacat 
cttttcgct ctgtttatc ttataagga aattagggg 
catattttg caacttttc cattttctc caattaatt 
tcaccatga actgctagt ggagtagac ttgaagtga 
gggttccag catgggctc tgtctggaa acggctcca 
gagagtgaa taaacgtgc ggcaaagtt tcagttcac 
ggtgtcata tcatcagct tgcagtaga attcacctc 
aaaatggca accccacaa actttcctt agtatggtc 
acgtaggtc tagaagggt ggcttccgc cccaatggc 
ttgggagcc aactcttca gaatgctta caagggatt 
agtggtttg gccggttcc agaaaatca tcaagtagc 
tattacttt atttagaga cccgagaac ttcagaatt 
agaagacaa ggaatggtc atttattat agaagatgt 
cccaacgac cggaaaaag acgccaact tgccgttgc 
acatgtcaa tatgcgaaa gtatgccag catcgaatc 
aactcactc agaattaca aatatcgtt taagactac 
aacaaagaa aattacttc cgccaaact tgactgcac 
tctatcgtg tgtcctttt acgcgaagg aaaagcgac 
agatgaaga catgcaatc gatggcgtc tctaatgtc 
tgtaaataa taacagcga cgatattgc cgcgttaga 
ggatgtaga agacgacga tttctctct atgtcagaa 
cgacaactc ggtcaaaag cagcattaa cgaagtgac 
ccaacattt acaacaaat gaccaacag cttgtcggg 
ctccgactt tgccagtac gccaattag cgttgcaag 
tattcagtc gttttcacg agacgataa aactccaac 
agctgagaa tttttcacc gattgggga aaaacccaa 
agctacgac tgttccacc acctagatt tagcgactt 
tgctgagga tgatgtcga tccgtattc ggaacaatg 
caccgaact gttagataa attagacgc cttggagga 
agacgaaga gagtgtgcc tagacctaa gagtgtaaa 
tttgaagtt aaaaccttt ggaactgaa ggaacatat 
tgacctgcc aaagaacca accccaaaa attgacgac 
tccagggca agatttatt agagtggtg taaggaaat 
gacgaaaaa ttatctcgg agttaaggt aacgaattt 
aacgacgag ctggaggaa cggaatggc cttttgtgc 
cgtcataca tcattttga accgagctt aattgactt 
caattctct ttcacctca cgatgtgaa aggaaattg 
taagatagc gtttgacgc cggtgataa gttgggtat 
tccgagggt gattgaacc gacagatat gcacatgtt 
ggcggtgcc tgataagtt ggcagtaat gacttactt 
gcaccaact gagggcaca ctttacggg tcaccagtt 
agaagtaca acaaatcgg taaaacttc cgaggaaag 
caattatgt cattggaaa attcaatac tgataaaga 
cacagatat tacgaagca agtcttcgg ccaagagat 
tataaattt aaggaaagc taagcagaa tcaacagtt 
gaataaatc gcaagattc gaaaaaaga gaaagagtt 
aaaccaaac caataagga aattagcca aaatcaaga 
catgaaaat tgccgcttc aaaactaca acttccatt 
aaaaatcac caatgaacc ggctgaaag tccgaaaga 
gcccaaaga aaagtcccc gactgttgt caaagacat 
gaaagacat tatcttgtt aggttctaa aagcataat 
gagtaaagt aatgtcttc gccttcaaa agacaaaat 
tcaggctaa gaaaactga agtgaagaa accgattct 
gatgacacg aagggagct cacggaccc gttcgggtc 
cgatgatga ggaggaagt tactgataa taaagtcat 
caataataa agtacctga agctgatga gatgaacgg 
ttctggtga tcataaagt taaggaaaa tcaaaacga 
tgtgttttc ggatcttcc caaaccgaa tgcgcaaat 
acttatgcg acattcaga gcaaagaga acgtgccag 
gcagttgct tgaacagac cagaaaaga cgcttccat 
caccacttc ccccactag gtctgaaga ggaaagaca 
agcgcaact gcgtgaacg tgcgagaaa aatactcgc 
agaagctcg gagagggtc caccccaca aactgagat 
tattcgact gagtccaga ctcggtcaa aaacttttt 
agaaaatga cgagaaaag tttccttga agggaatgc 
gaattttga acgacaaaa tagcaccgg ttctgaaaa 
gaacggtaa tgtttattc ggaagctaa ttcagaaaa 
gtctagtcc tagtaatag agagagaat cactccaga 
taagttacc agacgagtt agggttaaa agaaatggg 
tattgatgt acataattg gatcgacaa agaaatgga 
agatttaga gcgcgagca aagtgccat agatgaaca 
agcggctgt tttggaaaa acagttacg tctagttat 
ggaatctcc gaatagcaa tggtgagga ggaggaagc 
tctaatggc caagtggtt tatgttcgt caataagaa 
aaacgcttt acttaggag gcaaatgca acttaacat 
attggagaa ggaggccga tctagagac gaaatacaa 
aatgttgaa taaagaatt gcaaaagat cgcatcgat 
agaagactg gcggaaaac ggaggagca gagaaaccg 
ggagcaaat gttgttgga cgaacttgt gcaaatagt 
taataagcg agacgagct cgttcatca tttggacaa 
tcaagaaaa agcaatcga ggaagatga tttaataga 
gcaagattt atcacagtt ggacttgca accgaagga 
gaaatgtgt aattcagta aatcacttg gttagtttt 
ggcaaatta ttttgtaat tttcggcga aattgcttc 
aaattaatt acgatattg tatatgaaa tggtgcttt 
tatgtgata ttttattta gaattaacg agcaataga 
aataaagct tctctgatt taacgatac ttacaaaat 
aatttgttt aatctcgaa tacagttgt gtgtaaatt 
attaattta ttgccttaa tgaaccggt ttttaataa 
aaagacagt cttgttttt tgatatgat tcaaaaact 
acatgtacg agtatttaa gtcgtatta tttatttaa 
ttgtaaatg taaggaaca cattcatta tggattgga 
taatccgaa ttaaatgat ctaaatggg ctgtaaact 
acgccatcc acgtggtag tgcaatata ttaagggaa 
atttttctt tgctacttt ctattagaa ttgatcatg 
gcttggttg aaaagtcag tattttggt ggtcatgtc 
tatattttc gaacttggt tgtaacaat cgttggatg 
tgaatagat tacgctatg tatacattt gtacgtaat 
tacgtgatt gtgtttacc tatcgtcct accattagc 
ttaaaatta gcgttagtg gttgtaaaa taacttttt 
ttattgatg tatatttat ttgtaaggt aaccgatta 
agttaatgt aaataaact taagaagac tat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY