| General information | |||
| TC032364
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 4365581..4372318 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC032364 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_00502 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)
- >TC032364 RA
MVLGKGSFG KVMLAERKG TDELYAIKI LKKDIIIQD DDVECTMVE KRVLALSNK PPFLVQLHS CFQTMDRLY FVMEYVNGG DLMFQIQQC GKFKEPVAV FYSAEIAIG LFFLHSRGI VYRDLKLDN VLLDQDGHI KIADFGMCK EGITGDKTT KTFCGTPDY IAPEIILYQ PYGKSVDWW AYGVLLYEM LVGQPPFDG EDEEELFAA ITDHNVSYP KGLSKEAKD VCKGLLTKN PNKRLGCGP HGEEDVRIH AFFRRIDWE KIENREVQP PFKPKIKHR KDVSNFDKQ FTSEKTDLT PTDKLFMMN LDQTEFMGF SFLNPEFIQ HV
- >TC032364 RA
atggtttta ggcaaaggc tcattcggc aaagtaatg ttggctgaa aggaaagga acggacgaa ttgtacgca ataaaaatt ttgaaaaag gacataatc atccaagac gacgacgtc gaatgcacc atggtggaa aagcgcgtc ctggctctc tccaacaaa ccgcctttc ctagtccaa ctccattcg tgtttccaa acaatggac cggctgtac tttgttatg gaatacgtg aacggcggt gatctcatg ttccaaatt cagcaatgt ggcaaattt aaagaacct gttgcggtt ttttattcg gctgaaatt gccatcggt ttgttcttt ttacacagc cggggtata gtttacagg gacctgaaa ctcgataac gttttattg gatcaagat ggacacatc aaaattgcc gactttggg atgtgcaag gaagggatt acgggcgat aaaactacg aaaactttc tgtggtacc cctgactac atcgcaccc gagataatt ttgtaccaa ccgtacgga aaatcggtc gattggtgg gcttacggt gtcctgctg tacgaaatg ttggtcgga cagccccct ttcgacgga gaggacgag gaagagttg tttgcagct atcacagat cacaacgtt agctatccg aagggtttg tccaaagaa gccaaggat gtgtgcaaa gggcttttg accaaaaac ccgaacaaa cggctgggt tgcggtccg cacggggag gaagacgtc cgtattcat gctttcttt agacgaatc gattgggaa aagattgaa aatcgggag gttcagccc cctttcaag ccgaaaatt aaacatcgc aaagatgtc agcaatttt gataagcag ttcacttcg gaaaaaacg gatctgacg cccaccgat aaattgttt atgatgaat ctggatcag acggagttt atgggattt tcgttcctc aatccggaa tttatccaa catgtttaa
- >TC032364 RA
aatattgta tacctacgt ctataaaaa aataaaata ttgaatagc agacgctaa atttgtcac agttggaaa aagtttaga aaaacgtaa ccgtttttg aagaattat tgaataaaa taaaccaaa acatttgac taacaacat tttttttag atataccta cgtctataa aaaataaaa tattgaata gcagacgct aaatttgtc acagttgga aaaaagtga ccgttttta aaagaatta ttgcaataa aataagcca aaacatttt tcaacacaa ttttgggaa cagtttaca actttgtgg aaaaattgc gttatttgt ttttttatt tctaaattt ttgtgtttc attgttcct ctaatttga cttcgtaga tctcatttt tatggtatt tttaaaggt aaaaattaa tttttggag gaaataatt gggtctcca atttagttt ttccaaaaa gtaaattaa tatttgttc aaccagaaa acaattcgt tcggtacat attttttta ttttgtttt ttgatttct ttgacagat tttgttcta aatgtttat tcatagtta atagaaact tgattgaaa aattggaag tgagcactg tttctccag tgctttgat tgttttttt atttccaaa tttttatgt ttgattatt tctccaatg acttcgtag atctcagtt ggaggatag gtttgaaag gtaaaaatc gatttttga aaggaaaaa tgtagcgaa atatccgaa ttaggtttt tcttaaaag taaataaat gtttatttc aatcattcg ttcaaaaca tattttttt attttgttt attaatttt tttgctaga ttttgttct gatttactt tttttctaa atatttata catagttat agaataatg tataatttt ttagcagaa aggactttg ctctaacaa atcaaacaa ttgaatgtt attggttat tatgataaa cagagagta actagaagt ggttataat aaatcagtg gcgatatga ctaattctg ataaacaaa taaagtaaa taaacaaat aaatccatt gttttccat ttcccaatt ttcacaatt actacgtaa acgtaccta cgtaaaaaa gtcgttagt caaagggtt atcagagaa aataattta ttcaatttt tttacaaaa tcccttttt ttacaaagt taattagcg cagtaacag caacatttt gtggcccct ttagttaga aaagaacgt tttattttc tccgtgttt tttacgatg cccctcgac gtgatatca aaaatacaa atcaagatc aaacagctt taatatttc tcaatatat tggctggaa tggctattt tatcgccaa tatgaatag taaaatata cagtttgat ggtttacag aacaggaaa gacttaaca ttaattgtt tataattaa taaattttt aagaaaaac gtcaataac caagaagcc gtcgatcac gaccgacaa agacgtccc tcacaacat gggcaaaaa gacataatt agggcgagc gatttcaat ttcctcatg gttttaggc aaaggctca ttcggcaaa gtaatgttg gctgaaagg aaaggaacg gacgaattg tacgcaata aaaattttg aaaaaggac ataatcatc caagacgac gacgtcgaa tgcaccatg gtggaaaag cgcgtcctg gctctctcc aacaaaccg cctttccta gtccaactc cattcgtgt ttccaaaca atggaccgg ctgtacttt gttatggaa tacgtgaac ggcggtgat ctcatgttc caaattcag caatgtggc aaatttaaa gaacctgtt gcggttttt tattcggct gaaattgcc atcggtttg ttcttttta cacagccgg ggtatagtt tacagggac ctgaaactc gataacgtt ttattggat caagatgga cacatcaaa attgccgac tttgggatg tgcaaggaa gggattacg ggcgataaa actacgaaa actttctgt ggtacccct gactacatc gcacccgag ataattttg taccaaccg tacggaaaa tcggtcgat tggtgggct tacggtgtc ctgctgtac gaaatgttg gtcggacag ccccctttc gacggagag gacgaggaa gagttgttt gcagctatc acagatcac aacgttagc tatccgaag ggtttgtcc aaagaagcc aaggatgtg tgcaaaggg cttttgacc aaaaacccg aacaaacgg ctgggttgc ggtccgcac ggggaggaa gacgtccgt attcatgct ttctttaga cgaatcgat tgggaaaag attgaaaat cgggaggtt cagccccct ttcaagccg aaaattaaa catcgcaaa gatgtcagc aattttgat aagcagttc acttcggaa aaaacggat ctgacgccc accgataaa ttgtttatg atgaatctg gatcagacg gagtttatg ggattttcg ttcctcaat ccggaattt atccaacat gtttaagtg tttacgata cgtatctct ttttttaat atttagagt agcgtagtt gcgttcgca atttgcatc tcagtcgtt cttgagcaa tgtttattt cgatataat cgactattt atatatgaa tatactcac ctaaagtat gcatgattt gcattcgaa gtagttaat gtatagtgc caaaaaacg gaactttct catcccaat gttaagtaa taaaagtgt acataaata tattaagta acgtagtgt ttcttgttc cactctgaa acatatcaa atattacat accacgacc gttgaggag cgcaagagt ggttattta accaaagct tgatacgta aggagggtc cagttcccg tagacctag tacatagtg aagcatttc gttgataca gttttattt tagcgattg tattattat ccccagtat tgaaactca ttaagcgac atctgtgac ccctagagg atattgaaa ctgtattta taaacttag tcatcgctt tcgcttagt gtttctttc gctactagt gatgaattg tgatttttg agcaaattg acaatctta agcgaaaca aactaactg atattgtaa actaagcat tccacaaat gcagttgag acattcctt agttgtagc tttcaaaat gactgactt gcaaattgc ctgcttacc acatctcgt ttttactct cgacaactg tggcacttt agaaacagt tatattgta attactaat ggtaacatt gttgttcac gcgtctttt tcttacagt tcttccagt catgacaat gacgtgatt tgtgaatgt agttattta taagtatat ttaaaatta tcaaatatg tacctattt ctccaagcg gtgtaatct tgacgtgag ccacacctc tcaaaagaa atatttttt aagattgtg gcagtgtta atattcaaa ttgttccca aaacgatag tgtttcaca cagaaagcc actcaactg tactactgc ccgctcaag actcctaaa ttgtcagct tttctaatc gatctacaa cactaaaca acacaatct ggcccggct tttgtgtga aataaccac aaccgaact tatttttaa acaatgtcg gttgttcaa gtcaaaaaa tcactttgc tgtgtgttg gacaagtta aggaattac ttctttgtt ggatttctt aaatgttga tcctgtcta aattaattt aagttatta attaatatt gttattaat tccgttaca tatagttta tttttgtta ttgcgatat ctttcacat tgccatttt gcatccaac cactattac gaatttaat aattcttta ctatttttt taaaggaat taaaattaa ttgttggag ggttggtgt aaggggcat tacgtatct gtaagtgca tgtgacatt ctagtcatt tgtaacaaa tgctatcta gtctcataa tgtatggtc taagtgatc tttttgata taatctgtt tactgttag aatctgata tgtattatt cttgttgta accattact tattcatgt attataa
