Status
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Gene information
General information
TC003020 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 4326000..4329820
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003020
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00503

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 8 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection


    potentially confused/crosscontaminated with: 0
    Pupal morphology
    potentially confused/crosscontaminated with: 0
    Adult morphology
    potentially confused/crosscontaminated with: 0
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003020 RA
      MVLIAAAVC TKAGKTIVS RQFVEMTKA RIEGLLAAF 
      PKLIPTGTQ HTFVETDSV RYVYQPLER LYMLLITTR 
      ASNILEDLE TLRLFAKVI PEYCRSLEE NEIAENAFS 
      LIFAFDEIV ALGYRESVN LSQIRTFVE MDSHEERVY 
      QAVRQTQER EAKNKMREK AKELQRQRM EANKKGVKP 
      SFGSSGGFG SSSGYTPAP SVGDVANIS NDIKPPAYS 
      SAPQKPSTR GMKLGGKGH DVESFVDQL KSEGENVIA 
      PVNNSISQG GMKTPAIKS DINDVHLRL EEKLIVRMG 
      RDGGVQQFE LLGLVTLHI GDEKWGRIR VQLENKDTR 
      GIQLQTHPN VDKELFKLR SQIGLKQPS KPFPLHTDV 
      GVLKWRLQS TDESLVPLL INCWPSEVG DGSCDINIE 
      YELADPNLE LADVNILIP LPMGCNPVV GECDGSYTH 
      ESRRNQLIW NLPIIDSSN KTGSLEFSA PRSIPNDFF 
      PVTVSFSSK SAYANIKIL DVLMVDDDS PVKYSVETS 
      LFPDKYEIV
    CDS sequence
    • >TC003020 RA
      atggtgctc atagcagcg gcggtctgc actaaggcc 
      ggtaaaacc atagtctct cggcagttt gttgagatg 
      accaaagcc cgcattgag ggtctcctg gcggctttc 
      cccaaactt atcccaaca ggaacacaa cacaccttt 
      gtagagacc gactcggtc cgatatgta tatcagcca 
      cttgagcgc ctttacatg ctcctgatt accacaaga 
      gctagcaac attttggaa gatttagaa actctgagg 
      ttgtttgcg aaagtgatt cccgaatat tgccgctca 
      ttggaagaa aatgaaatt gcggaaaac gcattttcc 
      ctcattttt gcgtttgat gagattgtt gctcttggg 
      tacagggaa agcgtcaat ttgagccaa attcgcaca 
      tttgtcgag atggattca cacgaagag cgggtctat 
      caggccgtc agacagaca caagaacgc gaagctaaa 
      aacaaaatg cgtgagaaa gctaaagaa ttgcaacgc 
      cagcgaatg gaagccaac aaaaagggc gtcaaacca 
      tcatttggt agcagtgga ggctttggt agctcatct 
      ggttatact ccagcccct tcagtcggc gatgttgcc 
      aatatttca aacgatatc aaaccacca gcttattcc 
      tccgctcct cagaaacct tcgactcga ggaatgaaa 
      ctgggtggc aaaggccac gacgttgag tctttcgta 
      gaccagctt aaatccgaa ggcgaaaat gtcattgca 
      ccagttaat aacagtatt tcacaaggg ggaatgaaa 
      acaccagcg atcaagtcc gatattaat gatgttcac 
      ttgagactg gaggaaaaa ctaatcgtt cgaatgggc 
      cgtgacggc ggtgtgcaa caattcgag ttgttgggg 
      ttggtgaca ctgcacatt ggggacgaa aagtggggg 
      cgaattcgt gtccagttg gaaaataaa gacacgcgt 
      ggcatccag ctccaaact caccccaat gtagacaag 
      gaacttttc aaactgcgt tcccaaatt gggctcaaa 
      caaccatcc aaaccattc ccgttgcac acagacgtc 
      ggtgtcctg aaatggcga ttgcaaagc acagatgag 
      agtttagtg cctctcttg atcaactgt tggccgtcg 
      gaagtgggc gatggcagt tgcgacatc aatatcgag 
      tacgaactt gccgatccg aatttagag ctggctgat 
      gtcaatatt ttgattccg ttaccgatg ggttgtaac 
      ccggttgtg ggcgaatgt gatggctca tacacgcat 
      gagtcaaga cgtaaccag ctaatatgg aatctacct 
      attatagat tcaagcaat aaaactgga tcgttggaa 
      tttagtgcg ccccgatct attcctaac gactttttc 
      ccggtgaca gtttctttt agttcgaaa tctgcttac 
      gcaaatatt aagattctt gatgtatta atggtggac 
      gatgattct cctgttaag tattctgtt gaaactagc 
      ttgtttccc gacaaatat gagatagtg taa
    mRNA sequence
    • >TC003020 RA
      tttgatttt aagtaactt atgagtaga aactggagc 
      attttcgaa ttttgcgcc tagagctgg tgcagtgaa 
      tcggcgtaa ggttggctg actgaatac ggtcacgtc 
      actgttttt ctcattcaa cagcaagca caagtcaac 
      gtcatctta aaaaattaa tagggtgta atgttttgt 
      aaaatatta ttttagttt ttaggaaca taacagcaa 
      cagaccttt cacagtctc taaaatggt gctcatagc 
      agcggcggt ctgcactaa ggccggtaa aaccatagt 
      ctctcggca gtttgttga gatgaccaa agcccgcat 
      tgagggtct cctggcggc tttccccaa acttatccc 
      aacaggaac acaacacac ctttgtaga gaccgactc 
      ggtccgata tgtatatca gccacttga gcgccttta 
      catgctcct gattaccac aagagctag caacatttt 
      ggaagattt agaaactct gaggttgtt tgcgaaagt 
      gattcccga atattgccg ctcattgga agaaaatga 
      aattgcgga aaacgcatt ttccctcat ttttgcgtt 
      tgatgagat tgttgctct tgggtacag ggaaagcgt 
      caatttgag ccaaattcg cacatttgt cgagatgga 
      ttcacacga agagcgggt ctatcaggc cgtcagaca 
      gacacaaga acgcgaagc taaaaacaa aatgcgtga 
      gaaagctaa agaattgca acgccagcg aatggaagc 
      caacaaaaa gggcgtcaa accatcatt tggtagcag 
      tggaggctt tggtagctc atctggtta tactccagc 
      cccttcagt cggcgatgt tgccaatat ttcaaacga 
      tatcaaacc accagctta ttcctccgc tcctcagaa 
      accttcgac tcgaggaat gaaactggg tggcaaagg 
      ccacgacgt tgagtcttt cgtagacca gcttaaatc 
      cgaaggcga aaatgtcat tgcaccagt taataacag 
      tatttcaca agggggaat gaaaacacc agcgatcaa 
      gtccgatat taatgatgt tcacttgag actggagga 
      aaaactaat cgttcgaat gggccgtga cggcggtgt 
      gcaacaatt cgagttgtt ggggttggt gacactgca 
      cattgggga cgaaaagtg ggggcgaat tcgtgtcca 
      gttggaaaa taaagacac gcgtggcat ccagctcca 
      aactcaccc caatgtaga caaggaact tttcaaact 
      gcgttccca aattgggct caaacaacc atccaaacc 
      attcccgtt gcacacaga cgtcggtgt cctgaaatg 
      gcgattgca aagcacaga tgagagttt agtgcctct 
      cttgatcaa ctgttggcc gtcggaagt gggcgatgg 
      cagttgcga catcaatat cgagtacga acttgccga 
      tccgaattt agagctggc tgatgtcaa tattttgat 
      tccgttacc gatgggttg taacccggt tgtgggcga 
      atgtgatgg ctcatacac gcatgagtc aagacgtaa 
      ccagctaat atggaatct acctattat agattcaag 
      caataaaac tggatcgtt ggaatttag tgcgccccg 
      atctattcc taacgactt tttcccggt gacagtttc 
      ttttagttc gaaatctgc ttacgcaaa tattaagat 
      tcttgatgt attaatggt ggacgatga ttctcctgt 
      taagtattc tgttgaaac tagcttgtt tcccgacaa 
      atatgagat agtgtaagt gtcacagta ggttacgat 
      agttaatga aattatata ataataaaa ttcacttgt 
      ataaatact actaatatt ttattcgtt cttgctttt 
      tgtacaaaa ttttaagaa gtttgtttt aatttttat 
      ctattatat aaataaaat aagtcagga gta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY