Status
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Gene information
General information
TC003070 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 2758579..2779354
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC003070
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00510

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: 4 - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 90.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection

    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection

    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection

    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003070 RA
      MTSFKSKIT NRKQKNSKS VNSQSESAE SESAPTGKN 
QQKSGKHRN RGGKKAPPS AETTVTPPS DIIASTDLE 
LADVVLRTS VPSEVAEQN LKNSNQKRF SDGFVIENG 
ANVRLTRAV SGILGPEEL YTGDKTAKK GRRLSDLFR 
HGTLKATNS LENLNMKHS DHLIKEPLN KATADSKTK 
RHKKGSSYP LANNKEAKN AKNSEAAQA TKQNSENNS 
YLKRVKSKI YKTKSETNA LLPSKTTVS KKSSQCERI 
PEDVELRKS NFDFRLIRQ SSNLEQVRS RTFGLMKSS 
SNTGIADME DGIAKKPLL AKAKSSSSI NLNLLRMRR 
SRILDQLQE GRCKDVFDE FDFIAFGGF RDKLGGSQK 
LATADARID TDLGENKPS QPVSRSGSD RRPDIVREE 
TPKNAEGTT STDHGPNKQ ENETSAPDN VASFPQASH 
KKKSSSNIN RSESVKEQS EKRKQRRNI SDPSHNTTS 
GDVELDQPG LSNTDSGSS SNSSISYNG RLSESPSNS 
VDVVTTDGG DRKPSSDWE SDAEMEPAN WQKLFPEEE 
LRNLQPHEK KRQDVINEL FHTESSHVR NLRVLCEIF 
YKTLKESQV LKPDEIALI FPNIKEMLD VHTQFNREM 
KRIRREDPL VSNLGDMLV KMFGGVVGD MLKRAAAVF 
CERQQQALE FIKERRKRD SKFDGVLVE CEKKRQCRR 
LPLQGIIPT EMQRLSKYP LLLERLISS VEANTSSSH 
AQEELVKLK RAHHFSKDI LNHVNEAAK VAFNKSRLD 
EIQRHLDTT NFERSDHPI AQEFRMLDL TQYKLIREG 
NMHLRRGNK PMVPVHVLL LEEAVVILH KEGDKFFLK 
FFQSGSSAQ PQPLSPLIK MSTLLVRNN AACKRALFL 
FNTSTNGTQ MYDLLADDD INRDVWLKH FSDAADAYN 
RREGKTKRH ESQPDSDES ETLQEIPST RDVAERAEA 
VGGTDNTPD DEEEDEQIF SDAQEEGST EQKEVPSSS 
EGGGVHTKV TADEWPLIQ PSQVHVEVP PVHTAESRL 
TPLEQIRRK DEEVRRALE DKEGLVADL LSIPREHFQ 
LIADMASDT QDTRSSKDL AERVLAAVY QVDQLHLAV 
NEALNVTEA SAVSATGGK NPACQSQEL LPTVSPPSI 
PANRVRDVA NSLSSQLTT LLSDVKQVE EERDRLRKE 
LFRMRERLH EQQMLHGPA ENNVESPTP ELPPDLDSI 
KESPAEPES
    CDS sequence
    • >TC003070 RA
      atgacatca tttaaaagt aagataacg aaccggaaa 
cagaaaaac tcaaagtct gtgaatagt cagtcggag 
agtgccgag agtgagagt gcgccgacc ggaaaaaac 
cagcaaaag tccgggaag catcgcaat cgcggcggt 
aaaaaagca ccaccgagt gcagaaacc actgttacg 
ccaccaagt gacatcatt gcaagcacc gacctcgag 
ctcgcagat gttgtgcta aggacaagc gttccgagc 
gaagtcgcc gaacagaac cttaaaaat agcaatcaa 
aagaggttc agcgacggc tttgtcatt gaaaatggg 
gcaaacgtg cgtttgacg cgagcggtg agcggaatt 
ctcggaccc gaagaattg tacaccggc gataaaaca 
gcaaagaaa gggagacgc ttgagtgat ctgtttaga 
cacggtacg cttaaagcc acaaatagc ctagaaaac 
ttaaatatg aaacattcg gatcatttg attaaagaa 
ccgctgaac aaagcgacc gcagattcg aagaccaag 
cggcataaa aagggctca agctacccg ctggcgaat 
aataaagaa gcaaagaac gccaaaaat tcggaggcg 
gcgcaagcc accaaacaa aactccgag aacaattcg 
tacttaaaa cgagtcaag tcaaagatt tacaaaact 
aaatccgaa actaacgct ttactaccg tccaaaaca 
accgtctcg aagaaaagc tcgcaatgt gaacgcatc 
cccgaagac gtcgagctg cgaaagtca aatttcgac 
ttccgcctg attcggcaa tcctccaac ttggagcaa 
gtccggagc cgaacgttc ggcctcatg aagtcatct 
tccaatacc ggcatcgct gatatggaa gatggcatt 
gcgaaaaag ccactcctt gccaaagca aagtcaagt 
agttccata aatctcaac cttttgagg atgcggcgc 
agccgtata cttgaccag ttgcaggaa ggccggtgt 
aaagacgtt tttgatgag tttgatttt attgcattc 
ggcgggttt agggataag ttaggcgga tcgcaaaaa 
ctagctaca gcagacgcg aggatcgat actgactta 
ggagaaaat aagcccagt caacccgtg tcacgaagc 
ggatccgac cgaaggccg gatatagta cgtgaagag 
acacccaaa aatgctgaa gggacgacc agcaccgac 
catggcccc aataagcag gaaaacgaa acctccgcc 
cctgacaat gtcgctagt ttcccgcaa gcctctcat 
aagaagaag tctagcagt aacataaac cgatcggaa 
agcgtgaaa gagcagtcc gaaaagcgg aaacaaaga 
cggaacatt agcgatccc tctcacaat acgacaagt 
ggcgatgtg gagttggat cagccaggc ttgtcaaat 
acagattcc ggtagttca tccaattct agcatatcc 
tacaatggc cgtttgtca gagagtcct agcaatagt 
gtggacgtc gtcaccacc gatggaggc gacaggaag 
cccagttca gactgggaa agtgatgcc gaaatggaa 
cccgccaat tggcagaaa ttgttccca gaagaagaa 
ctacgtaac ctccagcca cacgaaaag aaaaggcaa 
gacgtaatt aacgaactg tttcacacg gagagttcc 
catgtacgg aaccttcgc gtactgtgt gagattttt 
tacaagact cttaaggaa agtcaggtt ttaaagccg 
gacgaaata gctttaatt tttccaaat atcaaagaa 
atgctcgat gtgcatacg cagttcaat agagagatg 
aagaggata cgacgggag gatccctta gtgagcaat 
ctcggtgac atgttagtc aaaatgttt ggcggtgtt 
gtgggcgat atgcttaag cgagccgcc gcagttttc 
tgcgaacgc cagcagcaa gctctcgaa tttatcaag 
gaacggcgt aaacgcgac tcgaaattt gatggggtt 
ttagtcgag tgcgagaaa aagcggcaa tgtaggcga 
ttaccactc caagggatc atcccaact gaaatgcaa 
cgactgtct aaatatccc cttttattg gagcggctg 
attagcagc gtcgaggcc aacacttcg agctcacat 
gcgcaggag gagttagtg aaactgaaa cgtgcgcac 
cacttttcc aaagacatt ttaaaccac gttaacgaa 
gctgctaaa gtggccttt aacaaatca cgacttgac 
gaaatacag cggcatcta gacacgacg aattttgaa 
cggtcggat catccaata gcgcaagag tttaggatg 
ttagatcta acgcagtac aaactaatc agagaaggt 
aatatgcac ctacgaaga gggaacaaa ccaatggtg 
cctgttcac gtgctgtta ctagaagaa gctgtcgtt 
attttacat aaagaaggc gataagttc ttcctaaag 
ttcttccaa tcaggttcc tcggcacag cctcaaccc 
ctctcacct cttataaag atgtccaca ttgttagta 
cggaataat gcggcctgt aaaagagct ctcttccta 
ttcaatact tccacaaac ggcactcaa atgtacgat 
ctcctagca gacgacgat attaataga gacgtgtgg 
ctgaaacat ttctcggac gcagccgat gcgtataat 
cggagagaa gggaaaacg aaaaggcat gagtcgcag 
cctgactca gacgagtcg gaaactttg caggaaatt 
cccagtacg cgagatgtt gccgagagg gctgaagcc 
gtcggtgga acggataat actcctgat gatgaagag 
gaggacgaa caaatcttc agtgatgct caggaggaa 
ggatctacg gagcagaag gaggttccg agctcgtcg 
gaaggtggc ggtgttcac accaaagta acagcggat 
gagtggcct ctcatccag ccttcacag gtccacgta 
gaagtacca ccagtgcac actgctgag tcaaggtta 
actccacta gaacaaatt cgtcgtaaa gatgaagaa 
gtgcgccga gcgttagaa gacaaagag ggcctggtt 
gccgacctt ttgagtatt cctcgggag cacttccaa 
cttattgct gatatggcc agtgataca caagacacg 
cggtcttcc aaagaccta gcggagcgc gtattggct 
gccgtatat caagtggat cagctgcac ctagccgtc 
aatgaagcg ctgaatgtg acggaggct tcagctgta 
tcagcgaca ggtggcaaa aacccagct tgccaaagc 
caggaattg ttgccaacc gtttcacca ccttccatt 
cctgccaat cgtgttaga gacgttgcc aactcgctg 
agttcacag ctaacgacc ctacttagt gatgtgaaa 
caagttgag gaggagcgc gatcggctt aggaaggag 
ctcttccgc atgagggag cgactgcat gagcagcag 
atgcttcac gggcctgca gaaaacaac gtagaatcg 
cctacacca gaactccca ccagacttg gacagtatt 
aaggaaagt ccagccgag ccagagagc taa
    mRNA sequence
    • >TC003070 RA
      aaattctcc ggtcattag catggacat aatatctag 
aacatggct gcgatattg ttgcggctt cgaaagaaa 
ctttcctga cgaaaaact taaaaaatg cagctgggt 
ttttagcga aatacgcag tgcagtgaa acgactgca 
tggaaaagt aaattcgcc agcttatcg cgggtgtag 
tcggcatta ataaagtct gataagcag cgtggaaag 
tgtaagtgg ggacttgag ccttgtgcg gcaaaatgt 
ctggaaacg gaacgcggg gaacgtttt agttgctgg 
ctggtgatg tttttagtt cgccagttg tctggctaa 
cgtgaactc agacaaaga acaacgctc tctccgtgc 
ttgtggctc tgttcgacc ggtaatccg cgaaattcc 
ctctgggga actgaccgt tgaggtatc cccagtacg 
tttatgacg gaatctatt gaatcatgt gttaggaat 
ccggtggta ttgcaagtg attcttctt gggcgttgg 
atgtgaggt taagtgccg ccatgacaa cacgcatag 
gggcataac aacaatcgt gcgtgaggt gtttttgga 
acactttac gtaaactgg tggcggcgc cgttttaag 
tgcttcagc gaatttaac tttttatcg cacgtatgt 
tagcctgct atcggcatt cgtcatcct tgcgcttaa 
ttagcagtg gtctgtgtt gtatcattt aattgttta 
atcggcgga ttccgcaaa ctccgccat ttattttct 
gatcgatcg ggggtttac ccgagaaaa ttcgtactc 
gtgtgtatt aaaaattcc aatcagatc accggatgt 
gggtgcctc cgactgtaa taatacagt gatgatttc 
aattgttgg cacatagct ttaaatttg caacgtgtg 
ttaaactgt agttggcaa aatgggaat tttcagcct 
ttgcataaa ttattaata aacaacaag tgcgtttgg 
taaaataat gtggtcaaa gcaagcgga tatgcgtaa 
cgcaaacat gacatcatt taaaagtaa gataacgaa 
ccggaaaca gaaaaactc aaagtctgt gaatagtca 
gtcggagag tgccgagag tgagagtgc gccgaccgg 
aaaaaacca gcaaaagtc cgggaagca tcgcaatcg 
cggcggtaa aaaagcacc accgagtgc agaaaccac 
tgttacgcc accaagtga catcattgc aagcaccga 
cctcgagct cgcagatgt tgtgctaag gacaagcgt 
tccgagcga agtcgccga acagaacct taaaaatag 
caatcaaaa gaggttcag cgacggctt tgtcattga 
aaatggggc aaacgtgcg tttgacgcg agcggtgag 
cggaattct cggacccga agaattgta caccggcga 
taaaacagc aaagaaagg gagacgctt gagtgatct 
gtttagaca cggtacgct taaagccac aaatagcct 
agaaaactt aaatatgaa acattcgga tcatttgat 
taaagaacc gctgaacaa agcgaccgc agattcgaa 
gaccaagcg gcataaaaa gggctcaag ctacccgct 
ggcgaataa taaagaagc aaagaacgc caaaaattc 
ggaggcggc gcaagccac caaacaaaa ctccgagaa 
caattcgta cttaaaacg agtcaagtc aaagattta 
caaaactaa atccgaaac taacgcttt actaccgtc 
caaaacaac cgtctcgaa gaaaagctc gcaatgtga 
acgcatccc cgaagacgt cgagctgcg aaagtcaaa 
tttcgactt ccgcctgat tcggcaatc ctccaactt 
ggagcaagt ccggagccg aacgttcgg cctcatgaa 
gtcatcttc caataccgg catcgctga tatggaaga 
tggcattgc gaaaaagcc actccttgc caaagcaaa 
gtcaagtag ttccataaa tctcaacct tttgaggat 
gcggcgcag ccgtatact tgaccagtt gcaggaagg 
ccggtgtaa agacgtttt tgatgagtt tgattttat 
tgcattcgg cgggtttag ggataagtt aggcggatc 
gcaaaaact agctacagc agacgcgag gatcgatac 
tgacttagg agaaaataa gcccagtca acccgtgtc 
acgaagcgg atccgaccg aaggccgga tatagtacg 
tgaagagac acccaaaaa tgctgaagg gacgaccag 
caccgacca tggccccaa taagcagga aaacgaaac 
ctccgcccc tgacaatgt cgctagttt cccgcaagc 
ctctcataa gaagaagtc tagcagtaa cataaaccg 
atcggaaag cgtgaaaga gcagtccga aaagcggaa 
acaaagacg gaacattag cgatccctc tcacaatac 
gacaagtgg cgatgtgga gttggatca gccaggctt 
gtcaaatac agattccgg tagttcatc caattctag 
catatccta caatggccg tttgtcaga gagtcctag 
caatagtgt ggacgtcgt caccaccga tggaggcga 
caggaagcc cagttcaga ctgggaaag tgatgccga 
aatggaacc cgccaattg gcagaaatt gttcccaga 
agaagaact acgtaacct ccagccaca cgaaaagaa 
aaggcaaga cgtaattaa cgaactgtt tcacacgga 
gagttccca tgtacggaa ccttcgcgt actgtgtga 
gatttttta caagactct taaggaaag tcaggtttt 
aaagccgga cgaaatagc tttaatttt tccaaatat 
caaagaaat gctcgatgt gcatacgca gttcaatag 
agagatgaa gaggatacg acgggagga tcccttagt 
gagcaatct cggtgacat gttagtcaa aatgtttgg 
cggtgttgt gggcgatat gcttaagcg agccgccgc 
agttttctg cgaacgcca gcagcaagc tctcgaatt 
tatcaagga acggcgtaa acgcgactc gaaatttga 
tggggtttt agtcgagtg cgagaaaaa gcggcaatg 
taggcgatt accactcca agggatcat cccaactga 
aatgcaacg actgtctaa atatcccct tttattgga 
gcggctgat tagcagcgt cgaggccaa cacttcgag 
ctcacatgc gcaggagga gttagtgaa actgaaacg 
tgcgcacca cttttccaa agacatttt aaaccacgt 
taacgaagc tgctaaagt ggcctttaa caaatcacg 
acttgacga aatacagcg gcatctaga cacgacgaa 
ttttgaacg gtcggatca tccaatagc gcaagagtt 
taggatgtt agatctaac gcagtacaa actaatcag 
agaaggtaa tatgcacct acgaagagg gaacaaacc 
aatggtgcc tgttcacgt gctgttact agaagaagc 
tgtcgttat tttacataa agaaggcga taagttctt 
cctaaagtt cttccaatc aggttcctc ggcacagcc 
tcaacccct ctcacctct tataaagat gtccacatt 
gttagtacg gaataatgc ggcctgtaa aagagctct 
cttcctatt caatacttc cacaaacgg cactcaaat 
gtacgatct cctagcaga cgacgatat taatagaga 
cgtgtggct gaaacattt ctcggacgc agccgatgc 
gtataatcg gagagaagg gaaaacgaa aaggcatga 
gtcgcagcc tgactcaga cgagtcgga aactttgca 
ggaaattcc cagtacgcg agatgttgc cgagagggc 
tgaagccgt cggtggaac ggataatac tcctgatga 
tgaagagga ggacgaaca aatcttcag tgatgctca 
ggaggaagg atctacgga gcagaagga ggttccgag 
ctcgtcgga aggtggcgg tgttcacac caaagtaac 
agcggatga gtggcctct catccagcc ttcacaggt 
ccacgtaga agtaccacc agtgcacac tgctgagtc 
aaggttaac tccactaga acaaattcg tcgtaaaga 
tgaagaagt gcgccgagc gttagaaga caaagaggg 
cctggttgc cgacctttt gagtattcc tcgggagca 
cttccaact tattgctga tatggccag tgatacaca 
agacacgcg gtcttccaa agacctagc ggagcgcgt 
attggctgc cgtatatca agtggatca gctgcacct 
agccgtcaa tgaagcgct gaatgtgac ggaggcttc 
agctgtatc agcgacagg tggcaaaaa cccagcttg 
ccaaagcca ggaattgtt gccaaccgt ttcaccacc 
ttccattcc tgccaatcg tgttagaga cgttgccaa 
ctcgctgag ttcacagct aacgaccct acttagtga 
tgtgaaaca agttgagga ggagcgcga tcggcttag 
gaaggagct cttccgcat gagggagcg actgcatga 
gcagcagat gcttcacgg gcctgcaga aaacaacgt 
agaatcgcc tacaccaga actcccacc agacttgga 
cagtattaa ggaaagtcc agccgagcc agagagcta 
acgtgataa gaacactca atatagggc cttgaatat 
cgctaaaca gacaatttg tataaagaa ggaattata 
tttgttata tgctaattt tattattat tatctttta 
ggtaatctt gtgaaagag cctaggtat atttcttat 
tcatgttat ttttataaa taactgaag gaggtgtgt 
tttgatacg gcatgtcct tctgagtta atgtgaaca 
tgatatacg tttttctcc cttcttttt aatgggtgc 
aataatgct ttcactgtt gttcattat tataagtcg 
ctagtactc aatttgaca aggcctaag acggtctaa 
aaattattt aaatgaatg tatattaac aataagtat 
tacgctgtc tacaaagtc attttgttt gtagctatt 
aatttgtac actacgagt ttacacaat tgttatctt 
cagtgtcta ggcggtttt ccaagttga gaaggaacg 
gctggttaa ctactccac ttcaatagt tataaacaa 
tttgattcg tgtaagagt tacgatgta tgttttgtt 
tttatatgt atgttgtct actttgtta cctcatttt 
acaatgtta tttaatgag ataatcaca acttgttaa 
atgttgtct agctttttt ataaccgct ttttaaaaa 
tgcattttc gttataaga tcgcattta tagatgtaa 
agtgtatca ttttttact ttattgtat ctacatatt 
tatagcgaa ttatataat aatgaatga catcgcaat 
tattaaaaa cttgttaag tacacatat ttatataaa 
aaataataa tgctacata ataaactat ttaaaacat 
c
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