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Gene information
General information
TC032364
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC003019 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 4365581..4372318
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC032364
    iBeetle screen iB_00502

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    TR: 47342 potentially confused/crosscontaminated with : iB # (comma separated) 00486
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032364 RA
      MVLGKGSFG KVMLAERKG TDELYAIKI LKKDIIIQD 
DDVECTMVE KRVLALSNK PPFLVQLHS CFQTMDRLY 
FVMEYVNGG DLMFQIQQC GKFKEPVAV FYSAEIAIG 
LFFLHSRGI VYRDLKLDN VLLDQDGHI KIADFGMCK 
EGITGDKTT KTFCGTPDY IAPEIILYQ PYGKSVDWW 
AYGVLLYEM LVGQPPFDG EDEEELFAA ITDHNVSYP 
KGLSKEAKD VCKGLLTKN PNKRLGCGP HGEEDVRIH 
AFFRRIDWE KIENREVQP PFKPKIKHR KDVSNFDKQ 
FTSEKTDLT PTDKLFMMN LDQTEFMGF SFLNPEFIQ 
HV
    CDS sequence
    • >TC032364 RA
      atggtttta ggcaaaggc tcattcggc aaagtaatg 
ttggctgaa aggaaagga acggacgaa ttgtacgca 
ataaaaatt ttgaaaaag gacataatc atccaagac 
gacgacgtc gaatgcacc atggtggaa aagcgcgtc 
ctggctctc tccaacaaa ccgcctttc ctagtccaa 
ctccattcg tgtttccaa acaatggac cggctgtac 
tttgttatg gaatacgtg aacggcggt gatctcatg 
ttccaaatt cagcaatgt ggcaaattt aaagaacct 
gttgcggtt ttttattcg gctgaaatt gccatcggt 
ttgttcttt ttacacagc cggggtata gtttacagg 
gacctgaaa ctcgataac gttttattg gatcaagat 
ggacacatc aaaattgcc gactttggg atgtgcaag 
gaagggatt acgggcgat aaaactacg aaaactttc 
tgtggtacc cctgactac atcgcaccc gagataatt 
ttgtaccaa ccgtacgga aaatcggtc gattggtgg 
gcttacggt gtcctgctg tacgaaatg ttggtcgga 
cagccccct ttcgacgga gaggacgag gaagagttg 
tttgcagct atcacagat cacaacgtt agctatccg 
aagggtttg tccaaagaa gccaaggat gtgtgcaaa 
gggcttttg accaaaaac ccgaacaaa cggctgggt 
tgcggtccg cacggggag gaagacgtc cgtattcat 
gctttcttt agacgaatc gattgggaa aagattgaa 
aatcgggag gttcagccc cctttcaag ccgaaaatt 
aaacatcgc aaagatgtc agcaatttt gataagcag 
ttcacttcg gaaaaaacg gatctgacg cccaccgat 
aaattgttt atgatgaat ctggatcag acggagttt 
atgggattt tcgttcctc aatccggaa tttatccaa 
catgtttaa
    mRNA sequence
    • >TC032364 RA
      aatattgta tacctacgt ctataaaaa aataaaata 
ttgaatagc agacgctaa atttgtcac agttggaaa 
aagtttaga aaaacgtaa ccgtttttg aagaattat 
tgaataaaa taaaccaaa acatttgac taacaacat 
tttttttag atataccta cgtctataa aaaataaaa 
tattgaata gcagacgct aaatttgtc acagttgga 
aaaaagtga ccgttttta aaagaatta ttgcaataa 
aataagcca aaacatttt tcaacacaa ttttgggaa 
cagtttaca actttgtgg aaaaattgc gttatttgt 
ttttttatt tctaaattt ttgtgtttc attgttcct 
ctaatttga cttcgtaga tctcatttt tatggtatt 
tttaaaggt aaaaattaa tttttggag gaaataatt 
gggtctcca atttagttt ttccaaaaa gtaaattaa 
tatttgttc aaccagaaa acaattcgt tcggtacat 
attttttta ttttgtttt ttgatttct ttgacagat 
tttgttcta aatgtttat tcatagtta atagaaact 
tgattgaaa aattggaag tgagcactg tttctccag 
tgctttgat tgttttttt atttccaaa tttttatgt 
ttgattatt tctccaatg acttcgtag atctcagtt 
ggaggatag gtttgaaag gtaaaaatc gatttttga 
aaggaaaaa tgtagcgaa atatccgaa ttaggtttt 
tcttaaaag taaataaat gtttatttc aatcattcg 
ttcaaaaca tattttttt attttgttt attaatttt 
tttgctaga ttttgttct gatttactt tttttctaa 
atatttata catagttat agaataatg tataatttt 
ttagcagaa aggactttg ctctaacaa atcaaacaa 
ttgaatgtt attggttat tatgataaa cagagagta 
actagaagt ggttataat aaatcagtg gcgatatga 
ctaattctg ataaacaaa taaagtaaa taaacaaat 
aaatccatt gttttccat ttcccaatt ttcacaatt 
actacgtaa acgtaccta cgtaaaaaa gtcgttagt 
caaagggtt atcagagaa aataattta ttcaatttt 
tttacaaaa tcccttttt ttacaaagt taattagcg 
cagtaacag caacatttt gtggcccct ttagttaga 
aaagaacgt tttattttc tccgtgttt tttacgatg 
cccctcgac gtgatatca aaaatacaa atcaagatc 
aaacagctt taatatttc tcaatatat tggctggaa 
tggctattt tatcgccaa tatgaatag taaaatata 
cagtttgat ggtttacag aacaggaaa gacttaaca 
ttaattgtt tataattaa taaattttt aagaaaaac 
gtcaataac caagaagcc gtcgatcac gaccgacaa 
agacgtccc tcacaacat gggcaaaaa gacataatt 
agggcgagc gatttcaat ttcctcatg gttttaggc 
aaaggctca ttcggcaaa gtaatgttg gctgaaagg 
aaaggaacg gacgaattg tacgcaata aaaattttg 
aaaaaggac ataatcatc caagacgac gacgtcgaa 
tgcaccatg gtggaaaag cgcgtcctg gctctctcc 
aacaaaccg cctttccta gtccaactc cattcgtgt 
ttccaaaca atggaccgg ctgtacttt gttatggaa 
tacgtgaac ggcggtgat ctcatgttc caaattcag 
caatgtggc aaatttaaa gaacctgtt gcggttttt 
tattcggct gaaattgcc atcggtttg ttcttttta 
cacagccgg ggtatagtt tacagggac ctgaaactc 
gataacgtt ttattggat caagatgga cacatcaaa 
attgccgac tttgggatg tgcaaggaa gggattacg 
ggcgataaa actacgaaa actttctgt ggtacccct 
gactacatc gcacccgag ataattttg taccaaccg 
tacggaaaa tcggtcgat tggtgggct tacggtgtc 
ctgctgtac gaaatgttg gtcggacag ccccctttc 
gacggagag gacgaggaa gagttgttt gcagctatc 
acagatcac aacgttagc tatccgaag ggtttgtcc 
aaagaagcc aaggatgtg tgcaaaggg cttttgacc 
aaaaacccg aacaaacgg ctgggttgc ggtccgcac 
ggggaggaa gacgtccgt attcatgct ttctttaga 
cgaatcgat tgggaaaag attgaaaat cgggaggtt 
cagccccct ttcaagccg aaaattaaa catcgcaaa 
gatgtcagc aattttgat aagcagttc acttcggaa 
aaaacggat ctgacgccc accgataaa ttgtttatg 
atgaatctg gatcagacg gagtttatg ggattttcg 
ttcctcaat ccggaattt atccaacat gtttaagtg 
tttacgata cgtatctct ttttttaat atttagagt 
agcgtagtt gcgttcgca atttgcatc tcagtcgtt 
cttgagcaa tgtttattt cgatataat cgactattt 
atatatgaa tatactcac ctaaagtat gcatgattt 
gcattcgaa gtagttaat gtatagtgc caaaaaacg 
gaactttct catcccaat gttaagtaa taaaagtgt 
acataaata tattaagta acgtagtgt ttcttgttc 
cactctgaa acatatcaa atattacat accacgacc 
gttgaggag cgcaagagt ggttattta accaaagct 
tgatacgta aggagggtc cagttcccg tagacctag 
tacatagtg aagcatttc gttgataca gttttattt 
tagcgattg tattattat ccccagtat tgaaactca 
ttaagcgac atctgtgac ccctagagg atattgaaa 
ctgtattta taaacttag tcatcgctt tcgcttagt 
gtttctttc gctactagt gatgaattg tgatttttg 
agcaaattg acaatctta agcgaaaca aactaactg 
atattgtaa actaagcat tccacaaat gcagttgag 
acattcctt agttgtagc tttcaaaat gactgactt 
gcaaattgc ctgcttacc acatctcgt ttttactct 
cgacaactg tggcacttt agaaacagt tatattgta 
attactaat ggtaacatt gttgttcac gcgtctttt 
tcttacagt tcttccagt catgacaat gacgtgatt 
tgtgaatgt agttattta taagtatat ttaaaatta 
tcaaatatg tacctattt ctccaagcg gtgtaatct 
tgacgtgag ccacacctc tcaaaagaa atatttttt 
aagattgtg gcagtgtta atattcaaa ttgttccca 
aaacgatag tgtttcaca cagaaagcc actcaactg 
tactactgc ccgctcaag actcctaaa ttgtcagct 
tttctaatc gatctacaa cactaaaca acacaatct 
ggcccggct tttgtgtga aataaccac aaccgaact 
tatttttaa acaatgtcg gttgttcaa gtcaaaaaa 
tcactttgc tgtgtgttg gacaagtta aggaattac 
ttctttgtt ggatttctt aaatgttga tcctgtcta 
aattaattt aagttatta attaatatt gttattaat 
tccgttaca tatagttta tttttgtta ttgcgatat 
ctttcacat tgccatttt gcatccaac cactattac 
gaatttaat aattcttta ctatttttt taaaggaat 
taaaattaa ttgttggag ggttggtgt aaggggcat 
tacgtatct gtaagtgca tgtgacatt ctagtcatt 
tgtaacaaa tgctatcta gtctcataa tgtatggtc 
taagtgatc tttttgata taatctgtt tactgttag 
aatctgata tgtattatt cttgttgta accattact 
tattcatgt attataa
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