Status
Please wait ...
Gene information
General information
TC003079 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 2473235..2482351
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003079
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00512

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 9 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003079 RA
      MGSVWKRLQ RVNKRAAKF QFTVSYHQL AVEFTSKWQ 
      PHKLSLVWS RRSRRVASA PMAWEPTLQ NAYKGLVVW 
      PVPENHQVA ITLFRDPRT SELEDKEWS FIIEDVPTT 
      GKRRQLAVA HVNMRKYAS IESTHSELQ ISFKTTTKK 
      ITSAKLDCT LSCVLLREG KATDEDMQS MASLMSVNN 
      NSDDIAALE DVEDDDFSL CQNDNSVKS SINEVTQHL 
      QQMTNSLSG SDFASTPIS VASIQSFSR DDKTPTAEN 
      FSPIGEKPK ATTVPPPRF SDFAEDDVD PYSEQCTEL 
      LDKLDALEE DEESVPRPK SVNLKLKPL ELKEHIDLP 
      KNQPQKLTT PGQDLLEWC KEMTKNYLG VKVTNLTTS 
      WRNGMAFCA VIHHFEPSL IDFNSLSPH DVKGNCKIA 
      FDAGDKLGI PRVIEPTDM HMLAVPDKL AVMTYLHQL 
      RAHFTGHQL EVQQIGKTS EESNYVIGK FNTDKDTDI 
      TKQVFGQEI INLRKAKQN QQLNKSQDS KKEKELNQT 
      NKEISQNQD MKIAASKLQ LPLKITNEP AESPKEPKE 
      KSPTVVKDM KDIILLGSK SIMSKVMSS PSKDKIQAK 
      KTEVKKPIL MTRRELTDP FGSDDEEEV TDNKVINNK 
      VPEADEMNG SGDHKVKEN QNDVFSDLP KPNAQILMR 
      HSEQRERAR QLLEQTRKD ASITTSPTR SEEERQAQL 
      RERARKILA EARRGSTPQ TEIIRLSPD SVKNFLEND 
      EKSFLEGNA NFERQNSTG SEKNGNVYS EANSEKSSP 
      SNRERITPD KLPDELGLK EMGIDVHNW IDKEMEDLE 
      REQSAIDEQ AAVLEKQLR LVMESPNSN GEEEEALMA 
      KWFMFVNKK NALLRRQMQ LNILEKEAD LETKYKMLN 
      KELQKIASI EDWRKTEEQ RNREQMLLD ELVQIVNKR 
      DELVHHLDN QEKAIEEDD LIEQDLSQL DLQPKEKCV 
      IQ
    CDS sequence
    • >TC003079 RA
      atgggctct gtctggaaa cggctccag agagtgaat 
      aaacgtgcg gcaaagttt cagttcacg gtgtcatat 
      catcagctt gcagtagaa ttcacctca aaatggcaa 
      ccccacaaa ctttcctta gtatggtca cgtaggtct 
      agaagggtg gcttccgcc ccaatggct tgggagcca 
      actcttcag aatgcttac aagggatta gtggtttgg 
      ccggttcca gaaaatcat caagtagct attacttta 
      tttagagac ccgagaact tcagaatta gaagacaag 
      gaatggtca tttattata gaagatgtc ccaacgacc 
      ggaaaaaga cgccaactt gccgttgca catgtcaat 
      atgcgaaag tatgccagc atcgaatca actcactca 
      gaattacaa atatcgttt aagactaca acaaagaaa 
      attacttcc gccaaactt gactgcact ctatcgtgt 
      gtcctttta cgcgaagga aaagcgaca gatgaagac 
      atgcaatcg atggcgtct ctaatgtct gtaaataat 
      aacagcgac gatattgcc gcgttagag gatgtagaa 
      gacgacgat ttctctcta tgtcagaac gacaactcg 
      gtcaaaagc agcattaac gaagtgacc caacattta 
      caacaaatg accaacagc ttgtcgggc tccgacttt 
      gccagtacg ccaattagc gttgcaagt attcagtcg 
      ttttcacga gacgataaa actccaaca gctgagaat 
      ttttcaccg attggggaa aaacccaaa gctacgact 
      gttccacca cctagattt agcgacttt gctgaggat 
      gatgtcgat ccgtattcg gaacaatgc accgaactg 
      ttagataaa ttagacgcc ttggaggaa gacgaagag 
      agtgtgcct agacctaag agtgtaaat ttgaagtta 
      aaacctttg gaactgaag gaacatatt gacctgcca 
      aagaaccaa ccccaaaaa ttgacgact ccagggcaa 
      gatttatta gagtggtgt aaggaaatg acgaaaaat 
      tatctcgga gttaaggta acgaattta acgacgagc 
      tggaggaac ggaatggcc ttttgtgcc gtcatacat 
      cattttgaa ccgagctta attgacttc aattctctt 
      tcacctcac gatgtgaaa ggaaattgt aagatagcg 
      tttgacgcc ggtgataag ttgggtatt ccgagggtg 
      attgaaccg acagatatg cacatgttg gcggtgcct 
      gataagttg gcagtaatg acttacttg caccaactg 
      agggcacac tttacgggt caccagtta gaagtacaa 
      caaatcggt aaaacttcc gaggaaagc aattatgtc 
      attggaaaa ttcaatact gataaagac acagatatt 
      acgaagcaa gtcttcggc caagagatt ataaattta 
      aggaaagct aagcagaat caacagttg aataaatcg 
      caagattcg aaaaaagag aaagagtta aaccaaacc 
      aataaggaa attagccaa aatcaagac atgaaaatt 
      gccgcttca aaactacaa cttccatta aaaatcacc 
      aatgaaccg gctgaaagt ccgaaagag cccaaagaa 
      aagtccccg actgttgtc aaagacatg aaagacatt 
      atcttgtta ggttctaaa agcataatg agtaaagta 
      atgtcttcg ccttcaaaa gacaaaatt caggctaag 
      aaaactgaa gtgaagaaa ccgattctg atgacacga 
      agggagctc acggacccg ttcgggtcc gatgatgag 
      gaggaagtt actgataat aaagtcatc aataataaa 
      gtacctgaa gctgatgag atgaacggt tctggtgat 
      cataaagtt aaggaaaat caaaacgat gtgttttcg 
      gatcttccc aaaccgaat gcgcaaata cttatgcga 
      cattcagag caaagagaa cgtgccagg cagttgctt 
      gaacagacc agaaaagac gcttccatc accacttcc 
      cccactagg tctgaagag gaaagacaa gcgcaactg 
      cgtgaacgt gcgagaaaa atactcgca gaagctcgg 
      agagggtcc accccacaa actgagatt attcgactg 
      agtccagac tcggtcaaa aacttttta gaaaatgac 
      gagaaaagt ttccttgaa gggaatgcg aattttgaa 
      cgacaaaat agcaccggt tctgaaaag aacggtaat 
      gtttattcg gaagctaat tcagaaaag tctagtcct 
      agtaataga gagagaatc actccagat aagttacca 
      gacgagtta gggttaaaa gaaatgggt attgatgta 
      cataattgg atcgacaaa gaaatggaa gatttagag 
      cgcgagcaa agtgccata gatgaacaa gcggctgtt 
      ttggaaaaa cagttacgt ctagttatg gaatctccg 
      aatagcaat ggtgaggag gaggaagct ctaatggcc 
      aagtggttt atgttcgtc aataagaaa aacgcttta 
      cttaggagg caaatgcaa cttaacata ttggagaag 
      gaggccgat ctagagacg aaatacaaa atgttgaat 
      aaagaattg caaaagatc gcatcgata gaagactgg 
      cggaaaacg gaggagcag agaaaccgg gagcaaatg 
      ttgttggac gaacttgtg caaatagtt aataagcga 
      gacgagctc gttcatcat ttggacaat caagaaaaa 
      gcaatcgag gaagatgat ttaatagag caagattta 
      tcacagttg gacttgcaa ccgaaggag aaatgtgta 
      attcagtaa
    mRNA sequence
    • >TC003079 RA
      acaaataag aacaagttg tgaaagttg ggagcgacc 
      gttgttacc gagtgagcc caaaactgt caatacagt 
      gaaatgaca agagaaaaa taaattgat aatttacat 
      cttttcgct ctgtttatc ttataagga aattagggg 
      catattttg caacttttc cattttctc caattaatt 
      tcaccatga actgctagt ggagtagac ttgaagtga 
      gggttccag catgggctc tgtctggaa acggctcca 
      gagagtgaa taaacgtgc ggcaaagtt tcagttcac 
      ggtgtcata tcatcagct tgcagtaga attcacctc 
      aaaatggca accccacaa actttcctt agtatggtc 
      acgtaggtc tagaagggt ggcttccgc cccaatggc 
      ttgggagcc aactcttca gaatgctta caagggatt 
      agtggtttg gccggttcc agaaaatca tcaagtagc 
      tattacttt atttagaga cccgagaac ttcagaatt 
      agaagacaa ggaatggtc atttattat agaagatgt 
      cccaacgac cggaaaaag acgccaact tgccgttgc 
      acatgtcaa tatgcgaaa gtatgccag catcgaatc 
      aactcactc agaattaca aatatcgtt taagactac 
      aacaaagaa aattacttc cgccaaact tgactgcac 
      tctatcgtg tgtcctttt acgcgaagg aaaagcgac 
      agatgaaga catgcaatc gatggcgtc tctaatgtc 
      tgtaaataa taacagcga cgatattgc cgcgttaga 
      ggatgtaga agacgacga tttctctct atgtcagaa 
      cgacaactc ggtcaaaag cagcattaa cgaagtgac 
      ccaacattt acaacaaat gaccaacag cttgtcggg 
      ctccgactt tgccagtac gccaattag cgttgcaag 
      tattcagtc gttttcacg agacgataa aactccaac 
      agctgagaa tttttcacc gattgggga aaaacccaa 
      agctacgac tgttccacc acctagatt tagcgactt 
      tgctgagga tgatgtcga tccgtattc ggaacaatg 
      caccgaact gttagataa attagacgc cttggagga 
      agacgaaga gagtgtgcc tagacctaa gagtgtaaa 
      tttgaagtt aaaaccttt ggaactgaa ggaacatat 
      tgacctgcc aaagaacca accccaaaa attgacgac 
      tccagggca agatttatt agagtggtg taaggaaat 
      gacgaaaaa ttatctcgg agttaaggt aacgaattt 
      aacgacgag ctggaggaa cggaatggc cttttgtgc 
      cgtcataca tcattttga accgagctt aattgactt 
      caattctct ttcacctca cgatgtgaa aggaaattg 
      taagatagc gtttgacgc cggtgataa gttgggtat 
      tccgagggt gattgaacc gacagatat gcacatgtt 
      ggcggtgcc tgataagtt ggcagtaat gacttactt 
      gcaccaact gagggcaca ctttacggg tcaccagtt 
      agaagtaca acaaatcgg taaaacttc cgaggaaag 
      caattatgt cattggaaa attcaatac tgataaaga 
      cacagatat tacgaagca agtcttcgg ccaagagat 
      tataaattt aaggaaagc taagcagaa tcaacagtt 
      gaataaatc gcaagattc gaaaaaaga gaaagagtt 
      aaaccaaac caataagga aattagcca aaatcaaga 
      catgaaaat tgccgcttc aaaactaca acttccatt 
      aaaaatcac caatgaacc ggctgaaag tccgaaaga 
      gcccaaaga aaagtcccc gactgttgt caaagacat 
      gaaagacat tatcttgtt aggttctaa aagcataat 
      gagtaaagt aatgtcttc gccttcaaa agacaaaat 
      tcaggctaa gaaaactga agtgaagaa accgattct 
      gatgacacg aagggagct cacggaccc gttcgggtc 
      cgatgatga ggaggaagt tactgataa taaagtcat 
      caataataa agtacctga agctgatga gatgaacgg 
      ttctggtga tcataaagt taaggaaaa tcaaaacga 
      tgtgttttc ggatcttcc caaaccgaa tgcgcaaat 
      acttatgcg acattcaga gcaaagaga acgtgccag 
      gcagttgct tgaacagac cagaaaaga cgcttccat 
      caccacttc ccccactag gtctgaaga ggaaagaca 
      agcgcaact gcgtgaacg tgcgagaaa aatactcgc 
      agaagctcg gagagggtc caccccaca aactgagat 
      tattcgact gagtccaga ctcggtcaa aaacttttt 
      agaaaatga cgagaaaag tttccttga agggaatgc 
      gaattttga acgacaaaa tagcaccgg ttctgaaaa 
      gaacggtaa tgtttattc ggaagctaa ttcagaaaa 
      gtctagtcc tagtaatag agagagaat cactccaga 
      taagttacc agacgagtt agggttaaa agaaatggg 
      tattgatgt acataattg gatcgacaa agaaatgga 
      agatttaga gcgcgagca aagtgccat agatgaaca 
      agcggctgt tttggaaaa acagttacg tctagttat 
      ggaatctcc gaatagcaa tggtgagga ggaggaagc 
      tctaatggc caagtggtt tatgttcgt caataagaa 
      aaacgcttt acttaggag gcaaatgca acttaacat 
      attggagaa ggaggccga tctagagac gaaatacaa 
      aatgttgaa taaagaatt gcaaaagat cgcatcgat 
      agaagactg gcggaaaac ggaggagca gagaaaccg 
      ggagcaaat gttgttgga cgaacttgt gcaaatagt 
      taataagcg agacgagct cgttcatca tttggacaa 
      tcaagaaaa agcaatcga ggaagatga tttaataga 
      gcaagattt atcacagtt ggacttgca accgaagga 
      gaaatgtgt aattcagta aatcacttg gttagtttt 
      ggcaaatta ttttgtaat tttcggcga aattgcttc 
      aaattaatt acgatattg tatatgaaa tggtgcttt 
      tatgtgata ttttattta gaattaacg agcaataga 
      aataaagct tctctgatt taacgatac ttacaaaat 
      aatttgttt aatctcgaa tacagttgt gtgtaaatt 
      attaattta ttgccttaa tgaaccggt ttttaataa 
      aaagacagt cttgttttt tgatatgat tcaaaaact 
      acatgtacg agtatttaa gtcgtatta tttatttaa 
      ttgtaaatg taaggaaca cattcatta tggattgga 
      taatccgaa ttaaatgat ctaaatggg ctgtaaact 
      acgccatcc acgtggtag tgcaatata ttaagggaa 
      atttttctt tgctacttt ctattagaa ttgatcatg 
      gcttggttg aaaagtcag tattttggt ggtcatgtc 
      tatattttc gaacttggt tgtaacaat cgttggatg 
      tgaatagat tacgctatg tatacattt gtacgtaat 
      tacgtgatt gtgtttacc tatcgtcct accattagc 
      ttaaaatta gcgttagtg gttgtaaaa taacttttt 
      ttattgatg tatatttat ttgtaaggt aaccgatta 
      agttaatgt aaataaact taagaagac tat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY