Status
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Gene information
General information
TC003035 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 4053515..4057468
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003035
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00506

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003035 RA
      MTETPIIGI LSQEAYLVK DAYPDADSF IVASYIKIL 
      ESSGARVLP IWIGQDAAY YERVVNYTN GILFPGGGT 
      YFNETGGYG EAAKQLYEL AVKTNEKGV HYPLWGICL 
      GMQVLMYGA VGRDIRGNC QSKDVALHL EFVAGYEES 
      KLFSKAPSQ LLENLKTKN LTYNYHRYC IFEDVLKNN 
      NLLTDWRII SKNKDTNGL EFVSSMEHV NYPFFGTQF 
      HPEKNPFEF KKTAVPHCP EAVELAQYF GNFFVDEAR 
      KNNHTFGDK KVEEASLIY NFNPVYSIY NTSYEQLYL 
      FYKQDYNRN LL
    • >TC003035 RB
      MFQYLLILS LTSYINAAE TPIIGILSQ EAYLVKDAY 
      PDADSFIVA SYIKILESS GARVLPIWI GQDAAYYER 
      VVNYTNGIL FPGGGTYFN ETGGYGEAA KQLYELAVK 
      TNEKGVHYP LWGICLGMQ VLMYGAVGR DIRGNCQSK 
      DVALHLEFV AGYEESKLF SKAPSQLLE NLKTKNLTY 
      NYHRYCIFE DVLKNNNLL TDWRIISKN KDTNGLEFV 
      SSMEHVNYP FFGTQFHPE KNPFEFKKT AVPHCPEAV 
      ELAQYFGNF FVDEARKNN HTFGDKKVE EASLIYNFN 
      PVYSIYNTS YEQLYLFYK QDYNRNLL
    CDS sequence
    • >TC003035 RA
      atgactgag actcccatc attgggatt ttatcccaa 
      gaagcttat ttagttaag gatgcttat ccagacgcc 
      gacagcttc atcgtagct tcttacata aaaatcctc 
      gaaagtagc ggtgctcga gttttgccc atttggatc 
      ggccaagat gcggcttat tacgaacgg gtggtgaat 
      tataccaac ggcattcta tttcccggc ggagggacc 
      tactttaat gagacggga ggttatggc gaggcagca 
      aaacagctt tatgaatta gccgtcaaa actaacgag 
      aagggagtt cattaccct ctgtggggc atatgtttg 
      ggtatgcag gtgctgatg tatggggcg gtgggcagg 
      gacatccga ggcaattgc caatcgaag gacgtagct 
      ttgcatctg gagtttgtc gcaggttat gaggagagt 
      aagttattt tccaaagcg ccgtcccag ctgctcgaa 
      aacttgaaa accaaaaac ttgacctac aactaccac 
      cgctactgt atctttgaa gatgtttta aaaaataac 
      aatttatta acggactgg cgaatcatt tcaaaaaat 
      aaggacacc aatggcttg gaatttgtt tcgtcgatg 
      gaacatgtg aattatcca ttctttgga acgcagttt 
      catccggag aaaaacccc ttcgaattt aagaaaacg 
      gcagtccct cactgccca gaagcggtg gaacttgcc 
      cagtatttt ggaaacttt tttgtcgat gaagctagg 
      aaaaacaac cacacattt ggggacaaa aaagtggag 
      gaggcttcg ctgatttac aattttaat cccgtgtac 
      agtatctac aacacgtct tatgagcaa ctgtatttg 
      ttttataag caagactat aacagaaac ttgttgtag
    • >TC003035 RB
      atgttccaa tatctatta attctctct ctcacgagt 
      tacataaat gctgcagag actcccatc attgggatt 
      ttatcccaa gaagcttat ttagttaag gatgcttat 
      ccagacgcc gacagcttc atcgtagct tcttacata 
      aaaatcctc gaaagtagc ggtgctcga gttttgccc 
      atttggatc ggccaagat gcggcttat tacgaacgg 
      gtggtgaat tataccaac ggcattcta tttcccggc 
      ggagggacc tactttaat gagacggga ggttatggc 
      gaggcagca aaacagctt tatgaatta gccgtcaaa 
      actaacgag aagggagtt cattaccct ctgtggggc 
      atatgtttg ggtatgcag gtgctgatg tatggggcg 
      gtgggcagg gacatccga ggcaattgc caatcgaag 
      gacgtagct ttgcatctg gagtttgtc gcaggttat 
      gaggagagt aagttattt tccaaagcg ccgtcccag 
      ctgctcgaa aacttgaaa accaaaaac ttgacctac 
      aactaccac cgctactgt atctttgaa gatgtttta 
      aaaaataac aatttatta acggactgg cgaatcatt 
      tcaaaaaat aaggacacc aatggcttg gaatttgtt 
      tcgtcgatg gaacatgtg aattatcca ttctttgga 
      acgcagttt catccggag aaaaacccc ttcgaattt 
      aagaaaacg gcagtccct cactgccca gaagcggtg 
      gaacttgcc cagtatttt ggaaacttt tttgtcgat 
      gaagctagg aaaaacaac cacacattt ggggacaaa 
      aaagtggag gaggcttcg ctgatttac aattttaat 
      cccgtgtac agtatctac aacacgtct tatgagcaa 
      ctgtatttg ttttataag caagactat aacagaaac 
      ttgttgtag
    mRNA sequence
    • >TC003035 RA
      agttaattc aagaattta atcacaaac gagagacat 
      attacataa tttatttgg gtaatatgt caagagtaa 
      ttttcttaa aattctggc gtcagaata tttattgtt 
      tgctgcaaa tttgagcta tcataaata tgactgaga 
      ctcccatca ttgggattt tatcccaag aagcttatt 
      tagttaagg atgcttatc cagacgccg acagcttca 
      tcgtagctt cttacataa aaatcctcg aaagtagcg 
      gtgctcgag ttttgccca tttggatcg gccaagatg 
      cggcttatt acgaacggg tggtgaatt ataccaacg 
      gcattctat ttcccggcg gagggacct actttaatg 
      agacgggag gttatggcg aggcagcaa aacagcttt 
      atgaattag ccgtcaaaa ctaacgaga agggagttc 
      attaccctc tgtggggca tatgtttgg gtatgcagg 
      tgctgatgt atggggcgg tgggcaggg acatccgag 
      gcaattgcc aatcgaagg acgtagctt tgcatctgg 
      agtttgtcg caggttatg aggagagta agttatttt 
      ccaaagcgc cgtcccagc tgctcgaaa acttgaaaa 
      ccaaaaact tgacctaca actaccacc gctactgta 
      tctttgaag atgttttaa aaaataaca atttattaa 
      cggactggc gaatcattt caaaaaata aggacacca 
      atggcttgg aatttgttt cgtcgatgg aacatgtga 
      attatccat tctttggaa cgcagtttc atccggaga 
      aaaacccct tcgaattta agaaaacgg cagtccctc 
      actgcccag aagcggtgg aacttgccc agtattttg 
      gaaactttt ttgtcgatg aagctagga aaaacaacc 
      acacatttg gggacaaaa aagtggagg aggcttcgc 
      tgatttaca attttaatc ccgtgtaca gtatctaca 
      acacgtctt atgagcaac tgtatttgt tttataagc 
      aagactata acagaaact tgttgtaga ctgattgat 
      tttttgtgt tggttttaa ttgttgaaa taaaagact 
      gttgatgta
    • >TC003035 RB
      caatgtcat aatttttgt aaacgtaat cagcaattt 
      gcccaaaat cataacata atgttcgaa ggtgcgtaa 
      atgcaacgt attactttg atttttcga aggataagc 
      tgtctcagc tgttttgta aacgcgata tgttagtag 
      acgatagac tttcaccat gttccaata tctattaat 
      tctctctct cacgagtta cataaatgc tgcagagac 
      tcccatcat tgggatttt atcccaaga agcttattt 
      agttaagga tgcttatcc agacgccga cagcttcat 
      cgtagcttc ttacataaa aatcctcga aagtagcgg 
      tgctcgagt tttgcccat ttggatcgg ccaagatgc 
      ggcttatta cgaacgggt ggtgaatta taccaacgg 
      cattctatt tcccggcgg agggaccta ctttaatga 
      gacgggagg ttatggcga ggcagcaaa acagcttta 
      tgaattagc cgtcaaaac taacgagaa gggagttca 
      ttaccctct gtggggcat atgtttggg tatgcaggt 
      gctgatgta tggggcggt gggcaggga catccgagg 
      caattgcca atcgaagga cgtagcttt gcatctgga 
      gtttgtcgc aggttatga ggagagtaa gttattttc 
      caaagcgcc gtcccagct gctcgaaaa cttgaaaac 
      caaaaactt gacctacaa ctaccaccg ctactgtat 
      ctttgaaga tgttttaaa aaataacaa tttattaac 
      ggactggcg aatcatttc aaaaaataa ggacaccaa 
      tggcttgga atttgtttc gtcgatgga acatgtgaa 
      ttatccatt ctttggaac gcagtttca tccggagaa 
      aaacccctt cgaatttaa gaaaacggc agtccctca 
      ctgcccaga agcggtgga acttgccca gtattttgg 
      aaacttttt tgtcgatga agctaggaa aaacaacca 
      cacatttgg ggacaaaaa agtggagga ggcttcgct 
      gatttacaa ttttaatcc cgtgtacag tatctacaa 
      cacgtctta tgagcaact gtatttgtt ttataagca 
      agactataa cagaaactt gttgtagac tgattgatt 
      ttttgtgtt ggttttaat tgttgaaat aaaagactg 
      ttgatgta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY