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Gene information
General information
TC003078 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 2625519..2636644
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC003078
    iBeetle screen iB_00511

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    remarks: animals lost,
    Adult morphology
    remarks: animals lost,
    Fertility
    vitellarium mostly not present - Number of animals showing the phenotype: 4

    Analysis of larval stage 1 cuticle
    remarks: animals lost,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 90.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 9 (11 dpi)
    Days post injection

    eclosion delayed (still prepupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003078 RA
      MNENQNAKN EETPLEIFE TVTALVNEI VNSSPVSPQ 
SVSRTSAPS TSAPTKATE QASTAQLPQ VYSRQANLL 
PTYEIFRTS RQFDESQSD VAVEQYLAR LRAAKTSRP 
PTPSPPCVP PSYSTVIKQ GRPSLDIRR DVRSMEMQH 
ISPFSRIPP PSYNQIHGV WSREISNNS SEEVLLGPD 
PIYIWCSHC ATVVITATQ AQRSKFTHI TAAALCLFG 
CWPCAMLPY CMNSCKNIY HYCPDCNYL FGIKNCPKT 
LTILNMSRL VVKNLPKNI TEEKLRNLF QEKGTITDV 
QLKYTPEGK FREFAFVGY QNENEAQEA IKHFDGMFM 
HTRKIKVEP CALLGDSKK PKAWSKYAP ESTAYKKAH 
GILTKEEDN DKPKVAKKN QKEDETIEL LKKYQDDPM 
FEEFLELHN PEESKKLRK IIEKQKEHD VKSESEEDE 
GKGSSSEND SDEETQDRL ADQKISDLE YMKKLVKKK 
EKPKKEPKK LDLFTLKLR GLPYNSRKK DIKQFLKPV 
TPFSIRLPA KIHGIAYVG FKTEKDYKK ALLKDRSFI 
AGKRISVME YSGKNNTEH EETSNNVRK KWVSQEEAL 
KNEEDIAET GKIFVRNLA YTTTEDDVE NLFSKFGPL 
AEVNLPVDP TSKKIKGFG TVTFVMPEH AARAYGELD 
GSILHGRMV HLLPGKSKD VADEPDLEE STNFKKKKE 
AKQKSEAGS SHNWNSLFL GHNAVAEVI ADTYSTSKE 
KVMGLHDDG SAAVRLALG ETQIVAQTR KYLEQEGVQ 
LDAFSNNKC KRSKTVILV KNLPNKTEA KEIRTIFEK 
FGLVGRLIL PPSGITAIV EFLEPSEAR KAFTCLAYT 
KFKNVPLYL EWAPENSLG ERKEPPVTN EDTTEVKQS 
ENNNEVEET VEESEPDTT LFVKNLNFQ TTDDGLRKH 
FESCGKLAY ASVATKKDK NDPSKRLSM GYGFVRFIH 
KSSADKALK TLQQSTLDG KSLELKRSE RTLNNEVKV 
VKKSTKATK QTGTKILVR NIPFQANQK EIRELFSVF 
GEIKALRLP KKMALDGGS HRGFAFVDF VAASDAKNA 
FEALCQSTH LYGRRLVLE WASSEDSVD DIRKRTAAH 
FQTTEAVKS KKSVLSID
    CDS sequence
    • >TC003078 RA
      atgaacgaa aaccaaaat gcaaaaaac gaagaaact 
cccctcgaa attttcgaa acagtaaca gctctagta 
aatgaaata gtcaactca agtcctgta agtccacaa 
agtgtatct agaactagc gcaccatca acgagtgct 
ccaacaaaa gcaaccgaa caagcctcc acagcccaa 
ctcccgcaa gtctattct aggcaagca aaccttctc 
ccaacttac gaaatattc agaacttcg cgacaattc 
gacgaatcg caaagcgat gtcgctgtt gagcaatac 
ttggcccgc cttcgggct gcaaagaca agcagaccg 
ccaacgccg tcgcccccc tgtgtgccc cccagctac 
tccacggta ataaagcag gggcgcccc tcgctagat 
ataaggagg gatgtgagg tcaatggaa atgcagcac 
attagcccc ttttcgaga atcccgcca ccgtcttat 
aaccaaata catggtgtg tggtctcgg gaaattagc 
aacaattct tctgaggaa gtgttgcta gggccagac 
cctatatac atttggtgc tcacactgt gccactgtt 
gttattaca gcgacacag gctcagcgt tcgaagttt 
acacatatt acagctgcg gccttgtgt ctttttggt 
tgttggcct tgcgcgatg ttaccctac tgcatgaat 
tcatgcaag aatatttac cattattgc ccagattgc 
aattacttg tttgggata aaaaattgt ccaaaaaca 
ttgacaata ttgaacatg tctcgtttg gtcgttaaa 
aatttacct aaaaatata actgaagaa aaactcaga 
aatttgttc caagagaaa ggcactata actgatgtt 
cagttaaag tacacccct gagggtaaa tttcgtgag 
tttgctttc gtgggctac cagaatgaa aatgaagcc 
caggaagct attaaacac tttgatggc atgttcatg 
cacaccaga aagataaag gttgagcca tgtgcacta 
ttaggggat tccaagaaa cctaaagct tggagtaaa 
tatgcccca gaaagtacc gcatataag aaggctcac 
ggtatttta acaaaggaa gaagacaat gataaaccc 
aaagttgca aagaagaat caaaaagaa gatgaaact 
atagaacta cttaaaaaa tatcaagat gatccaatg 
tttgaagaa tttttagag cttcacaat cctgaagaa 
agcaagaaa ttgcgcaaa attatagaa aaacaaaaa 
gaacacgat gtcaaatca gaaagtgag gaagatgag 
ggaaagggt agctcttca gaaaatgac tcagatgaa 
gaaacgcaa gacagatta gctgaccaa aaaatcagt 
gacttagaa tacatgaaa aaactggta aagaagaaa 
gaaaaaccg aagaaggaa cccaaaaag ttggattta 
tttacactg aagttgaga ggccttcca tataattcc 
agaaagaaa gatataaaa caattccta aaacccgtt 
actcccttt tctatcaga cttcctgca aaaattcac 
gggattgcg tatgtgggc tttaagacc gaaaaagac 
tacaagaaa gccctcctg aaagacaga agtttcatc 
gccgggaaa agaatttcc gtcatggaa tactccgga 
aaaaataac accgaacac gaggaaact tcaaataat 
gttcggaaa aaatgggtg agtcaggag gaggctctc 
aaaaacgag gaagatatc gcggagacg ggcaaaatt 
tttgtgaga aatttggca tacactaca acagaagac 
gatgttgag aatctattt tcaaaattt ggcccgcta 
gctgaggtg aatttgccg gtggatccc acgtctaaa 
aaaatcaaa ggctttggc actgtgaca tttgtgatg 
cctgagcac gcggctaga gcttacggg gaactggat 
ggctctatt ctgcacggg cgcatggtg caccttctg 
ccaggaaaa agcaaagat gtggccgat gaaccagac 
ttagaagaa agtacgaat tttaagaag aagaaggaa 
gcgaaacag aaatctgaa gctgggtcg tctcataac 
tggaattcg ctatttttg gggcacaat gcagttgct 
gaagtcatt gcagacact tacagtact tcaaaggaa 
aaagttatg gggttacat gatgacggt agcgctgct 
gtgagactt gcactggga gagacacaa atcgtggca 
caaactagg aaatatctg gagcaagaa ggcgtccaa 
cttgatgct ttcagtaac aataaatgt aaaaggtcg 
aaaacagtg atccttgta aagaatttg ccgaataaa 
actgaagcc aaggagatt agaacaata tttgaaaag 
tttggcttg gttggacgg ttgattttg ccccctagt 
ggcattaca gcaattgtt gaatttctt gaaccgtct 
gaggcacgg aaagctttc acttgtttg gcttacacg 
aagtttaaa aatgtgcca ctttattta gagtgggct 
ccggaaaat tctctaggt gaacgaaaa gagccacct 
gtaacgaat gaagacact acagaagtt aaacaaagt 
gaaaataat aatgaagtt gaagaaact gtggaagaa 
tctgaacct gatactact ttatttgtt aaaaacttg 
aatttccaa acgactgat gacggtcta aggaagcat 
tttgaaagt tgtggcaaa cttgcgtac gccagcgtt 
gcaactaaa aaagacaaa aacgaccca agtaaacgt 
ctatccatg ggatatggc tttgttagg tttatacat 
aaaagcagc gctgataag gctctcaaa acccttcag 
cagagcact ctagacggc aaatcccta gaattgaag 
agatctgaa cggacgttg aacaacgag gtcaaagtt 
gttaaaaaa agcaccaaa gcaacgaaa caaacagga 
accaagatt ttggttcga aatatccca ttccaggct 
aaccagaag gaaattagg gaacttttt agtgttttt 
ggcgaaatt aaagcgtta aggctccca aagaaaatg 
gccttggat gggggctca catcgaggt tttgcgttt 
gtcgatttt gtggcagcc tctgatgcc aagaatgca 
tttgaggct ttgtgtcaa agtacccac ttgtacggt 
cggagacta gttctcgag tgggcctca agtgaggac 
agtgtggac gatatcagg aagcgaaca gcggctcac 
ttccaaacc actgaagca gtcaagtcg aagaaaagt 
gttttaagc attgattaa
    mRNA sequence
    • >TC003078 RA
      aaccccgaa tccaaaata tgtccaagt aatgaacga 
aaaccaaaa tgcaaaaaa cgaagaaac tcccctcga 
aattttcga aacagtaac agctctagt aaatgaaat 
agtcaactc aagtcctgt aagtccaca aagtgtatc 
tagaactag cgcaccatc aacgagtgc tccaacaaa 
agcaaccga acaagcctc cacagccca actcccgca 
agtctattc taggcaagc aaaccttct cccaactta 
cgaaatatt cagaacttc gcgacaatt cgacgaatc 
gcaaagcga tgtcgctgt tgagcaata cttggcccg 
ccttcgggc tgcaaagac aagcagacc gccaacgcc 
gtcgccccc ctgtgtgcc ccccagcta ctccacggt 
aataaagca ggggcgccc ctcgctaga tataaggag 
ggatgtgag gtcaatgga aatgcagca cattagccc 
cttttcgag aatcccgcc accgtctta taaccaaat 
acatggtgt gtggtctcg ggaaattag caacaattc 
ttctgagga agtgttgct agggccaga ccctatata 
catttggtg ctcacactg tgccactgt tgttattac 
agcgacaca ggctcagcg ttcgaagtt tacacatat 
tacagctgc ggccttgtg tctttttgg ttgttggcc 
ttgcgcgat gttacccta ctgcatgaa ttcatgcaa 
gaatattta ccattattg cccagattg caattactt 
gtttgggat aaaaaattg tccaaaaac attgacaat 
attgaacat gtctcgttt ggtcgttaa aaatttacc 
taaaaatat aactgaaga aaaactcag aaatttgtt 
ccaagagaa aggcactat aactgatgt tcagttaaa 
gtacacccc tgagggtaa atttcgtga gtttgcttt 
cgtgggcta ccagaatga aaatgaagc ccaggaagc 
tattaaaca ctttgatgg catgttcat gcacaccag 
aaagataaa ggttgagcc atgtgcact attagggga 
ttccaagaa acctaaagc ttggagtaa atatgcccc 
agaaagtac cgcatataa gaaggctca cggtatttt 
aacaaagga agaagacaa tgataaacc caaagttgc 
aaagaagaa tcaaaaaga agatgaaac tatagaact 
acttaaaaa atatcaaga tgatccaat gtttgaaga 
atttttaga gcttcacaa tcctgaaga aagcaagaa 
attgcgcaa aattataga aaaacaaaa agaacacga 
tgtcaaatc agaaagtga ggaagatga gggaaaggg 
tagctcttc agaaaatga ctcagatga agaaacgca 
agacagatt agctgacca aaaaatcag tgacttaga 
atacatgaa aaaactggt aaagaagaa agaaaaacc 
gaagaagga acccaaaaa gttggattt atttacact 
gaagttgag aggccttcc atataattc cagaaagaa 
agatataaa acaattcct aaaacccgt tactccctt 
ttctatcag acttcctgc aaaaattca cgggattgc 
gtatgtggg ctttaagac cgaaaaaga ctacaagaa 
agccctcct gaaagacag aagtttcat cgccgggaa 
aagaatttc cgtcatgga atactccgg aaaaaataa 
caccgaaca cgaggaaac ttcaaataa tgttcggaa 
aaaatgggt gagtcagga ggaggctct caaaaacga 
ggaagatat cgcggagac gggcaaaat ttttgtgag 
aaatttggc atacactac aacagaaga cgatgttga 
gaatctatt ttcaaaatt tggcccgct agctgaggt 
gaatttgcc ggtggatcc cacgtctaa aaaaatcaa 
aggctttgg cactgtgac atttgtgat gcctgagca 
cgcggctag agcttacgg ggaactgga tggctctat 
tctgcacgg gcgcatggt gcaccttct gccaggaaa 
aagcaaaga tgtggccga tgaaccaga cttagaaga 
aagtacgaa ttttaagaa gaagaagga agcgaaaca 
gaaatctga agctgggtc gtctcataa ctggaattc 
gctattttt ggggcacaa tgcagttgc tgaagtcat 
tgcagacac ttacagtac ttcaaagga aaaagttat 
ggggttaca tgatgacgg tagcgctgc tgtgagact 
tgcactggg agagacaca aatcgtggc acaaactag 
gaaatatct ggagcaaga aggcgtcca acttgatgc 
tttcagtaa caataaatg taaaaggtc gaaaacagt 
gatccttgt aaagaattt gccgaataa aactgaagc 
caaggagat tagaacaat atttgaaaa gtttggctt 
ggttggacg gttgatttt gccccctag tggcattac 
agcaattgt tgaatttct tgaaccgtc tgaggcacg 
gaaagcttt cacttgttt ggcttacac gaagtttaa 
aaatgtgcc actttattt agagtgggc tccggaaaa 
ttctctagg tgaacgaaa agagccacc tgtaacgaa 
tgaagacac tacagaagt taaacaaag tgaaaataa 
taatgaagt tgaagaaac tgtggaaga atctgaacc 
tgatactac tttatttgt taaaaactt gaatttcca 
aacgactga tgacggtct aaggaagca ttttgaaag 
ttgtggcaa acttgcgta cgccagcgt tgcaactaa 
aaaagacaa aaacgaccc aagtaaacg tctatccat 
gggatatgg ctttgttag gtttataca taaaagcag 
cgctgataa ggctctcaa aacccttca gcagagcac 
tctagacgg caaatccct agaattgaa gagatctga 
acggacgtt gaacaacga ggtcaaagt tgttaaaaa 
aagcaccaa agcaacgaa acaaacagg aaccaagat 
tttggttcg aaatatccc attccaggc taaccagaa 
ggaaattag ggaactttt tagtgtttt tggcgaaat 
taaagcgtt aaggctccc aaagaaaat ggccttgga 
tgggggctc acatcgagg ttttgcgtt tgtcgattt 
tgtggcagc ctctgatgc caagaatgc atttgaggc 
tttgtgtca aagtaccca cttgtacgg tcggagact 
agttctcga gtgggcctc aagtgagga cagtgtgga 
cgatatcag gaagcgaac agcggctca cttccaaac 
cactgaagc agtcaagtc gaagaaaag tgttttaag 
cattgatta attaaaaat aaaaactac actttgtct 
agtcaagtt tattacctc tagaataaa ccaacatta 
caaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY