| General information | |||
| TC003079 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
scaffold_seqid14: 1394724..1403840 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC003079 | |
| General information | ||||
| iB_00512 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
- >TC003079 RA
MGSVWKRLQ RVNKRAAKF QFTVSYHQL AVEFTSKWQ PHKLSLVWS RRSRRVASA PMAWEPTLQ NAYKGLVVW PVPENHQVA ITLFRDPRT SELEDKEWS FIIEDVPTT GKRRQLAVA HVNMRKYAS IESTHSELQ ISFKTTTKK ITSAKLDCT LSCVLLREG KATDEDMQS MASLMSVNN NSDDIAALE DVEDDDFSL CQNDNSVKS SINEVTQHL QQMTNSLSG SDFASTPIS VASIQSFSR DDKTPTAEN FSPIGEKPK ATTVPPPRF SDFAEDDVD PYSEQCTEL LDKLDALEE DEESVPRPK SVNLKLKPL ELKEHIDLP KNQPQKLTT PGQDLLEWC KEMTKNYLG VKVTNLTTS WRNGMAFCA VIHHFEPSL IDFNSLSPH DVKGNCKIA FDAGDKLGI PRVIEPTDM HMLAVPDKL AVMTYLHQL RAHFTGHQL EVQQIGKTS EESNYVIGK FNTDKDTDI TKQVFGQEI INLRKAKQN QQLNKSQDS KKEKELNQT NKEISQNQD MKIAASKLQ LPLKITNEP AESPKEPKE KSPTVVKDM KDIILLGSK SIMSKVMSS PSKDKIQAK KTEVKKPIL MTRRELTDP FGSDDEEEV TDNKVINNK VPEADEMNG SGDHKVKEN QNDVFSDLP KPNAQILMR HSEQRERAR QLLEQTRKD ASITTSPTR SEEERQAQL RERARKILA EARRGSTPQ TEIIRLSPD SVKNFLEND EKSFLEGNA NFERQNSTG SEKNGNVYS EANSEKSSP SNRERITPD KLPDELGLK EMGIDVHNW IDKEMEDLE REQSAIDEQ AAVLEKQLR LVMESPNSN GEEEEALMA KWFMFVNKK NALLRRQMQ LNILEKEAD LETKYKMLN KELQKIASI EDWRKTEEQ RNREQMLLD ELVQIVNKR DELVHHLDN QEKAIEEDD LIEQDLSQL DLQPKEKCV IQ
- >TC003079 RA
atgggctct gtctggaaa cggctccag agagtgaat aaacgtgcg gcaaagttt cagttcacg gtgtcatat catcagctt gcagtagaa ttcacctca aaatggcaa ccccacaaa ctttcctta gtatggtca cgtaggtct agaagggtg gcttccgcc ccaatggct tgggagcca actcttcag aatgcttac aagggatta gtggtttgg ccggttcca gaaaatcat caagtagct attacttta tttagagac ccgagaact tcagaatta gaagacaag gaatggtca tttattata gaagatgtc ccaacgacc ggaaaaaga cgccaactt gccgttgca catgtcaat atgcgaaag tatgccagc atcgaatca actcactca gaattacaa atatcgttt aagactaca acaaagaaa attacttcc gccaaactt gactgcact ctatcgtgt gtcctttta cgcgaagga aaagcgaca gatgaagac atgcaatcg atggcgtct ctaatgtct gtaaataat aacagcgac gatattgcc gcgttagag gatgtagaa gacgacgat ttctctcta tgtcagaac gacaactcg gtcaaaagc agcattaac gaagtgacc caacattta caacaaatg accaacagc ttgtcgggc tccgacttt gccagtacg ccaattagc gttgcaagt attcagtcg ttttcacga gacgataaa actccaaca gctgagaat ttttcaccg attggggaa aaacccaaa gctacgact gttccacca cctagattt agcgacttt gctgaggat gatgtcgat ccgtattcg gaacaatgc accgaactg ttagataaa ttagacgcc ttggaggaa gacgaagag agtgtgcct agacctaag agtgtaaat ttgaagtta aaacctttg gaactgaag gaacatatt gacctgcca aagaaccaa ccccaaaaa ttgacgact ccagggcaa gatttatta gagtggtgt aaggaaatg acgaaaaat tatctcgga gttaaggta acgaattta acgacgagc tggaggaac ggaatggcc ttttgtgcc gtcatacat cattttgaa ccgagctta attgacttc aattctctt tcacctcac gatgtgaaa ggaaattgt aagatagcg tttgacgcc ggtgataag ttgggtatt ccgagggtg attgaaccg acagatatg cacatgttg gcggtgcct gataagttg gcagtaatg acttacttg caccaactg agggcacac tttacgggt caccagtta gaagtacaa caaatcggt aaaacttcc gaggaaagc aattatgtc attggaaaa ttcaatact gataaagac acagatatt acgaagcaa gtcttcggc caagagatt ataaattta aggaaagct aagcagaat caacagttg aataaatcg caagattcg aaaaaagag aaagagtta aaccaaacc aataaggaa attagccaa aatcaagac atgaaaatt gccgcttca aaactacaa cttccatta aaaatcacc aatgaaccg gctgaaagt ccgaaagag cccaaagaa aagtccccg actgttgtc aaagacatg aaagacatt atcttgtta ggttctaaa agcataatg agtaaagta atgtcttcg ccttcaaaa gacaaaatt caggctaag aaaactgaa gtgaagaaa ccgattctg atgacacga agggagctc acggacccg ttcgggtcc gatgatgag gaggaagtt actgataat aaagtcatc aataataaa gtacctgaa gctgatgag atgaacggt tctggtgat cataaagtt aaggaaaat caaaacgat gtgttttcg gatcttccc aaaccgaat gcgcaaata cttatgcga cattcagag caaagagaa cgtgccagg cagttgctt gaacagacc agaaaagac gcttccatc accacttcc cccactagg tctgaagag gaaagacaa gcgcaactg cgtgaacgt gcgagaaaa atactcgca gaagctcgg agagggtcc accccacaa actgagatt attcgactg agtccagac tcggtcaaa aacttttta gaaaatgac gagaaaagt ttccttgaa gggaatgcg aattttgaa cgacaaaat agcaccggt tctgaaaag aacggtaat gtttattcg gaagctaat tcagaaaag tctagtcct agtaataga gagagaatc actccagat aagttacca gacgagtta gggttaaaa gaaatgggt attgatgta cataattgg atcgacaaa gaaatggaa gatttagag cgcgagcaa agtgccata gatgaacaa gcggctgtt ttggaaaaa cagttacgt ctagttatg gaatctccg aatagcaat ggtgaggag gaggaagct ctaatggcc aagtggttt atgttcgtc aataagaaa aacgcttta cttaggagg caaatgcaa cttaacata ttggagaag gaggccgat ctagagacg aaatacaaa atgttgaat aaagaattg caaaagatc gcatcgata gaagactgg cggaaaacg gaggagcag agaaaccgg gagcaaatg ttgttggac gaacttgtg caaatagtt aataagcga gacgagctc gttcatcat ttggacaat caagaaaaa gcaatcgag gaagatgat ttaatagag caagattta tcacagttg gacttgcaa ccgaaggag aaatgtgta attcagtaa
- >TC003079 RA
acaaataag aacaagttg tgaaagttg ggagcgacc gttgttacc gagtgagcc caaaactgt caatacagt gaaatgaca agagaaaaa taaattgat aatttacat cttttcgct ctgtttatc ttataagga aattagggg catattttg caacttttc cattttctc caattaatt tcaccatga actgctagt ggagtagac ttgaagtga gggttccag catgggctc tgtctggaa acggctcca gagagtgaa taaacgtgc ggcaaagtt tcagttcac ggtgtcata tcatcagct tgcagtaga attcacctc aaaatggca accccacaa actttcctt agtatggtc acgtaggtc tagaagggt ggcttccgc cccaatggc ttgggagcc aactcttca gaatgctta caagggatt agtggtttg gccggttcc agaaaatca tcaagtagc tattacttt atttagaga cccgagaac ttcagaatt agaagacaa ggaatggtc atttattat agaagatgt cccaacgac cggaaaaag acgccaact tgccgttgc acatgtcaa tatgcgaaa gtatgccag catcgaatc aactcactc agaattaca aatatcgtt taagactac aacaaagaa aattacttc cgccaaact tgactgcac tctatcgtg tgtcctttt acgcgaagg aaaagcgac agatgaaga catgcaatc gatggcgtc tctaatgtc tgtaaataa taacagcga cgatattgc cgcgttaga ggatgtaga agacgacga tttctctct atgtcagaa cgacaactc ggtcaaaag cagcattaa cgaagtgac ccaacattt acaacaaat gaccaacag cttgtcggg ctccgactt tgccagtac gccaattag cgttgcaag tattcagtc gttttcacg agacgataa aactccaac agctgagaa tttttcacc gattgggga aaaacccaa agctacgac tgttccacc acctagatt tagcgactt tgctgagga tgatgtcga tccgtattc ggaacaatg caccgaact gttagataa attagacgc cttggagga agacgaaga gagtgtgcc tagacctaa gagtgtaaa tttgaagtt aaaaccttt ggaactgaa ggaacatat tgacctgcc aaagaacca accccaaaa attgacgac tccagggca agatttatt agagtggtg taaggaaat gacgaaaaa ttatctcgg agttaaggt aacgaattt aacgacgag ctggaggaa cggaatggc cttttgtgc cgtcataca tcattttga accgagctt aattgactt caattctct ttcacctca cgatgtgaa aggaaattg taagatagc gtttgacgc cggtgataa gttgggtat tccgagggt gattgaacc gacagatat gcacatgtt ggcggtgcc tgataagtt ggcagtaat gacttactt gcaccaact gagggcaca ctttacggg tcaccagtt agaagtaca acaaatcgg taaaacttc cgaggaaag caattatgt cattggaaa attcaatac tgataaaga cacagatat tacgaagca agtcttcgg ccaagagat tataaattt aaggaaagc taagcagaa tcaacagtt gaataaatc gcaagattc gaaaaaaga gaaagagtt aaaccaaac caataagga aattagcca aaatcaaga catgaaaat tgccgcttc aaaactaca acttccatt aaaaatcac caatgaacc ggctgaaag tccgaaaga gcccaaaga aaagtcccc gactgttgt caaagacat gaaagacat tatcttgtt aggttctaa aagcataat gagtaaagt aatgtcttc gccttcaaa agacaaaat tcaggctaa gaaaactga agtgaagaa accgattct gatgacacg aagggagct cacggaccc gttcgggtc cgatgatga ggaggaagt tactgataa taaagtcat caataataa agtacctga agctgatga gatgaacgg ttctggtga tcataaagt taaggaaaa tcaaaacga tgtgttttc ggatcttcc caaaccgaa tgcgcaaat acttatgcg acattcaga gcaaagaga acgtgccag gcagttgct tgaacagac cagaaaaga cgcttccat caccacttc ccccactag gtctgaaga ggaaagaca agcgcaact gcgtgaacg tgcgagaaa aatactcgc agaagctcg gagagggtc caccccaca aactgagat tattcgact gagtccaga ctcggtcaa aaacttttt agaaaatga cgagaaaag tttccttga agggaatgc gaattttga acgacaaaa tagcaccgg ttctgaaaa gaacggtaa tgtttattc ggaagctaa ttcagaaaa gtctagtcc tagtaatag agagagaat cactccaga taagttacc agacgagtt agggttaaa agaaatggg tattgatgt acataattg gatcgacaa agaaatgga agatttaga gcgcgagca aagtgccat agatgaaca agcggctgt tttggaaaa acagttacg tctagttat ggaatctcc gaatagcaa tggtgagga ggaggaagc tctaatggc caagtggtt tatgttcgt caataagaa aaacgcttt acttaggag gcaaatgca acttaacat attggagaa ggaggccga tctagagac gaaatacaa aatgttgaa taaagaatt gcaaaagat cgcatcgat agaagactg gcggaaaac ggaggagca gagaaaccg ggagcaaat gttgttgga cgaacttgt gcaaatagt taataagcg agacgagct cgttcatca tttggacaa tcaagaaaa agcaatcga ggaagatga tttaataga gcaagattt atcacagtt ggacttgca accgaagga gaaatgtgt aattcagta aatcacttg gttagtttt ggcaaatta ttttgtaat tttcggcga aattgcttc aaattaatt acgatattg tatatgaaa tggtgcttt tatgtgata ttttattta gaattaacg agcaataga aataaagct tctctgatt taacgatac ttacaaaat aatttgttt aatctcgaa tacagttgt gtgtaaatt attaattta ttgccttaa tgaaccggt ttttaataa aaagacagt cttgttttt tgatatgat tcaaaaact acatgtacg agtatttaa gtcgtatta tttatttaa ttgtaaatg taaggaaca cattcatta tggattgga taatccgaa ttaaatgat ctaaatggg ctgtaaact acgccatcc acgtggtag tgcaatata ttaagggaa atttttctt tgctacttt ctattagaa ttgatcatg gcttggttg aaaagtcag tattttggt ggtcatgtc tatattttc gaacttggt tgtaacaat cgttggatg tgaatagat tacgctatg tatacattt gtacgtaat tacgtgatt gtgtttacc tatcgtcct accattagc ttaaaatta gcgttagtg gttgtaaaa taacttttt ttattgatg tatatttat ttgtaaggt aaccgatta agttaatgt aaataaact taagaagac tat