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Gene information
General information
TC003070 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid14: 1680068..1700843
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003070
    iBeetle screen iB_00510
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: 4 - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 90.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (16 dpi)
    Days post injection



    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003070 RA
      MTSFKSKIT NRKQKNSKS VNSQSESAE SESAPTGKN 
      QQKSGKHRN RGGKKAPPS AETTVTPPS DIIASTDLE 
      LADVVLRTS VPSEVAEQN LKNSNQKRF SDGFVIENG 
      ANVRLTRAV SGILGPEEL YTGDKTAKK GRRLSDLFR 
      HGTLKATNS LENLNMKHS DHLIKEPLN KATADSKTK 
      RHKKGSSYP LANNKEAKN AKNSEAAQA TKQNSENNS 
      YLKRVKSKI YKTKSETNA LLPSKTTVS KKSSQCERI 
      PEDVELRKS NFDFRLIRQ SSNLEQVRS RTFGLMKSS 
      SNTGIADME DGIAKKPLL AKAKSSSSI NLNLLRMRR 
      SRILDQLQE GRCKDVFDE FDFIAFGGF RDKLGGSQK 
      LATADARID TDLGENKPS QPVSRSGSD RRPDIVREE 
      TPKNAEGTT STDHGPNKQ ENETSAPDN VASFPQASH 
      KKKSSSNIN RSESVKEQS EKRKQRRNI SDPSHNTTS 
      GDVELDQPG LSNTDSGSS SNSSISYNG RLSESPSNS 
      VDVVTTDGG DRKPSSDWE SDAEMEPAN WQKLFPEEE 
      LRNLQPHEK KRQDVINEL FHTESSHVR NLRVLCEIF 
      YKTLKESQV LKPDEIALI FPNIKEMLD VHTQFNREM 
      KRIRREDPL VSNLGDMLV KMFGGVVGD MLKRAAAVF 
      CERQQQALE FIKERRKRD SKFDGVLVE CEKKRQCRR 
      LPLQGIIPT EMQRLSKYP LLLERLISS VEANTSSSH 
      AQEELVKLK RAHHFSKDI LNHVNEAAK VAFNKSRLD 
      EIQRHLDTT NFERSDHPI AQEFRMLDL TQYKLIREG 
      NMHLRRGNK PMVPVHVLL LEEAVVILH KEGDKFFLK 
      FFQSGSSAQ PQPLSPLIK MSTLLVRNN AACKRALFL 
      FNTSTNGTQ MYDLLADDD INRDVWLKH FSDAADAYN 
      RREGKTKRH ESQPDSDES ETLQEIPST RDVAERAEA 
      VGGTDNTPD DEEEDEQIF SDAQEEGST EQKEVPSSS 
      EGGGVHTKV TADEWPLIQ PSQVHVEVP PVHTAESRL 
      TPLEQIRRK DEEVRRALE DKEGLVADL LSIPREHFQ 
      LIADMASDT QDTRSSKDL AERVLAAVY QVDQLHLAV 
      NEALNVTEA SAVSATGGK NPACQSQEL LPTVSPPSI 
      PANRVRDVA NSLSSQLTT LLSDVKQVE EERDRLRKE 
      LFRMRERLH EQQMLHGPA ENNVESPTP ELPPDLDSI 
      KESPAEPES
    CDS sequence
    • >TC003070 RA
      atgacatca tttaaaagt aagataacg aaccggaaa 
      cagaaaaac tcaaagtct gtgaatagt cagtcggag 
      agtgccgag agtgagagt gcgccgacc ggaaaaaac 
      cagcaaaag tccgggaag catcgcaat cgcggcggt 
      aaaaaagca ccaccgagt gcagaaacc actgttacg 
      ccaccaagt gacatcatt gcaagcacc gacctcgag 
      ctcgcagat gttgtgcta aggacaagc gttccgagc 
      gaagtcgcc gaacagaac cttaaaaat agcaatcaa 
      aagaggttc agcgacggc tttgtcatt gaaaatggg 
      gcaaacgtg cgtttgacg cgagcggtg agcggaatt 
      ctcggaccc gaagaattg tacaccggc gataaaaca 
      gcaaagaaa gggagacgc ttgagtgat ctgtttaga 
      cacggtacg cttaaagcc acaaatagc ctagaaaac 
      ttaaatatg aaacattcg gatcatttg attaaagaa 
      ccgctgaac aaagcgacc gcagattcg aagaccaag 
      cggcataaa aagggctca agctacccg ctggcgaat 
      aataaagaa gcaaagaac gccaaaaat tcggaggcg 
      gcgcaagcc accaaacaa aactccgag aacaattcg 
      tacttaaaa cgagtcaag tcaaagatt tacaaaact 
      aaatccgaa actaacgct ttactaccg tccaaaaca 
      accgtctcg aagaaaagc tcgcaatgt gaacgcatc 
      cccgaagac gtcgagctg cgaaagtca aatttcgac 
      ttccgcctg attcggcaa tcctccaac ttggagcaa 
      gtccggagc cgaacgttc ggcctcatg aagtcatct 
      tccaatacc ggcatcgct gatatggaa gatggcatt 
      gcgaaaaag ccactcctt gccaaagca aagtcaagt 
      agttccata aatctcaac cttttgagg atgcggcgc 
      agccgtata cttgaccag ttgcaggaa ggccggtgt 
      aaagacgtt tttgatgag tttgatttt attgcattc 
      ggcgggttt agggataag ttaggcgga tcgcaaaaa 
      ctagctaca gcagacgcg aggatcgat actgactta 
      ggagaaaat aagcccagt caacccgtg tcacgaagc 
      ggatccgac cgaaggccg gatatagta cgtgaagag 
      acacccaaa aatgctgaa gggacgacc agcaccgac 
      catggcccc aataagcag gaaaacgaa acctccgcc 
      cctgacaat gtcgctagt ttcccgcaa gcctctcat 
      aagaagaag tctagcagt aacataaac cgatcggaa 
      agcgtgaaa gagcagtcc gaaaagcgg aaacaaaga 
      cggaacatt agcgatccc tctcacaat acgacaagt 
      ggcgatgtg gagttggat cagccaggc ttgtcaaat 
      acagattcc ggtagttca tccaattct agcatatcc 
      tacaatggc cgtttgtca gagagtcct agcaatagt 
      gtggacgtc gtcaccacc gatggaggc gacaggaag 
      cccagttca gactgggaa agtgatgcc gaaatggaa 
      cccgccaat tggcagaaa ttgttccca gaagaagaa 
      ctacgtaac ctccagcca cacgaaaag aaaaggcaa 
      gacgtaatt aacgaactg tttcacacg gagagttcc 
      catgtacgg aaccttcgc gtactgtgt gagattttt 
      tacaagact cttaaggaa agtcaggtt ttaaagccg 
      gacgaaata gctttaatt tttccaaat atcaaagaa 
      atgctcgat gtgcatacg cagttcaat agagagatg 
      aagaggata cgacgggag gatccctta gtgagcaat 
      ctcggtgac atgttagtc aaaatgttt ggcggtgtt 
      gtgggcgat atgcttaag cgagccgcc gcagttttc 
      tgcgaacgc cagcagcaa gctctcgaa tttatcaag 
      gaacggcgt aaacgcgac tcgaaattt gatggggtt 
      ttagtcgag tgcgagaaa aagcggcaa tgtaggcga 
      ttaccactc caagggatc atcccaact gaaatgcaa 
      cgactgtct aaatatccc cttttattg gagcggctg 
      attagcagc gtcgaggcc aacacttcg agctcacat 
      gcgcaggag gagttagtg aaactgaaa cgtgcgcac 
      cacttttcc aaagacatt ttaaaccac gttaacgaa 
      gctgctaaa gtggccttt aacaaatca cgacttgac 
      gaaatacag cggcatcta gacacgacg aattttgaa 
      cggtcggat catccaata gcgcaagag tttaggatg 
      ttagatcta acgcagtac aaactaatc agagaaggt 
      aatatgcac ctacgaaga gggaacaaa ccaatggtg 
      cctgttcac gtgctgtta ctagaagaa gctgtcgtt 
      attttacat aaagaaggc gataagttc ttcctaaag 
      ttcttccaa tcaggttcc tcggcacag cctcaaccc 
      ctctcacct cttataaag atgtccaca ttgttagta 
      cggaataat gcggcctgt aaaagagct ctcttccta 
      ttcaatact tccacaaac ggcactcaa atgtacgat 
      ctcctagca gacgacgat attaataga gacgtgtgg 
      ctgaaacat ttctcggac gcagccgat gcgtataat 
      cggagagaa gggaaaacg aaaaggcat gagtcgcag 
      cctgactca gacgagtcg gaaactttg caggaaatt 
      cccagtacg cgagatgtt gccgagagg gctgaagcc 
      gtcggtgga acggataat actcctgat gatgaagag 
      gaggacgaa caaatcttc agtgatgct caggaggaa 
      ggatctacg gagcagaag gaggttccg agctcgtcg 
      gaaggtggc ggtgttcac accaaagta acagcggat 
      gagtggcct ctcatccag ccttcacag gtccacgta 
      gaagtacca ccagtgcac actgctgag tcaaggtta 
      actccacta gaacaaatt cgtcgtaaa gatgaagaa 
      gtgcgccga gcgttagaa gacaaagag ggcctggtt 
      gccgacctt ttgagtatt cctcgggag cacttccaa 
      cttattgct gatatggcc agtgataca caagacacg 
      cggtcttcc aaagaccta gcggagcgc gtattggct 
      gccgtatat caagtggat cagctgcac ctagccgtc 
      aatgaagcg ctgaatgtg acggaggct tcagctgta 
      tcagcgaca ggtggcaaa aacccagct tgccaaagc 
      caggaattg ttgccaacc gtttcacca ccttccatt 
      cctgccaat cgtgttaga gacgttgcc aactcgctg 
      agttcacag ctaacgacc ctacttagt gatgtgaaa 
      caagttgag gaggagcgc gatcggctt aggaaggag 
      ctcttccgc atgagggag cgactgcat gagcagcag 
      atgcttcac gggcctgca gaaaacaac gtagaatcg 
      cctacacca gaactccca ccagacttg gacagtatt 
      aaggaaagt ccagccgag ccagagagc taa
    mRNA sequence
    • >TC003070 RA
      aaattctcc ggtcattag catggacat aatatctag 
      aacatggct gcgatattg ttgcggctt cgaaagaaa 
      ctttcctga cgaaaaact taaaaaatg cagctgggt 
      ttttagcga aatacgcag tgcagtgaa acgactgca 
      tggaaaagt aaattcgcc agcttatcg cgggtgtag 
      tcggcatta ataaagtct gataagcag cgtggaaag 
      tgtaagtgg ggacttgag ccttgtgcg gcaaaatgt 
      ctggaaacg gaacgcggg gaacgtttt agttgctgg 
      ctggtgatg tttttagtt cgccagttg tctggctaa 
      cgtgaactc agacaaaga acaacgctc tctccgtgc 
      ttgtggctc tgttcgacc ggtaatccg cgaaattcc 
      ctctgggga actgaccgt tgaggtatc cccagtacg 
      tttatgacg gaatctatt gaatcatgt gttaggaat 
      ccggtggta ttgcaagtg attcttctt gggcgttgg 
      atgtgaggt taagtgccg ccatgacaa cacgcatag 
      gggcataac aacaatcgt gcgtgaggt gtttttgga 
      acactttac gtaaactgg tggcggcgc cgttttaag 
      tgcttcagc gaatttaac tttttatcg cacgtatgt 
      tagcctgct atcggcatt cgtcatcct tgcgcttaa 
      ttagcagtg gtctgtgtt gtatcattt aattgttta 
      atcggcgga ttccgcaaa ctccgccat ttattttct 
      gatcgatcg ggggtttac ccgagaaaa ttcgtactc 
      gtgtgtatt aaaaattcc aatcagatc accggatgt 
      gggtgcctc cgactgtaa taatacagt gatgatttc 
      aattgttgg cacatagct ttaaatttg caacgtgtg 
      ttaaactgt agttggcaa aatgggaat tttcagcct 
      ttgcataaa ttattaata aacaacaag tgcgtttgg 
      taaaataat gtggtcaaa gcaagcgga tatgcgtaa 
      cgcaaacat gacatcatt taaaagtaa gataacgaa 
      ccggaaaca gaaaaactc aaagtctgt gaatagtca 
      gtcggagag tgccgagag tgagagtgc gccgaccgg 
      aaaaaacca gcaaaagtc cgggaagca tcgcaatcg 
      cggcggtaa aaaagcacc accgagtgc agaaaccac 
      tgttacgcc accaagtga catcattgc aagcaccga 
      cctcgagct cgcagatgt tgtgctaag gacaagcgt 
      tccgagcga agtcgccga acagaacct taaaaatag 
      caatcaaaa gaggttcag cgacggctt tgtcattga 
      aaatggggc aaacgtgcg tttgacgcg agcggtgag 
      cggaattct cggacccga agaattgta caccggcga 
      taaaacagc aaagaaagg gagacgctt gagtgatct 
      gtttagaca cggtacgct taaagccac aaatagcct 
      agaaaactt aaatatgaa acattcgga tcatttgat 
      taaagaacc gctgaacaa agcgaccgc agattcgaa 
      gaccaagcg gcataaaaa gggctcaag ctacccgct 
      ggcgaataa taaagaagc aaagaacgc caaaaattc 
      ggaggcggc gcaagccac caaacaaaa ctccgagaa 
      caattcgta cttaaaacg agtcaagtc aaagattta 
      caaaactaa atccgaaac taacgcttt actaccgtc 
      caaaacaac cgtctcgaa gaaaagctc gcaatgtga 
      acgcatccc cgaagacgt cgagctgcg aaagtcaaa 
      tttcgactt ccgcctgat tcggcaatc ctccaactt 
      ggagcaagt ccggagccg aacgttcgg cctcatgaa 
      gtcatcttc caataccgg catcgctga tatggaaga 
      tggcattgc gaaaaagcc actccttgc caaagcaaa 
      gtcaagtag ttccataaa tctcaacct tttgaggat 
      gcggcgcag ccgtatact tgaccagtt gcaggaagg 
      ccggtgtaa agacgtttt tgatgagtt tgattttat 
      tgcattcgg cgggtttag ggataagtt aggcggatc 
      gcaaaaact agctacagc agacgcgag gatcgatac 
      tgacttagg agaaaataa gcccagtca acccgtgtc 
      acgaagcgg atccgaccg aaggccgga tatagtacg 
      tgaagagac acccaaaaa tgctgaagg gacgaccag 
      caccgacca tggccccaa taagcagga aaacgaaac 
      ctccgcccc tgacaatgt cgctagttt cccgcaagc 
      ctctcataa gaagaagtc tagcagtaa cataaaccg 
      atcggaaag cgtgaaaga gcagtccga aaagcggaa 
      acaaagacg gaacattag cgatccctc tcacaatac 
      gacaagtgg cgatgtgga gttggatca gccaggctt 
      gtcaaatac agattccgg tagttcatc caattctag 
      catatccta caatggccg tttgtcaga gagtcctag 
      caatagtgt ggacgtcgt caccaccga tggaggcga 
      caggaagcc cagttcaga ctgggaaag tgatgccga 
      aatggaacc cgccaattg gcagaaatt gttcccaga 
      agaagaact acgtaacct ccagccaca cgaaaagaa 
      aaggcaaga cgtaattaa cgaactgtt tcacacgga 
      gagttccca tgtacggaa ccttcgcgt actgtgtga 
      gatttttta caagactct taaggaaag tcaggtttt 
      aaagccgga cgaaatagc tttaatttt tccaaatat 
      caaagaaat gctcgatgt gcatacgca gttcaatag 
      agagatgaa gaggatacg acgggagga tcccttagt 
      gagcaatct cggtgacat gttagtcaa aatgtttgg 
      cggtgttgt gggcgatat gcttaagcg agccgccgc 
      agttttctg cgaacgcca gcagcaagc tctcgaatt 
      tatcaagga acggcgtaa acgcgactc gaaatttga 
      tggggtttt agtcgagtg cgagaaaaa gcggcaatg 
      taggcgatt accactcca agggatcat cccaactga 
      aatgcaacg actgtctaa atatcccct tttattgga 
      gcggctgat tagcagcgt cgaggccaa cacttcgag 
      ctcacatgc gcaggagga gttagtgaa actgaaacg 
      tgcgcacca cttttccaa agacatttt aaaccacgt 
      taacgaagc tgctaaagt ggcctttaa caaatcacg 
      acttgacga aatacagcg gcatctaga cacgacgaa 
      ttttgaacg gtcggatca tccaatagc gcaagagtt 
      taggatgtt agatctaac gcagtacaa actaatcag 
      agaaggtaa tatgcacct acgaagagg gaacaaacc 
      aatggtgcc tgttcacgt gctgttact agaagaagc 
      tgtcgttat tttacataa agaaggcga taagttctt 
      cctaaagtt cttccaatc aggttcctc ggcacagcc 
      tcaacccct ctcacctct tataaagat gtccacatt 
      gttagtacg gaataatgc ggcctgtaa aagagctct 
      cttcctatt caatacttc cacaaacgg cactcaaat 
      gtacgatct cctagcaga cgacgatat taatagaga 
      cgtgtggct gaaacattt ctcggacgc agccgatgc 
      gtataatcg gagagaagg gaaaacgaa aaggcatga 
      gtcgcagcc tgactcaga cgagtcgga aactttgca 
      ggaaattcc cagtacgcg agatgttgc cgagagggc 
      tgaagccgt cggtggaac ggataatac tcctgatga 
      tgaagagga ggacgaaca aatcttcag tgatgctca 
      ggaggaagg atctacgga gcagaagga ggttccgag 
      ctcgtcgga aggtggcgg tgttcacac caaagtaac 
      agcggatga gtggcctct catccagcc ttcacaggt 
      ccacgtaga agtaccacc agtgcacac tgctgagtc 
      aaggttaac tccactaga acaaattcg tcgtaaaga 
      tgaagaagt gcgccgagc gttagaaga caaagaggg 
      cctggttgc cgacctttt gagtattcc tcgggagca 
      cttccaact tattgctga tatggccag tgatacaca 
      agacacgcg gtcttccaa agacctagc ggagcgcgt 
      attggctgc cgtatatca agtggatca gctgcacct 
      agccgtcaa tgaagcgct gaatgtgac ggaggcttc 
      agctgtatc agcgacagg tggcaaaaa cccagcttg 
      ccaaagcca ggaattgtt gccaaccgt ttcaccacc 
      ttccattcc tgccaatcg tgttagaga cgttgccaa 
      ctcgctgag ttcacagct aacgaccct acttagtga 
      tgtgaaaca agttgagga ggagcgcga tcggcttag 
      gaaggagct cttccgcat gagggagcg actgcatga 
      gcagcagat gcttcacgg gcctgcaga aaacaacgt 
      agaatcgcc tacaccaga actcccacc agacttgga 
      cagtattaa ggaaagtcc agccgagcc agagagcta 
      acgtgataa gaacactca atatagggc cttgaatat 
      cgctaaaca gacaatttg tataaagaa ggaattata 
      tttgttata tgctaattt tattattat tatctttta 
      ggtaatctt gtgaaagag cctaggtat atttcttat 
      tcatgttat ttttataaa taactgaag gaggtgtgt 
      tttgatacg gcatgtcct tctgagtta atgtgaaca 
      tgatatacg tttttctcc cttcttttt aatgggtgc 
      aataatgct ttcactgtt gttcattat tataagtcg 
      ctagtactc aatttgaca aggcctaag acggtctaa 
      aaattattt aaatgaatg tatattaac aataagtat 
      tacgctgtc tacaaagtc attttgttt gtagctatt 
      aatttgtac actacgagt ttacacaat tgttatctt 
      cagtgtcta ggcggtttt ccaagttga gaaggaacg 
      gctggttaa ctactccac ttcaatagt tataaacaa 
      tttgattcg tgtaagagt tacgatgta tgttttgtt 
      tttatatgt atgttgtct actttgtta cctcatttt 
      acaatgtta tttaatgag ataatcaca acttgttaa 
      atgttgtct agctttttt ataaccgct ttttaaaaa 
      tgcattttc gttataaga tcgcattta tagatgtaa 
      agtgtatca ttttttact ttattgtat ctacatatt 
      tatagcgaa ttatataat aatgaatga catcgcaat 
      tattaaaaa cttgttaag tacacatat ttatataaa 
      aaataataa tgctacata ataaactat ttaaaacat 
      c