Status
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Gene information
General information
TC003064 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 2862583..2867070
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003064
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00508

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003064 RA
      MAPGTQARL ITEWPDLSL GEVVTIIRY DTSQGYLVH 
      TQTHSEELW LPAHALSNY NRKPWPFRF KKPAIIPEL 
      APPEFRDSL RDVTVQSGA KIVLKCRVK NCGRSPKIS 
      WKKLEPNMC VLRNGRFML GEEDEGVAM LIIDNVKLS 
      DSGTYSVTI SNEGGICQS SALLTVTDP YPALQEPQI 
      QVLSCSSVL LEWESEFYH QFWIEYCQL GTGEWLSPN 
      NNRVINSQR YTVENLIPG ETYSFRIIA VQNKLVSLP 
      SVAITLPVA DNLKWQQEQ FKNRYLELE EISRGRFSI 
      VRRAKDRGT NLEVALKQV TRRRQSHHV TQAEYGLLA 
      GMQHVNVIR AMALFDNAP QPGIDTIVL ELVRGPLLF 
      TYLCEKEEY TEATVKTYS RQLMSALLW LHQKDISHL 
      DIKPENVMV DLSGSSPLL KLVDFGDSV NTSKSVILP 
      PACLEFASP ELVLGQPVG KHTDCWAAG VFLYVLLSG 
      VSPFLDDSM EETTANILK CDFCFPDDY FAEISPEAK 
      SLVNNLLVL VPSQRINME ECLKSTWMT TDQKTVVIP 
      SSKLKTFML RRHPMNLPT TPTSPAYFN NEQNVCKT
    CDS sequence
    • >TC003064 RA
      atggccccc ggcacccaa gcccgccta ataaccgaa 
      tggcccgac ttatccctc ggcgaggtc gttaccata 
      atccgctac gacacaagc caaggctac ttggtccac 
      actcagacc cactctgag gagctgtgg ctcccagcc 
      cacgcccta tccaactac aaccgcaaa ccttggccc 
      ttccgcttc aaaaaaccc gccatcatc cccgaactc 
      gcgcccccg gaattccgg gactcgctc cgggacgtc 
      accgtgcaa agcggcgcc aagatcgtg ctgaaatgc 
      cgcgtgaaa aactgcggc cgaagcccc aagatcagc 
      tggaagaag ctcgagccc aacatgtgc gtgctacga 
      aacggacgc ttcatgctg ggtgaagag gacgaaggc 
      gtcgccatg ctgatcata gacaacgtg aagttatca 
      gactcaggg acgtattcg gtcacaatt tcaaacgaa 
      ggtggaata tgtcaaagc tcggcgctt ttgaccgtc 
      acggacccc tatccagcc ctgcaggag ccccagatc 
      caggtcctg tcctgctcc agcgtgctg ctggagtgg 
      gagagcgag ttttatcac caattctgg atcgagtat 
      tgccagttg ggcactggc gaatggctc tccccgaat 
      aataaccgt gtaatcaat tcccagagg tacacggtg 
      gaaaatttg attcctggc gaaacgtac agtttccgg 
      ataattgcc gtgcaaaat aaattagtt agtctgccc 
      agtgttgcc attacgttg ccggtcgct gataatctc 
      aaatggcag caggagcag ttcaagaac aggtatttg 
      gagttggag gagattagc agggggcga ttctccatt 
      gtgcggaga gccaaggac agggggacg aacctggag 
      gtcgccctc aagcaggtc accagacgg aggcaatcg 
      catcatgtc acccaggct gaatacgga ctgcttgca 
      ggaatgcag catgttaac gttattcgg gcaatggcg 
      ttgtttgat aacgcgcca cagcccgga attgataca 
      attgtgtta gaactagtc agagggccg ttactattt 
      acctatctt tgtgagaag gaagagtac acggaggca 
      acagtaaaa acctacagt cggcagtta atgtccgct 
      ctattgtgg ctccatcag aaagatatc tcacacttg 
      gatattaag cctgagaac gtgatggtg gacttgtcg 
      ggctcttcg ccgctcttg aaactggtg gatttcggt 
      gactcggta aacacttcg aagagcgtc attttgcca 
      ccggcttgc ctcgagttc gcctctccc gaacttgtc 
      ttaggtcag ccagttgga aaacacaca gactgttgg 
      gccgctggc gttttcctc tatgtccta ctcagcgga 
      gtttcccct tttctggat gactcaatg gaagagaca 
      actgcgaac atcctcaaa tgcgacttc tgttttccc 
      gatgattac tttgcggaa atttcaccc gaggccaaa 
      agcctcgtg aataacctc ctggtgttg gtcccgtca 
      cagcggata aatatggaa gagtgtctt aaatccacc 
      tggatgacg acggaccag aaaaccgtg gttattccc 
      tcaagtaaa ctgaaaaca tttatgctt cgtagacat 
      cccatgaat ttacctacg acacccact agtccagct 
      tactttaat aatgaacaa aacgtgtgc aagacttaa
    mRNA sequence
    • >TC003064 RA
      tgcgacact cacgtcatg gcccccggc acccaagcc 
      cgcctaata accgaatgg cccgactta tccctcggc 
      gaggtcgtt accataatc cgctacgac acaagccaa 
      ggctacttg gtccacact cagacccac tctgaggag 
      ctgtggctc ccagcccac gccctatcc aactacaac 
      cgcaaacct tggcccttc cgcttcaaa aaacccgcc 
      atcatcccc gaactcgcg cccccggaa ttccgggac 
      tcgctccgg gacgtcacc gtgcaaagc ggcgccaag 
      atcgtgctg aaatgccgc gtgaaaaac tgcggccga 
      agccccaag atcagctgg aagaagctc gagcccaac 
      atgtgcgtg ctacgaaac ggacgcttc atgctgggt 
      gaagaggac gaaggcgtc gccatgctg atcatagac 
      aacgtgaag ttatcagac tcagggacg tattcggtc 
      acaatttca aacgaaggt ggaatatgt caaagctcg 
      gcgcttttg accgtcacg gacccctat ccagccctg 
      caggagccc cagatccag gtcctgtcc tgctccagc 
      gtgctgctg gagtgggag agcgagttt tatcaccaa 
      ttctggatc gagtattgc cagttgggc actggcgaa 
      tggctctcc ccgaataat aaccgtgta atcaattcc 
      cagaggtac acggtggaa aatttgatt cctggcgaa 
      acgtacagt ttccggata attgccgtg caaaataaa 
      ttagttagt ctgcccagt gttgccatt acgttgccg 
      gtcgctgat aatctcaaa tggcagcag gagcagttc 
      aagaacagg tatttggag ttggaggag attagcagg 
      gggcgattc tccattgtg cggagagcc aaggacagg 
      gggacgaac ctggaggtc gccctcaag caggtcacc 
      agacggagg caatcgcat catgtcacc caggctgaa 
      tacggactg cttgcagga atgcagcat gttaacgtt 
      attcgggca atggcgttg tttgataac gcgccacag 
      cccggaatt gatacaatt gtgttagaa ctagtcaga 
      gggccgtta ctatttacc tatctttgt gagaaggaa 
      gagtacacg gaggcaaca gtaaaaacc tacagtcgg 
      cagttaatg tccgctcta ttgtggctc catcagaaa 
      gatatctca cacttggat attaagcct gagaacgtg 
      atggtggac ttgtcgggc tcttcgccg ctcttgaaa 
      ctggtggat ttcggtgac tcggtaaac acttcgaag 
      agcgtcatt ttgccaccg gcttgcctc gagttcgcc 
      tctcccgaa cttgtctta ggtcagcca gttggaaaa 
      cacacagac tgttgggcc gctggcgtt ttcctctat 
      gtcctactc agcggagtt tcccctttt ctggatgac 
      tcaatggaa gagacaact gcgaacatc ctcaaatgc 
      gacttctgt tttcccgat gattacttt gcggaaatt 
      tcacccgag gccaaaagc ctcgtgaat aacctcctg 
      gtgttggtc ccgtcacag cggataaat atggaagag 
      tgtcttaaa tccacctgg atgacgacg gaccagaaa 
      accgtggtt attccctca agtaaactg aaaacattt 
      atgcttcgt agacatccc atgaattta cctacgaca 
      cccactagt ccagcttac tttaataat gaacaaaac 
      gtgtgcaag acttaaagg cacggtttt agcgtgaca 
      tggaccgtt acggaatgt gatataatt gttttatta 
      gtgttttag ttgctatta attactcgc gacacttct 
      tttaaacgt tacgttttc atttttact tttctcgtt 
      tacatcatt ttcaagtgt gattgttaa attattgac 
      gcaagttgt aaataatta cgatacttt aacccatat 
      attgttact gctattata agtattgca gatttgtaa 
      ataaagaga ctatgctgt t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY