Status
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Gene information
General information
TC003025 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 4223229..4230962
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003025
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00504

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    remarks: animals lost, adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Adult morphology
    remarks: animals lost,
    Fertility
    mature egg deposition 3 number 2nd lateral oviduct: 5 number 1st lateral oviduct: blocked - Number of animals showing the phenotype: 4
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    remarks: animals lost,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 6 (16 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 5 (19 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 5 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003025 RA
      MPESKLQNG RTKSIRVWC DGCYDMVHF GHANSLRQA 
      KALGDYLIV GVHTDEEIT KHKGPPVFN QEERYKMVR 
      GIKWVDEVV EGAPYVTTL ETLDKYDCD FCCHGDDIT 
      MTADGVDTY HLVKAAGRY REVQRTAGV STTDLVGRM 
      LLLTRNHFR QGEREYEVE KEHSSTMGQ DSLARSPWT 
      GCSQFLPTT QKIIQFSDG KPPKPTDKV VYVAGAFDL 
      FHVGHLDFL EKAKQQGDY LIVGLHTDP VVNRYKGSN 
      YPIMNLHER VLSVLACKY VSEVVIGAP YTVTKDLME 
      HFNIDMVVH GCTPVMNDV DGRDPFAYP KQVKKFITV 
      DSGSDMTTE KIVDRIIRN RLEFEARNF KKEKKELEL 
      IKFLEKDKV NGDLINVGN
    CDS sequence
    • >TC003025 RA
      atgcctgaa agtaaatta caaaacggc cggacgaag 
      tcaatccgg gtttggtgt gatgggtgc tacgacatg 
      gttcacttt ggtcacgcc aattcgctc cgacaggcg 
      aaagctttg ggcgattac ctaatagtt ggtgtccac 
      accgatgag gaaatcaca aaacataaa ggacctcct 
      gtattcaac caagaggaa cgatacaaa atggtcagg 
      ggcataaaa tgggtagat gaagtcgta gaaggggcg 
      ccctatgtc acaactttg gaaactttg gacaaatac 
      gactgcgac ttttgctgc cacggggac gatattact 
      atgaccgct gatggagtc gatacttac catttagtt 
      aaagctgca ggacgatat agagaagtc caacggact 
      gccggcgtc tccaccacc gacctcgtg ggccgcatg 
      ctcctcctc acccgcaac cacttccgg caaggcgaa 
      cgcgaatac gaagtcgag aaggaacat tcctccaca 
      atgggccag gactccctc gcgaggtcg ccctggacc 
      ggctgttcc caattctta cccaccacc cagaaaata 
      atccagttt tcggacgga aaacccccc aaaccaacc 
      gataaggtt gtttacgtg gccggcgct tttgatctc 
      ttccacgtg ggtcatctc gatttcttg gaaaaagcc 
      aaacaacaa ggcgattac ctcatcgtg gggctgcac 
      accgacccg gtggtcaac cggtacaaa ggctcgaat 
      taccccatt atgaacctg cacgagcgg gttctgagc 
      gtgctggcg tgcaagtac gtctcggag gtggtcatt 
      ggggcgccc tataccgtt accaaagat ttgatggag 
      cattttaac atcgatatg gtcgttcat gggtgtacc 
      ccagttatg aacgacgtc gatgggcga gatcccttc 
      gcgtacccg aaacaagtg aagaagttt atcaccgtt 
      gattcaggg agtgacatg acgacggag aaaattgtc 
      gatcgaata atcaggaac aggctggag tttgaggcg 
      agaaatttc aagaaggag aagaaggag ttggaattg 
      atcaagttt ttggagaaa gataaagtg aacggtgat 
      ctaatcaat gtcggcaat taa
    mRNA sequence
    • >TC003025 RA
      gctgagaaa aaccattta tagatagat tctacaaat 
      aaatcaatc ggtgtttac agttaattg atggaaatt 
      gtggaaatc atcttacaa gctgttaag taatctccc 
      aaacttaaa cagtttaaa ataaaatgt agaaaaact 
      aacacatct ggaaaatag ggcacgtga aaattgttt 
      ttgtacttt tattccgtt gcaataata aactgtttc 
      acgcctact ccaagatga attttggga gattaaaaa 
      acacaagca ctcattgac tcatcttac acgccatca 
      gtcgagaac attgcaaaa ttatgaaca ctctagtga 
      taaaaatga tattccaat gaacttgga aaagttgtt 
      atggaacat attaaaatt tgaaatact tattgtagg 
      tgcatacat aatacttat ttacaaaca aaactccat 
      gaatgttta cgccaaaaa ctggagacg gtctaaacg 
      atcactttt ggtgatcgg gcggtgaac attgttgga 
      caatttgca tttgtttca tgttaatat gcgatagat 
      acgatcata tttatacag tgtgctttt atacaaata 
      ccattttac ttgtaacgt gtatgtctt tgtattgtc 
      atattcata tctacataa atcgatgta taaaattaa 
      ttttgatac aagttatca gtgatacct aatcggctt 
      tcgtgtgtt taagtttgg tcatctgtg tatactaat 
      tgcttcctt taggtttca tcatccact tggctttgt 
      aacgttaga tgaaatctt aaatagggt gatcttcca 
      cgtgcgatt ttgcaaaga gaggtttga cggtgttgt 
      gagccccgt tttgttacc aaattaaat ctatacagg 
      gtgtcgcca atgtaagct tgaaactgt acaattctt 
      ttaatattt gttacacat ttctaaggg cacgctgat 
      aagatctgg agttggcat ttaaaaaat ccaataaaa 
      aataaaact attaactat taacaatag gtgcgctgt 
      tgtaataac gcatttaaa aaatccaat tacggttgc 
      aatttatga aaaataaat taatttgat cattgttta 
      aaactgtta acaataggt gcgctgttg taacaaccc 
      ataaatcaa atggaaaaa taatttgtt tttccaaag 
      atgtgcctt tgtactttg agctggtta gccggttcg 
      aatgtcaaa attttcaac atttagggg cgtaatagc 
      aaatactaa ccacagttt tctgtaata attgtgcca 
      acagtcaca ccatcgctc atatttcaa acatatcac 
      gaaacatct catcacttt ctcttctca gccttatag 
      aactgtgtc tcaaatatc cgtttaatt tcggctttg 
      atctctcaa cctgctacc aagcgttgt aaattaaga 
      acaagtgct taattctaa tagaattcg gaaaaattt 
      tatattaaa tttttatat gataggtat tttaataat 
      ggttgtgag cattacctg acaatttta attctctta 
      gaattaagg ctacgtacc aagttagat taacattgg 
      ttagtccat tcagcttaa ttattaccg catagatat 
      ttttgtatc atctcgaat acacttcaa ttgttcctt 
      tttgtgttt tgttggatg agttgcttg ccatatagc 
      aaatggtgg ggatgaaga cgtggctga acgtggtgt 
      gacaaatct aaagggcat cggaacacc ccacctcta 
      caatcaatt acgtttctt ggtcattgc tggctgatt 
      cggttctgt tattctcat tcattatgt gaaaattgt 
      ttgggtagg gagttgcta aacttcgag gagagcgct 
      cacacacga gttattttt agtcaggaa attgactgt 
      ggtgaatga aagtttagc cttctatcg gtttatgtg 
      cttttaaga gaattgggg gaaatatgc ctgaaagta 
      aattacaaa acggccgga cgaagtcaa tccgggttt 
      ggtgtgatg ggtgctacg acatggttc actttggtc 
      acgccaatt cgctccgac aggcgaaag ctttgggcg 
      attacctaa tagttggtg tccacaccg atgaggaaa 
      tcacaaaac ataaaggac ctcctgtat tcaaccaag 
      aggaacgat acaaaatgg tcaggggca taaaatggg 
      tagatgaag tcgtagaag gggcgccct atgtcacaa 
      ctttggaaa ctttggaca aatacgact gcgactttt 
      gctgccacg gggacgata ttactatga ccgctgatg 
      gagtcgata cttaccatt tagttaaag ctgcaggac 
      gatatagag aagtccaac ggactgccg gcgtctcca 
      ccaccgacc tcgtgggcc gcatgctcc tcctcaccc 
      gcaaccact tccggcaag gcgaacgcg aatacgaag 
      tcgagaagg aacattcct ccacaatgg gccaggact 
      ccctcgcga ggtcgccct ggaccggct gttcccaat 
      tcttaccca ccacccaga aaataatcc agttttcgg 
      acggaaaac cccccaaac caaccgata aggttgttt 
      acgtggccg gcgcttttg atctcttcc acgtgggtc 
      atctcgatt tcttggaaa aagccaaac aacaaggcg 
      attacctca tcgtggggc tgcacaccg acccggtgg 
      tcaaccggt acaaaggct cgaattacc ccattatga 
      acctgcacg agcgggttc tgagcgtgc tggcgtgca 
      agtacgtct cggaggtgg tcattgggg cgccctata 
      ccgttacca aagatttga tggagcatt ttaacatcg 
      atatggtcg ttcatgggt gtaccccag ttatgaacg 
      acgtcgatg ggcgagatc ccttcgcgt acccgaaac 
      aagtgaaga agtttatca ccgttgatt cagggagtg 
      acatgacga cggagaaaa ttgtcgatc gaataatca 
      ggaacaggc tggagtttg aggcgagaa atttcaaga 
      aggagaaga aggagttgg aattgatca agtttttgg 
      agaaagata aagtgaacg gtgatctaa tcaatgtcg 
      gcaattaat ttcaatttt attcgaatt ttattgagt 
      acaaattaa ttcaaaatc aatgctttc ctagtcggc 
      gttttacca aatatttaa aggccagga aacaagttg 
      ggatacgtt tgtgataaa tgttttatt aatttaact 
      ggaacgcgt tgaatcatc acaacaaac cttgactag 
      ttttttgca tgatgtcac tacttggct tcattctgc 
      tttgatcaa ggtaatttt aggtgttgt aacgtattt 
      atcccatct tgtgtttta tttattact ttcttatat 
      ttattatag tggtgcaca caaagaaat tagagggtc 
      actattttc atacaatcg ggattagct tgtctagaa 
      taatcacaa gagacaata ctcgatctc ttaaccgaa 
      cataaagat atgcatact cattatttt atgtggaac 
      gttttgtag attatttgt tcttaaatg gccatttta 
      ttttccatt accttcgtt gtaagtgtt tttccgtac 
      tgtattaaa tattgaata ggtagtttg ataacaaaa 
      tctgagttg ttaaaccgt agtattctg ttttataat 
      cttcactag tgtgttacg ttgtgatat ttatgtaat 
      ggctgtttt tgtacagaa tagaggttc tacattttc 
      cattttgtc ttctatggc atttttaaa ttttaatgt 
      attgactag taagagctt tatatgtta cgtgatcta 
      cacatgctt attttacct catttgtcg agaaaaatt 
      aatgttact gtgaatata ttagccaaa attaccttt 
      attttcttg ttggcaaaa tgttatttg tatataata 
      tatttttct gcaaagc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY