| General information | |||
| TC003078 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 2625519..2636644 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC003078 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_00511 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 2 (9 dpi)remarks: animals lost,
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 90.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 9 (11 dpi)
eclosion delayed (still prepupa) - Number of animals showing the phenotype: 1 (16 dpi)
pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 1 (19 dpi)
- >TC003078 RA
MNENQNAKN EETPLEIFE TVTALVNEI VNSSPVSPQ SVSRTSAPS TSAPTKATE QASTAQLPQ VYSRQANLL PTYEIFRTS RQFDESQSD VAVEQYLAR LRAAKTSRP PTPSPPCVP PSYSTVIKQ GRPSLDIRR DVRSMEMQH ISPFSRIPP PSYNQIHGV WSREISNNS SEEVLLGPD PIYIWCSHC ATVVITATQ AQRSKFTHI TAAALCLFG CWPCAMLPY CMNSCKNIY HYCPDCNYL FGIKNCPKT LTILNMSRL VVKNLPKNI TEEKLRNLF QEKGTITDV QLKYTPEGK FREFAFVGY QNENEAQEA IKHFDGMFM HTRKIKVEP CALLGDSKK PKAWSKYAP ESTAYKKAH GILTKEEDN DKPKVAKKN QKEDETIEL LKKYQDDPM FEEFLELHN PEESKKLRK IIEKQKEHD VKSESEEDE GKGSSSEND SDEETQDRL ADQKISDLE YMKKLVKKK EKPKKEPKK LDLFTLKLR GLPYNSRKK DIKQFLKPV TPFSIRLPA KIHGIAYVG FKTEKDYKK ALLKDRSFI AGKRISVME YSGKNNTEH EETSNNVRK KWVSQEEAL KNEEDIAET GKIFVRNLA YTTTEDDVE NLFSKFGPL AEVNLPVDP TSKKIKGFG TVTFVMPEH AARAYGELD GSILHGRMV HLLPGKSKD VADEPDLEE STNFKKKKE AKQKSEAGS SHNWNSLFL GHNAVAEVI ADTYSTSKE KVMGLHDDG SAAVRLALG ETQIVAQTR KYLEQEGVQ LDAFSNNKC KRSKTVILV KNLPNKTEA KEIRTIFEK FGLVGRLIL PPSGITAIV EFLEPSEAR KAFTCLAYT KFKNVPLYL EWAPENSLG ERKEPPVTN EDTTEVKQS ENNNEVEET VEESEPDTT LFVKNLNFQ TTDDGLRKH FESCGKLAY ASVATKKDK NDPSKRLSM GYGFVRFIH KSSADKALK TLQQSTLDG KSLELKRSE RTLNNEVKV VKKSTKATK QTGTKILVR NIPFQANQK EIRELFSVF GEIKALRLP KKMALDGGS HRGFAFVDF VAASDAKNA FEALCQSTH LYGRRLVLE WASSEDSVD DIRKRTAAH FQTTEAVKS KKSVLSID
- >TC003078 RA
atgaacgaa aaccaaaat gcaaaaaac gaagaaact cccctcgaa attttcgaa acagtaaca gctctagta aatgaaata gtcaactca agtcctgta agtccacaa agtgtatct agaactagc gcaccatca acgagtgct ccaacaaaa gcaaccgaa caagcctcc acagcccaa ctcccgcaa gtctattct aggcaagca aaccttctc ccaacttac gaaatattc agaacttcg cgacaattc gacgaatcg caaagcgat gtcgctgtt gagcaatac ttggcccgc cttcgggct gcaaagaca agcagaccg ccaacgccg tcgcccccc tgtgtgccc cccagctac tccacggta ataaagcag gggcgcccc tcgctagat ataaggagg gatgtgagg tcaatggaa atgcagcac attagcccc ttttcgaga atcccgcca ccgtcttat aaccaaata catggtgtg tggtctcgg gaaattagc aacaattct tctgaggaa gtgttgcta gggccagac cctatatac atttggtgc tcacactgt gccactgtt gttattaca gcgacacag gctcagcgt tcgaagttt acacatatt acagctgcg gccttgtgt ctttttggt tgttggcct tgcgcgatg ttaccctac tgcatgaat tcatgcaag aatatttac cattattgc ccagattgc aattacttg tttgggata aaaaattgt ccaaaaaca ttgacaata ttgaacatg tctcgtttg gtcgttaaa aatttacct aaaaatata actgaagaa aaactcaga aatttgttc caagagaaa ggcactata actgatgtt cagttaaag tacacccct gagggtaaa tttcgtgag tttgctttc gtgggctac cagaatgaa aatgaagcc caggaagct attaaacac tttgatggc atgttcatg cacaccaga aagataaag gttgagcca tgtgcacta ttaggggat tccaagaaa cctaaagct tggagtaaa tatgcccca gaaagtacc gcatataag aaggctcac ggtatttta acaaaggaa gaagacaat gataaaccc aaagttgca aagaagaat caaaaagaa gatgaaact atagaacta cttaaaaaa tatcaagat gatccaatg tttgaagaa tttttagag cttcacaat cctgaagaa agcaagaaa ttgcgcaaa attatagaa aaacaaaaa gaacacgat gtcaaatca gaaagtgag gaagatgag ggaaagggt agctcttca gaaaatgac tcagatgaa gaaacgcaa gacagatta gctgaccaa aaaatcagt gacttagaa tacatgaaa aaactggta aagaagaaa gaaaaaccg aagaaggaa cccaaaaag ttggattta tttacactg aagttgaga ggccttcca tataattcc agaaagaaa gatataaaa caattccta aaacccgtt actcccttt tctatcaga cttcctgca aaaattcac gggattgcg tatgtgggc tttaagacc gaaaaagac tacaagaaa gccctcctg aaagacaga agtttcatc gccgggaaa agaatttcc gtcatggaa tactccgga aaaaataac accgaacac gaggaaact tcaaataat gttcggaaa aaatgggtg agtcaggag gaggctctc aaaaacgag gaagatatc gcggagacg ggcaaaatt tttgtgaga aatttggca tacactaca acagaagac gatgttgag aatctattt tcaaaattt ggcccgcta gctgaggtg aatttgccg gtggatccc acgtctaaa aaaatcaaa ggctttggc actgtgaca tttgtgatg cctgagcac gcggctaga gcttacggg gaactggat ggctctatt ctgcacggg cgcatggtg caccttctg ccaggaaaa agcaaagat gtggccgat gaaccagac ttagaagaa agtacgaat tttaagaag aagaaggaa gcgaaacag aaatctgaa gctgggtcg tctcataac tggaattcg ctatttttg gggcacaat gcagttgct gaagtcatt gcagacact tacagtact tcaaaggaa aaagttatg gggttacat gatgacggt agcgctgct gtgagactt gcactggga gagacacaa atcgtggca caaactagg aaatatctg gagcaagaa ggcgtccaa cttgatgct ttcagtaac aataaatgt aaaaggtcg aaaacagtg atccttgta aagaatttg ccgaataaa actgaagcc aaggagatt agaacaata tttgaaaag tttggcttg gttggacgg ttgattttg ccccctagt ggcattaca gcaattgtt gaatttctt gaaccgtct gaggcacgg aaagctttc acttgtttg gcttacacg aagtttaaa aatgtgcca ctttattta gagtgggct ccggaaaat tctctaggt gaacgaaaa gagccacct gtaacgaat gaagacact acagaagtt aaacaaagt gaaaataat aatgaagtt gaagaaact gtggaagaa tctgaacct gatactact ttatttgtt aaaaacttg aatttccaa acgactgat gacggtcta aggaagcat tttgaaagt tgtggcaaa cttgcgtac gccagcgtt gcaactaaa aaagacaaa aacgaccca agtaaacgt ctatccatg ggatatggc tttgttagg tttatacat aaaagcagc gctgataag gctctcaaa acccttcag cagagcact ctagacggc aaatcccta gaattgaag agatctgaa cggacgttg aacaacgag gtcaaagtt gttaaaaaa agcaccaaa gcaacgaaa caaacagga accaagatt ttggttcga aatatccca ttccaggct aaccagaag gaaattagg gaacttttt agtgttttt ggcgaaatt aaagcgtta aggctccca aagaaaatg gccttggat gggggctca catcgaggt tttgcgttt gtcgatttt gtggcagcc tctgatgcc aagaatgca tttgaggct ttgtgtcaa agtacccac ttgtacggt cggagacta gttctcgag tgggcctca agtgaggac agtgtggac gatatcagg aagcgaaca gcggctcac ttccaaacc actgaagca gtcaagtcg aagaaaagt gttttaagc attgattaa
- >TC003078 RA
aaccccgaa tccaaaata tgtccaagt aatgaacga aaaccaaaa tgcaaaaaa cgaagaaac tcccctcga aattttcga aacagtaac agctctagt aaatgaaat agtcaactc aagtcctgt aagtccaca aagtgtatc tagaactag cgcaccatc aacgagtgc tccaacaaa agcaaccga acaagcctc cacagccca actcccgca agtctattc taggcaagc aaaccttct cccaactta cgaaatatt cagaacttc gcgacaatt cgacgaatc gcaaagcga tgtcgctgt tgagcaata cttggcccg ccttcgggc tgcaaagac aagcagacc gccaacgcc gtcgccccc ctgtgtgcc ccccagcta ctccacggt aataaagca ggggcgccc ctcgctaga tataaggag ggatgtgag gtcaatgga aatgcagca cattagccc cttttcgag aatcccgcc accgtctta taaccaaat acatggtgt gtggtctcg ggaaattag caacaattc ttctgagga agtgttgct agggccaga ccctatata catttggtg ctcacactg tgccactgt tgttattac agcgacaca ggctcagcg ttcgaagtt tacacatat tacagctgc ggccttgtg tctttttgg ttgttggcc ttgcgcgat gttacccta ctgcatgaa ttcatgcaa gaatattta ccattattg cccagattg caattactt gtttgggat aaaaaattg tccaaaaac attgacaat attgaacat gtctcgttt ggtcgttaa aaatttacc taaaaatat aactgaaga aaaactcag aaatttgtt ccaagagaa aggcactat aactgatgt tcagttaaa gtacacccc tgagggtaa atttcgtga gtttgcttt cgtgggcta ccagaatga aaatgaagc ccaggaagc tattaaaca ctttgatgg catgttcat gcacaccag aaagataaa ggttgagcc atgtgcact attagggga ttccaagaa acctaaagc ttggagtaa atatgcccc agaaagtac cgcatataa gaaggctca cggtatttt aacaaagga agaagacaa tgataaacc caaagttgc aaagaagaa tcaaaaaga agatgaaac tatagaact acttaaaaa atatcaaga tgatccaat gtttgaaga atttttaga gcttcacaa tcctgaaga aagcaagaa attgcgcaa aattataga aaaacaaaa agaacacga tgtcaaatc agaaagtga ggaagatga gggaaaggg tagctcttc agaaaatga ctcagatga agaaacgca agacagatt agctgacca aaaaatcag tgacttaga atacatgaa aaaactggt aaagaagaa agaaaaacc gaagaagga acccaaaaa gttggattt atttacact gaagttgag aggccttcc atataattc cagaaagaa agatataaa acaattcct aaaacccgt tactccctt ttctatcag acttcctgc aaaaattca cgggattgc gtatgtggg ctttaagac cgaaaaaga ctacaagaa agccctcct gaaagacag aagtttcat cgccgggaa aagaatttc cgtcatgga atactccgg aaaaaataa caccgaaca cgaggaaac ttcaaataa tgttcggaa aaaatgggt gagtcagga ggaggctct caaaaacga ggaagatat cgcggagac gggcaaaat ttttgtgag aaatttggc atacactac aacagaaga cgatgttga gaatctatt ttcaaaatt tggcccgct agctgaggt gaatttgcc ggtggatcc cacgtctaa aaaaatcaa aggctttgg cactgtgac atttgtgat gcctgagca cgcggctag agcttacgg ggaactgga tggctctat tctgcacgg gcgcatggt gcaccttct gccaggaaa aagcaaaga tgtggccga tgaaccaga cttagaaga aagtacgaa ttttaagaa gaagaagga agcgaaaca gaaatctga agctgggtc gtctcataa ctggaattc gctattttt ggggcacaa tgcagttgc tgaagtcat tgcagacac ttacagtac ttcaaagga aaaagttat ggggttaca tgatgacgg tagcgctgc tgtgagact tgcactggg agagacaca aatcgtggc acaaactag gaaatatct ggagcaaga aggcgtcca acttgatgc tttcagtaa caataaatg taaaaggtc gaaaacagt gatccttgt aaagaattt gccgaataa aactgaagc caaggagat tagaacaat atttgaaaa gtttggctt ggttggacg gttgatttt gccccctag tggcattac agcaattgt tgaatttct tgaaccgtc tgaggcacg gaaagcttt cacttgttt ggcttacac gaagtttaa aaatgtgcc actttattt agagtgggc tccggaaaa ttctctagg tgaacgaaa agagccacc tgtaacgaa tgaagacac tacagaagt taaacaaag tgaaaataa taatgaagt tgaagaaac tgtggaaga atctgaacc tgatactac tttatttgt taaaaactt gaatttcca aacgactga tgacggtct aaggaagca ttttgaaag ttgtggcaa acttgcgta cgccagcgt tgcaactaa aaaagacaa aaacgaccc aagtaaacg tctatccat gggatatgg ctttgttag gtttataca taaaagcag cgctgataa ggctctcaa aacccttca gcagagcac tctagacgg caaatccct agaattgaa gagatctga acggacgtt gaacaacga ggtcaaagt tgttaaaaa aagcaccaa agcaacgaa acaaacagg aaccaagat tttggttcg aaatatccc attccaggc taaccagaa ggaaattag ggaactttt tagtgtttt tggcgaaat taaagcgtt aaggctccc aaagaaaat ggccttgga tgggggctc acatcgagg ttttgcgtt tgtcgattt tgtggcagc ctctgatgc caagaatgc atttgaggc tttgtgtca aagtaccca cttgtacgg tcggagact agttctcga gtgggcctc aagtgagga cagtgtgga cgatatcag gaagcgaac agcggctca cttccaaac cactgaagc agtcaagtc gaagaaaag tgttttaag cattgatta attaaaaat aaaaactac actttgtct agtcaagtt tattacctc tagaataaa ccaacatta caaaa
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
