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Gene information
General information
TC003016 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 4466474..4468251
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003016
    iBeetle screen iB_00501

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 7 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003016 RA
      MDIRPNNTI YINNLNEKI KKEELKKSL 
      YAIFSQFGQ ILDIVALKT LKMRGQAFV 
      IFKEIQSST NALRSMQGF PFYDKPMRI 
      QYAKTDSDV ISKMKGTFQ ERPKKIRPP 
      PSKEDEAPK KKKKNKDPN RIPGGSNAE 
      QPPNQILFL TNLPDETSE MMLSMLFNQ 
      FPGFKEVRL VPNRHDIAF VEFENELQS 
      GAAKDALQG FKITPTHAM KISFAKK
    CDS sequence
    • >TC003016 RA
      atggatatt cgaccaaat aacacaatc tacatcaac 
      aacctaaac gagaaaatc aagaaagaa gaactaaag 
      aagtcactc tacgccata ttttcccaa tttggccaa 
      atcctggac attgtggcc ctcaaaacc ctcaaaatg 
      cggggccaa gctttcgtc attttcaag gaaattcaa 
      agctcgacc aacgcttta cgctccatg caggggttc 
      ccgttttac gacaaacca atgcgaatt caatacgca 
      aagacagat tctgacgtt atttcgaaa atgaagggt 
      acgttccag gagaggccc aagaaaata cgccccccg 
      ccttccaag gaggacgaa gcgcctaag aaaaagaag 
      aagaacaag gaccccaac agaatcccc ggtggaagc 
      aacgctgaa caacctcca aaccaaatt ctcttcctt 
      actaatctg cctgacgaa acgagcgaa atgatgttg 
      tctatgctt ttcaatcaa ttccctgga ttcaaagaa 
      gtgcgtctt gtgcccaac agacatgac atcgctttt 
      gtggagttt gaaaacgag ttgcagtca ggggctgcc 
      aaggacgcc ttgcaaggg ttcaagatc acacccaca 
      catgccatg aaaatatcc ttcgcaaag aaataa
    mRNA sequence
    • >TC003016 RA
      agcagactt gtcggatag atgtcagcg caatatcaa 
      acaggaagt taatcgacg ccatttttg tagccgacc 
      acctaaagc tttgaagtc aaacaacaa tgattcaat 
      ctaaagatt cagctggtt tattataca aagtttggt 
      gtttgaatc cattgtcgg cgttgaaac gttttgtga 
      ttttccata atttcgact taagtacaa aacaaatta 
      gactggaga agtttaatt ccaagaaaa cgtgctaat 
      ccgatcaga tttatttac caaacaaga agtattctg 
      ctaagaact agctgagat aactaacct caaaccatg 
      gatattcga ccaaataac acaatctac atcaacaac 
      ctaaacgag aaaatcaag aaagaagaa ctaaagaag 
      tcactctac gccatattt tcccaattt ggccaaatc 
      ctggacatt gtggccctc aaaaccctc aaaatgcgg 
      ggccaagct ttcgtcatt ttcaaggaa attcaaagc 
      tcgaccaac gctttacgc tccatgcag gggttcccg 
      ttttacgac aaaccaatg cgaattcaa tacgcaaag 
      acagattct gacgttatt tcgaaaatg aagggtacg 
      ttccaggag aggcccaag aaaatacgc cccccgcct 
      tccaaggag gacgaagcg cctaagaaa aagaagaag 
      aacaaggac cccaacaga atccccggt ggaagcaac 
      gctgaacaa cctccaaac caaattctc ttccttact 
      aatctgcct gacgaaacg agcgaaatg atgttgtct 
      atgcttttc aatcaattc cctggattc aaagaagtg 
      cgtcttgtg cccaacaga catgacatc gcttttgtg 
      gagtttgaa aacgagttg cagtcaggg gctgccaag 
      gacgccttg caagggttc aagatcaca cccacacat 
      gccatgaaa atatccttc gcaaagaaa taagaccac 
      aaattacgt aaataatta taaggaaca gttttataa 
      tttaacaat cagtcataa acaattttt cagttctga 
      agctccgtt cttgatgta ttcgtgtga caaaacgat 
      ttgtttctt ggctatttg tgatttttc ctcattttt 
      gcagccact tcaggtcac actttttta taaaatttg 
      caacgtttc gttagccca cacttccta aaaaagttt 
      aaattaatg ctaaaataa aaggaaagt ttgcgcctt 
      taggttatc tgagttttc gcacaccgt gtaaaataa 
      aatttaatt tatttgtgg tatatttac cctaataca 
      aattatttt cttcaccat gtctattct atgcttcaa 
      attttacac aaataatta taattaaaa catcatggt 
      ggaaaaaaa attacaaat tataatgga cttgcacta 
      caattggta caattgtga aactaaaac ctcaaaata 
      attcttaca ttttgaaat tacagtaca acttcttta 
      atccgaaca gaatttttt gttgcagtc aaatctata 
      ctacgaatg ttaggatca aaaacttgg aaaaacaat 
      tggttattg cttttaaat taataaatt ttaattaat 
      acttgaaaa aaaccttaa cattaaaac gataatata 
      ctttttttt ataaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY