Status
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Gene information
General information
TC003078 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 2625519..2636644
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003078
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00511

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    remarks: animals lost,
    Adult morphology
    remarks: animals lost,
    Fertility
    vitellarium mostly not present - Number of animals showing the phenotype: 4

    Analysis of larval stage 1 cuticle
    remarks: animals lost,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 90.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 9 (11 dpi)
    Days post injection


    eclosion delayed (still prepupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003078 RA
      MNENQNAKN EETPLEIFE TVTALVNEI VNSSPVSPQ 
      SVSRTSAPS TSAPTKATE QASTAQLPQ VYSRQANLL 
      PTYEIFRTS RQFDESQSD VAVEQYLAR LRAAKTSRP 
      PTPSPPCVP PSYSTVIKQ GRPSLDIRR DVRSMEMQH 
      ISPFSRIPP PSYNQIHGV WSREISNNS SEEVLLGPD 
      PIYIWCSHC ATVVITATQ AQRSKFTHI TAAALCLFG 
      CWPCAMLPY CMNSCKNIY HYCPDCNYL FGIKNCPKT 
      LTILNMSRL VVKNLPKNI TEEKLRNLF QEKGTITDV 
      QLKYTPEGK FREFAFVGY QNENEAQEA IKHFDGMFM 
      HTRKIKVEP CALLGDSKK PKAWSKYAP ESTAYKKAH 
      GILTKEEDN DKPKVAKKN QKEDETIEL LKKYQDDPM 
      FEEFLELHN PEESKKLRK IIEKQKEHD VKSESEEDE 
      GKGSSSEND SDEETQDRL ADQKISDLE YMKKLVKKK 
      EKPKKEPKK LDLFTLKLR GLPYNSRKK DIKQFLKPV 
      TPFSIRLPA KIHGIAYVG FKTEKDYKK ALLKDRSFI 
      AGKRISVME YSGKNNTEH EETSNNVRK KWVSQEEAL 
      KNEEDIAET GKIFVRNLA YTTTEDDVE NLFSKFGPL 
      AEVNLPVDP TSKKIKGFG TVTFVMPEH AARAYGELD 
      GSILHGRMV HLLPGKSKD VADEPDLEE STNFKKKKE 
      AKQKSEAGS SHNWNSLFL GHNAVAEVI ADTYSTSKE 
      KVMGLHDDG SAAVRLALG ETQIVAQTR KYLEQEGVQ 
      LDAFSNNKC KRSKTVILV KNLPNKTEA KEIRTIFEK 
      FGLVGRLIL PPSGITAIV EFLEPSEAR KAFTCLAYT 
      KFKNVPLYL EWAPENSLG ERKEPPVTN EDTTEVKQS 
      ENNNEVEET VEESEPDTT LFVKNLNFQ TTDDGLRKH 
      FESCGKLAY ASVATKKDK NDPSKRLSM GYGFVRFIH 
      KSSADKALK TLQQSTLDG KSLELKRSE RTLNNEVKV 
      VKKSTKATK QTGTKILVR NIPFQANQK EIRELFSVF 
      GEIKALRLP KKMALDGGS HRGFAFVDF VAASDAKNA 
      FEALCQSTH LYGRRLVLE WASSEDSVD DIRKRTAAH 
      FQTTEAVKS KKSVLSID
    CDS sequence
    • >TC003078 RA
      atgaacgaa aaccaaaat gcaaaaaac gaagaaact 
      cccctcgaa attttcgaa acagtaaca gctctagta 
      aatgaaata gtcaactca agtcctgta agtccacaa 
      agtgtatct agaactagc gcaccatca acgagtgct 
      ccaacaaaa gcaaccgaa caagcctcc acagcccaa 
      ctcccgcaa gtctattct aggcaagca aaccttctc 
      ccaacttac gaaatattc agaacttcg cgacaattc 
      gacgaatcg caaagcgat gtcgctgtt gagcaatac 
      ttggcccgc cttcgggct gcaaagaca agcagaccg 
      ccaacgccg tcgcccccc tgtgtgccc cccagctac 
      tccacggta ataaagcag gggcgcccc tcgctagat 
      ataaggagg gatgtgagg tcaatggaa atgcagcac 
      attagcccc ttttcgaga atcccgcca ccgtcttat 
      aaccaaata catggtgtg tggtctcgg gaaattagc 
      aacaattct tctgaggaa gtgttgcta gggccagac 
      cctatatac atttggtgc tcacactgt gccactgtt 
      gttattaca gcgacacag gctcagcgt tcgaagttt 
      acacatatt acagctgcg gccttgtgt ctttttggt 
      tgttggcct tgcgcgatg ttaccctac tgcatgaat 
      tcatgcaag aatatttac cattattgc ccagattgc 
      aattacttg tttgggata aaaaattgt ccaaaaaca 
      ttgacaata ttgaacatg tctcgtttg gtcgttaaa 
      aatttacct aaaaatata actgaagaa aaactcaga 
      aatttgttc caagagaaa ggcactata actgatgtt 
      cagttaaag tacacccct gagggtaaa tttcgtgag 
      tttgctttc gtgggctac cagaatgaa aatgaagcc 
      caggaagct attaaacac tttgatggc atgttcatg 
      cacaccaga aagataaag gttgagcca tgtgcacta 
      ttaggggat tccaagaaa cctaaagct tggagtaaa 
      tatgcccca gaaagtacc gcatataag aaggctcac 
      ggtatttta acaaaggaa gaagacaat gataaaccc 
      aaagttgca aagaagaat caaaaagaa gatgaaact 
      atagaacta cttaaaaaa tatcaagat gatccaatg 
      tttgaagaa tttttagag cttcacaat cctgaagaa 
      agcaagaaa ttgcgcaaa attatagaa aaacaaaaa 
      gaacacgat gtcaaatca gaaagtgag gaagatgag 
      ggaaagggt agctcttca gaaaatgac tcagatgaa 
      gaaacgcaa gacagatta gctgaccaa aaaatcagt 
      gacttagaa tacatgaaa aaactggta aagaagaaa 
      gaaaaaccg aagaaggaa cccaaaaag ttggattta 
      tttacactg aagttgaga ggccttcca tataattcc 
      agaaagaaa gatataaaa caattccta aaacccgtt 
      actcccttt tctatcaga cttcctgca aaaattcac 
      gggattgcg tatgtgggc tttaagacc gaaaaagac 
      tacaagaaa gccctcctg aaagacaga agtttcatc 
      gccgggaaa agaatttcc gtcatggaa tactccgga 
      aaaaataac accgaacac gaggaaact tcaaataat 
      gttcggaaa aaatgggtg agtcaggag gaggctctc 
      aaaaacgag gaagatatc gcggagacg ggcaaaatt 
      tttgtgaga aatttggca tacactaca acagaagac 
      gatgttgag aatctattt tcaaaattt ggcccgcta 
      gctgaggtg aatttgccg gtggatccc acgtctaaa 
      aaaatcaaa ggctttggc actgtgaca tttgtgatg 
      cctgagcac gcggctaga gcttacggg gaactggat 
      ggctctatt ctgcacggg cgcatggtg caccttctg 
      ccaggaaaa agcaaagat gtggccgat gaaccagac 
      ttagaagaa agtacgaat tttaagaag aagaaggaa 
      gcgaaacag aaatctgaa gctgggtcg tctcataac 
      tggaattcg ctatttttg gggcacaat gcagttgct 
      gaagtcatt gcagacact tacagtact tcaaaggaa 
      aaagttatg gggttacat gatgacggt agcgctgct 
      gtgagactt gcactggga gagacacaa atcgtggca 
      caaactagg aaatatctg gagcaagaa ggcgtccaa 
      cttgatgct ttcagtaac aataaatgt aaaaggtcg 
      aaaacagtg atccttgta aagaatttg ccgaataaa 
      actgaagcc aaggagatt agaacaata tttgaaaag 
      tttggcttg gttggacgg ttgattttg ccccctagt 
      ggcattaca gcaattgtt gaatttctt gaaccgtct 
      gaggcacgg aaagctttc acttgtttg gcttacacg 
      aagtttaaa aatgtgcca ctttattta gagtgggct 
      ccggaaaat tctctaggt gaacgaaaa gagccacct 
      gtaacgaat gaagacact acagaagtt aaacaaagt 
      gaaaataat aatgaagtt gaagaaact gtggaagaa 
      tctgaacct gatactact ttatttgtt aaaaacttg 
      aatttccaa acgactgat gacggtcta aggaagcat 
      tttgaaagt tgtggcaaa cttgcgtac gccagcgtt 
      gcaactaaa aaagacaaa aacgaccca agtaaacgt 
      ctatccatg ggatatggc tttgttagg tttatacat 
      aaaagcagc gctgataag gctctcaaa acccttcag 
      cagagcact ctagacggc aaatcccta gaattgaag 
      agatctgaa cggacgttg aacaacgag gtcaaagtt 
      gttaaaaaa agcaccaaa gcaacgaaa caaacagga 
      accaagatt ttggttcga aatatccca ttccaggct 
      aaccagaag gaaattagg gaacttttt agtgttttt 
      ggcgaaatt aaagcgtta aggctccca aagaaaatg 
      gccttggat gggggctca catcgaggt tttgcgttt 
      gtcgatttt gtggcagcc tctgatgcc aagaatgca 
      tttgaggct ttgtgtcaa agtacccac ttgtacggt 
      cggagacta gttctcgag tgggcctca agtgaggac 
      agtgtggac gatatcagg aagcgaaca gcggctcac 
      ttccaaacc actgaagca gtcaagtcg aagaaaagt 
      gttttaagc attgattaa
    mRNA sequence
    • >TC003078 RA
      aaccccgaa tccaaaata tgtccaagt aatgaacga 
      aaaccaaaa tgcaaaaaa cgaagaaac tcccctcga 
      aattttcga aacagtaac agctctagt aaatgaaat 
      agtcaactc aagtcctgt aagtccaca aagtgtatc 
      tagaactag cgcaccatc aacgagtgc tccaacaaa 
      agcaaccga acaagcctc cacagccca actcccgca 
      agtctattc taggcaagc aaaccttct cccaactta 
      cgaaatatt cagaacttc gcgacaatt cgacgaatc 
      gcaaagcga tgtcgctgt tgagcaata cttggcccg 
      ccttcgggc tgcaaagac aagcagacc gccaacgcc 
      gtcgccccc ctgtgtgcc ccccagcta ctccacggt 
      aataaagca ggggcgccc ctcgctaga tataaggag 
      ggatgtgag gtcaatgga aatgcagca cattagccc 
      cttttcgag aatcccgcc accgtctta taaccaaat 
      acatggtgt gtggtctcg ggaaattag caacaattc 
      ttctgagga agtgttgct agggccaga ccctatata 
      catttggtg ctcacactg tgccactgt tgttattac 
      agcgacaca ggctcagcg ttcgaagtt tacacatat 
      tacagctgc ggccttgtg tctttttgg ttgttggcc 
      ttgcgcgat gttacccta ctgcatgaa ttcatgcaa 
      gaatattta ccattattg cccagattg caattactt 
      gtttgggat aaaaaattg tccaaaaac attgacaat 
      attgaacat gtctcgttt ggtcgttaa aaatttacc 
      taaaaatat aactgaaga aaaactcag aaatttgtt 
      ccaagagaa aggcactat aactgatgt tcagttaaa 
      gtacacccc tgagggtaa atttcgtga gtttgcttt 
      cgtgggcta ccagaatga aaatgaagc ccaggaagc 
      tattaaaca ctttgatgg catgttcat gcacaccag 
      aaagataaa ggttgagcc atgtgcact attagggga 
      ttccaagaa acctaaagc ttggagtaa atatgcccc 
      agaaagtac cgcatataa gaaggctca cggtatttt 
      aacaaagga agaagacaa tgataaacc caaagttgc 
      aaagaagaa tcaaaaaga agatgaaac tatagaact 
      acttaaaaa atatcaaga tgatccaat gtttgaaga 
      atttttaga gcttcacaa tcctgaaga aagcaagaa 
      attgcgcaa aattataga aaaacaaaa agaacacga 
      tgtcaaatc agaaagtga ggaagatga gggaaaggg 
      tagctcttc agaaaatga ctcagatga agaaacgca 
      agacagatt agctgacca aaaaatcag tgacttaga 
      atacatgaa aaaactggt aaagaagaa agaaaaacc 
      gaagaagga acccaaaaa gttggattt atttacact 
      gaagttgag aggccttcc atataattc cagaaagaa 
      agatataaa acaattcct aaaacccgt tactccctt 
      ttctatcag acttcctgc aaaaattca cgggattgc 
      gtatgtggg ctttaagac cgaaaaaga ctacaagaa 
      agccctcct gaaagacag aagtttcat cgccgggaa 
      aagaatttc cgtcatgga atactccgg aaaaaataa 
      caccgaaca cgaggaaac ttcaaataa tgttcggaa 
      aaaatgggt gagtcagga ggaggctct caaaaacga 
      ggaagatat cgcggagac gggcaaaat ttttgtgag 
      aaatttggc atacactac aacagaaga cgatgttga 
      gaatctatt ttcaaaatt tggcccgct agctgaggt 
      gaatttgcc ggtggatcc cacgtctaa aaaaatcaa 
      aggctttgg cactgtgac atttgtgat gcctgagca 
      cgcggctag agcttacgg ggaactgga tggctctat 
      tctgcacgg gcgcatggt gcaccttct gccaggaaa 
      aagcaaaga tgtggccga tgaaccaga cttagaaga 
      aagtacgaa ttttaagaa gaagaagga agcgaaaca 
      gaaatctga agctgggtc gtctcataa ctggaattc 
      gctattttt ggggcacaa tgcagttgc tgaagtcat 
      tgcagacac ttacagtac ttcaaagga aaaagttat 
      ggggttaca tgatgacgg tagcgctgc tgtgagact 
      tgcactggg agagacaca aatcgtggc acaaactag 
      gaaatatct ggagcaaga aggcgtcca acttgatgc 
      tttcagtaa caataaatg taaaaggtc gaaaacagt 
      gatccttgt aaagaattt gccgaataa aactgaagc 
      caaggagat tagaacaat atttgaaaa gtttggctt 
      ggttggacg gttgatttt gccccctag tggcattac 
      agcaattgt tgaatttct tgaaccgtc tgaggcacg 
      gaaagcttt cacttgttt ggcttacac gaagtttaa 
      aaatgtgcc actttattt agagtgggc tccggaaaa 
      ttctctagg tgaacgaaa agagccacc tgtaacgaa 
      tgaagacac tacagaagt taaacaaag tgaaaataa 
      taatgaagt tgaagaaac tgtggaaga atctgaacc 
      tgatactac tttatttgt taaaaactt gaatttcca 
      aacgactga tgacggtct aaggaagca ttttgaaag 
      ttgtggcaa acttgcgta cgccagcgt tgcaactaa 
      aaaagacaa aaacgaccc aagtaaacg tctatccat 
      gggatatgg ctttgttag gtttataca taaaagcag 
      cgctgataa ggctctcaa aacccttca gcagagcac 
      tctagacgg caaatccct agaattgaa gagatctga 
      acggacgtt gaacaacga ggtcaaagt tgttaaaaa 
      aagcaccaa agcaacgaa acaaacagg aaccaagat 
      tttggttcg aaatatccc attccaggc taaccagaa 
      ggaaattag ggaactttt tagtgtttt tggcgaaat 
      taaagcgtt aaggctccc aaagaaaat ggccttgga 
      tgggggctc acatcgagg ttttgcgtt tgtcgattt 
      tgtggcagc ctctgatgc caagaatgc atttgaggc 
      tttgtgtca aagtaccca cttgtacgg tcggagact 
      agttctcga gtgggcctc aagtgagga cagtgtgga 
      cgatatcag gaagcgaac agcggctca cttccaaac 
      cactgaagc agtcaagtc gaagaaaag tgttttaag 
      cattgatta attaaaaat aaaaactac actttgtct 
      agtcaagtt tattacctc tagaataaa ccaacatta 
      caaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY